Search PubMed⌕ Search

Biomedical subjects

S Kristensen

Publications and source records attributed to S Kristensen.

121 records · Page 7Linked to original sources
PubMed1978

A study of skin diseases in dogs and cats. V. The intradermal test in the diagnosis of flea allergy in dogs and cats.

A group of 143 dogs and cats with pruritic skin disease was tested by intradermal injection of a dilute whole cat flea extract. The test results were correlated with the clinical diagnosis (Table I, III), with previous or present findings of fleas on the animals (Table II, III), with the presence or absence of blood eosinophilia (Table IV), and, in animals with clinical flea allergy, with the histological findings in a skin biopsy excised from a clinically affected skin site (Fig. 1). The predictive value of a positive and a negative outcome of the test was assessed graphically by means of calculations described in the Appendix (Figs. 2, 3). It is concluded that the test is sufficiently specific to justify its use in animals where flea allergy is suspected but cannot be proved by routine clinical examination. The histological findings and the nature of the skin reactions suggest that a positive outcome of the test is indicative of an immediate, type I hypersensitivity towards components of the oral secretion of fleas and that this hypersensitivity is mediated by reaginic antibody which to some extent is produced by plasma cells located in skin areas where flea saliva is deposited.

Animals↗
PubMed1977

Adenosine 3',5'-monophosphate in plasma, urine, and native and isoproterenol-stimulated leukocytes of dogs.

Adenosine 3',5'-monophosphate (cAMP) was determined by means of a commercial kit in urine and plasma from 40 hospitalized dogs and in native and isoproternol-stimulated leukocytes from 30 of these animals. Mean urine and plasma concentrations were 6.1 micron and 14 nM, with 95% tolerance limits ("normal values") ranging from 0.0 to 12 micron and 0.21 to 27 nM, respectively. The plasma concentration was approximately half the value previously reported for experimental dogs. The median cAMP content in native leukocytes was 5.9 pmol/10(7) cells. The mean response to isoproterenol was 0.85 pmol/10(7) cells, much less than in human leukocytes. The response was statistically significant (P less than 0.01), but was so small that it is unlikely to be measurably affected in atopic dogs.

Animals↗
PubMed1976

A study of skin diseases in dogs and cats. II. Microflora of the normal skin of dogs and cats.

A study of the microflora of the normal skin was undertaken in 10 dogs and 10 cats. Swabs were taken from the surface of the skin at 7 different sites (Fig. 1). Micrococcus spp., alpha-hemolytic streptococci, and Acinetobacter spp. were found consistently in both species. Staphylococcus aureus was isolated from 9 dogs and 4 cats, and staphylococcus epidermidis from 7 dogs and 5 cats. Beta-hemolytic streptococci, Corynebacterium spp., Bacillus spp., Escherichia coli, Proteus mirabilis, Pseudomonas spp., and Alcaligenes spp. were found sporadically.

Animals↗
PubMedDate not supplied

Mediastinal staging of non-small-cell lung cancer: computed tomography and cervical mediastinoscopy.

The exact indications for computed tomography (CT) of the thorax and mediastinoscopy (MS) in lung cancer still remain incompletely defined. The present study was designed to establish a standard approach to cervical MS for otolaryngologists, who in Denmark are traditionally involved in the staging of non-small-cell lung cancer (NSCLC). Sixty-four potentially operable patients with NSCLC underwent thoracic CT prior to bronchoscopy and cervical MS. Cervical MS alone established the histological diagnosis in 20% of the patients. In diagnosing lymph node metastases in the superior mediastinum, a criterion of 10 mm for abnormal enlargement resulted in an overall sensitivity and specificity of mediastinal CT of 81 and 84%, respectively, and the overall false-negative and false-positive rates appeared to be 10 and 29%, respectively. It could be demonstrated that mediastinal lymph nodes in patients with mediastinal metastases were significantly larger than mediastinal lymph nodes in patients without metastases. No clinicopathological characteristics could be identified to influence the accuracy of CT, except for the finding that the rate of false-negative mediastinal CT was significantly higher in patients with right-sided than in patients with left-sided lesions. It is concluded that because of the relatively low sensitivity and specificity of mediastinal CT, cervical MS remains essential in the evaluation of patients with presumed or verified NSCLC and that cervical MS, in experienced hands, is a safe and accurate procedure. For Danish otolaryngologists, the strategy of routine cervical MS, performed under general anaesthesia in the same stage as bronchoscopy, is advocated as a standard approach to mediastinal assessment for the staging of NSCLC. However, thoraco-abdominal CT is advocated for all patients with NSCLC, in whom operation is contemplated, as a supplementary investigation after other routine diagnostic and staging procedures, including cervical MS, have been carried out.

Adenocarcinoma↗
PubMedDate not supplied

Preeclampsia -- a state of prostaglandin deficiency? Urinary prostaglandin excretion, the renin-aldosterone system, and circulating catecholamines in preeclampsia.

Urinary excretion of prostaglandin E2 (PGE2) and F2 alpha (PGF2 alpha), plasma concentrations of renin, aldosterone, norepinephrine (NE) and epinephrine (E) were determined during pregnancy, 5 days, 3, and 6 months after delivery in preeclampsia, normotensive pregnant, and nonpregnant control subjects. The PGE2 was higher in normotensive pregnant control subjects than in nonpregnant subjects. In preeclampsia, PGE2 was reduced to nonpregnant level. PGF2 alpha was the same in preeclampsia and in normotensive pregnancy, but elevated when compared to the normotensive nonpregnant control group. Plasma concentrations of renin and aldosterone were increased during pregnancy, but considerably less in preeclampsia than during normotensive pregnancy. NE and E were the same as in nonpregnant subjects during both hypertensive and normotensive pregnancy. All parameters were normal 3 months after delivery. There were no correlations between PGE2, PGF2 alpha, plasma concentrations of renin, aldosterone, NE, or E and blood pressure level in third trimester either in preeclampsia or in normotensive pregnancy. PGE2 was positively correlated to plasma concentrations of renin. It is suggested that the lack of renal PGE2 in preeclampsia might be responsible for the decrease in renal blood flow and sodium excretion. It is hypothesized that preeclampsia is a state of prostaglandin deficiency. The changes in the renin-aldosterone system may be secondary to changes in prostaglandin concentration both in preeclampsia and normotensive pregnancy.

Aldosterone↗
PubMedDate not supplied

Photostability of symphatomimetic agents in commonly used infusion media in the absence and presence of bisulfite.

Previous studies have indicated that epinephrine is photodegraded in sulfite-containing solutions by adrenochrome sulfonate. The present study was undertaken to investigate if the same degradation pattern is found for the symphatomimetic compounds isoprenaline, dopamine, and metaraminol. It was found that isoprenaline and dopamine followed the same degradation pattern as epinephrine--they are photodegraded more rapidly in the presence of bisulfite than in its absence, probably by formation of an aminochrome sulfonate. Metraminol was photostable under the given conditions. The photostability of isoprenaline, dopamine, and epinephrine was further investigated in the infusion media sodium chloride (9 mg/ml), glucose (50 mg/ml), Macrodex (dextran 70, 60 mg/ml, sodium chloride 9 mg/ml) and Ringer acetate. The influence of pH, conductivity, viscosity and media concentration were analyzed. In the presence of bisulfite it was found that Ringer acetate had a photodestabilizing effect, while Macrodex had a slight photoprotective effect, on the catecholamines. The pH of the media was shown to be the most important factor, a low pH being protective. Glucose had a destabilizing effect on epinephrine, dopamine, and isoprenaline in the absence of bisulfite.

Dextrans↗
PubMedDate not supplied

A study of skin diseases in dogs and cats. III. III. Microflora of the skin of dogs with chronic eczema.

The microflora of the skin was studied in 10 dogs with chronic eczema without clinical signs of secondary infection (Table I). The skin surface was swabbed at 7 different sites, making a total of 70 swabs, 25 of which were taken from visibly inflamed areas and 45 from apparently unaffected skin (Table II). Staph. aureus, Staph. epidermidis, micrococci, alpha-hemolytic streptococci, and Acinetobacter spp. were found consistently. Ten different Gram-negative bacteria, 3 different Gram-positive bacteria, and 2 yeasts were found to occur sporadically (Table III). Compared to a group of 10 healthy dogs a more prolific growth of aerobic microorganisms, a greater number of sites carrying Staph. aureus, and a higher recovery of Gram-negative transients were found in dogs with eczema (Table IV--VII). Within the group of dogs with eczema the growth of Staph. aureus was significantly heavier from eczematous skin areas than from clinically normal skin (Table VIII). In dogs with non-infective dermatitides the colonization of the skin by potentially pathogenic microorganisms may have to be considered in the clinical handling of these diseases.

Animals↗