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Biomedical subjects

S Hao

Publications and source records attributed to S Hao.

At least 37 records · Page 2Linked to original sources
PubMed2000

[Vertical distribution and quantitative dynamics of dominant functional groups of arthropod community in rice fields and estimation of natural enemy effects].

The study showed that Lycosid (wolf spider) mainly distributed in the basal part of rice plants, not as wide as we know in past. Tetragnathid did not limited in the upper part of plants, but might translate to middle or lower part when affected by insecticide. The relationship between the vertical distributions of top and basal species was not significant. Besides the amount of natural enemies, the spatial distribution characteristics of natural enemies and brownplanthopper, and the proportion of brownplanthopper to total preys of natural enemies were the factors affecting the role of natural enemies on the population dynamics of brownplanthopper in rice fields. A model to evaluate the effect of natural enemies to brownplanthopper was put forward, which included the message of quantitative dynamics, spatial distribution and feeding characteristics of natural enemies, brownplanthopper and neutral insects.

Animals↗
PubMed2000

[An observation of the immuno-persistence after inoculating with the domestic BRD II strain rubella vaccine among infants and young children].

OBJECTIVE: To study the immuno-persistence after inoculating with the domestic BRD II strain rubella vaccine among infants and young children. METHODS: Hemagglutination inhibition (HI) antibody detection method was used to test on children at age 6 to 18 months without rubella infection or rubella vaccine immunization in Yantai city of Shandong province and were selected for the observation of the immuno-persistence against the domestic BRD II strain rubella vaccine from 1995 to 1998. RESULTS: Positive rates of HI antibody among children of 6, 7, 8, 9, 12 and 18 month-old of inoculation were 94.44%, 97.22%, 96.67%, 100.00%, 100.00%, 100.00% and 93.10%, 93.55%, 96.77%, 96.97%, 100.00%, 100.00% (P > 0.05), in one or two years respectively. The geometric mean reverse titers (GMRTs) were about 50% lower than that after 1 month after 1 year. Similar results were found that after 2 years GMRTs was about 50% lower than that after 1 year of inoculation. There was highly significant difference on GMRTs of HI antibody between infants with 6 to 7 month-olds (29.89) and young children with 8 to 18 month-olds (53.00) after 2 years of inoculation (t = 3.58, P < 0.001). CONCLUSION: The immunization schedule at the first dose for BRD II strain rubella vaccine should be started when the child is 8 month old. For the second dose, the immunization schedule used in other developed countries should be referred adopted in China.

Antibodies, Viral↗
PubMed1999

Isobolographic analysis of interaction between spinal endomorphin-1, a newly isolated endogenous opioid peptide, and lidocaine in the rat formalin test.

Endomorphin-1, a newly isolated endogenous opioid ligand, has a potential affinity with mu-opioid receptor. We investigated antinociception of intrathecal endomorphin-1 and lidocaine in the rat formalin test and examined the interaction between the two agents using isobolographic analysis. Intrathecal endomorphin-1 caused dose-dependent suppression of the formalin-induced biphasic behavioral response. Intrathecal lidocaine produced dose-dependent inhibition of phase-2 behavioral response. Isobolographic analysis confirmed that combination of intrathecal endomorphin-1 and lidocaine, given at a fixed dose ratio, produced synergistic suppression of phase-2 behavioral response. These data demonstrate that spinal endomorphin-1 synergistically interacts with local anesthetic lidocaine in producing antinociception in the formalin test.

Analgesics, Opioid↗
PubMed1999

Tropomyosin is localized in the nuclear matrix and chromosome scaffold of Physarum polycephalum.

The nuclei and chromosomes were isolated from plasmodia of Physarum polycephalum. The nuclear matrix and chromosome scaffold were obtained after the DNA and most of the proteins were extracted with DNase I and 2 M NaCl. SDS-PAGE analyses revealed that the nuclear matrix and chromosome scaffold contained a 37 kD polypeptide which is equivalent to tropomyosin in molecular weight. Immunofluorescence observations upon slide preparations labeled with anti-tropomyosin antibody showed that the nuclear matrix and chromosome scaffold emanated bright fluorescence, suggesting the presence of the antigen in them. Immunodotting results confirmed the presence of tropomyosin in the nuclear matrix and chromosome scaffold. Immunoelectron microscopic observations further demonstrated that tropomyosin was dispersively distributed in the interphase nuclei and metaphase chromosomes.

Animals↗
PubMed1999

[Direct and indirect somatic embryogenesis in Freesia refracta].

Somatic embryogenesis can be induced in tissue cultures of Freesia refracta either directly from the epidermal cells of explant, or indirectly via intervening callus. In direct pathway, somatic embryos were in contact with maternal tissue in a suspensor-like structure. In indirect pathway, the explants first proliferacted to give rise to calluses before embryoids were induced. The two sorts of calluses were defined to embryogenic callus and non-embryogenic callus according to producing of somatic embryos. An indirect somatic embryo is developed from a pre-embryogenically determined cell. This kind of somatic embryo has no suspensor structure instead of a complex with maternal tissue. Somatic embryos have their own vascular tissues, and can develop new plantlets independently.

Culture Techniques↗
PubMed1999

[Immunocytochemical identification of tropomyosin in nucleoli and nucleolar matrix of Physarum polycephalum].

Nucleoli were isolated from physarum polycephalum, and nucleolar matrix was prepared by digesting the nucleoli respectively with DNase 1, 0.25 mol/L (NH4)2SO4 and 2 mol/L NaCl to remove DNA and most proteins. SDS-PAGE analysis indicated that there were about 20 polypeptides in nucleolar matrix component, including the 37 kD polypeptide which was similar to tropomyosin in molecular weight. The result of indirect immunofluorescence treated with anti-tropomyosin antibody and sheep anti-rabbit IgG antibody labelled with FITC showed that bright fluorescence was observed in the nucleoli and nucleolar matrix, but no bright fluorescence in the controls. Indirect Immunoblotting detection further verified that tropomyosin existed in nucleolar matrix. Protein A-colloidal gold immunoelectron microscopic study showed that there were many gold particles in the specimens labelled with tropomyosin antibody, and there were few gold particles found in the controls. Tropomyosin distributed dispersedly in nucleoli.

Animals↗
PubMed1998

Sevoflurane suppresses behavioral response in the rat formalin test: combination with intrathecal lidocaine produced profound suppression of the response.

We investigated the effects of intrathecal (i.t.) lidocaine, inhalation sevoflurane, and a combination of i.t. lidocaine and sevoflurane on the formalin test in rats. Group 1 (control) received i.t. saline 10 microl. Groups 2 and 3 received i.t. lidocaine 200 microg and 400 microg, respectively. Groups 4-6 received 1.2%, 2.4% and 3.6% sevoflurane, respectively. Interaction of drugs was analyzed using a dose addition model. Group 7 received i.t. lidocaine 200 microg and 1.2% sevoflurane. The biphasic behavioral activity of the hindpaw of rats was observed. This study showed that i.t. lidocaine or inhalation sevoflurane before formalin injection, significantly suppressed the behavioral activity of the hindpaw of rats, and that this suppression was significantly potentiated by the co-administration of i.t. lidocaine and inhalation sevoflurane.

Anesthetics, Inhalation↗
PubMed1998

Naltrexone, but not atropine or yohimbine, antagonizes suppression of formalin-induced spinal sensitization by intrathecal nociceptin.

We investigated the effects of spinal nociceptin on formalin-induced spinal sensitization and examined the role of the opioidergic, alpha 2-adrenergic and muscarinic cholinergic receptors in the nociceptin-produced suppression of spinal sensitization. The results demonstrated that spinal nociceptin suppressed the formalin-induced spinal sensitization in a dose-dependent manner (1, 5 and 10 nmol). The inhibitory effect of 10 nmol of nociceptin on spinal sensitization, was readily antagonized by naltrexone, but not by atropine or yohimbine. Each of the antagonists, naltrexone, atropine or yohimbine, alone had no effect on the formalin-induced spinal sensitization. Our results show that spinal nociceptin elicits dose-dependent, naltrexone-reversible suppression of spinal sensitization evoked by injection of formalin.

Animals↗
PubMed1996

[Ultramicrostructural changes of articular cartilage, subchondral bone and synovium in human osteoarthritis of patellofemoral joint].

Ten patients with patellofemoral joint osteoarthritis (OA) were studied by electron microscope (SEM&TEM) to identify ultramicrostructural changes in the destroyed articular cartilage, subchondral bone and synovium. According to the results obtained, the author suggested that (1) Articular cartilage, subchondral bone and synovium in patellofemoral joint OA were noted to be in various degree of ultramicrostructural changes; (2) The cartilage degeneration in lateral compartment of patellofemoral joint were much more severe than that in medial compartment; (3) The subchondral bone had a strong tendency to undergo selerosis in the form of thicking and confluence of the trabeculae to form massive block of bone, which were not parallel to alteration of cartilage, and the rules of alteration were related to the character of loading; (4) The repair tissues of I-II grade degenerative cartilage were similar to normal cartilage, but those of III-IV grade degenerative cartilage were similar to fibrocartilage in many ways, because the source of repair tissues were different between them; (5) Alteration of synovium revealed only mild non-specific inflammatory changes following the changes of articular cartilage; (6) I-II grade patellofemoral joint OA was treated by conservative methods and III-IV grade OA should be treated by patellofemoral joint resurfacing.

Adult↗
PubMed1994

Reorganization and condensation of chromatin in mitotic prophase nuclei of Allium cepa.

This paper studies the process and features of chromosome construction in mitotic prophase cells of Allium cepa. The results showed that a prominent reorganization of chromatin occurred during G2--early prophase. The 250-400 nm thick compact chromatin threads in G2 nuclei began to disorganize into about 30, 100 and 220 nm chromatin fibres which constituted the loosely organized chromosome outlines in early prophase before chromosome condensation. In middle prophase, chromosome condensation was characterized by the formation of many condensed regions (aggregates of chromatin), which increased in size (1-1.5 microns) when prophase proceeded. Meanwhile, the chromatin threads that constituted and connected the condensed regions became increasingly thicker (120-250 nm). In late prophase adjacent condensed regions fused to form cylinder-shaped chromosomes. Based on these observations, we come to the conclusion that the construction of prophase chromosomes is a two-step process, that is, the reorganization and condensation of chromatin. In addition, we report the study of silver-stained, DNA- and histone-depleted prophase chromosomes, describe morphological features of the non-histone protein (NHP) residue in early, middle and late prophase chromosomes, and discuss the roles of NHPs in chromosome construction.

Allium↗
PubMed1994

Evaluating relative efficiencies of Veterans Affairs Medical Centers using data envelopment, ratio, and multiple regression analysis.

Several forms of analysis are used to evaluate the efficiency of acute care Veterans Affairs hospitals. The smaller hospitals with fewer than 100 beds and hospitals not in operation a full year have been excluded. The types of analysis used are input ratio analysis and productivity analysis of three outputs consisting of surgeries, discharges and emergency and outpatient visits. Also used is data envelopment analysis (DEA) of two subsamples segmented by whether the hospital is a member or not of the Council of Teaching Hospitals. Finally, multiple regression is used to find indicators of the relative efficiencies generated by the DEA. Results show that about one half of all hospitals are relatively inefficient. Also promising indicators of efficiency are hospital size, inpatient surgeries, outpatient surgeries, and the aggregate emergency room and outpatient visits.

Ambulatory Care↗
PubMed1994

[A primary evaluation of VAS for use in clinical experimental pain assessment].

The visual analogue scale (VAS) is a commonly used method for pain measurement abroad, though its clinical use has not been reported in our country. Fifty patients, who had received lower abdomen and lower extremities stimulation, were studied. Pain was induced by hand needle and electric needle stimulation. The data demonstrated that VAS is reliable and precise, that electric needle stimulation is more sensitive than hand needle stimulation, and that the factors of age, profession and education level affect the measured value of pain.

Adolescent↗
PubMed1991

An analysis of sex ratio of the elderly in Heilongjiang province.

The authors analyze data concerning the sex ratio among those aged 60 and over living in Heilongjiang province, China. "Data from...three population censuses and the 1987 1% sample survey of Heilongjiang Province both [indicate] that the sex ratio of the elderly population is without exception higher than the sex ratio of the whole population...." Some reasons for this differential are discussed, including migration and excess female mortality.

Adult↗
PubMed1991

[An electron microscopic study on the RNA component of synaptonemal complexes in spermatocytes of Mus musculus].

The ultrastructural and cytochemical features of synaptonemal complexes (SC) in sections of spermatocytes of Mus musculus were studied under electron microscope. In specimens stained with uranyl acetate and lead citrate the SC was found consisting of three main elements. the lateral element (LE), the central element (CE) and the transverse filament (L-C filament). When stained with the Bernhard's technique, the SC was recognized as a contrasted, tripartite structure which was usually located in the bleached area occupied by the condensed chromatin and composed of highly electron-dense LEs and medium electron-dense CE and L-C filaments. The SC and the LE, stained either by uranyl acetate-lead citrate or by the Bernhard's technique, always showed diameters of about 210 nm and 60 nm, respectively. The results suggest that RNA may be an important component of the SC.

Animals↗