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Biomedical subjects

S Hara

Publications and source records attributed to S Hara.

At least 397 records · Page 22Linked to original sources
PubMed1990

Strategies for asymptomatic microscopic hematuria: a prospective study of 1,034 patients.

To establish strategies for treatment of asymptomatic microscopic hematuria we conducted a prospective study of 1,034 patients with this disease. The patients were examined by cystoscopy, urine cytology, abdominal ultrasound and excretory urography. On initial examination 30 highly significant lesions, including 24 cases of urological malignancies, 195 moderately significant lesions and 246 insignificant lesions were detected. In the remaining 563 patients no underlying lesion could be found. Of the 246 patients with insignificant lesions and 563 with unexplained asymptomatic microscopic hematuria followup was done in 421 at 6-month intervals for more than 1 year. A diagnosis became clear within 3 years in 22 patients, including 3 cases of bladder carcinoma and 1 of prostatic carcinoma.

Aged↗
PubMed1990

Proteinaceous inhibitors of phospholipase A2 purified from inflammatory sites in rats.

We have purified two phospholipase A2 inhibitory proteins (37 and 33 kDa) from peritoneal fluid of dexamethasone-treated rats. The extracellular phospholipase A2 found in inflammatory sites differed from the exocrine phospholipase A2 in susceptibility to these endogenous inhibitors; both proteins inhibited the activity of the extracellular phospholipase A2 purified from sites of inflammation but did not affect appreciably the activity of either porcine pancreatic or Naja naja venom phospholipase A2. The amino acid sequence of the NH2-terminal portion of the purified proteins did not resemble that of lipocortins so far reported, but it was almost identical to that of parts of human or mouse complement component C3. These findings may indicate that degraded products of C3 are involved in the regulation of activity of a class of mammalian phospholipase A2.

Amino Acid Sequence↗
PubMed1990

Amino acid sequence of glutathione S-transferase b from guinea pig liver.

The amino acid sequence of glutathione S-transferase b (GST b) from guinea pig liver was determined by conventional methods. GST b was composed of two identical subunits, each with 217 amino acid residues. As GSTs are generally classified into three classes, alpha, mu, and pi, GST b belonged to class mu and the amino acid sequence of GST b showed about 80% homology with that of rat GST Yb.

Amino Acid Sequence↗
PubMed1990

Pattern dystrophy of the retinal pigment epithelium.

The authors describe a family in which one member had pattern dystrophy of the retinal pigment epithelium. The proband also had neovascular membranes which resulted in decreased visual acuity. Two other family members had abnormal electro-oculographic findings, but a clinical normal-appearing fundus that could represent an early stage of the disease. The incidence of this condition in this family indicates autosomal dominance as the inheritance pattern of this dystrophy.

Adult↗
PubMed1990

[A clinico-pathological study of small renal cell carcinoma].

Between 1960 and 1989, 50 patients with small renal cell carcinoma measuring below 3 cm in diameter were examined. Among them, 31 patients were nephrectomized, but in 19 patients, the renal cell carcinoma was found first at autopsy. The average tumor diameter of the unrecognized ones was smaller than that of nephrectomized patients with the minimum of 0.4 cm. Forty-one patients had clear cell carcinoma, 9 granular cell carcinoma, 40 grade 1 tumor, and 10 grade 2 tumor. The grade 3 tumor was not found in our study. Three out of the 50 patients had distant metastasis at the time of operation or autopsy. Six patients had microscopic tumor thrombi just close to the tumor pseudo-capsule. Two patients had apparent tumor thrombi in the main renal vein. The smallest tumor that showed microscopic tumor thrombi measured 1.5 cm in diameter. The incidence of tumor thrombi was correlated with tumor diameter. The step sections by 4 mm thick of nephrectomized specimens were examined on 16 patients. The daughter tumor was found in two patients. The tumor thrombi had close relationships with the presence of distant metastasis (p less than 0.001) and/or the daughter tumor (p less than 0.01). In conclusion, small renal carcinoma measuring less than 1.5 cm in diameter was considered to be treated by conservative surgery.

Adult↗
PubMed1990

Construction of shuttle vector plasmid between Clostridium acetobutylicum and Escherichia coli.

A high copy number plasmid pCS86 (3.0 kb) was isolated from Clostridium acetobutylicum strain No. 86. For developing the vector plasmid for gene cloning of saccharolytic clostridia, two hybrid plasmids were constructed by joining pCS86 to the E. coli promoter probe vector pKK232-8 carrying the promoterless gene of chloramphenicol acetyltransferase (CAT). One of the hybrid plasmids designated as pTY10 replicated and expressed the CAT gene in E. coli, and transformed a plasmid-free strain, C. acetobutylicum strain No. 220, as a shuttle vector. This is the first shuttle vector constructed from C. acetobutylicum and E. coli plasmids. Reciprocal transformation was possible between E. coli and C. acetobutylicum, though deletion occurred in the sequence originated from pKK232-8. A deleted plasmid pTY101 only replicated in Clostridium, though it showed a high copy number.

Chloramphenicol O-Acetyltransferase↗
PubMed1990

Cloning and nucleotide sequence of the KHR killer gene of Saccharomyces cerevisiae.

The KHR gene cloned from a genomic library was on 4.7-kbp DNA fragment and was inserted into YCpG11 vector (KHR-YCp) and YEp vector (KHP-YEp). Transformants with KHR-YEp could secrete 3-4 times as much killer toxin into the media as the donor strain. The complete nucleotide sequence of the KHR gene was analyzed. It was found that the KHR gene consisted of 888 bp. It was suggested that this protein was processed before being secreted into the media, because its molecular mass presumed from the nucleotide sequence was larger than that of the mature killer toxin.

Amino Acid Sequence↗
PubMed1990

Chromosomal transformation in Saccharomyces cerevisiae with DNA isolated by pulse field gel electrophoresis.

We isolated and purified yeast chromosome DNA molecules using pulse field gel electrophoresis (PFG). The isolated DNA had nearly the same size as the native chromosomal DNA on PFG. We could directly transform Saccharomyces cerevisiae yeasts with it, and obtain transformants that were selected by complementation of several markers. They had new chromosome DNA bands observed on PFG. The new chromosome was very stable during mitosis and mating processes, and each of the three homologous chromosomes in the derivative zygotes of transformants was separated equally in daughter cells.

DNA, Fungal↗
PubMed1990

Molecular cloning of the glucoamylase gene of Aspergillus shirousami and its expression in Aspergillus oryzae.

The glucoamylase enzyme (GAase) gene from Aspergillus shirousami was cloned and sequenced from genomic and cDNA libraries. The genomic gene was located in the 5.4 kb EcoRI fragment. The deduced amino acid sequence of GAase contained 639 amino acid residues with a relative molecular mass of approximately 68,000 daltons (non-glycosylated form). The genomic gene of A. shirousami GAase was introduced into Aspergillus oryzae. These transformants had increased GAase and raw starch degradation (RSD) activity in culture media and in rice-koji extracts. Analysis by Southern, Northern, SDS-PAGE, and Western blot techniques confirmed the foreign gene was correctly transcribed, translated, and expressed in A. oryzae.

Amino Acid Sequence↗
PubMed1990

[Clinical study of UFT chemotherapy in renal cancer. Kyushu University Cooperative Study Group of Renal Cancer].

Clinical effects of UFT chemotherapy for renal cancer were evaluated by 19 collaborating hospitals. UFT (300-600 mg/day) was administered for more than 3 months. Of the 30 patients entered in this study, 21 were evaluable for the antitumor effects of the drug. Of the 21 evaluable patients, complete response (CR) was obtained in 2 patients, partial response (PR) in 1, no change (NC) in 7, progressive disease (PD) in 11, respectively. The response rate was 0% in patients with primary lesion and 21.4% in nephrectomized patients with metastatic lesion. Responses were observed in the metastases of lung, pleura and mediastinal lymph node. The main side effects in 27 patients were gastrointestinal symptoms. No significant abnormality was noted on blood laboratory data. In one patient the drug was discontinued within 3 months because of gastrointestinal symptoms. These results suggested that UFT chemotherapy for advanced renal cancer was clinically effective.

Adult↗
PubMed1990

[Results of pulmonary angioplastic operations for cases of bronchogenic carcinoma].

Pulmonary artery reconstruction for bronchogenic carcinoma was performed in 36 patients (5.8%) in our department between 1955 and 1988 for the purpose of preservation of pulmonary function. Twenty-six patients had squamous cell carcinoma (72.2%) and 24 had Stage IIIA. The operative procedures were sleeve resection of the main pulmonary artery (P.A.) and end-to-end anastomosis in 20 cases, wedge resection of P.A. and transverse suture in 14 and window type resection of P.A. and patch closure in 2. In 28 patients bronchoplastic procedures were combined. Three patients (8.3%) died within 30 days after operation. Postoperative complications were relatively high in the patients with combined operations. The 5-year survival rates were 45.7%. Postoperative pulmonary functions were evaluated with spirometric examinations and pulmonary scintigrams. There were no differences in postoperative pulmonary functions between pulmonary angioplastic procedures and simple lobectomies.(ABSTRACT TRUNCATED AT 250 WORDS)

Adenocarcinoma↗
PubMed1990

Immunohistochemical localization of cathepsin D in ocular tissues.

Cathepsin D has been believed to play an important role in the catabolism of protein in various tissues. In retinal pigment epithelium, cathepsin D degrades rod outer segments and rhodopsin into glycopeptides. To our knowledge, no reports have described the immunohistochemical localization of cathepsin D in whole ocular tissues. We investigated the reaction of bovine, rat, and human eyes with a polyclonal antibody to cathepsin D from bovine spleen. Cathepsin D immunoreactivity was observed in the cytoplasm of the following cells: epithelium and endothelium of the cornea; keratocytes; pigmented and nonpigmented epithelium of the ciliary body; epithelium and cortex of the lens; epithelium and sphincter and dilator muscles of the iris; Müller cells; ganglion cells and pigment epithelium of the retina; and endothelium of various vessels. Positively stained ocular tissues were believed to have a high activity of protein catabolism. Since cathepsin D was closely associated with phagosomes in retinal pigment epithelium, we concluded that cathepsin D probably contributes to the physiologic degradation of rod outer segments.

Adult↗
PubMed1990

[Results of re-thoracotomy for the management of early postoperative complications after bronchoplastic operations].

We reviewed cases of re-thoracotomy performed for early complications after bronchoplastic procedures. One hundred and sixteen bronchoplasties were performed in our department over 20 years. The diseases for which bronchoplasty was undertaken were lung cancer in 102 patients (87.9%), tuberculous stenosis of the bronchus in eight, esophageal cancer in three, and trauma in three. The most frequent postoperative complication was difficulty of expectoration and atelectasis, which generally improved with conservative treatment. Re-thoracotomy was performed for early postoperative intrathoracic complications on 11 patients. The reasons for re-thoracotomy were bronchial anastomotic dehiscence in five cases, obstruction of bronchial anastomosis in two, atelectasis in two, and occlusion of anastomosis of pulmonary arterial angioplasty in two cases. All except two underwent re-thoracotomy within two weeks of the first operations. The operative procedures performed were completion pneumonectomy in six cases, re-bronchoplasty in three, suture of anastomotic dehiscence in two, patch closure of pericardial defect with aspiration of secretions in the atelectatic lobe in one, and partial decortication with suture closure of the alveolar fistula in one. Pedicled omental wrapping was applied to two patients with re-bronchoplasty and one with completion pneumonectomy. Post-operative complications after re-thoracotomy were anastomotic insufficiency in two cases, bronchopleural fistula in two, and pneumonia in one. Two patients underwent a third thoracotomy. There was no anastomotic dehiscence or bronchopleural fistula in the patients with pedicled omental wrapping. One patient died due to bronchopleural fistula within 30 days of re-thoracotomy. Six patients died of recurrence or pneumonia from 39 days to one year after re-thoracotomy.(ABSTRACT TRUNCATED AT 250 WORDS)

Bronchi↗
PubMed1990

[Metastatic lung tumor of a thyroid cancer origin detected by a periodic health examination--a case of a complete response].

Reports is the case of a 21-year-old student, who received a medical examination at our Department for an abnormal chest shadow that had been detected during a periodic health examination at his university. No special subjective symptoms such as a cough and/or sputa, pyrexia, pectoralgia, and dyspnea were noted. A thyroid tumor was palpable and a 99mTc thyroid scintigram and a 201Tl thyroid tumor scintigram gave cause to suspect thyroid cancer and a metastatic lung tumor. A total thyroidectomy and a right modified neck dissection revealed a tumor (histologically, a papillary cancer), 3.5 cm in diameter, that mainly involved the right lobe. It was found that 131I-100 mCi internal irradiation was very effective for the metastatic lung tumor, and no abnormal shadow was subsequently revealed by chest X-ray. Now, 6 years later, he is alive with no manifestations of a local relapse nor any abnormal chest X-ray findings.

Adult↗
PubMed1990

[Preoperative gastric acid secretion of gastric cancer patients and their prognosis].

This study was performed to evaluate how preoperative gastric acid secretion in gastric cancer patients correlated their prognosis. The results are as follows. 1) The 5-year survival rates of patients with MAO = 0mEq/h, 0mEq/h less than MAO less than or equal to 7.0mEq/h and 7.0mEq/h less than MAO were 29.2%, 73.6% and 88.4%, respectively. There were significant differences among them. 2) The patients with 7.0mEq/h less than MAO had normal cellular immunity and low immunosuppressive glycoproteins in sera. These results suggest that gastric cancer patients who have a potency of normal gastric acid secretion with normal cellular immunity, or who have a potency of normal immunity with normal gastric acid secretion, have a good prognosis.

Gastric Acid↗