Search PubMed⌕ Search

Biomedical subjects

S Imamura

Publications and source records attributed to S Imamura.

At least 487 records · Page 27Linked to original sources
PubMed1981

[A study on luteal function during consecutive cycles (author's transl)].

UNLABELLED: It is conceivable that luteal function is varied with different menstrual cycles. A study was therefore undertaken to investigate for changes in luteal function during consecutive menstrual cycles. METHODS: From those women receiving ambulatory treatment at the hospital 30 displaying a biphasic basal body temperature (BBT) curve were chosen for the study. BBT records were obtained from each of these women for at least 3 consecutive cycles and a total of 194 cycles thus rendered available were evaluated for the pattern of BBT, the duration of high phase (DHP), high phase score (HPS) and planimetric luteal index (PLI). Twenty of these 30 women were subjected further to endometrial dating at the 7th-8th day of high phase with simultaneous determinations of serum estradiol (E2), progesterone (P) and prolactin (PRL) during 2 consecutive cycles. RESULTS: The incidences of abnormal values of the parameters for at least one of the consecutive cycles studied were: DHP; 13.3% pattern; 50-100%, HPS; 40.0%, PLI; 13.3%, E2; 25%, P; 25%, PRL; 30%, endometrial dating; 65%. These results indicate a considerable variability of luteal function.

Body Temperature↗
PubMed1981

Potassium iodide in the treatment of erythema nodosum and nodular vasculitis.

Twenty-nine patients with erythema nodosum, nodular vasculitis, or erythema nodosum-like lesions associated with Behçet's syndrome were treated with potassium iodide. Administration of the drug for systemic effect showed a substantial effect in 11 of 15 patients with erythema nodosum, seven of ten with nodular vasculitis, and one of four with leg lesions of Behçet's syndrome. Relief of subjective symptoms, including tenderness, joint pain, and fever, occurred within 24 hours. Substantial improvement in the eruption occurred within a few days, and the lesions disappeared completely ten to 14 days after therapy was initiated. The patients to whom the medication was administered shortly after the initial onset of erythema nodosum seemed to respond most satisfactorily. The effect of the drug was marked in the patients with positive C-reactive protein reactions, joint pains, and/or fever. Possible mechanisms by which potassium iodide exerts its effect are discussed.

Adult↗
PubMed1980

Immunohistochemical localization of cyclic nucleotides in guinea pig lip.

The cellular localization of cAMP and cGMP in the guinea pig lip was investigated with an indirect immunofluorescence technique. cAMP was distributed both in cell membranes and cytoplasmic elements with coarse and granular intranuclear fluorescence, while cGMP was localized in nuclear sites including nuclear membranes as well as cell membranes and cytoplasm. In the basal portion, both cAMP and cGMP fluorescence was weaker in cytoplasm and cell membranes than seen in the upper portion.

Animals↗
PubMed1980

Benign and neoplastic eosinophilic staining cells: an immunofluorescence study.

Deposition of immunoglobulins, complement and fibrinogen on eosinophilic staining cells was investigated using direct immunofluorescence techniques. Serum factor deposition was detected on benign epidermal eosinophilic cells seen in pityriasis lichenoides et varioliformis acuta, sunburn erythema and, in addition, on subepidermal hyaline bodies in lichen planus; no such deposition occurred on neoplastic eosinophilic cells in Bowen's disease and squamous cell carcinoma. The qualitative findings of immunofluorescence microscopy seem to be different in inflammatory and malignant dermatoses.

Complement System Proteins↗
PubMed1980

Erythema multiforme: demonstration of immune complexes in the sera and skin lesions.

In twenty patients with erythema multiforme we investigated circulating immune complexes and their deposition in the skin lesions. CIq-binding activity was elevated in ten of twenty patients, and the platelet aggregation titre was high in three of twelve tested sera. Decreased levels of C3 were seen in two and of C4 in one out of eighteen patients. Direct immunofluorescence showed a deposition of C3, IgM or IgG in the blood vessel walls of the upper dermis in four of twelve patients. These findings may suggest that transient production of circulating immune complexes and their deposition play an important role in the pathogenesis of this disease.

Adolescent↗
PubMed1980

Treatment of acute febrile neutrophilic dermatosis (Sweet's Syndrome) with potassium iodide.

6 cases with acute febrile neutrophilic dermatosis (Sweet's syndrome) responded rapidly and dramatically to treatment with potassium iodide. All patients became afebrile and symptom-free within 24--48 h after therapy. The cutaneous eruptions subsided completely in 3--5 days. 5 of the patients received the drug only for 2 weeks, but they have had no recurrences. Although the remaining 1 patient had shown minor recurrences after cessation of the medication, he was free of all symptoms during the therapy. The mode of the action of potassium iodide is also discussed.

Adult↗
PubMed1980

Hallermann-Streiff syndrome.

Only 2 cases of Hallermann-Streiff syndrome were reported in the past in the dermatological literature. We describe a 20-year-old female patient whose clinical findings mostly fall into this syndrome, except for the presence of slightly low-set ears. The mother of this patient suffered from rubella and took drugs in her 3rd month of pregnancy. This might have been related to the development of the abnormalities of this patient. Chromosomal analysis was normal.

Adult↗
PubMed1980

Acute generalized pustular bacterid and immune complexes.

The case is described of a 39-year-old man with acute generalized pustular bacterid and high Clq-binding activity in the sera during the active stage. Histamine-induced vascular changes studied by immunofluorescence microscopy revealed a perivascular deposition of IgM and C3. These findings support the view that leukocytoclastic vasculitis underlying the subcorneal pustules is mediated by immune complex deposition.

Adult↗
PubMed1980

Demonstration of circulating and tissue-fixed immune complexes in cutaneous necrotizing vasculitis.

Simultaneous demonstration of circulating and tissue-fixed immune complexes was attempted in 22 patients with cutaneous necrotizing vasculitis (7 anaphylactoid purpura, 9 cutaneous allergic vasculitis, 2 livedo reticularis, 1 thrombophlebitis, 2 erythema elevatum diutinum and 1 acute generalized pustular bacterid). In 16 out of the 22 patients, particularly patients with anaphylactoid purpura and cutaneous necrotizing vasculitis, there was a high Clq-binding activity. Decreased levels of C3 and C4 were seen in 2 and 3 patients, respectively. In 11 out of 16 skin lesions, the granular deposits of immunoglobulins and/or complement were demonstrated in the blood vessel walls of the dermis. IgA deposit was seen in anaphylactoid purpura, and IgM deposit in other types of vasculitis. C3 deposit was the most frequently noted. There was no definite correlation between Clq-binding activity and tissue deposits.

Antigen-Antibody Complex↗
PubMed1980

Immune complexes in patients with drug eruptions: the relationship between skin lesions and circulating immune complexes.

The relationship between skin lesions and immune complexes was studied in sixty-two patients with exanthematic drug eruptions. By means of C1q-binding, conglutinin-binding and platelet aggregation tests, immune complexes were detected in a considerable number of sera from these patients. Patients with widespread maculopapular drug eruptions were found to show a relatively high serum level of immune complexes. There was a close association between the disease activity and the amount of the circulating immune complexes. By immunofluorescence, six of seventeen patients with drug eruptions were shown to have deposits of IgG, IgM, IgA or C3 in the skin lesions. These results suggest that certain immune complexes may play a role in some types of drug eruption as a pathogenetic factor.

Antigen-Antibody Complex↗
PubMed1980

[A study on the significance of bromocriptine (CB-154) administration in normoprolactinemic anovulatory women (author's transl)].

UNLABELLED: The ovulation-inducing effect of bromocriptine (CB-154) and the underlying mechanism in the management of anovulatory infertility with normoprolactinemia were investigated. Thirty-seven normoprolactinemic women (anovulatory cycle, 11 cases; 1st grade amenorrhea, 18; and 2nd grade amenorrhea 8) with anovulia showing plasma prolactin levels of less than 25 ng/ml were give CB-154 daily in doses of 2.5 mh from the second day of menstruation or withdrawal bleeding (1) to assess the ovulatory induction rate, (2) to determine the serum PRL, LH, FSH and E2 levels before and during the drug administration, (3) to measure the serum PRL concentration by TRH test prior to CB-154 medication and (4) to assess size heterogeneity of serum PRL with the use of lyophilization. RESULTS: (1) Ovulation occurred in 21 of the 37 patients, of a total of 42 menstrual cycles studied, 33 were ovulatory, indicating a high rate of repeated success. (2) There was a significant decrease of serum PRL following administration of CB-154, and the finding was the same in the group of women with successful ovulatory induction. Serum LH, FSH and E2 levels did not show any significant change following CB-154 therapy. (3) Prl concentrations were suppressed more in cases of unsuccessful induction than those in successful induction. (4) PRL responses to TRH tended to be lower in cases of successful induction than those in unsuccessful cases. (5) The Little PRL content ratio decreased after administration of CB-154, and this data have suggested that not only the problem of immunologically measured serum PRL levels but the qualitative problems also bear relations to the effect of this hormone on the endocrinological environment.

Amenorrhea↗
PubMed1980

Isolation and physicochemical properties of human chorionic gonadotropin of hydatidiform mole.

HCG of chorionic tissues of hydatidiform mole was purified using ammonium sulfate fractionation, ion exchange chromatography and gel filtration. The purified hCG has a molecular weight of 1.5x10(5). Three subunits of molecular weights 11,000, 17,000 and 31,000 were obtained after treatment with sodium dodecyl sulfate and beta-mercaptoethanol. Amino acid analysis indicates a high content of Asp. and Glu., but a low content of Pro. Carbohydrate content is only 15.2%, and the amount of amino sugars and sialic acid are also comparatively low. The biologic activity of the purified hCG's of chorionic tissues is much lower than that from urine.

Amino Acids↗
PubMed1980

Purification of phospholipase B from Penicillium notatum by hydrophobic chromatography on palmitoyl cellulose.

Phospholipase B (lysolecithin acyl-hydrolase, EC 3.1.1.5) from the mycelia of Penicillium notatum (Institute for Fermentation, Osaka, Japan; No.4640) was adsorbed from a crude solution to palmitoyl cellulose. Adsorption was efficient at pH 4 at low ionic strength (10 mM buffer), and at pH 4-9 at higher ionic strength (1-2M NaCl in 10 mM buffer). The adsorbed enzyme was eluted from the cellulose with a suitable detergent, such as Adekatol SO-120, Triton X-100, or deoxycholate. As an application of this procedure, the enzyme was purified from an extract of the mycelia by column chromatography on a palmitoylated textile (palmitoylated gauze) with an overall recovery of 59% and a 38-fold increase in specific activity. By subsequent column chromatographies on Amberlite XAD-2, Sephadex G-100 and G-150, and DEAE-Sephadex A-50, the enzyme was purified about 4,000-fold to a nearly homogeneous state from a mycelium extract with an overall recovery of 37%.

Chromatography, Affinity↗
PubMed1979

Purification of Streptomyces chromofuscus phospholipase D by hydrophobic affinity chromatography on palmitoyl cellulose.

Phospholipase D [phosphatidylcholine cholinehydrolase, EC 3.1.4.4] excreted from Streptomyces chromofuscus was purified from the culture supernatant by precipitation with acetone and column chromatographies on palmitoylated gauze (Pal-G), DEAE-cellulose, and Sephadex G-150 with an overall recovery of 46% and 1000-fold increase in specific activity. The purified enzyme preparation showed a single band on sodium dodecyl sulfate (SDS) polyacrylamide disc gel electrophoresis. The enzyme had a molecular weight of about 50,000 by gel filtration on Sephadex G-150 or about 57,000 by SDS-polyacrylamide disc gel electrophoresis and an isoelectric point (pI) of pH 5.1 on isoelectric focusing. The enzyme hydrolyses lecithin, lysolecithin, sphingomyelin, and cephalin; the relative reaction velocities and Km's for choline-phospholipids were 87% and 1.43 mM for lecithin, 100% and 1.67 mM for lysolecithin, and 22% and 0.56 mM for sphingomyelin. The enzymatic reaction was optimal at pH 8, and its velocity was appreciably increased by either detergent (Triton X-100, deoxycholate), Ca2+ or both detergent and Ca2+. Diethyl ether stimulated the enzymatic activity by 30%; SDS and EDTA inhibited the activity. Bovine serum albumin, Triton X-100, and lipids (lecithin, lysolecithin, phosphatidic acid, lysophosphatidic acid, palmitic acid, and oleic acid) inhibited adsorption of the purified enzyme onto palmitoyl cellulose (Pal-C) and affected both the enzyme activity and stability: albumin and Triton X-100 increased the activity and enhanced the heat-stability; lysophospholipids decreased the activity but other lipids increased the activity; all the lipids lowered the heat-stability. The enzyme adsorbed on Pal-C was active, although its activity was about one-ninth of that of free enzyme, and was protected from heat-inactivation. Thus this enzyme appears to possess a hydrophobic site distinct from its catalytic site and to be adsorbed onto Pal-C through the hydrophobic site. Albumin, Triton X-100, and lipids seem to bind to the hydrophobic site and to have an appreciable effect on the enzyme activity and stability.

Cations, Divalent↗