The enantiomeric resolution of certain amino acids and some of their dansyl derivatives was achieved on thin silica gel plates impregnated with (1R,3R,5R)- 2-Azabicyclo[3,3,0]octan-3-carboxylic acid, which is an industrial waste material, and a proline analogue non proteinogenic alpha-amino acid. Different combination of 0.5 M NaCl-MeCN were found to be successful in resolving dansyl-DL-amino acids; addition of methanol was required in some cases. Spots were visualised using a fixed wavelength (254 nm) ultraviolet chamber. Resolution of DL-amino acids was successful in different combinations of MeCN-MeOH-H2O and detection was made with ninhydrin (0.2% in acetone).

Amino Acids↗

PubMedDate not supplied

Resolution of enantiomers of amino acids by HPLC.

Application of HPLC as a prime tool in the area of enantiomeric resolution has opened doors of success and varied interest. Use of chiral reagents either indirectly (as derivatization reagent) or directly (added to stationary or mobile phase) has led to achieve resolution of a wide range of compounds. Amino acids, being important molecules with simple structure and easy availability, have been extensively studied. A bibliographic survey on HPLC resolution of amino acids and derivatives along with a brief discussion on general methods of enantiomeric separation has been presented.

Amino Acids↗

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TLC studies on certain aminoglycoside antibiotics.

Aminoglycoside antibiotics were separated by thin layer chromatography. Separation was achieved on untreated silica gel layers using various combinations of solvent systems, dioxane:ammonia, methanol:ammonia and propanol:ammonia. These antibiotics were visualized with the help of iodine vapours.

1-Propanol↗

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Triphenyl tetrazolium chloride (TTC) dye test for quick diagnosis of urinary tract infection.

We evaluate the triphenyl tetrazolium chloride (TTC) dye reduction test for quicker diagnosis of urinary tract infections for its sensitivity and reliability to detect significant bacteriuria. Of the 1400 urine samples tested 780 (55.7%) had significant bacteriuria. TTC dye test was positive in 678 (86.9%) of those with significant bacteriuria thereby showing its usefulness. The test is simple and cheap and can be carried out in field situations.

Bacteriuria↗

PubMedDate not supplied

Detection of tubercule antigen in cerebrospinal fluids by ELISA for diagnosis of tuberculous meningitis.

An Enzyme Linked Immunosorbent Assay (ELISA) was standardised to detect the presence of tubercule antigen in cerebrospinal fluids from patients with meningitis. CSF samples from clinically suspected cases of tuberculous meningitis, culture proven pyogenic meningitis and non-bacterial aseptic central nervous system (CNS) disorders were tested by ELISA to demonstrate its potential utility for routine diagnostic purpose. Tubercule antigen was detected in 73% cases of tuberculous meningitis and was absent in pyogenic and other non-bacterial CNS disorder cases. The test appears to be a promising approach for a definitive diagnosis of tuberculous meningitis.

Antigens, Bacterial↗

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Diagnostic and therapeutic evaluation of bronchoscopy.

The diagnostic and therapeutic efficacy of bronchoscopy was determined in 85 children. The major indications were foreign body removal in younger patients and evaluation of tracheobronchial pathology in older children. Foreign body was commonly isolated in the toddlers and even in the elder age group (19%) as well as in those with suspected ingestion (44%). Groundnut was the predominant foreign body and right bronchus was the most frequent site. Tracheobronchitis (27%), bronchiectasis (13%) endobronchial tuberculosis (9.4%) and mucus plug (3%) were the other frequent findings. Bronchography was performed in 16 patients and it confirmed the diagnosis in 75% of the cases. Minor complications were encountered in 8% of patients. In the present study bronchoscopy yielded definite results in 83% and in many, including those with normal findings it guided further management.

Adolescent↗

PubMedDate not supplied

Nutritional intervention among rural pre-school children--an evaluatory study.

Explore the source record for details and available documents.

Body Weight↗

PubMedDate not supplied

Indian experience of home based mothers card: ICMR task force study.

To improve the quality of MCH services, a Home Based Mothers Card (HBMC) prepared and recommended by World Health Organization was adapted to Indian situation, and introduced in 1.5 lakh population of rural area covered by 6 participating centres under the aegis of Indian Council of Medical Research. Two thousand four hundred and forty six mothers were given this card and were followed up for a period of 2 years. Only 89.2 percent retrieval of the cards was possible after a period of 18 months. Screening of the population for "at risk" women monitoring and referral could be undertaken with the help of this card. Improved antenatal, and referral services were observed during the study period. The card (HBMC) was acceptable to the mothers as well as to the health workers, as a tool for improving the quality and coverage of MCH services being rendered at the Primary Health Centre.

Feasibility Studies↗

PubMedDate not supplied

Schneiderian symptoms and childhood trauma in the general population.

The Dissociative Disorders Interview Schedule (DDIS) was administered to a sample of 502 adults in the city of Winnipeg, Manitoba, Canada. Findings indicate that Schneiderian symptoms are highly related to childhood trauma and other dissociative symptoms clusters in the general population, as they are in clinical populations. Implications of the findings are discussed.

Adult↗

PubMedDate not supplied

A gene affecting the specificity of the chemosensory neurons of Drosophila.

The sensilla on the proboscis and tarsi of Drosophila contain five neurons, four chemosensory and one mechanosensory. The sugar-sensitive neuron, designated S, carries independent acceptor sites for pyranose, furanose and trehalose. Two others, L1 and L2, respond to salts. The fourth neuron, W, is inhibited by salts and sugars, and is believed to mediate detection of water. We describe here a gene in which mutations alter the neurons in such a way that the S cell is excited by salts. As a result, the mutant flies are strongly attracted by NaCl at concentrations which are repellent to the wild type. To our knowledge, this is the first instance of a mutation which changes the specificity of the chemosensory neurons.

Alleles↗