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Biomedical subjects

S K Sharma

Publications and source records attributed to S K Sharma.

At least 541 records · Page 30Linked to original sources
PubMed1984

Affinity chromatography of membrane fragments. Separation of carbonic anhydrase-enriched and -depleted brain membranes.

Chick brain membranes were fractionated by affinity chromatography on AH-Sepharose 6MB linked to p-sulfamylbenzoic acid, an inhibitor of carbonic anhydrase. Two major fractions were obtained. One, designated unbound fraction, representing 42% of the total membrane protein, eluted freely from the affinity adsorbent. A second fraction, designated specifically bound fraction, contained 36% of the total membrane proteins. In addition, 11% of the total membranes bound non-specifically and could be eluted only by the use of shearing forces. Various lines of evidence indicated that the sulfonamide binding site of membrane-bound carbonic anhydrase, in addition to the carbonic anhydrase inhibitor immobilized to AH-Sepharose 6MB, was responsible for the observed specific binding. The specifically bound fraction was highly enriched in carbonic anhydrase while the unbound fraction was completely devoid of this enzyme activity.

Acetylcholinesterase↗
PubMed1984

Protective effect of metoprolol on emetine induced T wave changes in animals.

We studied the protective effect of metoprolol on emetine induced T wave changes in rabbits. None of the rabbits who were beta-blocked before administration of emetine develop the T wave changes observed consistently in the control group. These observations support our clinical impression that emetine-induced T wave changes are produced because of stimulation of peripheral sympathetic nervous system and can be effectively prevented by prior and concomitant beta-blockade.

Animals↗
PubMed1984

Distal ureteral atresia.

Distal ureteral atresia is a rare congenital anomaly of early childhood. Despite the present day diagnostic modalities, the pre-operative diagnosis continues to elude the clinicians. A greatly increased space between the bladder and the rectum in a lateral excretory urogram, in association with renal and bladder alterations, is highly suggestive of distal ureteral atresia. An interesting case in an adult is described: its embryogenesis and the diagnostic problems are discussed.

Adult↗
PubMed1984

Troponin is unlikely to occur in bovine and chick forebrain.

A search for the presence of troponin in brain reveals that troponin is below 0.00037% of total bovine brain soluble protein. Troponin levels were examined using G-actin-linked Sepharose affinity chromatography and 45Ca binding. The chromatographic and 45Ca binding experiments revealed the presence of several actin and calcium-binding proteins, none of which corresponded to any troponin subunit. In addition, troponin was not found in any chick brain subfraction analyzed, and the level of troponin in chick nerve ending cytoplasm enriched for troponin was less than 0.023%. Considering that substantial amounts of myosin and actin occur in brain, these findings indicate that troponin is not likely to be a regulator of putative brain actomyosin interactions. The significance of these results and their relation to proposed models for neurotransmitter release is discussed.

Animals↗
PubMed1984

Haemodynamics, blood gas and metabolic changes after anaesthesia with chloral hydrate and magnesium sulphate in camels (Camelus dromedarius).

The effects of chloral hydrate and magnesium sulphate (1:1, 10 per cent solution) were evaluated in six healthy adult camels. A dose of 1 ml kg-1 was used. These agents produced satisfactory anaesthesia with good muscle relaxation. The changes in the electroencephalogram showed that the anaesthetic effect lasted for 45 to 60 minutes. Tachycardia and hypotension were moderate and within safe limits. Electrocardiogram studies showed ST segment elevation and primary T wave changes. The acid-base status, blood gas tensions, haemoglobin concentration, blood glucose level and the plasma concentration of total protein, sodium, potassium and chloride were not altered.

Anesthesia↗