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Biomedical subjects

S Guo

Publications and source records attributed to S Guo.

At least 199 records · Page 11Linked to original sources

[Determination of sugar components in wild and cultured sclerotia of Grifola umbellata (pers. ex Fr.) Pilat at different ages].

Our determination indicates that in both wild and cultured sclerotia of Grifola umbellata the contents of reducing sugar decrease with the growing years; the contents of Polysaccharide peak in the second year of growth; the contents of protein increase with the growing years; the contents of fat reaches maximum in the first year of growth; in cultured sclerotia ergosterol becomes highest in contents when the plant is two years old, while in wild sclerotia it remains lowest.

Ergosterol↗

Effects of dexamethasone on pulmonary function following extubation.

Dexamethasone is often given to intubated neonates to facilitate successful extubation. To study the effects of dexamethasone on pulmonary function immediately following extubation, we conducted a randomized, blinded, placebo-controlled trial in 51 infants. All infants had been intubated for a minimum of 3 days but no more than 30 days. Mean weight at extubation was 2.41 kg in treated infants, 2.25 kg in control infants. When infants were deemed ready for extubation, dexamethasone 0.5 mg/kg/dose or an equal volume of normal saline was given in three doses 8 hours apart. The final dose was given 1 hour before extubation. Esophageal pressure, air flow integrated to tidal volume (Vt), respiratory rate, and heart rate were measured before extubation, immediately following extubation, and every 20 minutes for 80 minutes. Total pulmonary resistance (RTP), dynamic lung compliance (CL), and minute ventilation (VE) were calculated. Forty-two infants completed the study; 19 infants received dexamethasone and 23 received placebo. There was no difference between the two groups in gestational age, weight at study, or length of intubation. Vt, RTP, VE, and CL were not significantly different between the two groups over time; however, RTP increased over time in the placebo group. Heart rate was significantly lower in the dexamethasone group. We conclude that dexamethasone appears to have limited effect on pulmonary function immediately following extubation in the population studied. Further studies should evaluate the drug effect beginning at least 1 hour after extubation.

Dexamethasone↗

[The effects of dachengqi decoction, shaogan decoction, emodin and sennoside on the histamine level of intestinal mucosa in intestinally obstructed rats].

The experimental intestinal obstruction model was made by partly ligating the ileum in rat. The histamine levels of obstruction groups (8.14 +/- 2.28 micrograms/g) were obviously lower than those of control groups (P < 0.01). After administration of Dachengqi decoction, Shaogan decoction, emodin and sennoside, the levels of histamine were significantly higher than those of obstruction groups, but there were no significant differences as compared with control groups.

Animals↗

[The relation between the seed germination and seedling development of Bletilla striata and Mycena osmundicola etc. fungi].

This paper reports the effects of Mycena osmundicola etc. fungi on the seed germination and seedling growth of Bletilla striata. The results showed that B. striata seeds are easily germinated under normal conditions and belong to a special type among Orchidaceae. Fungi promote cotyledon differentiation and growth, as well as rhizoid formation of B. striata protocorms after seeds are sown with fungi. A liquid extract mixture enhanced the percentage of seed germination, and carbinol extract notably accelerated differentiation and growth of the protocorm's cotyledon after seeds were incubated with the liquid extract of Mycena osmundicola-culture products.

Agaricales↗

Analgesia produced by pituitary ACTH and dopaminergic transmission in the arcuate.

Analgesia (NAA) caused by nonacupuncture point (abdominal muscle) stimulation after lesioning the analgesia inhibitory system (AIS) or treating the subject with proglumide was abolished by hypophysectomy or adrenalectomy. The final sector of the NAA afferent pathway from the nonacupuncture point to the pituitary gland and the initial sector of the descending pain inhibitory system were found in the anterior and posterior arcuate nucleus (A-HARN and P-HARN), respectively. Analgesia caused by ACTH microinjected into the P-HARN disappeared after denervation of the A-HARN, but that caused by dopamine did not. Firing rates of P-HARN neurons were increased by nonacupuncture point simulation (NAPS) after lesion of the AIS or treatment with proglumide. The NAPS responsive neurons also responded to ultramicroinjected dopamine, but not to ultramicroinjected ACTH. Both NAA and NAPS responsive neuron activity that were abolished by hypophysectomy were restored by concurrent application of NAPS and intraperitoneal ACTH. Reduction of sodium ions due to adrenalectomy was found to abolish NAA. It was concluded that NAA production involves dopaminergic transmission in the HARN and ACTH acting presynaptically on this transmission.

Adrenal Glands↗

[Studies on the effects of fungi on the course of seed germination of Dendrobium lohohens and Dendrobium candidum].

This paper reports the results of studies on the effects of fungi isolated from the protocorms of Orchidaceae on the course of seed germination of D. lohohens and D. candidum. The experiments revealed that these fungi could promote seed germination: the seed germination rate of D. lohohens was 20%, that of D. candidum was 64%, and that of control was zero. The changes of cell ultrastructure in the course of fungus-infected seed germination of D. candidum was also studied. After hyphae of M. osmundicola penetrate the embryo from the suspensor, organelles of embryonic cells infected with fungi will disappear. When the hyphae wall is thickened and enclosed by the embryo's, the hyphae will be digested and used as the source of nutrition for seed germination. Also, the metabolism of cells without infection, but adjacent to those cells penetrated by hyphae is enhanced.

Fungi↗

Simplified limbal incision for extraocular rectus muscle surgery.

We describe a limbal incision for extraocular muscle surgery which involves only one incision to the conjunctival-Tenon's layer instead of the three separate incisions required in the standard limbal approach. Based on the 316 extraocular muscle operations in which we have used this one-snip procedure, we conclude that it is simpler and faster than the standard limbal incision, and that it provides good surgical exposure and probably less tissue damage.

Esotropia↗

[Da cheng qi tang on 45Ca content of the isolated colon smooth muscle from experimental colon obstruction rats].

Da Cheng Qi Tang (DCQT) is a classical prescription of Chinese medicine for treatment of acute intestinal obstruction. In this paper, the isolated colon smooth muscle from normal and experimental colon obstruction (CO) rats were used to study the effect of DCQT on 45Ca content. The results showed that the 45Ca content on isolated colon smooth muscle (microns/g wet tissue, mean +/- S) was 0.043 +/- 0.009 in the normal and 0.057 +/- 0.012 in those treated by DCQT respectively. The content of 45Ca in CO was higher than the normal, DCQT can reduce the content of 45Ca in CO. It is known that the higher level of intracellular CA++ is related to the formation and the development of acute intestinal obstruction. The inhibitory effect of DCQT on 45Ca content may play an important role in the treatment of acute colon obstruction.

Animals↗

[Determination of primary metabolic products of fungi promoting seed germination of Gastrodia elata Bl. and other Orchidaceae medicinal plants].

The contents of amino acids, protein, water-soluble sugar, reducing sugar and microelements in seven strains of seed-germination-promoting fungi were determined. It was found that the contents of primary products were different in these fungi. In the light of physiological characteristics of seed germination, the action mechanism of the products in promoting seed germination was discussed.

Amino Acids↗

[Effects of fungi and its liquid extract on seed germination of Dendrobium hancockii Rolf].

The experiment revealed that several species of fungi isolated from the protocorms of Orchidaceae notable promote the seed germination of Dendrobium hancockii. The seed germination rate amounts to over 20% when the seeds are incubated with fungi, but zero without incubation. Seed germination rate ranges from 40 to 70% when the incubation is affected in liquid extract of the cultural products of fungi, but the growth of protocorms and seedlings of Dendrobium hancockii is slower in liquid extract of fungi than on mycolia.

Fungi↗

A modified extracapsular cataract extraction for pediatric cataracts.

We present a modified procedure of extracapsular cataract extraction (ECCE) with a small central posterior capsulectomy for pediatric cataracts that is designed to eliminate posterior capsule opacification and to keep open the option of later secondary implantation of a posterior chamber intraocular lens. Of 76 study eyes in which the procedure was performed only three developed posterior capsule opacification. All seven control eyes that had standard ECCE with the posterior capsule left intact developed secondary membranes shortly after surgery.

Adolescent↗

Preferred postoperative alignment after congenital esotropia surgery.

A total of 117 patients with congenital esotropia who underwent muscle surgery were included in our study and were followed for a minimum of three years. Only those patients who were orthotropic or were within +/- 10 prism diopters (PD) of orthotropia six months postoperatively were included. Of the 117 patients, 101 patients (86%) remained orthotropic or within +/- 10 PD of orthotropia three years postoperatively, but 13 patients (11%) developed consecutive exotropia (greater than 10 PD). Five years after surgery, 17 of 68 patients (25%) had consecutive exotropia (greater than 10 PD). Our study demonstrates that despite satisfactory postoperative alignment, there is a steady progression towards exotropic drift over long-term follow-up. Our study also suggests that a preferred alignment shortly after congenital esotropia surgery is within 10 PD of esotropia since all of the patients who had consecutive exotropia (greater than 10 PD) were either orthotropic or exotropic (less than or equal to 10 PD) six months after surgery.

Esotropia↗

Weight and recumbent length from 1 to 12 mo of age: reference data for 1-mo increments.

Serial data from 504 infants were used to develop reference data for 1-mo increments in weight and recumbent length by fitting a family of three-parameter mathematical functions to the serial data for each individual. The percentiles for status and for increments tended to be larger for the boys than for the girls except for the lower percentiles of increments for weight after 9-10 mo and for recumbent length after 8-9 mo. These data can be used to assess growth in white or black infants whether they are breast-fed or formula-fed. These new data will allow earlier evaluation of growth velocity than the reference data for 3- and 6-mo increments that were available previously. This can lead to the earlier initiation of diagnostic procedures and treatment. The present data should be useful also in research studies.

Body Height↗

Nucleotide sequence and analysis of the N-terminal coding region of the Spodoptera-active delta-endotoxin gene of Bacillus thuringiensis aizawai 7.29.

The nucleotide sequence of a 2711bp DNA segment which contains the N-terminal coding sequence and the 5' flanking region of a crystal protein gene (bta) from Bacillus thuringiensis subsp. aizawai 7.29 has been determined. The coding region encodes an 824 amino-acid polypeptide corresponding to a carboxy-terminally truncated delta-endotoxin specifically active against the cotton leaf worm Spodoptera littoralis. Comparison of the deduced amino acid sequence of the bta gene with that of the 4.5, 5.3 and 6.6 kb classes of lepidopteran-active delta-endotoxins revealed that the Bta sequence contains a very high level of amino acid substitutions in the N-terminal part of the protoxin molecule. The substitutions are grouped in several highly variable segments separated by highly conserved regions. These conserved domains are also present in the dipteran- and coleopteran-active delta-endotoxins. The control region of the bta gene shows considerable DNA identity with the control regions of the other lepidopteran-active genes. Deletions of the 3' region of the gene were carried out and the toxic fraction of the bta delta-endotoxin was identified with the N-terminal half of the molecule.

Amino Acid Sequence↗

Reference data for head circumference and 1-month increments from 1 to 12 months of age.

Serial head circumference measurements in 462 participants in the Fels Longitudinal Study were used to develop reference data for ages 1 to 12 months. We used a three-parameter mathematical function to model the serial head circumference data for each individual and estimated head circumference at 1-month intervals. Reference data for selected percentiles of head circumference status and 1-month increments were calculated from the estimated head circumferences. These reference data are presented in tables and charts for use in testing hypotheses about group differences or to monitor changes within individuals.

Cephalometry↗