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Biomedical subjects

S Langer

Publications and source records attributed to S Langer.

At least 73 records · Page 4Linked to original sources
PubMed1996

[Arterioportal fistulas in the extra-hepatic circulation of the common hepatic artery. Diagnosis and radiologic interventional therapy].

Two cases of arterioportal fistulas and new possibilities in diagnosis and treatment are reported: A 62-year-old women was admitted to our hospital with abdominal pain. Color flow duplex sonography showed an arterioportal fistula between the proper hepatic artery and the ectatic portal vein that was confirmed by angiography, permitting percutaneous embolization as a definitive treatment. The pain disappeared immediately. A 36-year-old man underwent surgery due to a penetrating gastric ulcer. He was referred to us with an abdominal murmur. Color flow duplex sonography proved by spiral CT with 3D reconstruction and maximum intensity projection (angio-CT) revealed an arteriovenous fistula between the gastroduodenal artery and a mesenteric branch. Angiography confirmed the diagnosis and permitted definitive therapeutic embolization.

Adult↗
PubMed1996

The effects of excessive amounts of protein on lysine utilization in growing pigs.

Two experiments were conducted to investigate whether the utilization of lysine in growing pigs is affected by the level of excess protein in the diet. Nine lysine-deficient diets containing 100, 200 or 300 g crude protein/kg and between 1.2 and 6.8 g ileal digestible lysine/kg were prepared. In the first experiment the apparent ileal digestibility of lysine in three of the nine diets was determined using pigs with simple T-cannulas and Cr2O3 as an indigestible marker. Ileal digestibility of lysine in the other diets was calculated by interpolation. In the second experiment N retention, as a measure of lysine utilization, was determined in all nine diets using growing pigs over the weight range 30-50 kg. The effect of excess protein on lysine utilization was assessed by comparing the regression of N retention v. lysine (ileal digestible) intake at the three levels of protein. Increasing ileal digestible lysine in the diets resulted in a linear increase in N retention with all three protein levels and there was no significant difference amongst the three regressions, indicating that lysine utilization was not affected by the level of protein. Therefore, all data were pooled together to calculate a single regression for all treatments. An increase of 1.0 g ileal digestible lysine led to an increase of 1.43 g N or 8.96 g protein (N x 6.25) retained. Assuming a lysine concentration in the retained body protein of 65-72 mg/g, lysine was utilized with an efficiency of 0.58-0.65.

Animals↗
PubMed1995

Efficient selection of recombinant adenoviruses by vectors that express beta-galactosidase.

Adenovirus serotype 5 vectors which contain the Excherichia coli beta-galactosidase gene driven by the cytomegalovirus immediate-early promoter as a screenable marker have been made and successfully used in the construction of recombinant adenoviruses. The beta-galactosidase gene has been introduced into viruses in which the E3 region is maintained or deleted and in which the cis-acting packaging sequence has been reiterated at the right end of the chromosome. A unique BstBI site has been introduced 3' of the beta-galactosidase gene. Cotransfection of BstBI-digested vector DNA and a plasmid containing the left end of the viral chromosome followed by staining with X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) results in clear plaques when overlap recombination has occurred and blue plaques when ligation of the viral arms has occurred within the host cell. The beta-galactosidase-expressing viruses grow to lower titers than do the parental viruses, leading to a relative growth advantage for viruses resulting from overlap recombination. Combined with color selection based on the beta-galactosidase gene, this system permits efficient production and selection of recombinant viruses after cotransfection of BstBI-digested viral DNA with a plasmid including left-end viral sequences and the gene of interest. The beta-galactosidase-expressing viral DNAs were used to construct viruses containing BstBI sites on either side of the cis-acting packaging element as a means of testing their utility.

Adenoviridae↗
PubMed1992

Friend murine leukaemia virus is integrated at a common site in most primary spleen tumours of erythroleukaemic animals.

A common proviral integration site was identified and characterized in erythroleukaemias induced by Friend murine leukaemia virus (F-MuLV). Using inverse polymerase chain reaction, we found a proviral integration site common to at least 90% of 20 primary tumours tested. This site was identical to Fli-1, a locus recently reported by others to be rearranged in 75% (9/12) of cell lines established from spleens of erythroleukaemic mice and to code for a member of the ets gene family. Our data suggest that about half of the F-MuLV-induced erythroleukaemias contained more than one cell clone with a proviral integration in Fli-1, with different individual integration sites within Fli-1 in each cell clone. All proviruses were found to be integrated in the same transcriptional orientation with respect to flanking cellular DNA. We discuss these findings in relation to multistage models of neoplasm.

Animals↗
PubMed1985

Long-term results after incisional hernia repair.

After repair of incisional hernia, 154 patients were followed up for periods ranging from 4 to 10 years. An actuarial analysis showed a cumulated recurrence rate of 31%, with most recurrences appearing in the first three years. The recurrence rate after repair of recurrent incisional hernia was 44%. A cautious attitude to surgical treatment of incisional hernia is suggested if the patient has only mild discomfort.

Adolescent↗
PubMed1982

[Causes of anastomotic dehiscence].

The healing of intestinal anastomoses has many physiological, biochemical and morphological aspects. In each stage it can be disturbed or blocked. Nearly each factor of normal healing can under changed circumstances, favour anastomotic dehiscence. Dehiscence can be caused by one severe disturbing factor; in most cases, however, there is more than one factor that forms rupture of the anastomosis. The intestine itself can become a disturbing factor as well as changed biological circumstances and last but not least insufficient tactics and techniques of building an anastomosis.

Humans↗
PubMed1982

[Primary small intestine malignancies].

The frequency of malign tumors in the small intestine was 1% of all malign tumors of the digestive tract. 4% of malign tumors found by autopsy in the digestive tract were located in the small intestine. 12 months passed between first symptoms and operative therapy. In 80% we found local or distant metastases. In only 5 of 11 cases a curative resection was attempted. A great number pf small intestine tumors were identified by autopsy for the first time. A curative resection depends on an early diagnosis. Ileus, massive bleeding, extreme weight loss and palpable mass are late physical findings. Obstipation, diarrhea, occult intestinal bleeding and abdominal pain indicate after subtle inquiry X-ray of the small intestine, gastroduodenoscopy and angiography. If there is no result an early laparotomy should be performed.

Adenocarcinoma↗
PubMed1981

[Cryogenic surgery].

Local tumor destruction by cryosurgery was observed clinically and morphologically in 184 patients. If indication is observed strictly this method seems to provide real palliative effect. Complete tumor destruction was observed in 14.1%. Local tumor destruction was followed by tissue inflammation and nonspecific phagocytosis of tumor cells. Immunological reaction and epithelialization was noted only after radical destruction of tumors. Most cases showed immediate relief from stenosing cancer; colostomy could be avoided.

Cryosurgery↗
PubMed1981

[Cryotherapy in rectal cancer (author's transl)].

From 1972 to 1979 151 carcinomas of the rectum were treated by cryotherapy. Radical tumour destruction was observed in 19 cases. The other cases showed good palliation after multiple cryotherapy. In 48 biopsies the local effect of cryotherapy has been studied by SEM. Local tumour destruction was followed by tissue inflammation and nonspecific phagocytosis of tumour cells. Immunological reaction and epithelialization was noted only after radical destruction of the tumour.

Adenocarcinoma↗
PubMed1979

[Cancer of the colon: clinical and morphological aspects of local cryosurgery (author's transl)].

Using strict indications, local cryosurgery was performed in 86 patients with cancer of the colon. Survival time was longer both for patients with advanced tumour growth and distant metastases and those with potentially curable tumour but in too poor a general condition for operation. Other advantages of cryosurgery are the low complication and mortality rates, the possibility of out-patient treatment without anaesthesia, and thus a general reduction in cost. Indications for colostomy as final palliation can therefore be more narrowly defined than previously customary.

Aged↗
PubMed1978

[Colon anastomosis: single-layer (row) suture (author's transl)].

Comparison of two groups of patients with different anastomosis techniques (622 large-bowel resection) demonstrates a lower rate of complications with the single-row anastomosis. These observations were proved in dogs and rats. The double-row suture prolonged the healing process, delayed revascularization, and led to the formation of intramural abscess and stenotic intraluminal roll building. These problems were not observed in single-row anastomosis, which is designed to promote faster healing.

Abscess↗
PubMed1978

[Cryosurgical treatment of rectal carcinoma (author's transl)].

After comprehensive experimental especially morphological studies in animals, we started cryo-therapy of rectal carcinoma in 1972. We now present the first critical 5-years review concerning indication, clinical results and cryo-immunological features. In strongly indicated cases local tumour destruction by extremely low temperatures is superior to other palliative methods. Of 67 patients with rectal carcinoma treated with cryosurgery, 37 survived, 12 more than 36 months and 16 more than 24 months. Though there are only few hints indicating an increased immunological response following cryo-surgery, our investigations suggest a general influence on host's immunity by cryo-therapy.

Cryosurgery↗