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Biomedical subjects

S Li

Publications and source records attributed to S Li.

At least 955 records · Page 53Linked to original sources
PubMed1998

[Studies on microbial factor on color change of Dunhuang mural. I. Classification of microbes on color-changed mural and property of some typical species].

Microbial strains were isolated from 51 typical color changed samples of mural in 6 grottos of Dunhuang Mogao Gretto. After identification, it is proved that belong to 6 genera of bacteria and 5 genera of mould. Bacillus, Alcaligenes and Penicillium were dominant species in all of them. In the imitative experiment, it is found that Cladosporium sp., A. niger and two strains of bacteria have a great effect on the color change of red pigment of mural and aging of sizing agent.

Bacteria↗
PubMed1998

[The biodegradation of trichloroethylene by a methanotrophic bacterium].

A Methylomonas (strain GYJ3) isolated in our laboratory was identified as the type II methanotroph on the basis of the intractytoplasmic membrane of the ultrastructure. The optimal culture conditions for production of the soluble from of methane monooxygenase (MMO) were determined, in which the ratio of methane to air in atmosphere was 2 to 1 and Cu2+ concentration was 1.5 mumol/L. The biodegradation of trichoroethylene(TCE) by the resting cells of the strain GYJ3 was studied. All experiments were performed with cells grown under above conditions and thus expressing soluble MMO. This results showed that TCE at the high concentration of 30 mg/L did not inhibit to the enzymes in the cells. Addition of formate increased the initial specific TCE degradation rates. The product of TCE oxidation was found to be toxic to the cells. The degree of inactivation of MMO was proportional to the amount of TCE degraded. The TCE degradation capacities(Tc) of resting cells was determined. In no-formate and formate-fed experiments, the TCE degradation capacities were found to be 0.0778 and 0.0851 mg of TCE/mg of dry cell, respectively.

Biodegradation, Environmental↗
PubMed1998

[Growth of Methylomonas Z201 cells and production of epoxypropane in monophasic and biphasic fermentation systems].

The growth of Methylomonas Z201 cells and production of epoxypropane in mono- and biphasic fermentation systems were studied. In monophasic fermentation systems, the inhibitions of propene and epoxypropane on the growth of Methylomonas Z201 cells were observed and the concentration of epoxypropane reached 1.3 mmol/L. In biphasic fermentation systems, hexadecane acted as the "reservoir" of growth substrate (methane) and reactants (propene, molecular oxygen), the decrease of propene and epoxypropane in aqueous phase reduced the inhibition effect of propene and epoxypropane on the growth of cells, the concentrations of epoxypropane in both water phase and hexadecane phase reached 1.7 mmol/L and 2.6 mmol/L. In both monophasic and biphasic fermentation systems, the operational stability of cells was enhanced compared to that of resting cells.

Alkenes↗
PubMed1998

[Effect of Limonium bicolor on bombyxmori life-span].

The effect of limonium bicolor decoction (LBD) (1%, 5%, 10%) on the life-span of bombyxmori was investigated. It was found that LBD could obviously prolong not only average life-span and average of highest life-span of both female bombyxmori and moth, but also the daration of female silkworm stage. Furthermore, LBD clud be antianoxiasis and hunger-resistant, these results showed that BLD could promise longevity.

Aging↗
PubMed1998

[Study of anti-tumor action and influence on immune function of Allium stativum].

Applying mice test on cancer model of S180 and Lewis cancer by using method clystered stomach(oral), observs the Anti-tumor function. Experiments showed that Allium sativum can inhibit tumor growth and Lengthen lift duration of mice, the rate of inhibiting tumor was 74.35% and life lengthening rate were 42.4%, with control grou comparison. There was profund difference between them. Besides, we discoved Allium sativum can raise immune function of mice.

Allium↗
PubMed1998

[Identification on commercial drug jiangxiang from Hongkong].

Accordint to macroscopical characters, histological structurs and analysis of essential oil, the comparison of drug Jiangxian from Hong Kong with Dalbergia sisso and Pterocarpus spp. was studied. The result showed that commercial drug Jiangxiang from Hong Kong is the heartwood of Pterocarpus masupium.

Chromatography, Thin Layer↗
PubMed1998

[Pharmacological studies of shundao granules on gastrointestinal track].

The traditional Chinese medicine Shundao Granules po markedly inhibited the formation of gastric ulcers induced by indomethacin, restraint and pyloric ligatin in rats. A decreased volume of gastric juice collected from pyloric ligated stomaches and a reduced level of gastric acid have been observed. The results showed that Shundao Granules had markedly analgesic action and significantly enhanced intestinal movement in mice, increased propulsive rate of small ingestion in mice.

Analgesics↗
PubMed1998

[Analysis of rhodopsin and peripherin/RDS genes in Chinese patients with retinitis pigmentosa].

PURPOSE: To disclose the mutation of rhodopsin and peripherin/RDS genes among Chinese patients with retinitis pigmentosa as there was no identified mutation through sequencing reported in Chinese. METHODS: Genomic DNA was prepared from the peripheral lymphocytes. Gene fragments of the rhodopsin and peripherin/RDS genes were amplified by the polymerase chain reaction. The PCR products were analyzed by Heteroduplex-SSCP technique. PCR samples with aberrant migrational bands were identified through direct sequencing or cloning sequencing. RESULTS: Three different mutations in the rhodopsin gene were found in 3 of the 83 patients with retinitis pigmentosa(Va1104Phe, Lys311Glu, Pro347Leu). Two of the three mutations have not been reported before. One of the two (heterozygous, Va1104Phe) was found in an isolated patient and the other (homozygous, Lys311Glu) in a family with autosomal recessive retinitis pigmentosa. Mutation of peripherin/RDS gene was not found in the 83 patients. CONCLUSION: Mutation in the rhodopsin gene is the common cause in Chinese patients with retinitis pigmentosa, either autosomal dominant, recessive or sporadic.

Base Sequence↗
PubMed1998

[Preparation of liposome encapsulated daunorbicine and determination of daunorubicine of aqueous humor in rabbit eyes].

PURPOSE: To investigate the method of determination the concentration of daunorubicine(DNR) and liposome encapsulated daunorubicine(LDNR) of aqueous humor in rabbit eyes. METHODS: After extracapsular lens extraction, 0.1 ml of 1 mg/ml DNA and LDNR were injected into the anterior chamber. At the 12, 24, 48 hours and 1 week after operation, the aqueous humor were aspirated to determine the concentration of DNR by high performance liquid chromatography(HPLC). RESULTS: At the 12, 24, 48 hours after operation, DNR were determined in all eyes, but at one week after operation, the DNR could only be detected in LDNR group. The concentration of DNR in LDNR group were higher than DNR group.

Animals↗
PubMed1998

[Observation of the effect of pediatric secondary posterior-chamber intraocular lens implantation].

PURPOSE: To evaluate the effects of secondary posterior chamber lens implantation for aphakia in children. METHODS: Thirty-six consecutive pediatric aphakic eyes were retrospected. All eyes were secondarily implanted posterior-chamber lens using different operative techniques according to the condition of lens capsule remained after dissecting the posterior adhesion, forming extent space in posterior chamber. Follow-up ranged from 1 to 20 months. The effect of corrected visual acuity was observed. RESULTS: Postoperative nated visual acuity was equal or better than corrected visual acuity preoperatively in 22 eyes(61.1%). Postoperative corrected visual acuity was better than that before surgery in 32 eyes (88.8%) and was equal to that before surgery in 4 eyes(11.1%). CONCLUSIONS: Secondary posterior chamber IOLs implantation was an effective surgery for aphakic eyes in children, but attention should be paid to anti-inflammatory treatment and amblyopia treatment.

Amblyopia↗
PubMed1998

Color vision defects with variation in the exon 5 of red and green pigment genes.

PURPOSE: To investigate correlation of variation in the exon 5 of red and green pigment genes with color vision defects. METHODS: Exon 5 of the red and green pigment genes in 11 protans, 19 deutans and 38 normal controls were analyzed by heteroduplux-SSCP analysis. RESULTS: In all 11 protans and 8 of the 19 deutans, defects of the red or green pigment gene could be identified. The C polymorphism (A/C at codon 283) in green pigment gene was present in 8 of 44 trichromats and 5 of 24 dichromats. Specific electrophoretic bands were found in 2 normal controls and a deutan. CONCLUSIONS: Variation in the exon 5 of the red and green pigment genes is the most common cause for color vision defects. Heteroduplex-SSCP analysis is a suitable way in screening specific variation in visual pigment genes.

Chromosomes, Human, X↗
PubMed1998

Studies on human fetal lens crystallins under oxidative stress and protective effects of tea polyphenols.

PURPOSE: To investigate the oxidative modification of water-soluble crystallins of human fetal lens with H2O2 and fourteen metal ions with or without EDTA. Tea-polyphenols (TP) was added to above solutions in order to testing their antioxidative abilities. METHODS: The experiments were performed at 37 degrees C with final concentration of 2.5 mg/ml protein, 0.1 mM metal ions, 0.3 mM EDTA and 1.0 mM H2O2. Then the TP was added to the solution with CuSO4 and H2O2, after 5 or 24 hours, the crystallins were analysed with SDS-PAGE and IEF. RESULTS: There were marked oxidative modifications of lens protein in H2O2 and copper without EDTA. In SDS-PAGE patterns, we found an increase in those species above of bands higher than 30 kD and some diffuse bands from 30 to 17 kD after 5 hours. In IEF patterns, there were a general increase on acidity with loss of the more basic species. When the TP was added, there was not any difference with control group. CONCLUSION: The results indicate that exposure of water-soluble protein to H2O2 and copper leads to covalent crosslinking and cleavage of polypeptides. After 24 hours, the development of the oxidative modifications of crystallins continues, comparison with the catalytic strength, copper ions stronger than the iron ions. On other hand, this work reported that the anti-oxidative action of TP is strong.

Antioxidants↗
PubMed1998

[The expression of human leukocyte antigen from peripheral blood of patients with herpetic keratitis].

PURPOSE: To understand the correlation between the expression of human leukocyte antigen from peripheral blood and herpetic keratitis so as to find the pathogenesis of HSK. METHODS: The expression of HLA-ABC, HLA-DR, HLA-DP, HLA-DQ from peripheral blood of 32 cases with herpes simplex keratitis (the stromatic and ulcer herpetic keratitis are 16 cases respectively), comparing with the normal people, were investigated by flow cytometry. RESULTS: The expression rate of HLA-ABC from peripheral blood of patients with herpetic keratitis (both the stromatic and ulcer groups) was lower, and the expression of HLA-DR and HLA-DP was higher than normal people. No difference was found between the stromatic and the ulcer herpetic keratitis in the expression of HLA-ABC, HLA-DR, HLA-DP, HLA-DQ. CONCLUSION: The immune model in patients with herpetic keratitis is different from normal people. The individual with this kind of immune model can not destroy the infected virus effectively, also show autoimmunization to cornea.

Adult↗
PubMed1998

[Study on human eye ciliary muscule cell culture and biologic characteristics].

PURPOSE: We cultured human ciliary muscle [HCM] cells to study their growth, ultrastructure, immunohistochemistry and functional characters. METHODS: HCM cells from 10 young donor eyes were cultured with collagenase IV digestion procedures in vitro, the cells were identified by eletronmicroscope and immunohistochemistry assay, their function were studied by single-cell-contraction assay. RESULTS: The cells were passed and grew in Hill-Valley pattern after conflunet; abundant filaments were presented under electronmicroscope. In Desmin protein immunohistochemistry study, the cultured cells were stained positive; in tissue sections, HCM cells stained positive, vascular smooth muscle stained positive weakly, but fibroblast cells and endothelial cells stained negative. 10(-3) M Carbachol could induce the cultured cells contract, this effect was antagonized by 10(-3) M Atropine. CONCLUSION: We successfully cultured HCM cells, which were able to contract.

Cells, Cultured↗
PubMed1998

Effect of cataract surgery on ocular axial length elongation in young children.

PURPOSE: To evaluate the influence of cataract surgery on eyes of children between 1-5 years old. MATERIALS AND METHODS: Forty-seven cases of pediatric cataract, including 31 cases of bilateral congenital cataract and 16 cases of unilateral posttraumatic cataract, were retrospected. Of the 31 cases of congenital cataract, 19 cases (group 1) had undergone cataract extraction with intraocular lens (IOL) implantation, 12 (group 2) had undergone cataract extraction without IOL implantation. Each of them had their two eyes operated separately at an interval of 1 year or longer. Of the 16 cases of posttraumatic cataract, 6 (group 3) had undergone cataract extraction with IOL implantation and 10 (group 4) had undergone cataract extraction without IOL implantation. Mean age at the time of interested surgery was 2.9 years (range from 1.1 to 5.1 years). Ocular axial length of both eyes measured by A-scan ultrasonography before and after the surgery of the earlier operated eye in the congenital groups and the operated eye in the post-traumatic groups was recorded. The elongation of axial length of the earlier operated eye in the congenital groups and the operated eyes in the posttraumatic groups of each case were compared with that of the fellow eye statistically with paired t-test. RESULTS: The mean follow-up was 2.6 years (ranged from 1.0 to 6.4 years). In all the 4 groups, the elongation of axial length between the earlier operated eye and later operated eye in the congenital groups and between the operated and unoperated eyes in the posttraumatic groups of each case was not significantly different (p > 0.05). CONCLUSION: Cataract surgery, either extraction with or without IOL implantation, did not retard axial elongation in children above 1 year old.

Age Factors↗
PubMed1998

Androgen therapy for menopausal women.

The cohort of menopausal women has dramatically increased in size. Along with the drop in endogenous estrogen during this transition, there is also a drop in endogenous testosterone. This drop in endogenous testosterone is particularly marked in women experiencing chemical and surgical menopause and can lead to diminished sexual desire and lack of feelings of well-being. The nurse practitioner is in an ideal position to identify these quality-of-life problems and provide appropriate androgen therapy as needed.

Adult↗
PubMed1998

[Studies on infrared spectra of Schiff base ligands and Mn(III) complexes with methylene bridge].

The FTIR spectra (3800-150cm(-1)) of Schiff base ligands and Mn(III) complexes with methylene bridge were measured and investigated. The major bands have been empirically assigned in comparison with a large number of previous results. The characteristic absorption bands of intra-molecular hydrogen bond of ligands were found at ca. 3438cm(-1). The vC=N of ligands and complexes were found at ca. 1630cm(-1). The characteristic absorption bands of the phenyl ring of ligands and complexes were found at ca. 1630cm(-1). The characteristic absorptin bands of the phenyl ring of ligands and complexes were found at ac. 1598, 1469 and 755cm(-1). The vibrations coupling between the M-N stretching modes and the phenyl ring deformation were found at ca. 369cm(-1). The vibrations coupling between the M-N deformation modes and the phenyl ring deformation modes were found at ca. 247cm(-1). The vibrations coupling between the M-O stretching modes and the phenyl ring deformation were found at ca. 329cm(-1). The vibrations coupling between the M-N deformation modes and the phenyl ring deformation modes were found at ca. 275cm(-1). The vMn-Cl of the complexes were found at ca. 303cm(-1). The vMn-N of the complexes shift to higher wavenumbers and the vMn-Cl shift to lower wavenumbers than of the phenylporphyrin Mn(III) complexes.

English Abstract↗