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S Kano

Publications and source records attributed to S Kano.

At least 253 records · Page 14Linked to original sources
PubMed1986

Unusual apparent hyperchloremia induced by long-term abuse of bromide-containing drugs.

Recently, we have observed 8 cases showing unusually high serum chloride (Cl-) levels of 282, 279, 257, 251, 213, 169, 123 and 120 mEq/l respectively, as measured by the ion selective electrical method (ISE). All of these cases showed almost normal Cl- levels by the coulometric method. Five of the patients had been taking Bromide (Br-)-containing drugs for a long period, and were found to have high serum levels of Br- ranging from 0.6 to 11.0 mEq/l (normal level: 0.1 mEq/l greater than). The cause of this abnormal elevation in serum Cl- as measured by ISE was investigated as follows. First, normal human pooled serum was mixed with NaBr solution to give increasing concentrations of Br- ions, which was then analyzed using the ISE method. Results showed that the selective response ratio of Br- against Cl- had values as high as 15.03 with ISE in contrast to values of 1.0 obtained by coulometry. Secondly, 7 healthy volunteers were given 4 commercially obtained Br(-)-containing tablets (Sedes A; Shionogi & Co., Ltd.) every day for 8 days. Their mean serum Cl- values measured by ISE gradually increased from 108 to 112 mEq/l, paralleling increments of mean Br- value from 0.1 to 0.6 mEq/l over 9 days. From these observations, it is concluded that serum chloride values obtained by ISE are easily interfered with by Br-. Paradoxically, however, this fact is often a good indicator of Br- intoxication. The authors therefore recommend clinicians to pay more attention to the Br- intoxication that often occurs from overdosage of Br(-)-containing drugs, some of which are commercially available without prescription.

Adult↗
PubMed1986

Effects of a new dihydropyridine calcium antagonist on vascular smooth muscles, cardiac muscles and [3H]-nitrendipine binding.

The effects of methyl 2,6-dimethyl-4-(2-nitrophenyl)-5-(2-oxo-1,3,2-dioxaphosphorinan-2- yl)-1,4- dihydropyridine 3-carboxylate (DHP-218), a new dihydropyridine calcium antagonist, on vascular smooth muscles, cardiac muscles and [3H]-nitrendipine binding to the cardiac muscle and brain membranes were investigated in vitro. Vascular smooth muscles: Calcium-induced contraction of the rat aorta in high K+ solution was inhibited by DHP-218 with the pA2 value of 9.11. The IC50 value for the inhibitory effects of this compound in high K+-induced and phenylephrine-induced contraction was 6.3 nmol/l and 66 nmol/l, respectively. Vasodilatory effects of this drug on various blood vessels of rabbits contracted by high K+ appeared to a similar extent. The onset of the vasodilatory effect was very slow and the recovery rate of vasodilatory response after washout with the bathing solution containing high K+ or phenylephrine was also very slow. Cardiac muscles: Negative chronotropic and inotropic actions were observed at concentrations more than 30 and 100 nmol/l, respectively. Duration of the plateau phase of normal action potential was shortened and the amplitude and duration of slow action potential were reduced at concentrations more than 1 mumol/l. Very high vasculoselectivity was observed. Displacement of [3H]-nitrendipine binding: The pattern of displacement of [3H]-nitrendipine binding by DHP-218 was very similar to that of other 1,4-dihydropyridines but this compound was about 70 times less potent than nifedipine in [3H]-nitrendipine displacement capacity. These results indicate that DHP-218 has specific vasodilatory action due to calcium antagonism, but association and dissociation rates with tissue and receptors were different from those of nifedipine.

Action Potentials↗
PubMed1985

Regulation of the growth and functions of cloned murine large granular lymphocyte lines by resident macrophages.

Using cloned lines with the morphology of large granular lymphocytes (LGL) from BALB/c mice, we studied the exact requirements for proliferation and their functional characteristics, as well as their regulation. Although these cloned LGL lines were interleukin 2 (IL-2) dependent for growth, experiments using human recombinant IL-2 (rIL-2), known to be active on murine cells, indicated that IL-2 was a necessary but not sufficient factor. Coexistance of normal macrophages in addition to rIL-2 was found to support continuous proliferation of cloned LGL in vitro. This role of macrophages could be replaced by partially purified IL-1 derived from macrophage-conditioned medium. An IL-2 binding assay using 125I-rIL-2 suggested that the role of normal macrophages was to selectively induce and/or maintain high affinity IL-2 receptors (IL-2R) (Kd, 0.2-0.5 nM) without affecting low affinity ones (Kd, 10-30 nM). Functional studies indicated that most of the LGL clones killed various combinations of representative groups of natural killer (NK)-susceptible target cells, including leukemic cells (YAC-1, RL male 1), virus-infected cells (HeLa-measles, HeLa-herpes simplex virus), and normal bone marrow cells (BMC), whereas none of them affected any of NK-resistant target cells, including uninfected HeLa cells. Some of these clones also suppressed in vitro hematopoiesis. Such characteristic cytotoxic spectra, as well as serological phenotypes (Thy-1+, Lyt-1-2-, asialo GM1-positive, T200+, TdT-, Fc receptor-positive) indicated that these LGL clones exactly represent endogenous NK cells, rather than a variety of anomalous killer cells generated in various culture conditions. Although there was significant heterogeneity of cytotoxic spectrum among LGL clones, no clonotypic distribution of specificities was observed. Normal macrophages were found to modulate the functional expression of LGL clones. They augmented the cytotoxic potential of the clones against leukemic and virus-infected targets, but suppressed intrinsic reactivity against normal BMC. Similarly, LGL clones maintained with macrophages showed much less suppressive effect on in vitro hematopoiesis. The present observations on the interaction of cloned LGL and normal macrophages provide a basic explanation for the mechanisms by which the immediate responsiveness to IL-2 of the NK effector system, without exogenous stimulation, and the functional selectivity toward abnormal rather than normal cells, are actively maintained in vivo.

Animals↗
PubMed1985

A murine cell line (2E10.4.13) produces five hemopoietic stimulators, and interleukin-2 and interleukin-3.

An interleukin-2 (IL-2)-independent murine lymphocyte clone (2E10.4.13) with the Thy1+Lyt1+2-T200+ phenotype was separated from the original IL-2-dependent natural killer (NK) cell line (PEC-1). Erythroid burst-promoting activity (BPA), erythropoietin (Ep), granulocyte/macrophage, megakaryocyte and eosinophil colony-stimulating factors (GM-, MK- and Eo-CSF), IL-2 and Interleukin-3 (IL-3) were produced when these cells were stimulated with phorbol myristate acetate (PMA). When the conditioned medium was run through ion-exchange high-performance liquid chromatography, BPA, Ep, GM-CSF, MK-CSF and Eo-CSF were eluted in the same region as IL-3. In contrast, MK-CSF, much of the GM-CSF and half of the Eo-CSF were eluted in a distinct region where no IL-3 was detected. Chemical analyses of the hemopoietic factors derived from a single T inducer clone indicated that all the hemopoietic activities were associated with IL-3 activity. Some CSF activities (GM-, MK- and Eo-CSF) also could be mediated by the distinct molecules from IL-3, evidence that heterogeneous molecules are responsible for CSF activity.

Animals↗
PubMed1985

Survival of patients with tumors of the renal pelvis and ureter: a retrospective study.

A review of 27 patients with renal pelvic tumors and ureteral tumors admitted to the Tsukuba University Hospital was performed retrospectively. Nineteen cases of renal pelvic tumors and eleven cases of ureteral tumors were submitted to the study. These patients were treated mainly by surgery. The rates for 1-year, 3-year, 5-year and 10-year survival were 88.5%, 57.6%, 40.3% and 20.2%, respectively. No obvious relationship between prognosis of renal pelvic tumor and that of ureteral tumors was seen. Pathologically, there was a correlation between the grade and the stage of the tumor. Prognosis was related to the stage of the tumor, but not to the grade of the tumor. Clinically, the patients with positive urinary cytology had a poorer prognosis. It was considered that no relationship existed between the preoperative clinical stage and the postoperative pathological stage. Consequently, the former is not considered a prognostic factor. We have been performing mainly radical nephroureterectomy with regional lymphadenectomy since 1975. The patients who received the operation with regional lymphadenectomy since 1975. The patients who received the operation with regional lymphadenectomy or with adjuvant chemotherapy had a better prognosis. It is concluded that lymphadenectomy is a valuable operation for improving prognosis.

Adult↗
PubMed1984

[Clinical usefulness of serum procollagen-III-peptide in patients with solid tumor].

An attempt has been made to determine the clinical usefulness of serum procollagen-III-peptide (P-III-P) in comparison with serum CEA and AFP in patients with solid tumor. Serum P-III-P levels were measured in 82 cases of carcinoma and 64 cases of benign disease employing an AG Radiochemishes Laboratrium RIAgnost P-III-P kit. Serum P-III-P levels of 148 healthy subjects were 8.5 +/- 2.6 ng/ml (M +/- S.D.), with an upper limit of 13.7(M + 2S.D.). The serum P-III-P level in cases of hepatocellular carcinoma was elevated to 47.5 +/- 97.5 (88.9%). Serum P-III-P levels in cases of pancreatic carcinoma were elevated slightly, whereas those for patients with carcinoma of the stomach, colorectum, esophagus, and breast were low, and their P-III-P positive rates were lower than those of their serum CEA. On the other hand, the serum P-III-P levels in benign diseases involving the liver and biliary tract were higher than those in other benign diseases. Therefore, although serum P-III-P can be a useful marker in hepatocellular carcinoma, it may possibly be difficult to discriminate it from benign diseases involving the liver and biliary tract.

Adult↗