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Biomedical subjects

S Handa

Publications and source records attributed to S Handa.

At least 361 records · Page 20Linked to original sources
PubMed1983

Pattern of myocardial hypertrophy as a possible determinant of abnormal Q waves in hypertrophic cardiomyopathy.

Echocardiographic and electrocardiographic findings in 74 adults with hypertrophic cardiomyopathy (HCM) were analyzed to identify the pattern of myocardial hypertrophy as a possible determinant of abnormal Q waves. The pattern of septal hypertrophy along the left ventricular long axis was divided into 3 types based on the site of maximum septal hypertrophy: basal, diffuse and apical types. Abnormal Q waves defined by the revised Minnesota Codes (either I-I, I-II or I-III) were noted in 31 cases (42%). The total incidence of abnormal Q waves in the basal type (15/26, 58%) and in the diffuse type (12/22, 55%) was significantly higher (p less than 0.001 and p less than 0.01, respectively) than that in the apical type (4/26, 15%). The abnormal Q waves defined by the strict criteria of Code I-I were significantly more prevalent (p less than 0.05) in the basal type than in the diffuse type, although there was no significant difference in the total incidence of abnormal Q waves between these 2 groups. Thirty-six patients with an extension of hypertrophy to the right ventricle (RVH) had a significantly higher incidence of abnormal Q waves than 22 patients without RVH (56% vs 27%, p less than 0.05). Furthermore, close relationships of RVH to the location of abnormal Q waves were documented. In conclusion, the abnormal Q waves in HCM may be related to the pattern of septal hypertrophy along the left ventricular long axis and to RVH.

Adult↗
PubMed1983

[Multiserial ECG-gated computed tomographic method: Evaluation of left ventricular volumes, wall thickness and wall motion].

The purpose of this study was to investigate the potential usefulness of multiserial ECG-gated computed tomography (CT) (GE CT/T 8800) for a quantitative assessment of left ventricular volume, wall thickness and wall motion in 15 patients with coronary heart disease. In order to obtain ideal left ventricular short-axis images, the gantry was tilted to the cephalad 15 degree with patients on the right lateral position. Five ECG-gated scans (5.6 sec for one scan) were performed in each patient with intravenous injection of contrast material (Urografin 76). Left ventricular volumes were computed by a modified Simpson's rule and segmental wall motion was analyzed at three levels (base, papillary muscle and apex) by tracing the endocardial outline at end-diastole and end-systole. Left ventricular systolic and diastolic volumes ( LVSV , LVDV ) had a good correlation with those measured by left ventriculography (LVG) (r = 0.75 and 0.66). Wall thickness of the interventricular septum and left ventricular posterior wall obtained by CT was correlated with those by M-mode echocardiography (r = 0.82 and 0.71). Comparative analysis of segmental wall motion by CT, two-dimensional (2 D) echocardiography and left ventriculography was done. The 2 D echocardiographic segments with normal and abnormal wall motion were accurately identified by CT, best at the papillary muscle level (91%), and less at the base (71%) and the apex (73%). Similarly, 85% of left ventriculographic segments could be detected by CT. In conclusion, ECG-gated computed tomography proved to be a useful method for evaluating left ventricular function in coronary heart disease.

Adult↗
PubMed1982

Effect of glycolipids and glycophorin on the activity of human interferon-beta and -gamma.

We have studied neutralization of the antiviral activity of human fibroblast (IFN-beta) and immune interferon (IFN-gamma) by incubation with glycolipids (including the gangliosides GM1, GM2 and GM3, as well as various other glycolipids) and with the sialoglycoprotein, glycophorin. When 100 units/ml of IFN-beta were preincubated with 30-80 microM of the gangliosides, all antiviral activity was abolished. Similarly, 120 microM of glycophorin completely reversed the antiviral activity of 100 units/ml of IFN-beta. Glycolipids containing more than two sugars also showed moderate inhibitory effects. GM2 at a concentration of 200 microM almost completely inhibited the antiviral activity of 100 units/ml of IFN-gamma, but GM1, GM3 and glycophorin had only a moderate inhibitory effect. These results suggest that the terminal N-acetylneuraminic acid (NANA) residues of gangliosides and of glycophorin play an important role in the inhibition of IFN-beta, and that they may be similarly involved in the inhibition of IFN-gamma.

Binding Sites↗
PubMed1982

Application of field desorption mass spectrometry for the analysis of sphingoglycolipids.

Simple molecular species of intact ceramide mono-, di-, tri-, tetra-, and pentasaccharides purified by reversed phase high-performance liquid chromatography (HPLC) were analyzed by field desorption mass spectrometry (FD-MS). The analysis of sphingoglycolipids without chemical derivatization by FD-MS not only provides molecular information but also significant characteristic fragments for structural determination due to the cleavage of glycosidic bonds. These ions, therefore, give information on the molecular species of sphingoglycolipids and sugar sequences of their oligosaccharides. Intact GL1a, GL2a, and an equimolar mixture of GL1a and GL2a were also analyzed by FD-MS. In the spectra, the ions, (M + H)+, (M + Na)+, and (M + H- H2O)+, were observed as high intensive ions and different molecular species ions thereafter could be identified in all spectra. The FD-MS method is particularly useful in structural studies of glycolipids from natural sources.

Chromatography, High Pressure Liquid↗
PubMed1982

Structural study on gangliosides from rat liver and erythrocytes.

Gangliosides were isolated from rat liver and erythrocytes by chromatography on columns of DEAE-Sephadex and Iatrobeads, and finally purified by preparative TLC. The chemical structures of the purified components were studied by carbohydrate analysis, methylation analysis, sialidase treatment, fatty acid analysis and direct mass spectrometry. In rat liver, gangliosides GM3, GM1, GD3, GD1a, GD1b, and GT1b were identified. Gangliosides in rat erythrocytes were characterized as GM1, fucosyl-GM1, and GD1a. Sialic acid was the N-acetyl type only and lignoceric acid was the main fatty acid in all components of rat liver and erythrocytes.

Animals↗
PubMed1982

Characteristics of cultured tomato cells after prolonged exposure to medium containing polyethylene glycol.

Cell lines of tomato (Lycopersicon esculentum Mill., cv. VFNT-Cherry) have been isolated, which are capable of growing in media containing polyethylene glycol (PEG) 6000 with water potentials as low as -15 or -22 bar. After prolonged exposure to media containing PEG, these cell populations have reduced abilities to grow in the absence of PEG. Upon resuspension in PEG-free medium, the cells swell and begin to release metabolites, including protein. Measurement by plasmometry of the osmotic potential of cells selected in medium with -22 bar water potential indicates that they maintain, at the end of the growth cycle, an osmotic potential of approximately -26 bar. This is compared to an osmotic potential of -9 bar for nonselected cells in medium without PEG, having an initial water potential of -4 bar. Thus, considerable osmotic adjustment occurs as a result of exposure to external low water potential. The results also indicate that PEG does not contribute significantly to osmotic adjustment of the cells.

Journal Article↗
PubMed1982

Growth and water relations of cultured tomato cells after adjustment to low external water potentials.

Cultured cells of tomato, Lycopersicon esculentum Mill. cv VFNT-cherry, have been selected for resistance to water stress (low water potential) imposed by the addition of polyethylene glycol to the culture medium. The ability of nonselected cells to grow in media with low water potentials changes dramatically with the age of the cells (with respect to days following inoculation) whereas there is little effect of the age of selected cells on growth over the same media water potentials. The increased resistance of selected cells has limited stability in the absence of stress, indicating that resistance is established by a slow reversible adaptive process.Increased resistance (growth) in the presence of water stress appears to result from considerable osmotic adjustment by the cells. Growth cycle-dependent changes in resistance of nonselected cells are correlated with osmotic potential changes which are associated with the normal cell growth pattern in culture. Lowered osmotic potential is maintained by selected cells throughout the entire growth cycle and may explain the growth cycle independence of growth of selected cells on polyethylene glycol-containing media. Osmotic adjustment of resistant cells at stationary phase can be as much as 40 bar. Turgor is maintained by resistant cells (as high as 21 bar) in media with low water potentials at least partly at the expense of cell expansion.

Journal Article↗
PubMed1982

Postoperative thallium-201 myocardial images. Evidence of regression of right ventricular hypertrophy in man.

Thallium-201 myocardial scintigraphic studies were performed on 24 patients with chronic right ventricular overload before and after surgical correction of haemodynamic overload. The ages of the patients ranged from 20 to 65 years (mean 39 years) at operation. The degree of right ventricular visualisation remained essentially unchanged in an early postoperative study (four to 60 days), though a decrease in right ventricular cavity size was noted in patients with right ventricular volume overload. On later follow-up (18 to 36 months), thallium uptake in the right ventricle was definitely less than before operation in all 13 patients studied at this time. Because thallium-201 radioactivity reflects myocardial blood flow and mass, our study indicates that right ventricular hypertrophy is largely reversible. Thus, thallium-201 myocardial scintigraphy can be used to assess non-invasively regression of right ventricular hypertrophy in patients with right ventricular overload.

Adult↗
PubMed1982

Evaluation of combined valvular prolapse syndrome by two-dimensional echocardiography.

The patterns of aortic and tricuspid valve motion in 50 patients with mitral valve prolapse were analyzed by wide-angle, phased-array, two-dimensional echocardiography. Twelve patients (24%) had redundant aortic leaflets bulging into the left ventricular outflow tract during diastole. Eight of 12 patients had aortic regurgitation and seven of 12 had M-mode echocardiographic evidence of aortic valve prolapse. One patient underwent mitral and aortic valve replacement, and the excised valves revealed marked myxomatous degeneration. Eight of 15 patients undergoing contrast echocardiography had tricuspid regurgitation (systolic reflux of contrast material into the inferior vena cava persisting for more than 10 beats), and prolapse in the septal leaflet of the anterior leaflet or both. A similar tricuspid valve pattern was noted in three of seven patients without tricuspid regurgitation. Tricuspid valve prolapse was identified in 20 patients (40%). Nine patients (18%) had combined prolapse of the mitral, aortic and tricuspid valves. In five patients with middiastolic high-pitched murmurs recorded along the left sternal border, tricuspid valve prolapse was demonstrated. In one of these patients, the presence of pulmonary regurgitation was confirmed by intracardiac phonocardiography. We conclude that two-dimensional echocardiography is useful for evaluating patients with combined valvular prolapse syndrome.

Adolescent↗