Search PubMed⌕ Search

Biomedical subjects

S Lenz

Publications and source records attributed to S Lenz.

At least 55 records · Page 3Linked to original sources
PubMed1987

Transfundal transfer of embryos using ultrasound.

Ultrasonically guided transfer of embryos was performed transabdominally in 10 patients. The uterus was punctured by a 16-gauge needle with a stylet through a full urinary bladder. When the needle tip was in the uterine cavity the stylette was removed and a transfer catheter loaded with the embryos was inserted. No complications or technical difficulties were experienced. None of the patients became pregnant.

Adult↗
PubMed1987

Evaluating the possibility of uterine transfer by ultrasonically guided transabdominal puncture.

Ultrasonically guided transvesical puncture of the uterine endometrial cavity was carried out in 16 patients to evaluate the feasibility of embryo transfer by this procedure. Instillation of fluid into the uterine cavity was achieved in all cases and easily performed in 15 patients. In one patient the fundal endometrium was reached in the third attempt. Instillation of fluid within the endometrium was attempted unsuccessfully in four patients, as the fluid leaked into the uterine cavity. In 12 patients 2-4 ml of fluid was injected. The fluid passed down through the cervix in patients in the luteal phase (nine patients) when 3 ml or more was injected. When a smaller amount of fluid was instilled it remained inside the cavity. In one patient in the preovulatory phase the fluid was actively pumped by uterine contractions into the vagina. Two cases in the proliferative phase showed no leakage from the cervix when 1.5 and 2 ml of fluid was injected, respectively.

Embryo Transfer↗
PubMed1987

Transvaginal recovery of oocytes for in vitro fertilization using vaginal ultrasound.

Vaginal recovery of oocytes for in vitro fertilization (IVF) was performed in 75 patients using a vaginal puncture transducer. Follicles were aspirated and flushed four times using a controlled vacuum. Oocytes were collected in 74 patients and an average of 5.1 oocytes was collected per patient. An average of 8.8 follicles was aspirated per procedure, giving an oocyte recovery rate of 58% per follicle. Pregnancy was obtained in seven patients (9.3%). The only complication was bleeding from the vagina of an estimated 40 ml in one case.

Cell Separation↗
PubMed1987

Are ultrasonic-guided follicular aspiration and flushing safe for the oocyte?

Oocyte collection was carried out in 53 patients by ultrasonically guided abdominal puncture under local or epidural anesthesia. Follicles were aspirated and flushed two to six times using a syringe with culture medium. A total of 196 oocytes was collected and 84 of those (42.9%) were found in the flushes. Mechanical damage was observed in 5.1% of the oocytes. Cleavage rates in mature oocytes (157) after 48 hr in culture were similar in the aspirate group (56.5%) and in the flush group (54.2%). Ten clinical pregnancies were obtained, corresponding to a pregnancy rate of 18.9%.

Female↗
PubMed1987

Ultrasound in detection of early neoplasia of the testis.

Testicular ultrasound scanning was performed in 192 men with a history of cryptorchidism. The ultrasonic tissue pattern was evaluated and each testis was accordingly given a score on a scale from 1 to 4, 1 representing the normal uniform pattern and 4 being a very irregular pattern. Testicular biopsy of the previously maldescended testicle(s) was carried out in 143 of the scanned men and from two contralateral testicles where the score 4 was given at the ultrasound examination. Two biopsies showed carcinoma-in-situ pattern. Both were found among testes with a very irregular echo pattern. This pattern was found in only 3.2% of all scanned testes.

Adolescent↗
PubMed1987

Testicular biopsy as an outpatient procedure in screening for carcinoma-in-situ: complications and the patient's acceptance.

Testicular biopsy has been performed as a simple outpatient procedure to facilitate screening purposes. The target population was men aged 20-30 years with previous cryptorchidism, and the aim of the study was to discover carcinoma-in-situ. When results from the first 209 consecutive males were assessed, very few and minor complications were encountered. Only three patients needed hospitalization for drainage of pus from the wound. The effects of the procedure as experienced by the patients were evaluated by a questionnaire. 72% of the patients found the method acceptable. The advantages were the patients' minimal absence from their jobs, and the low consumption of resources.

Adult↗
PubMed1986

Ultrastructure of the early human implantation in vitro.

Four hatched human blastocysts obtained after in-vitro fertilization and development were placed on monolayer cell cultures of human endometrial epithelium, and subsequently examined by transmission electron microscopy. All four blastocysts became adherent to the monolayer and three implanted and exhibited outgrowth of their trophoblastic cells. During implantation the blastocysts differentiated into mural and polar trophoblastic cells, and embryonic cells including endodermal cells. The endometrial cells were displaced and stacked into a multilayer at the periphery of the implantation sites, allowing the trophoblastic cells to come in contact with the culture dish. The endometrial cells displayed local exo- or endo-cytosis where they contacted the trophoblastic cells. The trophoblastic cells were not observed to be phagocytosing endometrial cells. These observations suggest that human blastocysts portray an intrusive type of implantation during the initial stages.

Blastocyst↗
PubMed1985

Ultrasonic study of follicular maturation, ovulation and development of corpus luteum during normal menstrual cycles.

Ovarian follicular growth, ovulation and development of corpus luteum was monitored with ultrasound in 29 normal menstrual cycles in 14 women. Results were correlated to changes in serum estradiol, LH and progesterone levels. The follicles reached a maximum diameter of 20.5 +/- 2.70 mm (mean +/- SD) with an intercycle variation of 16 to 25 mm, and growth rates ranged between -2 and 6 mm from the day before the LH peak to the day of the LH peak. Cumulus echoes were observed in all follicles with a maximum diameter of more than 17 mm and the cumulus appeared from 1 to 3 days before ovulation. On the day after the LH peak, the follicle was still present in two cycles and had disappeared from 15 cycles. In 11 cases different morphologic patterns could be visualized at the former sites of the follicles. In the middle of the luteal phase the mature corpus luteum was seen as an echogenic area in 16 cases and as a cyst in 1 case.

Corpus Luteum↗
PubMed1985

Ectopic pregnancy and spontaneous abortions following in-vitro fertilization and embryo transfer.

In-vitro fertilization and embryo transfer was performed in 25 patients with diseased oviducts. Three pregnancies were achieved, but all ended in early abortion. One patient had ectopic pregnancy, one spontaneous abortion having a severe autosomal chromosome abnormality, and one had a rise in serum hCG but vaginal bleeding started 18 days after embryo transfer. We conclude that repeated ultrasonic examinations of all pregnancies following in-vitro fertilization and embryo transfer is recommendable until intra-uterine implantation has been confirmed and that amniocentesis should be offered to all these patients.

Abortion, Spontaneous↗
PubMed1985

Percutaneous oocyte recovery using ultrasound.

A technique for oocyte collection by percutaneous puncture guided by ultrasound is described. The equipment includes a sector scanner with a puncture transducer. A coarse needle (16 gauge) and a syringe constitute the aspiration device. The syringe provides the vacuum and serves as a trap for the follicular fluids. Follicular flushing is performed by syringes containing culture medium. The aspiration is carried out as an out-patient procedure under local anaesthesia after premedication. The spontaneously filled bladder acts as an ultrasonic window and the needle is introduced through the bladder into the ovarian follicles, guided by the screen display. The oocyte recovery rate per follicle exceeds 80% with experience, and the collection can be performed in women with abundant pelvic adhesions. The only complication to the procedure is a short-lasting haematuria, observed in 5% of the cases.

Anesthesia, Local↗