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Biomedical subjects

S K Sharma

Publications and source records attributed to S K Sharma.

At least 523 records · Page 29Linked to original sources
PubMed1986

Endotoxin detection and elimination in biotechnology.

Endotoxins liberated by gram-negative bacteria are frequent contaminants of aqueous and physiological solutions. Because of their potent biological effects in vivo and in vitro, their detection and removal are essential for the safe parenteral administration of products produced from natural sources, as well as those produced by recombinant DNA technology. Traditional methods of endotoxin detection include the U.S. Pharmacopeia rabbit test and the Limulus amebocyte lysate test. Elimination of endotoxins, however, continues to be a problem. Standard methods of sterilization, such as autoclaving or sterile filtration, have little effect on endotoxin levels. Various techniques for the prevention of endotoxin contamination and endotoxin removal have been discussed. The overall role of endotoxin prevention, detection, and elimination in biotechnology is emphasized.

Endotoxins↗
PubMed1986

Affinity fractionation and characterization of chick brain synaptosomes.

Chick brain synaptosomes were fractionated by affinity chromatography on concanavalin A-Sepharose. Three subfractions were obtained. One, designated UBF, was not bound to the affinity adsorbent and represented 36% of the total synaptosomal protein treated with the beads. A second fraction, designated BF1, adhered to concanavalin A-Sepharose exclusively through its carbohydrate recognition site. The third fraction, called BF2, bound to the beads through hydrophobic interactions and represented about 20% of the total synaptosomal protein. About 20% of the total synaptosomal protein was found to be retarded on three ligand-less gels, with potential for only hydrophobic interactions. This binding can be reversed, however, by ethylene glycol, a result indicating hydrophobic binding sites on the synaptosomes. Enzyme marker studies and electron microscopy showed differences between UBF, BF1, and BF2, mainly with respect to mitochondrial contamination. Binding studies with [3H]-Con A show the absence of Con A-specific carbohydrates from the surface of UBF or BF2. As expected strong and specific binding between [3H]-Con A and [3H] BF1 was observed. These findings are discussed in relation to a model for the interior working of the synaptosomes.

Absorption↗
PubMed1985

Bone metastasis in vulvar carcinoma.

Bone metastasis secondary to vulvar carcinoma, especially that involving distant bone, is an infrequent clinical entity. Three cases are reported in which the patients developed pathologic fractures within 8 months following radical surgery for advanced vulvar carcinoma. The incidence of bone metastasis secondary to vulvar carcinoma is reviewed.

Aged↗
PubMed1985

Solitary kidney with blind-ending ureteral duplication.

The first case of blind-ending ureteral duplication with contralateral renal agenesis is presented. Ureteroureteral reflux due to asynchronous peristaltic activity is presumed to be responsible for functional obstruction and recurrent infection. Surgical intervention offered cure. Relevant literature is reviewed.

Female↗