Search PubMed⌕ Search

Biomedical subjects

S Kitano

Publications and source records attributed to S Kitano.

At least 379 records · Page 21Linked to original sources
PubMed1985

Functional role of interleukin 1 in periodontal disease: induction of interleukin 1 production by Bacteroides gingivalis lipopolysaccharide in peritoneal macrophages from C3H/HeN and C3H/HeJ mice.

Hot phenol-water-extracted lipopolysaccharide (LPS) from Bacteroides gingivalis 381 was purified by Sephadex G-100 chromatography with Tris buffer supplemented with sodium deoxycholate and EDTA (B-LPS). In the present study, B-LPS was examined for its ability to induce interleukin 1 (IL-1) production, a mitogenic response, and macrophage activation in LPS high-responder C3H/HeN and low-responder C3H/HeJ mice. A significant increase in IL-1 production was observed in C3H/HeN and C3H/HeJ peritoneal macrophages treated with various doses (1.0 to 50 micrograms/ml) of B-LPS. IL-1 production by C3H/HeN macrophages treated with B-LPS (10 micrograms/ml) was about seven times greater than that by C3H/HeJ macrophages. However, the IL-1 production induced by B-LPS (10 micrograms/ml) in C3H/HeN macrophages was four times lower compared with that induced by Escherichia coli O111 B4 LPS. Also, a significant increase in IL-1 production was found in human monocytes stimulated with B-LPS. That B-LPS-induced IL-1 exhibits some molecular weight heterogeneity was indicated from Sephadex G-75 gel filtration profiles. A significant, high mitogenic response by whole spleen cells with 1 X 10(5) to 5 X 10(4) cells of either mouse strain per well treated with B-LPS (10 to 50 micrograms/ml) was observed. However, the response of C3H/HeJ mice was less than that of the C3H/HeN strain. Also, glucose consumption assays indicated that enhanced macrophage activation occurred in C3H/HeN but not in C3H/HeJ mice treated with B-LPS. In light of recent studies showing that IL-1 stimulates bone resorption in a mouse calvaria system and collagenase production in fibroblasts, we suggest that B-LPS-induced IL-1 may play a significant role in the pathogenesis of adult periodontal disease.

Animals↗
PubMed1985

Glial cells in culture of preretinal membrane of proliferative vitreoretinopathy.

Nine preretinal membranes surgically obtained from seven patients with proliferative vitreoretinopathy were cultured in Eagle's minimal essential medium or RPMI 1640 medium, both with addition of 10% fetal bovine serum, in order to assess the proliferative capacity of the cells in the membrane. In addition, glial fibrillary acidic protein (GFAP, protein specific for glial cells) was stained in the cultured cells, by the immunoperoxidase method (peroxidase-antiperoxidase technique). Cellular outgrowth consisted of five types of cells; 1) spindle-shaped cells, 2) flat round or polyhedral cells, 3) semilunar cells, 4) stellate cells and 5) polygonal cells in close contact with each other and extending in monolayer. The spindle-shaped cells, the flat round or polyhedral cells, and the semilunar cells were dominant in all cases, and they showed vigorous proliferation and migration. Many of these cells were GFAP-positive, indicating their glial origin. The stellate cells were GFAP-positive, but they were few in number. The polygonal cells were near the explants and were GFAP-negative; they were thought to be derived from the retinal pigment epithelial cells. It was confirmed that the glial cells are the major cellular component of the proliferative preretinal membrane, and that they show vigorous proliferative capacity. It may be concluded that the glial cells play the major role in the formation of the preretinal membrane in proliferative vitreoretinopathy.

Adolescent↗
PubMed1984

Simultaneous early carcinomas of the ampulla of Vater and the stomach. Report of a case involving chronic inactive hepatitis.

Early carcinoma occurred simultaneously in both the stomach and the ampulla of Vater of a 69-year-old Japanese woman. A tentative diagnosis of early carcinoma of the stomach and acute hepatitis was made and needle biopsy of the liver revealed chronic inactive hepatitis. A carcinoma in the ampulla of Vater was detected by duodenoscopy. Cholangiography did not reveal the biliary tract. Pancreatoduodenectomy was performed and histological study of the resected specimen showed an early carcinoma restricted to the mucosa and an early carcinoma of the ampulla of Vater invading the sphincter of Oddi. There was no apparent metastasis or invasion of surrounding tissues.

Adenocarcinoma↗
PubMed1984

Poorly differentiated adenocarcinoma of the rectum in a nephrotic patient with focal segmental glomerulosclerosis.

We report herein a case of poorly differentiated adenocarcinoma of the rectum occurring in a nephrotic patient with focal segmental glomerulosclerosis. The neoplasm which first appeared to be a submucosal tumor occurred in a 29-year old Japanese man with a nephrotic syndrome for 2 years and 6 months. Autopsy disclosed a large tumor located between the rectum and urinary bladder. Renal specimens showed changes consistent with focal segmental glomerulosclerosis.

Adenocarcinoma↗
PubMed1984

Production of monoclonal antibodies that recognize specific and cross-reactive antigens of Bacteroides gingivalis.

Four monoclonal antibodies directed against Bacteroides gingivalis were established by hybridoma technology. Their reactivity against B. gingivalis, Bacteroides intermedius, and Bacteroides melaninogenicus was detected by the enzyme-linked immunosorbent assay. Three monoclonal antibodies specifically reacted with B. gingivalis. One recognized antigens that were cross-reactive between B. gingivalis and B. intermedius. These monoclonal antibodies provide new tools for antigenic analysis of B. gingivalis.

Antibodies, Bacterial↗
PubMed1984

[Clinical trials of interferon in ophthalmology].

The clinical trials of interferon in ophthalmology have been carried out mainly using a method of topical administration to viral keratoconjunctivitis. Eye infections by herpes simplex virus in humans have been shown to be benefited by interferon. Topical combination therapy of herpetic keratitis by interferon with debridement or other antiviral agents would be more effective. Epidemic keratoconjunctivitis have failed to respond favourably to interferon except some symptoms.

Adolescent↗
PubMed1984

[A case of spontaneous spinal epidural hematoma presenting Brown-Séquard syndrome].

Although there have been a few reports of spontaneous spinal epidural hematoma, it may be difficult to make a correct diagnosis of the lesion because of atypical clinical symptoms. The authors reported a case of spontaneous spinal epidural hematoma presenting Brown-Séquard syndrome, with a review of literature. This 75-year-old male experienced a sudden onset of severe dorsal neck pain during sleep associated with right hemiparesis which deteriorated quickly to hemiplegia, without loss of consciousness, nausea, vomiting and vertigo. The patient has a history of hypertension and has been treated for chronic hepatitis for one year. On admission, neurological examination revealed right hemiplegia with normotensive deep tendon reflexes, and loss of pain and temperature sensations below the level of C5 on his left side. Position sense of fingers and toes was diminished on his right side, and hyperesthesia was recognized at the area of C4 level. Consciousness disturbance, cranial nerve signs, and urinary incontinence were not seen. Right retrograde vertebral angiograms showed no abnormal vascular shadow, even though anterior spinal artery was visible at the level of C8 through C5. Cervical CT scan revealed a left-side dominant extradural high density mass which situated dorsally at around the upper part of C2 body changing it's position to dorsolaterally as it descended to the lower part of C5. On contrast enhancement study, only the margin of the lesion was partially enhanced, which might be the hematoma membrane. On the third day after the onset, laminectomy from C2 to C5 was performed and the epidural hematoma was evacuated.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗