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Biomedical subjects

S Kurihara

Publications and source records attributed to S Kurihara.

At least 163 records · Page 9Linked to original sources
PubMed1984

[Biochemical studies on alkaline phosphatase isoenzyme profile in endometrial cancer].

Heat-stable ALP has been attracting attention as one of the oncodevelopmental proteins. ALP are now subdivided by biochemical methods into three major groups e.g. tissue-unspecific, intestinal and term placental isoenzymes. Furthermore, term placental ALP in cancer is classified into L-leucine insensitive-Regan and L-leucine sensitive-Nagao isoenzymes. In this paper, we report ALP isoenzyme profiles of normal endometrium and endometrial cancers. 21 cases of normal endometrium and 11 cases of endometrial cancer were examined, and the ALP activity of their butanol extracts was measured by Fishman's method. To identify the ALP isoenzyme pattern, biochemical methods such as 65 degrees C heat-treatment and amino acid inhibition tests were carried out. Most of the ALP in both normal endometrium and endometrial cancers showed biochemical characteristics of tissue-unspecific ALP isoenzyme. A very small amount of term placental isoenzyme was found in normal endometrium. In endometrial cancers, term placental isoenzyme was often found at a higher level than in normal endometrium. Nagao isoenzyme was also found to emerge in a few endometrial cancer cases. From these data it was suggested that the ALP isoenzyme of endometrium underwent a change and "enzyme deviation" occurred during the course of endometrial carcinogenesis and that the ALP isoenzyme deviation might have occurred due to a change in ALP isoenzyme gene expression accompanying cancerous change.

Alkaline Phosphatase↗
PubMed1984

[Peplomycin sensitivity of various types of human gynecological cultured tumor cells].

Various types of human gynecological cultured tumor cells were tested for the sensitivity to Peplomycin (PEP), an effective antitumor antibiotic for squamous cell carcinomas, by the regrowth assay method together with morphological observation. Bleomycin-hydrolase activity of these cell lines was also compared in cell-free extracts by assaying the conversion of Bleomycin into its deamidated from (HPLC method). SKG-I, SKG-II, SKG-IIIb cells derived from squamous cell carcinoma of the cervix and RKN cells derived from myosarcoma of the ovary were much more sensitive to PEP than other cell lines. PEP was found to be mainly a time-dependent drug, but also concentration dependent. The effect of PEP on cell morphology was characterized by the appearance of enlarged cells and swelling nuclei. The specific activities of Bleomycin-hydrolase in SKG-I, SKG-II, SKG-IIIb cells were shown to be relatively lower than that in other cell lines. These results suggested that cervical squamous carcinoma cells and ovarian myosarcoma cells were sensitive to PEP and Bleomycin-hydrolase activity was one of factors which decided the PEP sensitivity of human cultured tumor cells.

Antibiotics, Antineoplastic↗
PubMed1984

[Shift of placental alkaline phosphatase isoenzymes in the course of pregnancy].

It is generally accepted that every placental protein shows its characteristics vicissitude pattern during pregnancy and that alkaline phosphatases (ALP) are subdivided into three kinds of isoenzymes, i.e.: tissue-unspecific, intestinal and termplacental ALPs. In this study, the changes in ALP isoenzyme profile throughout the course of pregnancy was studied by biochemistry, enzyme- and immuno-histochemistry, and the following results were obtained. In early pregnancy, ALP activity was composed mostly of tissue-unspecific ALP isoenzyme, and its activity revealed a peak value around 10 weeks of pregnancy as in the case of the hCG serum concentration. At the end of the second trimester of pregnancy, instead of tissue-unspecific isoenzyme, ALP activity was mainly composed of term placental ALP isoenzyme, but a very small amount of the isoenzyme was proved to exist in the villi at the 6th week of gestation. The above results were confirmed by morphological studies such as enzyme-cytochemistry and immuno-cytochemistry. It was therefore concluded that at least two genes of ALP isoenzymes existed in the villi and that the regulation of gene activity changed during the course of pregnancy. Furthermore, since tissue-unspecific ALP isoenzyme showed a pattern of change similar to that of serum hCG, the existence of a developmental phase-specific gene set was postulated.

Alkaline Phosphatase↗
PubMed1984

[A newly established melanoma cell line (GAK) with 5-S-cysteinyldopa phenotype].

The production of 5-S-cysteinyldopa by a newly established melanoma cell line GAK is reported. The cell line was derived from a metastatic inguinal lymph node of vulvar malignant melanoma. The cell line grew well without interruption for over 4 years, GAK cells were proved to have melanin granules and tyrosinase activity in their cytoplasma by Masson's staining and dopa reaction, respectively. Melanin granules were ultrastructually identified as melanosomes in various maturing stages. The chromosomal number varied widely and showed aneuploidly, but the modal chromosomal number was stable in the hypotriploid range. GAK cells were transplanted to nude mice and produced tumors resembling the original. Because glucose-6-phosphate dehydrogenase of GAK revealed a type B (slow) mobility pattern on electrophoresis, the possibility of Hela cell contamination could be completely excluded. High performance liquid chromatography revealed "5-S-cysteinyldopa", a new tumor marker of malignant melanoma, in culture media of GAK cells. The cell line described may serve as a representative model system for basic and clinical studies on malignant melanoma.

Aged↗
PubMed1984

[Correlation between cell cycle and cell morphology of RKN cells].

UNLABELLED: In order to study the changes in the morphology of each phase of the cell cycle, RKN cells, derived from a human ovarian myosarcoma, were synchronized by a mitotic selection method and studied under time-lapse cinematography, light microscopy, scanning and transmission electron microscopy. RESULTS: M-phase: The cells with relatively a few cytoplasmic organelles were small and spherical, and chromosome were seen in them. The cell surface was covered with numerous mivilli. Late G1,S and early G2 phase: The large, thin cells were flat and polygonal with various organelles inside and had central nuclei. Most of the chromatin patterns were finely granular. No microvilli were found on the cell surface. Early G1-phase: The paired daughter cells were fanshaped and the nuclei were eccentric with finely granular chromatin patterns. At the edges of the cells, rufflers which were related to cell movements were often seen. Late G2-phase: The thick cells were of a spindle shape and the chromatin patterns were a coarse granular type. Again on the cell surface microvilli increased and the slender filopodia were often seen.

Cell Cycle↗
PubMed1984

[Modulation of alkaline phosphatase by butyrate and prednisolone in uterine cervical cancer cell line (SKG-III)].

The production of late placental alkaline phosphatase (ALP) isoenzyme and the co-presence of a small amount of tissue-unspecific ALP isoenzyme was confirmed in the newly established uterine cervical epidermoid cancer cell line SKG-III. Sodium butyrate (3mM), which has been shown to modulate oncodevelopmental gene expression, was examined after introduction of the agents into confluent cultures. 72 hours after the first sodium butyrate treatment, the amount (per mg cell protein) of total ALP activity increased to 5 times that of control cells and tissue-unspecific insoenzyme occupied about two thirds of the concentration of the modulated ALP. Treatment of SKG-III cells with prednisolone (5 micrograms/ml) for 48 hours caused a 3.5 times increase in total ALP activity, and most of the modulated ALP was occupied by late placental isoenzyme. From these data, it was concluded that sodium butyrate mainly induced tissue-unspecific isoenzyme, while prednisolone induced late placental isoenzyme. The results suggested that SKG-III cells had at least two ALP isoenzyme genes and chemical agents such as sodium butyrate or prednisolone could modulate the ALP isoenzyme profile of these cultured cells in vitro.

Alkaline Phosphatase↗
PubMed1984

[Biological properties of two newly established cell lines (SKG-3a,3b) from a human uterine cervical epidermoid carcinoma].

This is an initial descriptive report of two morphologically different cell lines (SKG-3a and SKG-3b) obtained from a human uterine cervical epidermoid carcinoma. These sublines were found to be derived from a moderately differentiated epidermoid carcinoma partially mixed with clear cell components. Both sublines had already been subcultivated more than 80 times since the initial separation. SKG-3a cells were much smaller in size and volume than 3b. The numbers of chromosomes were almost identical. Their cytology revealed anaplastic and pleomorphic features. SKG-3a was positive with periodic acid-Schiff stain and this changed to negative after an amylase digestive test, suggesting that the cells contained glycogen. An electron-microscopic examination confirmed the presence of many glycogen particles in 3a cytoplasm, but few in 3b. Tonofilaments and desmosomes were observed in both cytoplasms, suggesting epidermoid origin. In nude mice, SKG-3a produced clear cell epidermoid carcinoma with much glycogen, while 3b grew as a moderately differentiated epidermoid carcinoma with little glycogen. It is concluded that SKG-3a is derived from the clear cell epidermoid carcinoma and 3b is from the moderately differentiated epidermoid carcinoma. It is also clear that both were more heat sensitive at and over 39 degrees C than normal cells.

Adult↗
PubMed1983

Sodium butyrate produces concordant expression of "early placental" alkaline phosphatase, pregnancy-specific beta 1-glycoprotein and human chronic gonadotropin beta-subunit in a newly established uterine cervical cancer cell line (SKG-IIIa).

The production of early and term placental alkaline phosphatase (ALP), human chorionic gonadotropin beta-subunit (beta-hCG) and pregnancy-specific beta 1-glycoprotein (SP1) was confirmed in the newly established uterine cervical cancer call line SKG-IIIa. Treatment of these cells in culture with sodium butyrate caused an increase of all of these oncodevelopmental proteins. On the other hand, prednisolone treatment enhanced only term placental ALP, while it reduced early placental ALP, beta-hCG and SP1. These data suggested that such oncotrophoblastic proteins as early placental ALP, beta-hCG and SP1 were concordantly modulated by butyrate and prednisolone. These findings may support the possibility of the reexpression of sets of development phase-specific genes in cancer cells.

Alkaline Phosphatase↗