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Biomedical subjects

S I Sytnik

Publications and source records attributed to S I Sytnik.

At least 19 recordsLinked to original sources

[The spatial structure and hierarchy of the ecological groups in the skin microbiocenosis of the breasts].

In 120 nulligravidae, 175 pregnant women and 280 puerperants the skin microbiocenosis of mammary glands was studied. Its horizontal structure, the types of the distribution of different ecological groups over the surface of the biotope and their hierarchy, as well as the diversity of species at different anatomical areas, were described. The study showed that the representatives of resident flora were characterized by group distribution, while for transitory flora variations from occasional distribution in nulligravidae to group distribution in nursing mothers were noted. The most pronounced changes in hierarchy were observed in puerperants. In nursing mothers a significant increase in the diversity of species at different anatomical areas were also disclosed.

Adolescent

[Micrococci in the skin microbiocenosis of the breasts. Ecologic characteristics of the colony].

The ecological characteristics of the micrococcal community on the skin of the mammary glands of nulligravidae, pregnant and puerperant women have been studied. The species composition of the community, the population size and occurrence of each species, its participation in the dominant structure of the community have been determined. The comparative evaluation of its types has been made. A decrease in the dominating role of M. luteus in pregnant and puerperant women has been established. The tendency towards the normalization of the structure of the community in the postnatal period has been established.

Adolescent

[The use of bifidumbacterin in the prevention of mastitis].

The efficiency of bifidumbacterin was studied in 160 parturients and 160 newborn for the prophylaxis of postpartum lactation mastitis and purulent-septic diseases in the infants 6 months after discharge from the hospital. The incidence of mastitis reduced from 6.88 to 1.25% while the morbidity in the newborn reduced three times. The agent does not inhibit the residence microflora of the breasts, favours early development of bifidoflora of the intestine in the new-born.

Adolescent

[Antibiotic sensitivity of Staphylococci populating the breast skin of nursing women].

Sensitivity to penicillins, tetracycline, erythromycin and lincomycin was tested in 1513 staphylococcal strains isolated from the skin of various anatomic areas of the breast of nursing mothers. It was shown that 82.9, 78.4 and 33.9 per cent of the isolated cultures were highly sensitive to lincomycin, erythromycin and methicillin respectively. Sensitivity to tetracycline and benzylpenicillin was detected in 33.1 and 19.8 per cent of the cultures respectively. The study of the resistance spectra of 1343 strains resistant to certain antibiotics revealed that 498 cultures (37.1 per cent) were polyresistant. None of the tested antibiotics could be used for selective decontamination of the breast skin with respect to S. aureus in prophylaxis of lactic mastitis.

Adolescent

[Ecological approach to the assessment of skin microflora].

Possible use of quantitative criteria for estimation of cenoses in skin microflora was studied. The population measures and indices for description of ecosystems were differentiated. A rational order for application of quantitative and nominal characteristics depending on the study aim is proposed and illustrated by an example of investigation of breast skin microflora in pregnant and feeding women. The spheres of the criteria application are discussed. Expediency of the criteria use in clinical microbiology, hygiene, toxicology and cosmetology is grounded.

Breast

[Population characteristics and biological properties of micrococci colonizing the skin of the breasts].

As shown in this investigation, the population characteristics of micrococci inhabiting the skin of the mammary glands make it possible to regard these microorganisms as the permanent members of microbiocenosis. Their proportion among all isolated strains was, respectively, 27.2% and 27.7% for nulliparous and pregnant women and their occurrence, 96.7% and 92.1%. The largest population of micrococci was registered at the nipple area of both left (934-1,956 colony-forming units per sq. cm) and right (1,092-2,134 colony-forming units per sq. cm) breasts. A decrease in the number of micrococci was observed at the above-mentioned areas during pregnancy, which was accompanied by changes in the specific and antagonistic profiles of the association. This occurred supposedly under the necessity of preserving its dynamic stability.

Adolescent

[Antibiotic sensitivity of staphylococci isolated from the surface of the skin of the breasts in pregnant women].

Nine hundred and fourty coagulase-positive and coagulase-negative strains of staphylococci isolated from the skin surface of the mammary glands of 94 pregnant women were tested by the disc agar diffusion method for their sensitivity to five antibiotics. The highest number of the isolates were sensitive to erythromycin and lincomycin (87.7 and 89.7 per cent, respectively). The highest number of moderately resistant strains were detected with respect to methicillin. 19 out of 42 cultures of Staph. aureus were resistant to benzylpenicillin and 24 cultures were resistant to tetracycline. Among staphylococci 130 strains or 13.8 per cent were polyresistant.

Adolescent

[Staphylococci in skin microbiocenosis of the breasts in nursing women].

The levels of the bacterial contamination of the nipple, the areola and the surrounding skin, the occurrence and species composition of staphylococci in 120 nursing women on days 4-5 after parturition have been studied. S. aureus contaminate the surface of the nipple and the areola in 75% of the examined women, and in 57.5% of these women the massive contamination of the above-mentioned areas (greater than or equal to 10(3) colony-forming units per sq. cm) is observed. In 80% of puerperae the occurrence of S. epidermidis on the nipple, the areola and the surrounding skin has proved to be practically the same. The population of S. aureus colonizing the mammary glands consists mainly of hospital strains; of these, 75.97% belong to phage type 75.

Adolescent

[Antibiotic sensitivity of Staphylococci representative of the normal skin flora of the breasts of healthy women].

Four hundred and forty seven staphylococcal strains isolated from various anatomical areas of the mammary gland skin of healthy women were studied with respect to their sensitivity to benzylpenicillin, methicillin, tetracycline, erythromycin and lincomycin. It was shown that 87.5, 85 and 81.4 per cent of the isolates preserved their high sensitivity to lincomycin, methicillin and erythromycin respectively. Sensitivity to tetracycline and benzylpenicillin was observed in 49.9 and 57.7 per cent of the isolates respectively. No resistant forms were detected among S. intermedius and S. hyicus. The strains of S. aureus were resistant only to tetracycline. 56.52 and 39.61 per cent of the S. epidermidis strains amounting to 46.3 per cent in the species structure of Staphylococcus were resistant to tetracycline and benzylpenicillin respectively. No cultures of S. epidermidis resistant to methicillin were isolated. Among 263 strains resistant to separate antibiotics 42 (16 per cent) were polyresistant.

Adult

[Staphylococci in the skin microbiocenosis of the breasts in pregnant women].

The microflora of the mammary glands in the area of the nipple, the areola and the adjacent skin was studied by the methods of washings and impression. 120 nonpregnant women and 164 pregnant women were examined. The pregnant women showed a higher level of the contamination of the above-mentioned sites. The highest density of bacterial population was detected in the area of the nipple and the lowest density, on the skin surrounding the areola. Coagulase-negative staphylococci proved to be the most numerous organisms among all bacterial population found on the skin of the mammary glands of pregnant women. Of these staphylococci, S. epidermidis was most frequently isolated, its isolation rate being higher in the pregnant women than in the nonpregnant ones.

Adolescent

[Staphylococci in skin microbiocenosis of the breasts in healthy women].

The autoflora of different anatomical regions of the mammary glands in 120 healthy nulliparous women aged 18-24 years was studied by P. Williamson and A. M. Kligler's methods of smears and washings. From the nipple, the areola, and the adjacent region of the skin 2,248 strains of anaerobic microorganisms were isolated; of these, 63.83% were staphylococci and micrococci, 6.01% were streptococci, 1.91% were Neisseria, 17.79% were Corynebacterium, 3.87% were bacilli, 2.8% were enterobacteria, and 3.79% were fungi. Coagulase-positive staphylococci occurred in 1.56% of cases. Out of 11 coagulase-negative species of this genus, S. epidermidis occurred most frequently on the skin of the mammary glands. The nipple was found to have the highest bacterial contamination (0.55 X 10(6) +/- 0.7 X 10(5) cells/sq. cm for the right mamma and 0.59 X 10(6) +/- 0.7 X 10(5) cells/sq. cm for the left mamma) and the skin adjacent the areola, the lowest bacterial contamination (0.14 X 10(4) +/- 0.2 X 10(3) cells/sq. cm for the right mamma and 0.25 X 10(4) +/- 0.3 X 10(3) cells/sq. cm for the left mamma). P. Williams and A. M. Kligman's method of washings, more accurate and informative, was found to be preferable for the study of the quantitative characteristics of the dermal microbiocenosis of the mammary glands.

Adolescent