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Biomedical subjects

S M Ali

Publications and source records attributed to S M Ali.

At least 91 records · Page 5Linked to original sources
PubMed1994

Evaluation of hospital diets in five hospitals at Dhaka city.

The study was undertaken to assess the prevalent situation of diets provide to different types of patients in five hospitals at Dhaka. Calorie content of foods supplied to the patients was about 50% of the calorie that was mentioned on diet menus for different hospitals. The patients intake of food supplied by the hospital was very low. Except BIRDEM, majority of the patients in other hospitals were not satisfied with different types of foods supplied to them by the hospitals. Calorie, carbohydrate, protein, vegetable fat, iron and calcium intake by the patients differ significantly (p = 0.03-0.001) in different hospitals. The hospital diet is imbalanced to met therapeutic need.

Bangladesh↗
PubMed1993

The Alzheimer beta-amyloid protein precursor/protease nexin-II is cleaved by secretase in a trans-Golgi secretory compartment in human neuroglioma cells.

Alzheimer beta-amyloid protein precursor (beta APP) is expressed endogenously and abundantly by human neuroglioma (H4) cells. Its secretory processing has been shown to involve discrete proteolysis within the beta A4 region, thus preventing beta-amyloid formation, by an enzyme which has been referred to as 'beta APP secretase'. This cleavage results in secretion of a soluble N-terminal 135 kDa protein and retention of an integral membrane C-terminal fragment within the cell. The membrane-associated C-terminal fragment is sorted to lysosomes where it undergoes limited degradation. We show here that most newly synthesized beta APP is degraded via a non-lysosomal pathway before maturation in H4 cells, and most mature beta APP is processed predominantly by the so-called secretase. The rapid kinetics of appearance/disappearance of a cleaved 135 kDa protein within a microsomal fraction and the slow accumulation of this form in the extracellular medium indicated that secretase cleaves beta APP in an intracellular compartment. Low-temperature block (20 degrees C) was used to demonstrate that beta APP is cleaved within a late Golgi compartment after sulphation which occurs in the trans-Golgi network (TGN). This is consistent with (1) the immunolocalization of most of the beta APP within a Golgi compartment that reacts with wheat germ agglutinin, (2) the fact that less than 1.5% of the total mature full-length beta APP is present at the plasma membrane and (3) subcellular fractionation studies which showed that the mature full-length and intracellular cleaved beta APPs co-sediment with a membrane fraction that is slightly more dense than the plasma membrane. This study provides evidence that most of the beta APP secretase in H4 cells is intracellular, and confirms that the resulting C-terminal fragment is delivered to lysosomes immediately after cleavage. These results are discussed with regard to the possibility that mature full-length beta APP escapes secretase cleavage and is delivered directly from the TGN to the lysosome without passing through the plasma membrane. Either pathway will result in the generation of amyloidogenic fragments.

Amyloid Precursor Protein Secretases↗
PubMed1993

Prediction of the service life of dental porcelains by the measurements of post-indentation slow crack growth.

Statistical fatigue life for body dental porcelains, cooled in a furnace and in air, was predicted from measurements of post-indentation slow growth of radial cracks in water and in air. Cracks were induced in the surfaces of porcelain discs by a Vickers indenter at an applied load of 9.8 N. Crack lengths were measured at various times over a period of 5 months to calculate the stress corrosion susceptibility coefficient, n. Lifetime under service stress was estimated from the measured value of n. The ratios of service stress to fracture strength were calculated as 0.47 and 0.69, respectively, for glassy and feldspathic porcelains which were cooled in a furnace and aged in water. The corresponding ratios for tempered (cooled in air) specimens were 0.58 and 0.67, respectively. These were estimated values when service life exceeds 10 years. The results suggest that the air cooling process can effectively increase the service life of glassy porcelains, but, for feldspathic porcelain it is less effective.

Corrosion↗
PubMed1993

Plasma level of antioxidant nutrients (retinol and alphatocopherol) in cases with different grades of cervical carcinoma.

A study was conducted on 23 patients on various grades of cervical cancer, selected purposely from Dhaka Medical College Hospital. Vitamin A and E level of this histologically proved cases showed mean serum level of vitamin A was 0.41 +/- 0.19 and that vitamin E was 4.21 +/- 2.15, and these low level was constant in all Histopathological grading of cervical cancer. Contraceptive user and smoker had very low level of vitamin A and E. Majority (51%) of the cases, were from lower middle class and 49% were from lower class, seventy seven percent were illiterate, 97% were married before 18 years, 57% had more than 6 children, 71% did not use contraceptive. The availability of vitamin A rich food were within reach of lower income group, than the food source of vitamin E which are usually of animal origin. It is concluded that in cervical cancer had low level of antioxidant vitamins A and E. Further study is indicated for understanding detail antioxidant nutrients role in aetiopathology of cervical cancer in Bangladesh.

Adult↗
PubMed1993

Isolation, purification and ultrastructure of bacteriophages from natural mosquito breeding places in Egypt.

Two bacteriophages were isolated from field collected samples representing two different mosquito breeding places. The phage AB-1 (isolated from Abheit village, Faiyoum Governorate "seepage water") and the phage GA-2 (isolated from El-Gabal El-Asfer Qualyobia Governorate "sewage drain water") were purified. Both bacteriophages were ultrastructurally described with respect to their morphology, dimensions, phases of bacterial attack and lysogeny. No major differences were observed between both isolated phages in relation to specificity, however; they were isolated from two different types of breeding places and two different geographic areas as well. This study may assume a wide host range of the isolated phages and reflect how bacterial insecticides used for mosquito larval control could be inhibited by such bacteriophage.

Animals↗
PubMed1993

Plasma LH and FSH levels in azoospermia and in normal male and female human subjects.

Immunoassay of human plasma LH and FSH level was carried out in the Institute of Nuclear Medicine, Bangladesh. Apparently normal male and female volunteers and subjects having primary sterility were studied. Plasma LH and FSH levels of normal males ranged from 1.9 to 20.48 (mean 7.3) and 1.17 to 6.75 (mean 3.30) m IU/ml respectively. Corresponding values for females were 0.99 to 38.92 (mean 17.94). Level of LH and FSH in azoospermic males were found higher than normal ones with the mean value of 17.0 and 5.67 m IU/ml respectively. The study gives an impression about the plasma LH and FSH levels in azoospermia and normal population in Bangladesh.

Female↗
PubMed1993

Comparative inhibitory action of 4 standard bacteriophages on the larvicidal activity of certain mosquito pathogenic bacteria against Culex pipiens larvae.

Four bacteriophage (CP-51, CP-54, Yousten-4 and Yousten-14) were assayed against 7 entemopathogenic bacterial strains. The two CP ones, indicating variability of the host range of the tested phages which was suggested to be related to the environmental characteristics of the tested strains. On testing the susceptibility of 5 bacterial strains to the phage Yousten-4 at different cultural ages, a correlation was found between incubation time and level of bacterial susceptibility to the tested phage. This observation was explained to be due to the number of vegetative cells and/or sporulation.

Animals↗
PubMed1993

A field survey of bacteriophage contamination of mosquito breeding places, inhibiting bacterial insecticide.

Twelve geographically different mosquito breeding places were described and sampled for the detection of naturally existed bacteriophage viruses which could transduct and lysate 5 entomopathogenic bacteria. The surveyed places are classified into seepage, sewage, and irrigation breeding water. Bacterial free filtrates of the collected samples were assayed against the tested bacteria in vitro and against 3rd instar Culex pipiens larvae as well. Nine out of the twelve places could demonstrate the presence of phages. Bacillus thuringiensis H-14 was foud susceptible to phage(s) present in polluted and irrigated water of 5 location, while, B. thuringiensis Berliner was susceptible to only a specific phage of one breeding place (polluted, sewage water). With regard to Bacillus sphaericus strains 1593 and 114, bacteriophages of sewage and irrigated water could lysate them and these phages are characterized by being of a moderate host range, except one phage which showed high specificity with strain 114 and was detected in a polluted sewage water sample collected from Dakahliya Governorate. The detected phages proved to lysate both B. thuringiensis H-14 and B. sphaericus 1593 on their larvicidal action through a series of bioassay experiment, almost all results indicate the presence of a significant inhibitory activity.

Animals↗
PubMed1993

Effect of bacteriophage lysogeny on the efficacy of two bacterial larvicide formulations under field conditions.

Two types of man-made ditches were selected for carrying out this experiment; one polluted with nitrogenous matters (sewage water) and second filled with accumulated irrigated clear non-chlorinated water. No phages were detected in samples collected from both types of ditches. However, phage(s) specific to only B. sphaericus was (were) detected after spraying the two types with both B. thuringiensis H-14 and B. sphaericus commercial formulations. The detection of phage(s) was observed after 3 days post-spraying in the polluted ditch and after one week in the non-polluted one. This observation was explained by a possible transduction of naturally existed phage(s) on other spore forming bacteria to the sprayed B. sphaericus only, as its commercial formulation is based on spores which germinate to produce vegetative cell, while B. thuringiensis H-14 contains only the O-endotoxin as active ingredient, and also due to the increase of the number of bacteria in the sprayed ditches due to the recycling of B. sphaericus in the aquatic breeding places.

Animals↗
PubMed1992

[Exercise echocardiography. A new valuable method for demonstration or exclusion of myocardial ischemia].

The aim of our study was to assess the diagnostic value of exercise echocardiography compared with exercise ECG and coronary arteriography. Exercise echocardiography was performed in 60 consecutive patients referred for coronary arteriography because of suspected coronary artery disease. Echocardiography was carried out in combination with bicycle exercise ECG and was performed 1) at rest before exercise with the patient supine, 2) at peak exercise on the bicycle and 3) immediately after exercise in supine position. Conventional standard views were recorded by means of an image computer. For the regional wall motion analysis, images were displayed simultaneously from the rest, peak and post exercise situations in a quadscreen format. Ischemia was diagnosed when wall motion deteriorated from rest to peak or post exercise in at least one out of a total of 16 left ventricular segments. Analysis was made without knowledge of patient data or results of exercise ECG or coronary arteriography. No patient was excluded because of reduced echocardiographic image quality during exercise or other technical reason. Coronary arteriography was performed in all patients and used as reference. The sensitivity of exercise echocardiography was 91% and the specificity 100%. The predictive value of a positive test was 100% and the predictive value of a negative test 78%. For exercise ECG the corresponding values were 58%, 100%, 100% and 39% respectively. We conclude that exercise echocardiography seems to be useful for the diagnosis and exclusion of ischemic heart disease. This and other studies indicate that the method is more sensitive than exercise ECG.

Adult↗
PubMed1992

Alternate scenarios for population control in Pakistan: the issue of contraceptive method mix.

The authors review Pakistan's population program, with a focus on the ideal mix of contraceptive methods needed to slow population growth. "Our objective in this exercise is to estimate the extent of services required to achieve a certain level of the Contraceptive Prevalence Rate (CPR) necessary to bring fertility down to a level desired by women." Comparison is made between single- and multiple-method approaches.

Asia↗
PubMed1992

Detection of early xerophthalmia by impression cytology and rose Bengal staining--a comparative study.

Twenty two cases were studied for early detection of Xerophthalmia by impression cytology and Rose Bengal staining. Patients were below 6 years of age and admitted in the paediatric ward of Dhaka Medical College Hospital. Assessment were done by conjunctival impression cytology and staining with 1% Rose Bengal dye. Estimation of serum retinol level was done by HPLC to correlate conjunctival findings with biochemical status. The mean age of the subjects were 3.15 +/- 1.75. Rose Bengal staining was positive in 40% cases and impression cytology was in 60% cases. The mean serum retinol level of the diseased children were 10.5 +/- 3. ug/dl. The sensitivity of Rose Bengal staining was 53.3% and that of impression cytology was 80% in detecting vitamin A deficiency when compared with serum retinol level. The specificity of Rose Bengal staining was 40% and that of impression cytology was 100%. Sensitivity of impression cytology was 100% in patient with retinol level 10 ug/dl.

Child, Preschool↗
PubMed1992

Role of vitamin A and E in spondylosis.

A biomedical study was undertaken at the Institute of Nutrition and Food Science, University of Dhaka to observe the effect of the antioxidative roles of vitamins A and E on the degenerative process occurring in spondylosis in human. A single and a double blind study were done. Thirty two patients suffering from spondylosis were selected for the study on the basis of certain criteria. The serum vitamin E level in most of the patients were lower (5.8 +/- 2.7 umol/L) than normal (11-41 umol/L). Vitamin E administration at a dose of 100 mg daily for three weeks resulted in a significant increase in serum vitamin E level accompanied by complete relief of pain. The serum vitamin A level of the patients were already within the normal (0.53-2 umol/L) range and vitamin A administration was not effective in relieving the pain. The results therefore strongly indicate that vitamin E is effective in curing spondylosis and most probably due to its antioxidant activity.

Adult↗
PubMed1992

Role of thymectomy in myasthenia gravis.

Thirteen patients with myasthenia gravis underwent total thymectomy between January, 1988 and December, 1991. The duration of symptoms prior to surgery varied from 2 months to 20 years. In a follow-up ranging from 2 months to 4 years, 11 patients showed a significant improvement with either complete discontinuation of medication or a marked reduction in doses. One patient with a small benign thymoma showed some improvement but subsequently required stepping up of anticholinesterase medication and addition of steroids and immunosuppressants; another patient with atrophic thymic tissue had complete remission after thymectomy but developed myasthenic symptoms six months later requiring medication again. Thymectomy is recommended for all patients with generalised myasthenia gravis with or without thymoma regardless of the duration of disease unless the patient is a very high risk candidate for surgery. It is not recommended for isolated ocular myasthenia gravis.

Adolescent↗
PubMed1991

[Reversible regional myocardial ischemia in variant angina].

A case of severe ventricular ischaemia induced by hyperventilation which occurred in a woman aged 44 years is presented. The ischaemia was confirmed by echocardiography and scintigraphy and coronary arteriography revealed spasm in the proximal segment of the anterior descending branch of the left coronary artery.

Adult↗