Biomedical subjects

S Li

Publications and source records attributed to S Li.

At least 1,351 records · Page 75Linked to original sources

PubMed1995

Vasoactive intestinal peptide receptor scintigraphy.

UNLABELLED: This study presents the biodistribution, safety and absorbed dose of 123I-VIP administered to 18 patients with intestinal adenocarcinomas or endocrine tumors. METHODS: To achieve high-specific activity, 123I-VIP was purified by HPLC. Following intravenous administration of 123I-VIP (172 +/- 17 MBq (4.65 +/- 0.5 mCi); < 300 pmole ( < 1 microgram)/patient), sequential images were recorded during the initial 30 min. Thereafter, whole-body images were acquired in anterior and posterior views at various time points. Dosimetry calculations were performed on the basis of gamma camera data, urine, feces and blood activities. RESULTS: After injection of labeled peptide, the lung was the primary site of 123I-VIP uptake. Peak lung activity represented 40% +/- 7% of the injected dose at 0.7 hr and declined to 21% +/- 7% at 3.5, to 14% +/- 3% at 7 and to 8% +/- 4% 22 hr postinjection. Radioactivity was excreted into the urine and amounted to 37% +/- 16% of the injected dose within 4, 68% +/- 12% within 8, 82% +/- 16% within 16 and 93% +/- 8% within 24 hr postinjection. The mean effective half-life of 123I-VIP in the lungs was 2.2 and 6 hr in the urinary bladder. The highest radiation absorbed doses were calculated for the lungs [67 muGy/MBq (248 mrad/mCi)], urinary bladder [77 muGy/MBq (284 mrad/mCi)] and thyroid gland [104 muGy/MBq (386 mrad/mCi)]. The effective dose was 28 muSv/MBq (104 mrem/mCi). CONCLUSION: HPLC-purified 123I-VIP shows favorable dosimetry and is a safe and promising peptide tracer for localization of tumors expressing receptors for VIP.

Adenocarcinoma↗

PubMed1995

[The posterolateral suboccipital approach to the lesions located at the anterior portion of the craniocervical junction].

A surgical approach to the anterior lower brainstem and upper cervical spinal cord consists of a fashion of unilateral suboccipital scalp flap, exposure and resection of the suboccipital bone and hemilamina of C1, removal of occipital condyle and the lateral mass of C1 (occasionally). The satisfaction of exposing the anterolateral portion of lower brainstem and upper cervical spinal cord can be obtained with minimal retraction of the nervous tissue. 13 patients, 9 with tumors, 2 with giant vertebral aneurysms, 2 with craniocervical malformations, underwent this operative procedure with no surgical mortality and morbidity which related to the approach.

Adult↗

PubMed1995

A two-year experience with laparoscopic cholecystectomy--a report of 1475 cases from Kunming, China.

Over a 2-year period, from 12 September 1991 to 11 September 1993, laparoscopic cholecystectomy was performed on 1475 patients with benign gallbladder disease in Kunming General Hospital, Yunnan, China. Of these, 28 cases (1.9%) were converted to open surgery. Various complications were documented in 27 instances including extrahepatic bile duct injury in 4 cases (0.3%), postoperative haemorrhage requiring laparotomy in 3 cases (0.2%) and bile leak from cystic duct stump in 1 case (0.07%). There was 1 (0.07%) death in the series. The junction between the gallbladder infundibulum and the cystic duct is an important landmark which laparoscopic surgeons must identify in the course of the procedure. Because the junction remains a comparatively constant landmark, in difficult laparoscopic cholecystectomy, excessive dissection of the bile duct would be unnecessary. During dissection of the hepatic hilus, blund dissection is recommended and the blind use of cautery and haemostasis should be avoided.

Bile Ducts, Extrahepatic↗

PubMed1995

[Human lens epithelial cell culture and its proliferative inhibition in vitro].

The cultures of human lens epithelial cells taken from both normal and cataractous lenses were established in vitro. The biological characteristics and the histological changes of the cells were investigated. 5-Fluorouracil (5-Fu), Homoharring tonine (HH) and Harmeline (H) were used for the inhibition of the proliferative cells in the experiment. The results revealed that the in vitro survival capacity of the human lens epithelial cells is limited and the proliferative capacity of the cells is negatively correlated to the donor age. HH and H can effectively inhibit the lens epithelial cell proliferation. Based on the results of this report, performing small and smoothly edged anterior capsulorhexis in extracapsular cataract extraction may possibly decrease the incidence of the post-operative opacification of the posterior capsule.

Adolescent↗

PubMed1995

[On the diagnosis of multiple myeloma an analysis of 2,547 domestic cases].

Analysis of 2,547 cases of multiple myeloma (MM) reported in China in 1980s showed that the clinical manifestations are characterized by multipliey. High misdiagnosis rate (69%) and multiple complications. While the monoclonal protein had more than 25 immunological type, IgG myeloma was the commonest (43.1%). Light chain subgroup trended to have higher incidence of renal damage (76.9%). Plasma cell leukemia eventually developed in 30 cases. In order to improve diagnosis and avoid misdiagnosis, the key points are 1, to better the recognition of clinical features of MM. 2. patient should receive urine Bence-Jones protein, immunoglobulins, immunoeletrophresis, bone X-ray and multiple site bone marrow puncture whenever one of such manifestations as unexplained anemia, skeletal pain, proteinuria, elevation of ESR, hyperviscosity syndrome, hypercalcemia, hyperuricemia, elevation of alkaline phosphatase, pathological fractures and diffuse osteoporosis. 3. immuno-binding electrophoresis and immunofluorence antibody detection should be done for suspected cases with normal immunoglobulin level.

Adolescent↗

PubMed1995

[Radiolabelling and assay of Chinese agkistrodon acutus venom with carrier-free Na 125I].

Chinese agkistroden acutus venom (CAAV) was radiolabelled with carrier-free Na 125I by the method of Iodogen. The specific activity and radiochemical purity for radiolabelled products were 4236.5 x 10(10) Bq/mmol and 98%, respectively. Each CAAV molecule carried 0.52 125I atom. Physical and chemical characterization of radiolabelled CAAV was similar to unradiolabelled CAAV. Binding analysis showed that 125I-CAAV was bound to platelet in a saturable manner. Binding sites per platelet were 13,255 +/- 6292/platelet. The dissociation constant (Kd) was 3.2 +/- 0.69 x 10(-10) mol/L. These results are similar to binding sites of other snake venom on platelet. The investigation showed that radiolabelled CAAV made by our laboratory was useful for radioligand binding assay.

Agkistrodon↗

PubMed1995

[A preliminary study on the antenatal diagnosis and prevention of the fetus toxoplasmosis infection].

For those pregnant women with an abnormal pregnancy history, the polymerase chain reaction (PCR) technique was adopted to screening the serum toxoplasmosis DNA (TOX-DNA). Then tests were made for further evidence of TOX-DNA in the amnionic fluids TOX-DNA was examined in cases with blood TOX-DNA positive. Gamma glutamyl transpeptidase (GGT) and alpha-fetoprotein (AFP) in the amnionic fluids were measured for reference, a antenatal diagnosis of the fetal congenital toxoplasmosis infection could thus be made. In the present paper, 9 out of 92 blood specimen were TOX-DNA positive, of the 9 cases, 5 had TOX-DNA and 4 had no TOX-DNA in the amnionic fluid tests. The results of follow-up examination in the 9 cases were in conformity with the ante natal diagnosis. This is an important procedure for diagnosis and prevention of maternal-fetal vertical infection of Toxoplasmosis.

Animals↗

PubMed1995

[Analysis of 100 cesarean section with indication of cephalopelvic disproportion].

A retrospective analysis on the 100 cases cesarean section with indication of cephalopelvic disproportion was carried out in Beijing Obstetrics and Gynecology Hospital, From March to October in 1992, there were 73 cases were performed operation with correct diagnosis and management, another 27 cases were required further discussion, such as too early intervention of the delivery in laten phase in 8 cases, improper observation and management in active phase in 19 cases. Therefore we suggest that the diagnosis of cephalopelvic disproportion should be carefully after observation in labor stage and sufficiently trials of labor. It may decrease the rate of cesarean section and increase the obstetric quality.

Adult↗

PubMed1995

[Stability of angesin solution].

Chemical kinetics of ethyl acetate extract from the roots of Angelica sinensis in aguerus solution (angesin solution) was studied by isothermal acceleration test. The useful life (t0.9) calculated for angesin solution is one year.

Drug Stability↗

PubMed1995

A comparative study of imovane and estazolam treatment on sleep disturbances.

Twenty-six patients with sleep disturbances who had completed therapy with hypnotics were observed under single-blind and self-comparable method for 7 days each. We found that patients treated with imovane or estazolam had no statistical differences with respect to sleep time onset, total sleep time, nocturnal awakening, feeling after awakening, dream, drug side effects and sleep quality. Imovane performed better than estazolam in sleep time onset and total sleep time. From this study, imovane can be recommended as a new hypuotic to treat patients with sleep disturbances.

Adolescent↗

PubMed1995

[The role of platelet activating factor in mesenterioangial microcirculatory disturbance complicated with acute pancreatitis in rats].

To study shock in early acute pancreatitis (AP), we observed the change of mesenterio-angial microcirculation in the rat with AP induced by sodium taurocholate infused into the pancreatic duct. Meanwhile, the effect of platelet activating factor (PAF) receptor antagonist BN52021 upon the animal with AP was also investigated. 18 Sprague-Dawley rats were divided into three groups: pancreatitis group pan + BN52021 group and control group. pan + BN52021 group was injected BN52021 (10mg/kg) intravenously 5 minutes and 180 minutes after AP induction. The other groups were injected normal saline. We also studied the survival times of rats. The results showed slow mesenterio-angial small arteriolar and venular blood flow velocity and dilated meseaterio-angial small venular diameter during the early phase of AP. An protective effect of BN52021 on mesenterio-angial microcirculation injuries accompanied by AP was confirmed Animals treated with BN52021 survived 458.3 +/- 9.5 minutes whereas death occurred 243.3 +/- 2.3 minutes after AP induction in untreated rats (P < 0.01).

Acute Disease↗

PubMed1995

[Patella resurfacing and patellofemoral resurfacing].

Since 1983 isolated polyethylene patellar prosthesis was used for patella resurfacing of 19 patients with patellofemoral osteoarthritis. After an average of 44.9 months follow-up, the treatment was found to be not as satisfactory as earlier trials. The main reason was the wide erosion of femoral condyle caused by the polyethylene patella. To overcome this shortness, a new type of patellofemoral prosthesis was designed by the authors, which was named Y-L-Q. From January, 1991 to November, 1993, this prosthesis was used to treat 16 knees of 13 patients with patellofemoral osteoarthritis. Most of those patients improved both symptomatically and functionally. The good to excellent results rate was 87.5% (14/16 knees) at the time of an average 10 month follow-up. The early results of our experience with patellofemoral resurfacing are encouraging, and extended follow-ups are in progress.

Aged↗

PubMed1995

[Gonioscopic observation after posterior chamber intraocular lens implantation].

6 months after senile extra-capsular cataract extraction and posterior chamber intraocular lens (IOL) implantation, Goldmann gonioscopy was performed on 30 eyes having undertaken the surgery. Peripheral anterior synechiae (PAS) were found in 17 eyes (56%). PAS overlying the positions of 20 lens haptics were observed in 15 eyes. It is easier to develop PAS in eyes with vertically fixed haptics than in eyes with horizontally fixed ones. The causes and the consequences of PAS were discussed. It is suggested that during the follow-up, routine gonioscopic observation after posterior chamber IOL implantation be necessary.

Adult↗

PubMed1995

Clinical observation on 80 children with peptic ulcer treated primarily by traditional Chinese medicine.

Using the empirical recipe of Wang Pengfei (a very experienced TCM doctor) as the main treatment, the authors treated 80 children with peptic ulcer by means of promoting blood circulation, removing blood stasis as well as regulating the flow of qi, alleviating pain, tonifying the stomach and reinforcing the spleen. Measures for hemostasis, correction of anemia and other general treatment were also taken. After 8 weeks of treatment, a total effective rate of 92.5% was obtained.

Adolescent↗

PubMed1995

[Efficacy of praziquantel combined with albendazole in the treatment of clonorchiasis].

This paper reported on the clinical efficacy of praziquantel-albendazole combination in the treatment of clonorchiasis in Guangxi. Two hundred and two cases were divided into three groups. The first group comprised 66 cases who were treated with praziquantel at 180 mg/kg bwt for three days. The second group consisted of 62 cases who were treated with albendazole at 90 mg/kg bwt for three days. In the third group, 74 cases were treated with 90 mg/kg bwt praziquantel plus 45 mg/kg albendazole for three days. The results showed that the negative conversion rates were 98.5%, 61.3% and 87.8% in groups one, two and three, respectively, one month after treatment. In some cases who continued to be egg positive, the reduction rates in faecal egg counts were 99.7%, 65.0% and 97.3%, respectively. Although the cure rate in group three was not as high as in group one, the side effects were more mild and transient and the cost of treatment could be reduced by 60% as compared with group one. In cases with light and moderate infections, the praziquantel-albendazole combination proved more effective. In addition, the praziquantel-albendazole combination was also highly effective for treating those cases who were infected with Ascaris, Trichuris or hookworm.

Albendazole↗

PubMed1995

[The effect of measuring the curvature of soft palate to determine the position of posterior peripheral seal].

This study was undertaken to determine the feasibility of the surveyor of curvature in fabricating a full upper denture. 48 cases were investigated to determine the position of the posterior peripheral seal with the instrument. The results showed that the retention of the full upper denture was greater than that of the control-team. This method is more suitable for the patients with lower flat or higher palatal vault.

Anthropometry↗

PubMed1995

Effect of intracerebroventricular beta-funaltrexamine on mu opioid receptors in the rat brain: consideration of binding condition.

Effects of 24 h pretreatment with intracerebroventricular (icv) beta-funaltrexamine (beta-FNA) on brain opioid receptor binding in rats were examined under various conditions. Agonist binding to mu and delta opioid receptors (with [3H][[cap]dAla2,MePhe4,Gly-ol5]enkephalin (DAMGO)[3H][D-Pen2, D-Pen5]enkephalin (DPDPE), respectively) was performed under three different conditions: i) pretreatment of membranes with GDP and Na+ and binding in the presence of Mg++ in Tris-HCI buffer containing EGTA and leupeptin for 1.5 to 3 h; ii) binding in Tris-HCI buffer containing bacitracin, leupeptin, chymostatin and bestatin for 3 to 4 h; iii) binding in Tris-HCI buffer containing EGTA and leupeptin for 45 min. Condition i was shown to convert opioid receptors to a high affinity state for agonists. beta-FNA (2, 6 or 20 nmol) significantly reduced 1 nM [3H]DAMGO binding in the whole brain with i but not with ii. With iii, 20 nmol beta-FNA reduced [3H]DAMGO binding, but not 2 or 6 nmol. Saturation experiments with i showed that the reduction in [3H]DAMGO binding after 6 or 20 nmol beta-FNA was due to a decrease in Bmax and an increase in KD. For delta binding, there was no significant change in [3H]DPDPE (2 nM) binding with i after 2, 6 or 20 nmol beta-FNA. Thus, under i, icv beta-FNA reduced [3H]DAMGO binding significantly without affecting [3H]DPDPE binding. In addition, mu binding was also conducted with 1 nM [3H]naloxone under three different conditions: iv) in the presence of Na+ and GDP; v), in the presence of Na+, Gpp(NH)p and Mg++; vi) in the presence of Na+. Both iv and v were shown to shift opioid receptors to a low affinity state for agonists. beta-FNA (20 nmol) significantly decreased 1 nM [3H]naloxone binding under each of the three conditions. Competitive inhibition of 1 nM [3H]naloxone binding by DAMGO in the presence of Na+ and GDP showed that receptors existed in a single low affinity state for DAMGO, and that icv beta-FNA caused a reduction in Bmax without affecting the KD of DAMGO. In summary, when all the receptors were converted to a high agonist affinity state i or a low agonist affinity state iv, the changes in mu binding induced by beta-FNA could be revealed with agonist binding. Additionally, changes in mu binding induced by beta-FNA could be detected with [3H]naloxone, which always displayed high affinity regardless of agonist affinity states, under each of the three conditions (iv, v and vi).

Analgesics↗

PubMed1995

Value of polymerase chain reaction assay in diagnosis of intestinal tuberculosis and differentiation from Crohn's disease.

It is difficult to make a precise diagnosis of intestinal tuberculosis and to differentiate it from Crohn's disease. For evaluating Polymerase Chain Reaction (PCR) assay in these two aspects, 36 specimens of intestinal tuberculosis from surgical resections and endoscopic biopsies and 26 Crohn's disease samples were subjected to PCR assay. 21 specimens of normal colon tissue surrounding cancer were used as the control. Oligonucleotides derived from the IS 6110 sequence, which is repeated in M. tuberculosis chromosome and highly specific for the M. tuberculosis complex, were used as a primer. The amplified PCR products were detected by examination of ethidium-bromide-stained polyacrylamide gels. The specificity of PCR products was confirmed by digestion with Sal I restrictive endonuclease and southern blot hybridization using digoxigenin-labeled probe. The results showed that the M. tuberculosis DNA was identified in 27/36 intestinal tuberculosis, but none of 26 Crohn's disease. Acid fast bacilli were only found in 16/36 intestinal tuberculosis. In conclusion, as a rapid, sensitive, and specific pathogenic method in diagnosis of intestinal tuberculosis, PCR assay has been developed in this study, and is considered valuable in the differentiation between intestinal tuberculosis and Crohn's disease.

Base Sequence↗