Search PubMed⌕ Search

Biomedical subjects

S Ivanov

Publications and source records attributed to S Ivanov.

At least 199 records · Page 11Linked to original sources
PubMed1984

Multiple forms of chicken liver and hepatoma Mc-29 microsomal and plasma-membrane sialyl and fucosyltransferases.

Multiple forms of microsomal and plasma membrane sialyl and fucosyltransferases from chicken liver and transplantable hepatoma Mc-29 have been separated by means of isoelectric focusing. A net different pattern was distinguished between liver and hepatoma microsomal and plasma-membrane associated transferases. Microsomal sialyltransferase from hepatoma Mc-29 has typical forms with pI = 5.69, 7.43, 8.05 and 8.56, while in plasma membrane, enzymes with pI = 5.00 and 8.70 occur. The presence of 9 forms of fucosyltransferase within the pH range 3.46-9.57 for hepatoma microsomes and within pH 4.52-9.60 for plasma membranes was detected. Forms with pI 5.10, 5.75 and 7.87 could be considered specific for the hepatoma microsomal enzyme, and forms with pI 4.52, 4.85 and 5.20 for the plasma-membrane associated enzyme.

Animals↗
PubMed1984

Comparative study on fucosylation of chicken liver and virus-induced hepatoma Mc-29.

Comparative studies on fucoprotein metabolism of chicken liver and hepatoma Mc-29 have been carried out and the following parameters were determined: the incorporation rate of [14C]fucose into hepatoma and liver total tissue homogenate, acid-soluble and acid-insoluble fractions, acid-soluble nucleotide fraction and into plasma-membrane acid-precipitable fraction; the activity of microsomal and plasma-membrane fucosyltransferase; the electrophoretic pattern of hepatoma and liver plasma-membrane proteins and the incorporation of [14C]fucose into the glycoprotein fractions in both plasma-membrane preparations. It was found that the labelling of hepatoma tissue homogenate and plasma membranes was higher than that of the same liver preparations 3 hr after the [14C]fucose injection. This finding was supported by a considerably elevated hepatoma fucosyltransferase activity. The labelling rate of numerous fucoproteins from hepatoma plasma membranes was greatly increased and some of the individual glycoprotein bands were labelled to a higher extent compared with liver. The data presented show specific alterations of fucose and fucoprotein metabolism which could be considered as a characteristic feature of chicken viral-induced hepatoma Mc-29.

Animals↗
PubMed1984

[Case of a biliodigestive fistula--a variant of Bouveret's syndrome].

The female patient N. I. G., aged 65, case report No 21336/416 was admitted to the Clinic of Gastroeneterology on October 12, 1982, with the following diagnosis: Cholelithiasis, Cholecystitis chronica calculosa, Fistula biliodigestiva. The anamnestic data, the objective examination as the data from operation protocol revealed that the case reported was a peculiar, very rare, favourable evolution of Bouveret syndrome. The biliary concrements (the biggest 10 X 15 mm), that had passed through the fistula opening into the permanently deformed and constricted duodenal bulb have twice caused short-term disturbed intestinal passage. The patient, with the vomited juices, had thrown out the concrements that caused the obturation, resulting into restoration of the intestinal passage and clinical improvement. The surgical operation was performed 57 days after the acute incident for removing of gallbladder and elimination of the fistula passage.

Aged↗
PubMed1983

Effect of D-glucosamine on the content and synthesis of UDP-sugars and plasma membrane associated carbohydrates in chicken liver and hepatoma Mc-29.

1. Comparative studies on the effect of D-glucosamine on the synthesis of UDP-sugars and on the incorporation rate of [14C]glucose into neutral sugars, sialic acid and glucosamine isolated from chicken liver and hepatoma Mc-29 plasma membranes have been carried out. 2. The influence of D-glucosamine on the activity of microsomal UDP-N-acetylglucosamine: glycoprotein N-acetylglucosaminyltransferase to lipid (dolichol monophosphate) and to protein acceptor was studied too. 3. It has been shown that D-glucosamine provokes alterations in the content and synthesis of UDP-sugars and it is an inhibitor of the glycosylation processes. An inhibitory effect of this sugar analog on the N-acetylglycosaminyltransferase has been established.

Animals↗