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Biomedical subjects

S Imre

Publications and source records attributed to S Imre.

At least 19 recordsLinked to original sources
PubMed2001

Characterization and lime treatment of olive mill wastewater.

Seventeen olive mill wastewater (OMW) samples were characterized. For characterization of the samples, amount of total, fixed, volatile, suspended, volatile-suspended solids, COD, oil-grease, polyphenol, volatile phenol, nitrogen and reducing sugar were determined. Effects of lime treatment on the waste samples were investigated. Reduction of contents of the samples treated with the lime was described. The effect of the addition of lime on an artificial phenolic mixture was also examined.

Calcium Compounds↗
PubMed2001

High sensitivity to autoxidation in neonatal calf erythrocytes: possible mechanism of accelerated cell aging.

The suspension viscosity, formation of methaemoglobin and production of malondialdehyde (MDA) associated with the non-enzymatic oxidation of polyunsaturated fatty acids during auto-oxidation conditions in vitro have been compared in erythrocytes from young calves (2, 4 and 6 weeks of age) and mature cattle. The autoxidation conditions were designed to simulate the oxidative stress to which neonatal erythrocytes are exposed in vivo. Characterisation of lipid peroxidation was also undertaken by a combination of lipid fluorescent measurements and quantification of the superoxide dismutase (SOD) activities of the erythrocytes. The results demonstrated that high SOD activities in the erythrocytes of the neonatal calf was insufficient to afford protection against the increased autoxidation of haemoglobin and subsequent accumulation of lipid peroxidation products. High levels of methaemoglobin formation and lipid peroxidation were able to provide an explanation for an observed reduction in rheological adaptability (increased suspension viscosity) and an accelerated aging of the neonatal cells under in vivo conditions.

Animals↗
PubMed2000

Reduced lipid peroxidation capacity and desaturation as biochemical markers of aging.

The lipid peroxidation capacity, the lipid composition and the fatty acid composition of the major lipid fractions have been compared in the liver tissue of mice at different ages. Compared with mice at 2 months of age, there was a notable reduction in peroxidation capacity in the livers of mice at 12 and 26 months of age. These observations were associated with changes in the proportions of the major C18, C20 and C22 polyunsaturated fatty acids expressed in all the major lipid fractions and were accompanied by alterations in lipid composition. From the relationship between these features it is suggested that, under in vivo conditions, regulation of the aging processes may be intimately related to the metabolism of the polyunsaturated fatty acids and associated aspects of lipid metabolism.

Journal Article↗
PubMed1993

[Effect of paracetamol on the acid hemolysis of erythrocytes, with special reference to the intracellular glutathione level].

The authors give account of their study concerning the effect of paracetamol on the acid haemolysis, and glutathione content of human erythrocytes. In vitro, paracetamol decreased intracellular glutathione content in a dose dependent manner. This may lead to an increased sensitivity of erythrocytes to the haemolytic effect of hydrochloric acid, what may explain the dose dependent decrease of time of haemolysis, presented in form of dynamic haemolysis curve. In vivo four hours following the oral intake of 500 mg paracetamol, although the intracellular glutathione content did not decrease significantly, shortening of the time of acid haemolysis could be demonstrated in more than half of the persons studied. No haemolysis was caused by the given dose of the drug.

Acetaminophen↗
PubMed1992

The effect of silibinin (Legalon) on the the free radical scavenger mechanisms of human erythrocytes in vitro.

The effect of Legalon was investigated parallel with that of Adriblastina (doxorubicin) and paracetamol on some parameters characterizing the free radical scavenger mechanisms of human erythrocytes in vitro and on the time of acid hemolysis performed in aggregometer. Observations suggest that Adriblastina enhances the lipid peroxidation of the membrane of red blood cells, while paracetamol causes significant depletion of intracellular glutathione level, thus decreasing the free radical eliminating capacity of the glutathione peroxidase system. Legalon on the other hand, is able to increase the activity of both superoxide dismutase and glutathione peroxidase, which may explain the protective effect of the drug against free radicals and also the stabilizing effect on the red blood cell membrane, shown by the increase of the time of full haemolysis.

Acetaminophen↗
PubMed1989

Lipid peroxidation and superoxide dismutase activity in muscle and erythrocytes in adult muscular dystrophies and neurogenic atrophies.

Lipid peroxidation (LP) and superoxide dismutase (SOD) activity were determined in erythrocytes and skeletal muscle obtained from patients with limb-girdle and facioscapulohumeral muscular dystrophies, neurogenic atrophies and from age-matched control subjects. Neither lipid peroxidation nor SOD activity in erythrocytes of patients differed from control values. SOD activity and LP in muscle specimens were also normal in types of neurogenic atrophy. Lipid peroxidation in the muscle from patients with adult types of muscular dystrophy had a tendency to be increased. The values were widely scattered, the highest being obtained in the older patients with long duration of disease.

Adult↗
PubMed1988

Effect of 2-mercaptoethanol on some metabolic indices of ageing of CBA/Ca inbred mice.

It has been shown in several studies that 2-mercaptoethanol (2-ME) improves the life span and aspects of life performance of laboratory animals. To obtain further details on the beneficial effects of 2-ME, a long-term study has been performed on male CBA/Ca inbred mice treated with this antioxidant. Four month-old mice were each given 4 micrograms of 2-ME in physiological saline via intraperitoneal (i.p.) injection 3 x per week. Measurements were made of the following: cold tolerance (heat performance), apparent total body protein turnover (T1/2), changes in the major lipid and fatty acid compositions of the liver, superoxide dismutase activity and formation of malondialdehyde and observations on a range of pathological changes. It was found that the basal rectal temperatures of the treated mice were higher and in the oldest group, heat performance capacity was better than those of the controls. After about 1 year of age the apparent biological half-life time of total body protein (T1/2) was observed to be shorter in the treated mice. Significant increases were observed to occur in the proportions of the polyunsaturated fatty acids in the lipids of the liver in the mice injected with 2-ME. Although no differences were observed in the superoxide dismutase activities, malondialdehyde concentrations in the livers of the experimental mice were significantly increased. Autopsy data showed that Dunn-sarcomas associated with amyloidoses occurred more frequently in the untreated mice.

Aging↗
PubMed1987

The effect of oxidative stress on inbred mice of different ages.

The effect of oxidative stress applied for 2 months was compared in adult (14-month-old) and aged (27-month-old) female BALB/c mice. The stress was administered in two ways. One group of animals received a high dose of H2O2 (0.5% H2O2 in drinking water) the other one was given a low dose of H2O2 (0.4 ml of 0.5% H2O2 by esophageal cannula every alternate day). After a 2-month treatment the activity of superoxide dismutase (SOD), catalase and lipid peroxidation capacity were measured in liver homogenates of mice. The activity of catalase and capacity of lipid peroxidation gradually decreased as a function of age while the SOD activity did not reduce even in older mice. The aged-mice could tolerate a high dose of H2O2 mainly by increasing SOD activity. As a result of intake of low dose of H2O2 in old animals SOD activity was decreased and lipid peroxidation capacity was getting higher suggesting that in this life period the liver tissue is very sensitive to chronic, oxidative stress effects of low dose.

Administration, Oral↗
PubMed1984

Lipid peroxidation and superoxide dismutase activity in muscle and erythrocytes in Duchenne muscular dystrophy.

Lipid peroxidation and superoxide dismutase activity in muscle and erythrocytes obtained from patients with Duchenne muscular dystrophy (DMD) and controls matched for age and sex were determined. The lipid peroxidation was found to be significantly higher in muscle of the DMD patients but not in erythrocytes. The possible significance of the findings have been considered and discussed.

Child↗
PubMed1984

Superoxide dismutase, catalase and lipid peroxidation in liver of young mice of different ages.

The lipid peroxidation and activities of main enzymes involved in the peroxide metabolism were measured at resting conditions and after completion of a 2-month treatment with H2O2. At the start of the experiments 1- and 3-month-old female BALB/c mice received either a high dose of H2O2 (0.5% H2O2 in drinking water; intake 12.5 x 10(-3) g/day/mouse) or a low dose of H2O2 (0.5 ml of 0.5% H2O2 by esophageal canula on every alternate day; intake 1.0 x 10(-3) g/day/mouse). The following conclusions can be drawn: a relative higher increase in activity of catalase could be induced at the age of 3 months than at the age of 5 months by a high dose of H2O2. The activity of superoxide dismutase (SOD) was not changed by a high dose of H2O2. A moderate increase in the activity of catalase and a remarkable decrease in SOD-activity resulted from a low-dose H2O2 treatment at the age of 5 months. The level of lipid peroxidation was increased by the low-dose and not influenced by the high-dose H2O2 treatment in 5-month-old-mice.

Aging↗
PubMed1984

Quantitative modification of immunohaemolytic sensitivity of bovine erythrocytes.

Experimental work was carried out to study the effect of periodate and neuraminidase on the J-specific and non-J-specific immunohaemolysis of bovine erythrocytes. The method of quantitative immunohaemolysis was applied for the determination of immunohaemolytic sensitivity. Both the periodate and neuraminidase treatment, while increasing J-specific immunohaemolysis, resulted in non-J-specific haemolysis. Periodate treatment that followed a neuraminidase one, however, decreased the enhancement of J-specific immunohaemolysis and resulted in a significant increase of non-J-specific haemolysis. The maximum increase of non-J-specific haemolysis was found in erythrocytes highly sensitive to anti-J-serum.

Animals↗
PubMed1983

Comparative study of the transition temperature of calf and adult cattle erythrocytes.

In our study we have compared the temperature dependence of osmotic fragility in calf and adult cattle erythrocytes. The temperature dependence of osmotic fragility has revealed inflections in calf red cells generally at 20 degrees C (293 K), in adult cattle red cells at about 30 degrees C (303 K). One possible explanation for the lower transition temperature of calf erythrocytes is that the microviscosity of the protein-lipid boundary might be considerably less in the membrane of the calf cell than in the adult cell.

Animals↗
PubMed1982

Comparative study of transition temperature in adult and newborn human red blood cells.

Hypotonic haemolysis of newborn and adult human red blood cells (RBC) is more intensive at 4 than at 37 degrees C. Newborn RBC are more resistant to the decrease of temperature than adult RBC. Transition temperature of the RBC membrane can well be determined by illustrating osmotic haemolysis as a function of temperature. This parameter characterizes well the microviscosity of the membrane. The inclination point of Arrhenius curves representing transition temperature occurred always at 25 degrees C both in the case of adult and newborn RBC. The similarity of the transition temperature indicates the similarity of membrane microviscosity of newborn and adult human RBC.

Adult↗
PubMed1980

Membrane stabilizing effect of lysolecithin in calf red blood cells.

Lysolecithin sensitivity of red blood cells of 4--6 weeks old calves and 5--10 years old cattles was compared in the presence of 1 and 10 microM lysolecithin. When the incubation temperature of red blood cell suspension was increased from 0 or 22 degrees C to 37 degrees C, the addition of 1 microM lysolecithin resulted in reduction of haemolysis and relative stabilization of the membrane as compared to that observed in lysolecithin-free medium. This stabilizing effect was found first of all in calf red blood cells. This effect is hardly detectable in adult cattle red blood cells. ATP depletion resulted in reduction of the stabilizing effect of lysolecithin in calf red blood cells as well. In the presence of 10 microM lysolecithin elevation of the incubation temperature from 22 degrees C to 37 degrees C enhanced the haemolysis of calf red blood cells, while the haemolysis of adult cattle red blood cells was practically not affected. The present results indicate that lysolecithin sensitivity of red blood cells is remarkably different in new-born and adult animals.

Adenosine Triphosphate↗
PubMed1979

Comparative study on the metabolism and filtrability of red blood cells of the calf and adult cattle.

Filtrability of calf and adult cattle red blood cells was studied to characterize their durability; to compare their metabolism, lactate dehydrogenase, glucose-6-phosphate dehydrogenase and glutamate-oxalacetate transaminase activity and ATP-content were determined. The activity of the enzymes declined gradually with age. The most pronounced decline of activity, particularly in the case of lactate dehydrogenase, was observed 6--8 weeks after birth. ATP content of calf red blood cells was ten times higher than that of the adult cattle, however, it proved to be less stable. Filtrability of fresh calf and adult cattle red blood cells was identical, while following incubation for 15--24 hours the decrease of filtrability was more pronounced in calf red blood cells indicating a lower filtrability and, consequently a shorter expected lifetime.

Adenosine↗
PubMed1978

The effect of dbcAMP on the lysolecithin induced hemolysis of calf and adult cattle erythrocytes.

The calf erythrocytes have an increased sensitivity against lysolecithin as compared to their adult counterparts. 10(-3)M dbcAMP increases the hemolysis induced by 5 microgram of lysolecithin in 0.15 M NaCl containing 10 mM phosphate buffer (pH 7.4). By increasing the level of phosphate buffer (75 mM) in the incubation mixture, 10(-3)M dbcAMP decreases the hemolysis induced by 5 microgram of lysolecithin. These data suggest a dual effect exerted by dcbAMP: the relatively labilizing or stabilizing effect prevails as a function of exogenous inorganic phosphate level. 10(-6)M dcbAMP also has a relative protective effect against lysolecithin. The combined addition of cAMP (10(-3)) and theophyllin (10(-4)M) does not stabilize the membrane. By increasing the level of lysolecithin to 20 microgram/ml the stabilizing effect of dcbAMP disappears. DbcAMP (10(-3)) as well as cAMP (10(-3)M) and theophyllin (10(-4)M) have a minimum increasing effect on hemolysis in the absence of lysolecithin, too.

Age Factors↗