Enduracidin, a new antibiotic. V. Structures of new basic amino acids, enduracididine and alloenduracididine.
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Amino Acids↗
Biomedical subjects
S Horii
Publications and source records attributed to S Horii.
Inhibitory effect of some nucleosides on the growth of various human viruses in tissue culture.
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Herpesviridae↗
Comparison of resistive index versus pulsatility index in assessing the benign etiology of adnexal masses.
Retrospective analysis of pelvic sonograms with colorguided spectral Doppler evaluation of 189 adnexal masses yielded four malignancies, 20 benign neoplasms, 32 cysts, and 14 endometriomas. Fifty-eight masses were presumed benign on additional imaging. Pulsatility index (PI) < 1.0 was seen in 70% neoplasms, 63% benign cysts and 50% endometriomas. Thirteen percent of benign cysts and no neoplasms had resistive index (RI) < 0.4. Fifty percent malignancies had PI < 1.0. None had RI < 0.4. Both thresholds lack sufficient sensitivity and specificity for distinguishing benign and malignant lesions.
Adolescent↗
Rapid determination of nosiheptide in meat and egg by liquid chromatography with fluorescence detection.
A procedure was developed to determine nosiheptide residues in marketed meat and egg. Acetonitrile was used for the extraction, and the extract was partitioned with hexane to remove fat. The lower layer was reconstructed and quantitated by liquid chromatography using fluorescence detection at 357 nm excitation and 500 nm emission. The mobile phase consisted of 0.025% phosphoric acid-acetonitrile (50 + 50, v/v). Recoveries of nosiheptide from fortified samples ranged from 91.3 to 95.2% for swine muscle, 88.6 to 92.7% for chicken muscle, and 86.3 to 86.8% for egg. The method was used to monitor swine and chicken muscle and egg (20 samples each) in the market. Nosiheptide was not determined in all 60 samples.
Acetonitriles↗
Determination of pyrimethamine in animal tissue and egg by liquid chromatography with fluorescence detection.
A sensitive and selective liquid chromatographic (LC) assay was developed to determine the concentration of pyrimethamine in animal tissue and egg by fluorescent derivative. Animal samples were extracted with acetonitrile, centrifuged, and purified by hexane. Fluorescent derivatization was performed by reacting pyrimethamine with chloroacetaldehyde and subjected to LC with fluorescence detection (excitation wavelength 300 nm, emission wavelength 420 nm). The limit of detection was 10 ng/g (10 ppb) and the standard calibration curve was linear in the range of 1-100 ppb (0.01-1 ng/10 microL). Recoveries from samples fortified at levels of 0.1 and 1 ppm (microg/g) were 61.0-77.4 and 65.5-81.2%, respectively. The method was applied to the monitoring of marketed samples. Pyrimethamine was not determined in any of the 70 samples: 20 swine muscle; 20 chicken muscle; 10 chicken liver; and 20 egg.
Animals↗
Liquid chromatographic determination of three sulfonamides in animal tissue and egg.
Sulfonamides are widely used as a feed additive in animal production in Japan. The present paper is a determination of 3 sulfonamides: sulfamethazine (SMZ), sulfamonomethoxine [SMX, 4-amino-N-(3-methoxypyrazinyl)-benzenesulfonamide], and sulfadimethoxine (SDX) in animal tissue and egg by liquid chromatography (LC). Tissues were extracted with acetonitrile and fat was removed by liquid/liquid partition. The sulfonamides were purified by an ODS cartridge column; then each compound was separated by an ODS LC column and detected at 268 nm. Quantification levels were 0.02 ppm for SMZ and SMX, and 0.04 ppm for SDX; detection limits were 0.01 ppm for SMZ and SMX, and 0.02 ppm for SDX. Calibration curves were linear between 2 and 40 ng for SMZ and SMX, and between 4 and 80 ng for SDX. Recoveries from muscle and egg samples spiked with 1-2 micrograms/10 g were 81-98%.
Animals↗
Posttraumatic syrinx formation: experimental study.
An experimental study was performed to examine posttraumatic spinal cord cavitation in an animal model by evaluating immediate and delayed computed tomographic (CT) scans obtained after administration of intrathecal contrast material. Four cats underwent midthoracic laminectomy and spinal cord contusion using a standard 400 g-cm model. All animals were studied by CT with intrathecal contrast enhancement before and 4-5 days, 3-4 weeks, and 7-13 weeks after experimental cord contusion. Either metrizamide or iopamidol was used as the contrast agent. Two of the four cats had CT and pathologic evidence of cord cavitation at the site of injury. Another animal had uptake of contrast material into the spinal cord without pathologic evidence of cyst formation, which was believed to represent malacic change. The fourth animal had a normal-appearing cord by both CT and pathologic criteria. Animals that received metrizamide after cord contusion had generalized myoclonic seizures. This did not occur when iopamidol was administered.
Animals↗