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Biomedical subjects

S Kuno

Publications and source records attributed to S Kuno.

At least 163 records · Page 9Linked to original sources
PubMed1979

Circulating immune complexes in the serum of diabetes mellitus in childhood by a modified 125I-C1q binding test.

The 125I-C1q binding test for the detection of soluble immune complexes in native unheated human serum was applied to the study of sera from 52 patients with diabetes mellitus in childhood. This radiolabeled C1q binding test is more sensitive and reproducible among the various methods proposed for the detection of immune complexes. The 125I-C1q binding activity in 52 sera from diabetes mellitus in childhood was 9.47 +/- 0.36% compared to 6.94 +/- 0.74% in normal controls. 125I-C1q binding values in diabetes mellitus in childhood were significantly higher than normal controls. Slight high values were seen in 3 patients with positive anti-DNA-antibodies in diabetes mellitus in childhood. 125I-C1q binding was not significantly increased in patients with positive antithyroid antibodies and insulin antibodies. There was no significant correlation between the duration of diabetes and 125I-C1q binding activity.

Adolescent↗
PubMed1979

Antimicrosomal antibodies, gastric parietal cell antibodies and antinuclear factors in insulin dependent diabetes mellitus.

Thyroid antimicrosomal antibodies, gastric parietal cell antibodies (PCA) and antinuclear factors were studied in 208 insulin dependent diabetic (IDD) according to the duration of diabetes and patient's age at the time of testing. Antimicrosomal antibodies were found in 11 out of 47 (23.4%) IDD with the duration of less than one year, however this value declined to 13.1% at 1 to 3 years, 15.3% at 4 to 5 years, 10.8% at 6 to 10 years and 5.8% at more than 10 years. Of the 47 IDD, 7 (14.8%) were positive for gastric parietal cell antibodies. The prevalence of PCA declined with increasing duration of diabetes. However, this decrease in the prevalence of antimicrosomal antibodies and PCA was not so extreme as that of pancreatic islet cell antibodies. Antinuclear factors did not reveal a significant correlation with the duration of diabetes. In normal controls, the prevalence of antimicrosomal antibodies, PCA and the antinuclear factors increased progressively with age. In IDD, the prevalence of the antinuclear factors was also progressively greater with age. However, the prevalence of antimicrosomal antibodies in IDD decreased with age and those of PCA showed the lowest percent in the 40-69 year-age group.

Adolescent↗
PubMed1979

The leucocyte migration inhibition test and subpopulations of peripheral lymphocytes in insulin-dependent diabetics.

The leucocyte migration inhibition test (LMT) was performed by the agarose plate method with thyroid and pancreatic antigens in patients with insulin-dependent or independent diabetes mellitus. The mean migration indices with thyroglobulin, thyroid mitochondria and beef insulin were not significantly different in insulin-dependent diabetics from those in insulin-independent diabetics or normal controls. However, significant inhibition of leucocyte migration was observed in insulin-dependent diabetics when thyroid microsome or pancreatic extract was used as antigen. Although no significant difference was found in the percentages of T and B lymphocytes between insulin-dependent diabetics and insulin-independent diabetics or normal controls, the results of LMT strongly suggest the presence of cellular immunity against the thyroid and pancreas in insulin-dependent juvenile-onset diabetes.

Adult↗
PubMed1978

[Subpopulations of peripheral lymphocytes in insulin-dependent diabetics (author's transl)].

There is an increasing evidence that autoimmune mechanisms may have a role in the pathogenesis in insulin-dependent diabetics. The numerical and functional study of peripheral blood lymphocytes in diabetes mellitus might indirectly contribute to the understanding of its pathogenesis. In this study, detection of peripheral blood T lymphocytes was measured by rosettes with sheep red blood cells (SRBC), and B lymphocytes were measured by immunofluorescence with specific antiserum to immunoglobulins. The mean (+/- SD) percentage of SRBC was 67.6 +/- 7.2 in 21 normal subjects, 71.5 +/- 7.0 in 15 insulin-dependent diabetics, and 68.6 +/- 6.7 in 30 insulin-independent diabetics. There was no difference in the absolute T-lymphocyte number per mm3 in these three groups. Insulin-dependent diabetics showed a normal percentage and absolute number of B lymphocytes when compared with normal subjects.

Adult↗
PubMed1977

[Leucocyte migration inhibition test in diabetes mellitus (author's transl)].

The leucocyte migration inhibition test (LMT) using the agarose plate method introduced by Clausen is simple and highly reproducible. Using human pancreas extract and beef insulin as antigen, LMT was performed on ten patients with insulin dependent diabetes, twenty patients with insulin independent diabetes, and twelve healthy controls. The migration index was expressed as a percentage of migration calculated from the following formula. Migration index (MI) = average areas of migration in test suspension/average areas of migration in control suspension. Using human pancreas extract, the mean migration index for the insulin dependent diabetics (87.6 +/- 11.1) was significantly lower than in the normal subjects (99.3 +/- 6.3) (p less than 0.05). Using beef insulin as antigen for the insulin dependent diabetics and insulin independent diabetics, the mean migration indices (+/- SD) were 95.8 +/- 14.9 and 98.7 +/- 12.3 respectively. The corresponding values for the control group were 98.9 +/- 7.8. Cellular hypersensitivity to human pancreas extract was shown in the leucocyte migration inhibition test with insulin dependent diabetics, but a negative result was obtained with beef insulin.

Adult↗
PubMed1977

[Leucocyte migration inhibition test in diabetes mellitus (author's transl)].

The leucocyte migration inhibition test (LMT) using the agarose plate method introduced by Clausen is simple and highly reproducible. Using thyroglobulin, mitochondria and thyroid microsomal fractions as antigens, LMT was performed on ten patients with insulin dependent diabetics, twenty patients with insulin independent diabetics, eleven patients with Graves' diseases, ten patients with chronic thyroiditis and ten healthy controls. The migration index was expressed as a percentage of migration and calculated from the following formula; (formula: see text). Using a thyroid microsomal fraction, the mean migration index for the insulin dependent diabetics (93.2 +/- 11.6) was significantly lower than in the normal subjects (104.3 +/- 10.9) (0.025 less than p less than 0.05). Using thyroglobulin as an antigen for the insulin dependent diabetics and insulin independent diabetics, the mean migration indices (+/-SD) were 93.7+/-10.8 and 102.8+/-10.6 respectively. The corresponding values for the control group were 100.6+/-7.3. Using a mitochondria, MI values for insulin dependent and independent diabetics were 97.7+/-5.5 and 99.6+/-12.6 respectively, while MI value for the control group was 103.9+/-13.6. The mean migration indices obtained with the mitochondria and thyroglobulin were not significantly depressed when the diabetic groups were compared with the normal subjects.

Cell Migration Inhibition↗
PubMed1977

Macromolecule synthesis of Escherichia coli BB at a lower or transient growth state.

Macromolecule synthesis in Escherichia coli BB at lower growth rates was investigated. The results indicate that a deviation in ribonucleic acid (RNA) content per cell at a lower growth rate from the exponential relationship to a specific growth rate is entirely attributable to the presence of nonviable cells, in which the RNA content is lower than in viable cells. Based on this fact, a mathematical expression of macromolecule contents versus specific growth rate was devised. Moreover, continuous changes in macromolecule content during unbalanced growth from late-logarithmic phase to stationary phase were measured. Although growth rates changed continuously, the data on deoxyribonucleic acid (DNA) or RNA content versus the specific growth rate calculated from the increments in cell number satisfactorily fitted the exponential lines obtained under balanced growth at a higher growth rate. However, no such relationship was observed in the plot of DNA or RNA content versus the specific growth rate calculated from the increments in optical density.

Bacterial Proteins↗
PubMed1977

Clinical significance of fucose level in glycoprotein fraction of serum in patients with malignant tumors.

Serum fucose content in the glycoprotein fraction was determined in various patients with malignant and benign diseases. The results showed that, in contrast to benign diseases, malignant diseases were characterized by an increased fucose content in the glycoprotein fraction. However, no significant difference was noted in the fucose levels in the mucoprotein fraction. The increased fucose level in glycoprotein in malignant diseases was parallel to the increment in total fucose content in serum, which suggests that the increased levels in total fucose in malignant diseases, reported previously, are primarily due to the increase in fucose-containing glycoprotein.

Female↗
PubMed1976

[Thyroglobulin and microsomal antibodies in diabetes mellitus in childhood (author's transl)].

The purpose of our study was to determine the incidence of thyroid antibodies in diabetes mellitus in childhood and to discuss the correlation between thyroid antibodies and insulin antibodies. The sera of 50 children with diabetes mellitus and 437 children as disease controls were tested by thyroglobulin and microsome-coated tanned red cell hemagglutination test (Fuji Zoki Co. Tokyo). One of the 50 children with diabetes mellitus (2%) was positive with antithyroglobulin antibodies and eleven (22%) were positive with antimicrosomal antibodies compared to 0.4% and 1.1% respectively in 437 disease controls. To clarify the association of insulin antibodies and thyroid antibodies in diabetes mellitus in childhood, insulin antibodies were demonstrated by using a modified method described by Wright. One of the 33 children with negative insulin antibody (2.8%) was positive with antithyroglobulin antibodies and eight (24%) were positive antimicrosomal antibodies. No evident correlation was observed between antithyroid antibodies and insulin antibodies.

Adolescent↗
PubMed1976

Macromolecule synthesis in Escherichia coli BB under various growth conditions.

The kinetic behavior of the macromolecule synthesis of Escherichia coli during balanced growth in various media at different temperatures as investigated. The results indicate that macromolecule contents per cell can be expressed as exponential functions of the specific growth rate at a given temperature. It was shown that the content per cell at the zero growth rate was constant in each macromolecule component, irrespective of the growth temperature. The rate of ribonucleic acid (RNA) synthesis per unit weight of deoxyribonucleic acid and that of protein synthesis per unit weight of RNA were taken as efficiencies of RNA and protein synthesis, respectively; both of them were found to be dependent on the growth rate and temperature. The efficiency of RNA synthesis was found to be very high at a high growth rate, whereas that of protein synthesis was found to decrease above certain growth rate. At the same growth rate, an increase in the growth temperature resulted in a decrease in the efficiency of RNA synthesis but an increase in that of protein synthesis.

Amino Acids↗
PubMed1975

An inhibitory effect of thymidine on its own conversion to nucleotide in Escherichia coli.

The cellular levels of thymidine nucleotide pool in thymine-requiring mutants of Escherichia coli were followed. The pool levels of dTDP, dTTP and compound-X reached maximal values in much lower concentration of thymidine than those in thymine. In higher concentrations of thymidine, an inhibitory effect on its own conversion to nucleotide was observed. The inhibited step was suggested to be the conversion of dTMP to dTDP.

Escherichia coli↗
PubMed1975

Effects of growth conditions on thymidine nucleotide pools in Escherichia coli.

The cellular levels of thymidine nucleotide derived from [3H]thymine or [3H]thymidine were followed under various environmental conditions with a thymine-requiring mutant of Escherichia coli. It was shown that the pool sizes varied greatly with the growth conditions; that is, with growth temperature, inhibition of DNA synthesis of replacement of thymine with thymidine. In the strain used here, the level of compound X, presumably dTDP-sugar, was very much higher than those of other thymidine nucleotides. It is suggested that the conversion of thymine to thymidine is rate-limiting, while the conversions of thymidine to dTMP, and of dTMP to dTDP are more rapid than other steps in the salvage pathway of thymidine nucleotide.

Cytarabine↗