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Biomedical subjects

S Kaul

Publications and source records attributed to S Kaul.

At least 397 records · Page 22Linked to original sources
PubMed1984

Preparation of monoclonal antibodies against DNA-cytosine-5-methyltransferase from human placenta.

Mouse monoclonal antibodies were prepared against DNA-cytosine-5-methyltransferase (EC 2.1.1.37) from human placenta by conventional hybridoma technology. Spleen cells from BALB/c mice immunized with highly purified enzyme were fused to X-63 Ag8.653 mouse myeloma cells. After the hybrid selection in HAT medium individual clones were screened for production of antibodies directed against the enzyme by use of solid-phase ELISA or RIA in which highly purified DNA methyltransferase was either immobilized on microtiter plates or 125I-labeled enzyme was used as a tracer. Positive clones were subcloned, re-screened in the same system and the presence of antibodies directed against DNA methyltransferase was definitively proved in a test system in which the enzyme activity was removed from the solution in immune complexes precipitated by anti mouse immunoglobulin antibodies. From more than 3800 constructed clones 8 were selected which produced antibodies against DNA methyltransferase from human placenta. These antibodies may serve as a useful tool for analysis of DNA methyltransferase structure, intracellular localization and molecular heterogeneity of this enzyme.

Antibodies, Monoclonal↗
PubMed1983

Interventricular septal and free wall dynamics in hypertrophic cardiomyopathy.

Two-dimensional echocardiography was used to analyze interventricular septal and free wall dynamics in eight normal subjects and eight patients with hypertrophic cardiomyopathy. Upper, middle and lower septal and corresponding free wall motion and thickening were analyzed using both fixed and floating reference systems. The lower and midseptal dynamics did not seem to differ significantly between the two groups and the lower septum seemed to move more than the corresponding free wall (probability [p] less than 0.05). The upper septum moved and thickened less than the rest of the septum in both groups (p less than 0.05), but was less dynamic in patients with hypertrophic cardiomyopathy than in normal subjects when the fixed reference system was used (p less than 0.05). It is concluded that the interventricular septum in hypertrophic cardiomyopathy is not akinetic. Previously reported hypokinesia of the septum in hypertrophic cardiomyopathy may be due to sampling of the upper septum by M-mode echocardiography and to the fixed system of reference used by M-mode echocardiography.

Adult↗
PubMed1983

Chronic effects of myocardial infarction on right ventricular function: a noninvasive assessment.

To assess the chronic effects of myocardial infarction on right ventricular function, 48 subjects were studied utilizing radionuclide angiography and two-dimensional echocardiography. Ten were normal subjects (group I), 11 had previous inferior wall myocardial infarction (group II), 10 had previous anteroseptal infarction (group III), 11 had combined anteroseptal and inferior infarction (group IV) and 6 had extensive anterolateral infarction (group V). The mean (+/- standard deviation) left ventricular ejection fraction was 0.66 +/- 0.03 in group I, 0.58 +/- 0.02 in group II, 0.52 +/- 0.02 in group III, 0.33 +/- 0.03 in group IV and 0.33 +/- 0.01 in group V. No systematic correlation between left and right ventricular ejection fraction was observed among the groups. The mean right ventricular ejection fraction was significantly reduced in the presence of inferior myocardial infarction (0.30 +/- 0.03 in group II and 0.29 +/- 0.03 in group IV compared with 0.43 +/- 0.02 in group I [p less than 0.001]). The group II and IV patients also had increased (p less than 0.001) right ventricular end-diastolic area and decreased (p less than 0.001) right ventricular free wall motion by two-dimensional echocardiography. In the presence of anteroseptal infarction (group III), right ventricular free wall motion was increased (p less than 0.05) compared with normal subjects (group I). Thus, the effects of prior myocardial infarction on right ventricular function depend more on the location of infarction than on the extent of left ventricular dysfunction. Inferior infarction was commonly associated with reduced right ventricular ejection fraction and increased right ventricular end-diastolic area. The right ventricular free wall excursion was increased in the presence of anteroseptal infarction, suggested loss of contribution of interventricular septal contraction to right ventricular ejection.

Adult↗
PubMed1983

Atypical echocardiographic and angiographic presentation of a postoperative pseudoaneurysm of the left ventricle after repair of a true aneurysm.

Subsequent to the repair of a true aneurysm from the posteromedial-basal aspect of the left ventricle, a 58 year old man developed a draining wound at the site of the sternotomy. Two-dimensional echocardiography revealed recurrence of the aneurysm at the site of the previous aneurysm repair. This aneurysm had a wide neck and looked similar in appearance to the previous true aneurysm. However, at surgery the patient was found to have a ventricular pseudoaneurysm with a cardiocutaneous fistula.

Cineangiography↗
PubMed1983

The use of human fetal pancreas for transplantation: experimental and clinical results.

Human fetal pancreata (15-22 weeks of gestation) were precultivated in vitro for 7 to 25 days and transplanted subcutaneously to thymusaplastic nude mice and an insulin-dependent diabetic patient. In mice, precultivated xenotransplanted pancreata showed typical transplant growth in the recipients. Histology revealed typical islets formation. Whereas without precultivation human fetal pancreas older than 16 weeks showed no transplant growth, after precultivation human fetal pancreas even up to 22 weeks of gestation developed. Transplantation of a precultivated human fetal pancreas to a diabetic patient was without effect, e.g. the required insulin dose remained unchanged after transplantation and no C-peptide was detectable.

Adult↗
PubMed1981

Studies of the intestinal transfer of coumarin and 7-hydroxycoumarin across guinea pig and rat small intestine.

The mechanism in the intestinal absorption of coumarin and 7-hydroxycoumarin were studied using the in vitro everted and non-everted gut of guinea pigs and rats. The results of the experiments indicated that both coumarin and 7-hydroxycoumarin are absorbed by the passive drug-transfer process. The presence of probenecid/KCN increased the amount of drug transferred across the gut. The mechanism of the latter compounds on the drug-transfer process is still not clear.

Animals↗
PubMed1980

Enzymatic hypermethylation of DNA in mouse-mouse somatic cell hybrids.

Somatic cell hybrids between mouse L fibroblasts (A9 cells) and Ehrlich ascites tumour cells were constructed by use of poly(ethyleneglycol). The hybrids were selected in hypoxanthine/aminopterin/thymidine medium and morphologically different syncaryons were isolated by a micromanipulation. They were simultaneously analysed for their tumourogeneity, a release of certain proteins into the culture medium, the activity of pyrimidine salvage pathways, and the extent of enzymatic DNA methylation. None of these hybrids gave a rise of tumours if transplanted into DBA/2 mice. Two of the hybrids released a protein immunologically cross-reacting with antibody against C-peptide of human proinsulin. Activities of pyrimidine salvage pathways as measured by incorporation of [14C]deoxycytidine into DNA cytosine and thymine, respectively, are different in the analyzed cells. Enzymatic DNA methylation in somatic cell hybrids was significantly elevated as compared to the parental cell lines.

Animals↗
PubMed1978

Ventriculitis, aqueductal stenosis and hydrocephalus in neonatal meningitis: diagnose and treatment.

Five newborns are presented who had meningitis and developed aqueductal stenosis. The diagnosis of obstruction was made by ventriculography during the course of meningitis. A variety of therapeutic measures (use of intraventricular antibiotics, use of reliably diffusible antibiotics, drainage of ventricles) led to varying results. Protocols should be established to assess each modality or combination thereof to help reduce the extremely high morbidity and mortality of this disease.

Anti-Bacterial Agents↗
PubMed1976

Nutritive role of different carbohydrates for the growth and survial of Oryzaephilus mercator Fauvel (Coleoptera).

Recent investigations on the nutritive role of different carbohydrates for the survival and growth of Oryzaephilus mercator show that fructose, glucose, dextrin and glycogen are of high nutritive value, fructose being even better than natural control diet (wheat + 5% yeast). Maltose, soluble starch, mannitol and sorbitol, although support some growth, are not of as good nutritive value as control diet. All pentoses, sorbose, galactose, lactose, raffinose, potato starch, inulin and dulcitol are of no nutritive value.

Animal Nutritional Physiological Phenomena↗