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Biomedical subjects

S Kurimoto

Publications and source records attributed to S Kurimoto.

At least 73 records · Page 4Linked to original sources
PubMed1988

Adrenergic receptors in bovine retinal microvessels: presence of alpha 2- and beta- but not alpha 1-receptors.

In bovine retinal microvessels, alpha 1, alpha 2- and beta-adrenergic receptors were characterized by binding assay, using [3H]prazosin, [3H]para-aminoclonidine and [125I]iodocyanopindolol as radioligands, respectively. The microvessels were purified from bovine eyes by differential centrifugation through a high concentration of bovine serum albumin followed by use of a glass bead filtration technique. In the preparation, specific binding sites for [3H]para-aminoclonidine and [125I]iodocyanopindolol were observed, whereas [3H]prazosin binding was not detected. The [3H]para-aminoclonidine binding sites localized to the microvessels were characterized by high affinity and saturability (KD: 173 +/- 9 pM; Bmax: 394 +/- 11 fmol/mg protein) as well as the [125I]iodocyanopindolol binding sites (KD: 20 +/- 3 pM; Bmax: 43 +/- 4 fmol/mg protein). Furthermore, the specificity of both binding sites was pharmacologically evaluated by measuring the inhibitory effects of various adrenergic reagents on binding. The existence of alpha 2- and beta-adrenergic receptors which were characterized by high affinity, saturability and stereospecificity, leads to the hypothesis that the retinal microcirculation is under neuronal control.

Animals↗
PubMed1987

Effect of methylcobalamin in accommodative dysfunction of eye by visual load.

The effect of methylcobalamin on the accommodative function of subjects with deteriorated accommodation by induced experimental visual work was studied by measuring small fluctuations of accommodation. The deterioration of small fluctuations of accommodations were difficult to find after visual work in the group receiving methylcobalamin. However, a significant difference of small fluctuations of accommodation was recognized in the non-administered group and in the placebo group (P less than 0.05), and that change was higher in the non-administered group than in the placebo group. Although, the placebo effect of methylcobalamin was undeniable, it was objectively confirmed that methylcobalamin produced a good effect on deteriorated accommodation induced by the visual work.

Accommodation, Ocular↗
PubMed1987

Evaluation of vergent eye movement using the jumping method.

The state of vergent eye movement is reported before and after the experimental work. The work performed by 26 subjects was a name search task on a visual display terminal (VDT). Convergence and divergence eye movements were recorded objectively using an accommodopolyrecorder, and as a sensor of eye movement, elictro-oculogram (EOG) was applied. These eye movements were elicited by watching each target, a far fixation target and a near fixation target, which alternated from far to near every five seconds on the accommodopolyrecorder. Measurements by this method were conducted at two points in time: before beginning the VDT work and after completing the work. The convergence time was unchanged before and after the work load. The divergence time, however, was prolonged after the work load, which was highly significant (P less than 0.001). In this study, it is shown that the vergent eye movement, convergent and divergent eye movement, is influenced by VDT work.

Accommodation, Ocular↗
PubMed1987

Electron microscopic evidence for epidermal growth factor receptor (EGF-R)-like immunoreactivity associated with the basolateral surface of gastric parietal cells.

Epidermal growth factor (EGF), in addition to its effects on cell growth, has a suppressive effect on gastric hydrochloride secretion. We recently demonstrated EGF-receptor (EGF-R)-like immunoreactivity on human gastric parietal cells by light microscopy. To reveal further the localization of this reaction an immuno-electron microscopical study was performed. 528IgG, an anti-EGF-R murine monoclonal antibody and a polyclonal anti-EGF antibody were employed for immunostaining using the avidin-biotin method. Positive reaction against 528IgG was shown mostly on the outer membrane of parietal cells, except in the apical portion. No reaction was observed on most of the intracytoplasmic membranes including intracellular canaliculi, endoplasmic reticulum and other membranous components. In the basillar portion, the 528IgG-positive parietal cell membrane formed labyrinthine interdigitations. No reaction against anti-EGF was demonstrated in any of the gastric mucosal cells, although a reaction was clearly shown on salivary gland cells. These findings suggest a blood-mediated direct action of EGF on parietal cells.

Cell Membrane↗
PubMed1986

[The working adaptation in visual display terminal (VDT) workers].

The working adaptation in VDT workers was conducted by investigating from two different standpoints: one was to indicate an improvement of working conditions and environments around the VDT workers, evaluating the fundamental data of their visual physiological functions, and the other was to investigate the relation between their performances and production. The results were as followed: 1. Eye strain induced by VDT work was able to take an objective view as accommodative and convergent depression including the tear productive function, followed by making good working conditions. 2. The measurement of eye movement was able to analyze the response time and the response process for the informations indicating on the display, and the concept of recovery time for deteriorated accommodative function was useful to improve the display design. 3. It was suggested that the measurement of visual evoked potential was more simple method to evaluate the workers' attention. 4. The relation between performance and psychological stress and the time course analysis of performance were studied, so that it was given suggestions as to assign suitably the work management and the working design to each VDT worker.

Accommodation, Ocular↗
PubMed1985

Surface markers of peripheral blood lymphocytes in Vogt-Koyanagi-Harada disease.

The cell membrane markers on peripheral blood lymphocytes of 10 patients with Vogt-Koyanagi-Harada disease (VKH) were analyzed by laser flow cytometry using monoclonal antibodies. Seven patients with active uveitis and 98 normal control subjects were also studied. The percentage of OKT3+, OKT4+ and OKT11+ cells were significantly lower while OKIa1+ cell were higher in VKH compared to controls. The percentage of cells reacting with monoclonal antibodies were slightly affected during steroid therapy, but returned to the pretreatment level after cessation of the therapy. OKIa1+ cells were shown to be increased in active uveitis while percentages of cells with other cell markers remained unchanged when the results were compared with that of the control. The findings suggest that the analysis of cell membrane markers on lymphocytes in VKH could be a significant parameter in clinical medicine.

Adult↗
PubMed1984

Active T rosette formation of human T lymphocytes after stimulation with lectins.

The effect of fourteen plant lectins on human peripheral blood lymphocytes in rosette formation with sheep red blood cells (SRBC) was examined. Among the lectins used, phytohemagglutinin (PHA), lens culinalis (LCH), wheat germ agglutinin (WGA), and ricinus communis I (RCAI) showed a significant increase in active T rosette counts when the results were compared with the effect of other lectins. This enhancement in active T rosette forming cells is found within one hour after the treatment with lectin and is dependent upon the concentration of lectin. The results suggest that the changes in lymphocyte membrane dynamics via stimulation by certain lectins increases the capacity to form active T rosettes.

Animals↗