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Biomedical subjects

S Kudo

Publications and source records attributed to S Kudo.

At least 289 records · Page 16Linked to original sources
PubMed1987

Response of blood cells to hemorrahagic shock in the dog.

Response of canine blood cells to hemorrhagic shock was examined using ultrastructural and ultracytochemical techniques. Blood platelets responded to hemorrhagic shock with a decrease of alpha-granules and the simultaneous development of the platelet canalicular system which opened to the extracellular environment, and platelets having no or few granules appeared at the end of the experimental period. Neutrophil leukocytes also responded to shock, with the decrease of both specific and azurophil granules. Eosinophil leukocytes were morphologically unchanged before and after shock but basophils were not found in the present observations. The effects of dexamethasone and phenoxybenzamine on inhibition of blood cell degeneration during hemorrhagic shock were examined. These drugs were considerably effective to inhibit degeneration of blood cells.

Journal Article↗
PubMed1987

Development of cell systems to study viral gene transcription at the initial phase of Epstein-Barr virus infection.

Two infection systems have been introduced in order to study viral gene expression at the initial period of Epstein-Barr virus (EBV) infection which leads to immortalization. The data indicate that major viral gene expression in tonsil lymphocytes at 2 days post-infection (p.i.) with EBV is very similar to that observed in latently infected cells. Both tonsil lymphocyte and BJAB cell (lymphoblastoid cells free of EBV genome) infection with EBV induced similar viral gene transcription. Twelve cDNA clones were prepared from poly(A) RNA of tonsil lymphocytes infected with EBV 2 days p.i. by hybridization with BamHI fragments of EBV DNA. Some cDNAs were derived from primary transcripts of the BamHI-WYHK region, suggestive of splicing of a large transcript. It is possible that a number of cDNA clones may be derived from cellular genes. The derivation of these cDNA clones is being studied.

Bacteriophage lambda↗
PubMed1987

Localization of polysialoglycoprotein as a major glycoprotein component in cortical alveoli of the unfertilized eggs of Salmo gairdneri.

Polysialoglycoprotein (PSGP, 200 kDa), first isolated by S. Inoue and M. Iwasaki in 1978 (Biochem. Biophys. Res. Commun. 83, 1018-1023) from unfertilized eggs of rainbow trout, has been shown to comprise a unique class of glycoproteins associated with the exocytosis of cortical alveoli. In 1986, 200-kDa PSGP was shown to undergo proteolytic depolymerization to 9-kDa PSGP on egg fertilization (activation) and there was an indication that 200-kDa PSGP may possibly be a component of cortical alveoli (J. Biol. Chem. 261, 5256-5261). In this paper we present evidence demonstrating that PSGP is actually a component of cortical alveolus. First, a cortical alveolus-rich fraction (CA fraction) was obtained by low-speed centrifugation of the homogenate of unfertilized eggs of rainbow trout. The 200-kDa PSGP was found to be a major component extractable with buffered saline from the CA fraction by chemical analysis of isolated materials. Treatment of the eggs to induce parthenogenetic activation resulted in all cases in the loss of both cortical alveoli and PSGP in the CA fraction. Second, perivitelline space fluid was isolated from the activated eggs of rainbow trout and analyzed, and 9-kDa PSGP was confirmed to be present as a major proteinaceous component. Third, following incubation of the eggs in water for activation, the time course of the appearance of 9-kDa PSGP and the breakdown of 200-kDa PSGP was observed. The formation of 9-kDa PSGP was detected in the eggs after 1 min of incubation and its level rose rapidly, attaining a maximum at 7 min after incubation. During this period, there was a concomitant fall in the level of 200-kDa PSGP. This formation and rapid increase in 9-kDa PSGP correspond directly to the time course of cortical alveolus exocytosis in activated chum salmon eggs recently studied by scanning electron microscopy.

Animals↗
PubMed1986

Structure of viral DNA in a rat cell line, GY1, transformed by Ad12 HindIII fragment-G.

The cell line GY1, established by transformation of a rat cell line 3Y1 with the Ad12 HindIII fragment-G (leftmost 6.8%, nucleotide 1 to 2322), contains more than 100 viral copies per haploid genome. The viral DNAs in this cell line were cloned into a phage vector, lambda gtWES lambda B, and recloned into pBR322 with their flanking cellular DNAs. Independently isolated 39 clones were analyzed by restriction enzyme cleavage and Southern blot hybridization experiment and divided into 11 classes. Some of classes contained multiple identical clones, at maximum 16 clones. It may be interpreted that amplification of some of the recombined sequences had occurred after the multiple integrations of transfected DNAs within cells. Using five clones from different classes the sequences of recombination sites were determined. Viral DNAs deleted with varying degrees at both ends were flanked by quite different cellular sequences in different clones and no common sequences were revealed around viral-cellular junctions. Tandemly repeated viral DNAs were found in one of the clones to be integrated in a head to tail manner into cellular DNA. The linkage of these two viral DNAs had occurred at the site where parental viral DNAs shared 2 bp. Palindrome structures could be constructed around viral-cellular and viral-viral junction sites and around the regions of parental viral DNAs corresponding to the junction sites in all of the cases investigated.

Adenoviridae↗
PubMed1986

Radiological analysis of hemifacial spasm with special reference to angiographic manifestations.

Fifty-one patients with hemifacial spasm secondary to facial nerve compression by normal appearing, elongated, tortuous and/or dilated arteries, were treated by microsurgical neurovascular decompression. Post-operatively, 91% of 44 of these patients with one to six year postoperative follow-ups were satisfactory. The angiographic findings showed significant increase in the number of common trunk anomalies. Variations of the anterior and posterior inferior cerebellar and vertebral arteries are discussed in relation to nerve compression.

Adult↗
PubMed1986

An experimental evaluation of microcapsules for arterial chemoembolization.

Microcapsules, 106 micron (range, 50-350 micron), of different capsular materials (monoglyceride, monodiglyceride, natural wax, cellulose polymer, or lactic acid polymer) with and without floxuridine (2'-deoxy-5-fluorouridine, FUDR) were intraarterially injected into dog kidneys. The drug-release characteristics of the microcapsules, as determined by analysis of renal and systemic venous blood samples over a 6-hour period, were uniphasic or multiphasic depending on the capsular material. Histologic changes of varying degrees were noted in all kidneys embolized except for those subjected to capsules of the cellulose polymer. The most striking changes were produced by the lactide polymer capsules. The potential applications of microencapsuled chemotherapeutic agents in intraarterial transcatheter treatment of cancer are discussed.

Animals↗
PubMed1986

[Pressure gradient across the mitral valve in mitral stenosis estimated by high pulse repetition frequency Doppler method].

High pulse repetition frequency (HPRF) Doppler and continuous wave (CW) Doppler methods were used to estimate the pressure gradient across the mitral valve. Twenty-two cases of mitral stenosis and five cases of ischemic heart disease were studied. Both the HPRF and CW Doppler studies were conducted during catheterization in all cases. In the Doppler study, pressure gradient was calculated using the simplified Bernoulli's formula. The HPRF device used was a type SSD-730 produced by Aloka Co. It had a reference frequency of 2 MHz. Its minimum pulse repetition frequency was 4.2 KHz; its maximum, 19.2 KHz. Among the 27 cases, the maximum flow velocity measured by the HPRF method at the level of the mitral valve orifice was compared with that by the CW method. As the velocity increased, the discrepancy of measured values between the two methods increased, but it was within 0.1 m/sec. Therefore, there was a good correlation between the HPRF and CW methods (r = 0.98). The pressure gradient between time delay-corrected pulmonary artery wedge pressure and left ventricular pressure was compared with that obtained by the HPRF method. Contrary to our expectations, the correlation coefficient between the two was not so high, and the pressure gradients calculated by the HPRF method tended to be underestimated. For eight patients in whom the left atrial pressure could be recorded, the pressure gradient between the left atrium and left ventricle was compared with that obtained by the HPRF method. There was underestimation, and a good correlation coefficient was obtained. When using pulmonary artery wedge pressure as a substitute for left atrial pressure, one must realize that the time delay varies in every case and that the pressure pulse itself is not the same. When the pressure gradient between the left atrium and left ventricle is used, a good correlation coefficient can be obtained. Therefore, the flow velocity obtained by the HPRF method will reflect the true pressure gradient across the mitral valve. The HPRF method proved to have a potential equal to that of the CW method for estimating mitral valve flow velocity in mitral stenosis, and it may be used as a helpful diagnostic tool.

Adult↗
PubMed1986

[Multidisciplinary treatment of the head and neck cancer--FAR therapy and its modification and application].

Treatment of the head and neck cancer has surprisingly developed in the last decade. The most possible reasons were the development of the more effective anticancer drugs and new combination therapy including immunotherapy. FAR therapy, which was applied to the head and neck cancer in our clinic since 1972, was a combination of vitamin A, 5-FU and radiation. We have already reported the results and the problems of FAR therapy. But, recently we have developed the clinical application of vitamin A to the chemotherapy so as to overcome the resistance of the tumor cells to the anticancer drugs. In this paper, vitamin A was focused as a potentiating agent and the possible material to overcome the resistance to the anticancer drugs.

Adult↗