Biomedical subjects

S Kunkel

Publications and source records attributed to S Kunkel.

At least 55 records · Page 3Linked to original sources

PubMed1987

[Results of the GnRH-TRH and arginine-GnRH-TRH test in females with hyperprolactinemic galactorrhea].

In 7 patients aged from 21 to 33 years (average age 24.6 years) with hyperprolactinemic galactorrhea a stimulation test with GnRH-TRH or arginine-GnRH-TRH was performed. The serum levels of LH, FSH, PRL, HGH, TSH and total thyroxine (T4) were determined by RIA, the thyroxine binding capacity (TBC) by radio agent assay. The free thyroxine index (FT4-I) was calculated. An adenoma of the pituitary gland was diagnosed in 3 patients by X-ray of sella turcica. In all patients there were disturbances of the menstrual cycle. Independent of the existence of an adenoma of the pituitary gland in all patients with high basal levels. PRL could not have been shown stimulated by TRH. On the other hand it could be shown that despite of high basal PRL-levels in serum there was a normal stimulation of the gonadotrophs in 5 patients. Out of one patient the stimulation of the thyreotropic cells of pituitary by TRH was normal, too, whereas there was no or an inadaquate stimulation of HGH by arginine in all patients.--T4, TBC and FT4-I showed no deviation from the normal range.

Adenoma↗

PubMed1987

[GnRH-TRH and arg-GnRH-TRH test in females with normoprolactinemic galactorrhea].

In 8 patients aged from 17 to 48 years (average age 32,6 years) with normoprolactinemic galactorrhea a stimulation test with GnRH-TRH or arginine-GnRH-TRH was performed. The basal and stimulated serum levels of LH, FSH, PRL, TSH, HGH and total thyroxine (T4), the thyroxine binding capacity (TBC) and the free thyroxine index (FT4-I) were determined. -In a few cases there were disturbances of both the basal and the stimulated serum levels of the hormones. -Both the menstrual disorders which were seen in all patients with galactorrhea and the hormonal disturbances are discussed in context of an hypothetical increase of the PRL receptor sensibility in face of normal PRL serum levels or as a hint at a transient hyperprolactinemia.

Adolescent↗

PubMed1987

[Hormone profile and follicle development in ovarian stimulation treatment for in vitro fertilization. 1. FSH and LH profiles].

144 patients were treated by 5 different stimulation protocols in the IVF-program of the University Women's Hospital Rostock (Clomiphene/HCG, Clomiphene/Anthrogon/HCG, Pergonal/HCG, Anthrogon/HCG, Folistiman/HCG). In 172 cycles FSH and LH were measured in serum during the follicular phase by RIA. The results were compared between the treatment groups and a group of normal women by Student's t-test or Mann-Whitney's nonparametric test. Moreover, in each group the number of follicles visible by laparoscopy and number of punctured follicles were estimated. The maturation of collected oocytes were judged by a maturation index. Modus of action and FSH/LH-ratio of used drugs have been shown to influence serum levels of FSH. Every stimulation protocol was characterized by typical profiles of hormones. Especially, immunoreactive levels of FSH were related to development of follicles. Different biological action of various stimulation regimes was demonstrated by a shortage of follicular phase from 13,4 days in the Clomiphene group to 11,0 days in the Folistiman group, an increase of laparoscopically visible follicles from 4,0 to 13,2 and a rise of collected oocytes from 1,6 to 7,3 per laparoscopy. Quality of oocytes has been shown to be related significantly to the modus of stimulation. Anthrogon-protocol was the most effective stimulation regime according to profile of gonadotropins, the number of follicles and the structure of harvested population of oocytes. However, even in this group co-dominant development of follicle was not achieved. Application of pure FSH and an earlier start of stimulation treatment are possibilities for improvements of results in IVF by stimulation.

Chorionic Gonadotropin↗

PubMed1986

Endocrine cells in adenocarcinomas and their prestages in the glandular stomach and duodenum of rats after MNNG administration. Histochemical, electron microscopical and radioimmunological studies.

Tumours of the glandular stomach and upper small intestine were induced in rats by oral administration of MNNG. In most cases the lesions were identified histologically as adenocarcinomas and their prestages, such as polypeous and downward growing adenomatous hyperplasias. Out of 48 adenomatous hyperplasias and adenocarcinomas of the stomach and 24 well differentiated adenocarcinomas of the small intestine, we observed argyrophilic cells in nearly the half of the cases. Endocrine cells were also identified by electron microscopy. The frequency of endocrine cells was reduced with decreasing degree of tissue differentiation. In poorly differentiated carcinomas, including signet ring cell carcinomas, no argyrophilic cells were found. Out of 10 adenomatous hyperplasias and tumours of the stomach investigated immunohistochemically, 5 cases showed gastrin producing cells. Most of these animals were radioimmunologically characterized by strongly elevated serum gastrin levels. Derivation and potential relevance of the endocrine cells in tumours are discussed.

Adenocarcinoma↗

PubMed1986

[Effect of mestranol and chlormadinone acetate on TSH, T4, TBC and FT4 index in Turner syndrome].

In 19 patients with Turner's syndrome aged from 12 to 24 years (average age 17.0 years) the influence of mestranol and chlormadinone acetate on both basal and TRH stimulated TSH secretion, total thyroxine (T4), thyroxine binding capacity (TBC) and free thyroxine index (FT4-I) by means of sequential stimulation test (0.5 g arginine hydrochloride/kg body weight, 25 micrograms GnRH and 200 micrograms TRH) was investigated. These investigations were performed before hormonal substitution, during third to 5th cycles of treatment and 4 month after finishing treatment. TSH and T4 serum levels were determined by RIA, TBC by radio reagent assay. The FT4 index was calculated. Mean basal TSH levels both before, during and after hormonal treatment did not differ. Netto TSH level increase were somewhat higher in 8 of the 19 patients during hormonal treatment. TBC and T4 significantly increased under treatment. The rise of FT4 in a few patients under treatment could not be ensured statistically. After finishing treatment the levels of thyroid parameters decreased.

Adolescent↗

PubMed1985

[Effect of mestranol and chlormadinone acetate on basal and GnRH stimulated gonadotropin secretion in Turner's syndrome].

In 19 patients aged from 12 to 24 years (average age 17.0 years) with Turner's syndrome the influence of mestranol and chlormadinone acetate on both basal and GnRH stimulated secretion of gonadotropins was investigated by means of sequential test (0.5 g arginine hydrochloride/kg body weight, 25 micrograms GnRH and 200 micrograms TRH). This test was performed before, during and after treatment. Serum levels of LH and FSH were determined by RIA. Before hormonal treatment 15 patients showed hypergonadotropic, and 4 normo- or hypogonadotropic basal levels, respectively. By GnRH the increase of the gonadotropin secretion was individually different. Under hormonal influence the basal gonadotropin levels were significantly reduced, with the responsibility to GnRH going lost. After treatment in 15 women hypergonadotropic levels were recovered. But FSH serum levels were somewhat lower and LH levels somewhat higher than before hormonal treatment.

Adolescent↗

PubMed1985

[Effect of mestranol and chlormadinone acetate on basal and TRH-stimulated PRL secretion in Turner's syndrome].

In 19 patients aged from 12 to 24 years (average age 17.0 years) with Turner's syndrome the influence of mestranol and chlormadinone acetate on both basal and TRH stimulated PRL secretion was investigated by means of sequential stimulation test (0.5 g arginine hydrochloride/kg body weight, 25 micrograms GnRH and 200 micrograms TRH). This test was performed before, during the third until 5th cycle of treatment and 4 month after the end of therapy. PRL levels were determined by RIA. In most patients under treatment both basal and stimulated PRL showed an increase within the normal range. Hyperprolactinemic levels could be observed on one patient only.

Adolescent↗

PubMed1985

[Effect of mestranol and chlormadinone acetate on HGH secretion in Turner's syndrome].

In 19 patients aged from 12 to 24 years (average age 17.0 years) with Turner's syndrome the influence of the hormonal substitution with mestranol and chlormadinone acetate on both basal and arginine stimulated HGH secretion was investigated. This test was performed before, during and after treatment. HGH serum levels were determined by RIA. Both the basal values and the increase of HGH secretion after arginine stimulation were used for evaluation of the test results. Under estrogen-gestagen treatment in 15 of the 19 patients hypersomatotropic basal levels were determined. The basal HGH levels before and during as well as during and after treatment are significant different (p less than 0,01). The responsibility of the somatotrophs to the arginine stimulation was individually different and did not show any uniform trend.

Adolescent↗

PubMed1985

[The coincidence of gonadal dysgenesis and hyperprolactinemia].

It was reported about 6 female patients aged from 3 to 31 years (average age 18 years) with gonadal dysgenesis and hyperprolactinemia. Beside one all these females were not treated with synthetic sexual steroids. In 5 of the 6 patients both hyperprolactinemic and hypergonadotropic serum levels were observed. In one case the gonadotropic serum levels were normal. In 4 patients a stimulation test with GRH-TRH or Arg-GRH-TRH, respectively, was performed. In patients with gonadal dysgenesis a change on the area of the hypothalamic-hypophyseal axis is supposed to cause the hyperprolactinemia.

Adolescent↗

PubMed1985

[Primary hyperprolactinemic amenorrhea. Report of 4 patients].

Report about 4 female patients with hyperprolactinemic primary amenorrhea. In two of these women there was a suspicion of adenoma of hypophysis. In the stimulation test with gonadotropin-releasing hormone and thyrotropin-releasing hormone in two females the very high basal PRL secretion could not have been stimulated in one case. In the other case the TRH-stimulated PRL serum levels increased. Biphasic menstrual cycles resulted by therapy in two females treated with bromoergocryptine.

Adenoma↗

PubMed1985

[Arginine-GnRH-TRH test in patients with primary hypothalamic amenorrhea].

In 8 patients with hypothalamic primary amenorrhea aged from 16 to 23 years (average age 19,4 years) a sequential stimulations test was performed with 0,5 g arginine hydrochloride per kg body weight, 25 micrograms gonadotropin-releasing hormone (GnRH) and 200 micrograms thyreotropin-releasing hormone (TRH). The response of the lactotropic, gonadotropic, thyreotropic and somatotropic cells of the pituitary was investigated. Serum levels of PRL, LH, FSH, TSH and HGH were determined by RIA. In all women hypoplastic ovaries were found by laparoscopy. In 7 patients tissue biopsies showed primordial follicles or primordial and secondary follicles, respectively. Investigations point to, that in hypothalamic primary amenorrhea at first the function of the gonadotropic and lactotropic cells of the pituitary is injured. The somatotropic cells could not be stimulated in 3 of 8 patients, the function of hypothalamo-pituitary-thyroid-axis in the stimulations test was normal in all women.

Adolescent↗

PubMed1985

[Theoretical principles and personal experiences in the use of a sequential stimulation test with arginine, gonadotropin-releasing hormone and thyrotropin-releasing hormone in healthy probands].

The theoretical bases and the performance of a sequential stimulation test (SST) with 0.5 g arginine hydrochloride per kg body weight, 25 micrograms gonadotropin-releasing hormone and 200 micrograms thyreotropin-releasing hormone are described. The result of SST is shown for 17 healthy women with biphasic cyclus. Beside the basal hormone levels B (hypo-, normo- or hyperhormonal) the response A (no, inadaquate or adaquate response) to the stimulation resulting from the peak levels and netto increases is a valuation criteria.

Adolescent↗

PubMed1985

[Effect of ethinyl estradiol sulfonate/norethisterone acetate on gonadotropin secretion in pubertal girls].

In 13 healthy tall girls the influence of the hormonal treatment with depotestrogen ethinylestradiolsulfonate and norethisterone acetate on basal and GnRH stimulated gonadotropin secretion was investigated. The investigation were performed before treatment, during the 8th to 11th cycles of treatment, and in the 4th month after finishing the therapy. LH and FSH serum levels were determined by RIA. Under treatment a suppression both of basal gonadotropin serum levels and of the stimulated LH secretion results. After treatment the mean basal gonadotropin levels are higher than before or during the therapy. The LH release after GnRH stimulation shows the same trend.

Adolescent↗

PubMed1985

[Effect of ethinyl estradiol sulfonate/norethisterone acetate on PRI secretion in pubertal girls].

In 13 healthy tall girls the influence of the hormonal treatment with depotestrogen ethinylestradiolsulfonate and norethisterone acetate was investigated on the basal and TRH stimulated PRL secretion. The investigations were performed before treatment, during the 8th to 11th cycles of treatment, and in the 4th month after finishing the therapy. PRL levels were determined by RIA. Under treatment significant higher basal PRL levels were found than before (p less than 0.05), but never hyperprolactinemic values. The responsibility of the lactotropic cells to TRH stimulation was increased, too.

Adolescent↗

PubMed1985

[Effect of ethinyl estradiol sulfonate/norethisterone acetate on HGH secretion in pubertal girls].

In 13 healthy tall girls the influence of the hormonal treatment with depotestrogen ethinylestradiolsulfonate and norethisterone acetate was investigated on basal and arginine stimulated HGH secretion. The investigations were performed before, during and after finishing the therapy. HGH serum levels were determined by RIA. During the hormonal therapy the mean basal HGH levels were significant higher (p less than 0.05) than before or after therapy. Independent from hormonal therapy there was no stimulation of the HGH secretion by arginine in about 2/3 of the girls.

Adolescent↗

PubMed1985

[Effect of ethinyl estradiol sulfonate/norethisterone acetate on TSH, T4, TBC and the FT4 index in pubertal girls].

In 13 healthy tall girls the influence of the hormonal treatment with depotestrogen ethinylestradiolsulfonate and norethisterone acetate on basal and TRH stimulated TSH secretion, total thyroxine (T4), thyroxine binding capacity (TBC) and free thyroxine index (FT4 index) was investigated. The investigations were performed before treatment, during the 8th to 11th cycles of treatment, and in the 4th month after finishing the therapy. TSH and T4 serum levels were determined by RIA, TBC by radio agent assay, the FT4 index was calculated. The mean basal TSH levels before, during and after therapy showed no significant differences. During hormonal therapy T4, TBC and FT4 were significant higher (p less than 0.05) than before and after therapy.

Adolescent↗

PubMed1985

[Behavior of the hypothalamo-hypophyseal unit to stimulation with arginine, GnRH and TRH in patients with chronic uremia].

In 11 women aged from 20 to 47 years (average age 33,1 years) with chronic uremia, treated by hemodialysis, a sequential stimulation test (SST) with 0.5 g arginine hydrochloride per kg body weight, 25 micrograms GnRH and 200 micrograms TRH was performed to examine the responsibility of the hypothalamo-pituitary unit. For evaluation of basal and stimulated secretion of PRL, LH, FSH, TSH, and HGH the corresponding serum levels were determined by RIA. 10 of the 11 women showed a galactorrhoea. No correlation between levels of PRL and creatinine could be found. Menstrual disorders in women with chronic uremia are discussed in the context of basal LH serum levels nearly always unphysiologically increased. In a few cases disturbances of basal secretion of TSH and HGH, respectively, could be observed.

Adult↗

PubMed1985

[Lactation inhibition with various dosages of lisuride--prolactin secretion and effectiveness].

The influence of lisuride in three several dosages (600, 750, and 900 micrograms) was studied on prolactin secretion and inhibition of lactation in 30 normal postpartum patients. 10 normal nursing postpartum patients served as controls. A rebound effect of prolactin secretion was demonstrable following lisuride medication during 10 days. This effect did not occur after a therapy lasting 15 days. 600 micrograms of lisuride daily showed a good inhibition of lactation and suppression of prolactin secretion. Severe side effects could be only observed during lisuride treatment with a dosage of 900 micrograms.

Adolescent↗