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Biomedical subjects

S Fukuchi

Publications and source records attributed to S Fukuchi.

At least 289 records · Page 16Linked to original sources
PubMed1977

[A simple method measuring plasma aldosterone by radioimmunoassay without extraction (author's transl)].

A radioimmunoassay method was developed to measure plasma aldosterone levels. Antibody was produced in rabbits by injecting aldosterone oxime coupled with bovine gamma-globulin once a month. Plasma aldosterone was measured simultaneously by two methods: the direct method without extraction and a method using paper chromatography. 125I-aldosterone was used in the first method and 3H-aldosterone in the second. The antibody had a high specificity adequate to show zero water blank in the first method. Adequate precision, accuracy and sensitivity were obtained in a direct method using 125I-aldosterone. Plasma aldosterone levels were 7.1+/-3.0ng/100ml (Mean +/-SD) in normal subjects and slightly higher after injecting ACTH-Z. The correlative coefficient between the first and the second method was significantly high (r=0.970, P less than 0.001, n=37). Plasma aldosterone was high (34.3+/-14.1ng/100ml, n=7) in primary aldosteronism, slightly high (14.2+/-2.6ng/100ml) in secondary aldosteronism, normal in Cushing's syndrome (10ng/100ml) and low in Addison's disease (1ng/100ml), hypopituitarism (1ng/100ml) and pseudoaldosteronism (2ng/100ml). From these results, it is concluded that the direct method without extraction was a very useful and reliable method for measuring plasma aldosterone. It was superior in simplicity and there is no need to use a liquid scintillation counter.

Addison Disease↗
PubMed1976

[Comparative study of diagnosis for location of adrenal adenoma in patients with primary aldosteronism (author's transl)].

Four methods; retroperitoneal air insufflation study, adrenal phlebography, adrenal scintiscan and estimation of plasma aldosterone in adrenal or renal veins, were compared to determine the diagnostic value for the location of adrenal adenoma in 27 patients with primary aldosteronism and one with idiopathic aldosteronism. The location of adrenal adenoma could be certainly determined, in 12 of 27 patients with primary aldosteronism by retroperitoneal air insufflation study, in 7 of 22 by adrenal phlebography, in 18 of 23 by estimation of aldosterone in adrenal or renal vein blood, and in 19 of 27 by adrenal scintiscan. From these results, it is concluded that firstly adrenal scintiscan with 131I-cholesterol and then adrenal phlebography and estimation of aldosterone in adrenal and renal vein blood combined with retroperitoneal air insufflation study should be performed for determination of location of adrenal adenoma in primary aldosteronism.

Adenoma↗
PubMed1976

Hypertension and plasma renin activity in experimentally induced chronic pyelonephritis.

The present authors conducted a series of experiments with mongrel dogs, in which unilateral chronic pyelonephritis was induced and postoperative changes in blood pressure and plasma renin activity were observed over a period of 3 months. The pyelonephritis infection was brought about by a method involving vesicoureteral reflux and ureteral dysfunction, as described in a previous paper. Pre- and postoperative levels of systolic pressure were compared, but no definite trends were found for the first 3 weeks after operation. At 30, 60 and 90 days the pressures were found to have risen by 21.2 +/- 14.6 and 17.1 +/- 16.7 mm Hg, respectively. No appreciable change in the plasma renin activity was found, however, at any stage. From these results it was concluded that experimentally induced chronic pyelonephritis in dogs caused a rise in blood pressure. No connection between the pyelonephritis and the renin angiotensin system was found.

Animals↗
PubMed1976

A case of juxtaglomerular cell tumor diagnosed preoperatively.

A case of juxtaglomerular cell tumor, the fourth case in the world diagnosed preoperatively, was reported. Neither strict sodium restriction nor loading of upright position increased a grade of hyperreninemia observed at recumbant position on ordinary diet, but Pald elevated in response to them. Abnormal glucose tolerance was considered to be due to hypopotassemia. Selective renal arteriography revealed a small number of tumor vessels, a lucent area on the nephrogram and early appearance of the renal vein of the diseased side. Histologically, the tumor contained some canalicular structures among the polygonal nonepithelial tumor cells with rhomboid bodies. These findings suggested that this tumor was a hamartoma originated from the juxtaglomerular apparatus.

Adolescent↗