The effect of vitamin C deficiency on various enzymes of the intestinal epithelium has been studied in guinea pigs. Brush border sucrase and alkaline phosphatase activities were considerably enhanced (p less than 0.001), but leucine aminopeptidase levels were reduced in scorbutic animals compared to the control group. There was essentially no change in the activity of maltase under these conditions. Kinetic studies with sucrase and alkaline phosphatase in control and scorbutic animals revealed that augmentation of the enzyme activities in scurvy is due to enhanced enzyme contents. Lactate dehydrogenase, succinate dehydrogenase, glucose-6-phosphatase and Mg+2 ATPase also exhibited reduced activities in the intestine of vitamin-C-deficient animals. Observed alterations in the activities of intestinal enzymes in scurvy were restored to control levels upon feeding of vitamin C to scorbutic guinea pigs.

Alkaline Phosphatase↗

PubMed1982

Changing morphological spectrum of alpha chain disease--a case report.

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Adult↗

PubMed1981

Effect of vitamin C deficiency in guinea pigs on intestinal functions and chemical composition of brush border membrane.

The effect of vitamin C deficiency on the digestive and absorptive functions of the gut has been investigated in guinea pigs. The absorption of D-glucose was significantly elevated, but that of L-leucine, L-alanine and L-lysine considerably depressed in the intestine of scorbutic guinea pigs compared to controls. The intestinal transport of vitamin B12 was also diminished. Activities of sucrase and alkaline phosphatase on the brush border were enhanced, but that of leucine aminopeptidase markedly reduced in scorbutic animals compared to controls. Maltase activity was unaffected in vitamin C deficient animals. Chemical analysis of the brush borders isolated from scorbutic animals revealed a considerable decrease in membrane protein, total lipids, phospholipids, and free cholesterol contents compared to control animals. In vivo 2-(14)C-acetate incorporation into membrane lipids suggested that the observed decrease in lipid components of the scorbutic membranes is due to reduced synthesis. Administration of ascorbic acid to scorbutic animals ameliorated the intestinal aberrations observed in scurvy.

Animals↗

PubMed1981

Association of Aspergillus fumigatus with sugar-cane bagasse. III. Immunological studies.

In view of the epidemiological significance of Aspergillus fumigatus in allergic bronchopulmonary aspergillosis (ABPA) and its abundance in sugar-cane bagasse the antigenic properties of 11 strains isolated from bagasse have been studied. Three strains isolated from other sources were also included in the study. For comparison sake, A. fumigatus var. ellipticus and A. viridi-mutans were also studied. All the strains of A. fumigatus irrespective of the source of isolation were found to be immunologically potent when tested against sera of a patient of ABPA and hyperimmune sera. Aspergillus viridi-nutans though belonging to A. fumigatus group gave negative results in DID, CIE and skin test. On the other hand, A. fumigatus var. ellipticus showed results comparable to A. fumigatus. The counterimmunoelectrophoresis seems to be more efficacious test than double immunodiffusion for the detection of precipitating antibodies.

Antibodies, Fungal↗

PubMed1980

Effect of a single oral dose of malathion on D-glucose and glycine uptake and on brush border enzymes in rat intestine.

The effect of a single oral dose of malathion (1 g/kg body wt.) on the digestive and absorptive functions of the intestinal epithelium has been investigated in rats. The absorption of glucose and glycine was considerably reduced (35%) inpesticide fed animals compared to controls. The activities of brush border sucrase, lactase, alkaline phosphatase, Mg2+-ATPase and lactate dehydrogenase were also significantly depressed in malathion exposed rats, but there was no change in the leucine aminopeptidase levels under these conditions. Mucosal DNA, RNA and protein contents remained unaltered in pesticide toxicity. These results suggest that malathion toxicity induces functional derangements of the intestine.

Administration, Oral↗

PubMed1980

Efficacy of counterimmunoelectrophoresis in the detection of fungal antibodies in allergic bronchopulmonary mycoses.

The allergic bronchopulmonary mycoses (ABPM) are characterized by dual antibody response which includes immediate Type I and delayed Type III Arthus reactions to fungal antigens. In such cases the demonstration of precipitins is of diagnostic importance. In the present study the efficacy of counterimmunoelectrophoresis (CIE) has been compared with that of conventional agar gel double immunodiffusion technique (DID). Cases of allergic bronchopulmonary aspergillosis (ABPA) and candidiasis (ABPC) were diagnosed in a survey among patients with pulmonary diseases on the basis of positive clinical, radiological, mycological and immunological findings. Precipitating fungal antibodies were detected by CIE and DID techniques in 77 and 59 cases of ABPA and ABPC respectively. The former technique revealed double or multiple bands as against a single band observed in double immunodiffusion in a great majority of these cases. With CIE it was further possible to demonstrate the presence of precipitins in about 65 per cent of suspected cases of ABPM found otherwise negative in DID tests. The CIE has proved to be a more efficacious serodiagnostic procedure in allergic bronchopulmonary mycoses in which antibody levels are known to vary a great deal.

Antibodies, Fungal↗

PubMed1980

Visuo motor coordination time in normal children.

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Child↗

PubMed1979

Kala-azar in north-western India: a study of 24 patients.

Twenty-four sporadic cases of kala-azar diagnosed over an 11-year period in a referral medical centre in north-western India are reported. Most of the patients were residents of non-endemic areas or where endemicity was low. Certain unusual clinical and laboratory features were seen in some of the cases, namely, lymphadenopathy, nasopharyngeal growth, acute and chronic hepatic involvement and portal hypertension. Awareness of the occurrence of the disease and of its protean modes of presentation was found to be an important factor in early diagnosis.

Adolescent↗