Search PubMed⌕ Search

Biomedical subjects

S Fujii

Publications and source records attributed to S Fujii.

At least 739 records · Page 41Linked to original sources
PubMed1986

[Thymidylate synthase inhibition in malignant tumors after oral administration of UFT].

Surgical resection of solid tumors was undertaken five to six hours after the oral administration of UFT (300-500 mg/body) in five patients, who were (then) assayed for 5-fluorodeoxyuridylate (FdUMP), total dTMP synthase (TS) and non-FdUMP-bound, free enzyme. Three patients had gastric cancer, and two had mammary cancer. Control solid tumors were obtained without, the drug in eight patients. Six patients had gastric cancer. Eight had mammary cancer. Total TS averaged 2.04 p mol/g in the malignant tissues. TS inhibition averaged 40.1% to 57.1% in gastric cancer and 17.1% to 52.7% in mammary cancer. These data suggest that TS inhibition after oral administration of 5FU derivatives may be usefully studied for biochemical parameters of mechanisms of drug resistance.

Administration, Oral↗
PubMed1986

3,5,3'-Triiodothyronine regulates insulin level in the circulation following glucose ingestion in humans.

To clarify the relationship between the 3,5,3'-triiodothyronine (T3) response and the insulin response after glucose ingestion in humans, serum T3, immunoreactive insulin (IRI), C-peptide immunoreactivity (CPR), nonesterified fatty acid (NEFA) and plasma glucose levels were measured in 11 nonobese inpatients with normal glucose tolerance after oral 75 g glucose tolerance test. Ingestion of the glucose solution significantly increased serum T3 levels from 120.1 +/- 4.1 ng/dl to 132.5 +/- 4.4 ng/dl (P less than 0.01) at 60 min after glucose ingestion and it continued to increase after 3 h. Serum IRI and CPR levels peaked at 60 min (P less than 0.01, respectively) after glucose ingestion and they also continued to increase after 3 h. Plasma glucose levels peaked at 30 min (P less than 0.01) after glucose ingestion and then decreased to basal level after 3 h. Negative correlation was found between sigma T3 level and sigma IRI level (r = -0.75), and between sigma T3 level and sigma IRI/sigma glucose ratio (r = -0.88). Negative correlation was also found between basal T3 level and sigma IRI level (r = -0.82). No correlation was found between sigma T3 level and sigma CPR level. Positive correlation was found between sigma glucose level and sigma dT3 level (r = 0.74), and between sigma dglucose level and sigma dT3 level (r = 0.81). The findings suggest that T3 regulates insulin level in the circulation after glucose ingestion and the increase in serum T3 levels after glucose ingestion is necessary for the glucose removal from the circulation in humans.

Adult↗
PubMed1986

[An autopsy case of malignant lymphoma of the pancreas].

Case reports of malignant lymphoma of the pancreas are extremely rare. We experienced a 36-year-old man with malignant lymphoma involving the pancreas associated with obstructive jaundice, confirmed by autopsy. It is very difficult to differentiate between pancreatic carcinoma and pancreatic malignant lymphoma. Age, icterus, diffuse pancreatic enlargement, pathological findings and tumor markers may be useful in suggesting lymphoma. Trials of positive biopsy, appropriate radiotherapy and chemotherapy are necessary in patients who may have malignant lymphoma of the pancreas.

Adult↗
PubMed1986

The molecular structure of cyclonucleotide hexamer, CoGoCoGoCoGo having a high-anti conformation.

Cyclonucleotide hexamer, CoGoCoGoCoGo, was synthesized and crystallized as orthorhombic with space group C222(1), and unit cell dimensions: a = 48.30, b = 41.53, and c = 31.76A. The X-ray diffraction data up to 1.8A resolution were collected, and the crystal structure analysis by molecular replacement technique is now in progress using two energetically adequate left-handed helical models, which are obtained by conformational energy calculation.

Models, Molecular↗
PubMed1986

Development of interstitial cells and ovigerous cords in the human fetal ovary: an ultrastructural study.

Differentiation and subsequent regression of interstitial cells in the human fetal ovary was studied by light and electron microscopy in specimens obtained from twelve fetuses between 12 and 40 weeks of gestation. Interstitial cells possessing the ultrastructural features associated with steroid production first differentiate from fibroblast-like cells by 15 weeks. This occurs within the medullary fibrous tissue penetrating the cortex. The number of such cells is maximal at 18 weeks around the ovigerous cords in the inner half of the cortex. In addition, gap junction formation is seen in the pregranulosa cells forming ovigerous cords. From 21 to 31 weeks primordial follicles develop from ovigerous cords, which themselves develop from undifferentiated cortex, in a sequence that is most advanced in the medullary portion of the gonad and least advanced at its surface. During this time the interstitial cells decrease in number. They are not observed around the follicles, but are occasionally seen in the outer layer of the cortex which is still at the stage of ovigerous cords. At 40 weeks, growing follicles associated with several layers of enveloping theca cells are observed in the innermost region of the cortex. Interstitial cells are rare in all parts of the ovary. The development of interstitial cells independent of follicles in the human fetal ovary implies that such cells are distinct from theca cells, and that they correspond to the primary interstitial cells of other mammalian species. In addition anatomical and temporal relationships are observed between the interstitial cells, the ovigerous cords, developing primordial follicles and the formation of gap junctions in pregranulosa cells.

Cell Differentiation↗
PubMed1986

[Surgical approach for jugular foramen neurinoma--combined suboccipital and infralabyrinthine approach using a rotatable head holder].

A 36-year-old male with jugular foramen neurinoma was operated upon using a rotatable head holder, which enables the surgeon to rotate the patient's head at any time during the procedure and to gain access in multiple directions to the tumor. The tumor was situated primarily in the jugular foramen and showed partial extension into intracranial as well as into extracranial space. The patient was placed in the lateral position with a rotatable head holder, which allows rotation of the patient's head with the range of 10 degrees face up to 80 degrees face down from the horizontal plane. A linear skin incision was made, beginning behind the auricle and extending along the anterior margin of the sternocleidomastoid muscle, and the sternocleidomastoid muscle was divided just below the tip of the mastoid process. During mastoidectomy and suboccipital craniectomy, the patient's head was rotated 15 degrees face down and sigmoid sinus was exposed toward the jugular foramen, meanwhile the posterior fossa dura mater was opened and the intracranial portion of the tumor was removed with the head positioned 45 degrees-60 degrees face down. The patient's head is then turned 30 degrees face down and the facial canal was opened to displace the facial nerve forward. This oblique posterior approach minimized facial nerve displacement and provided excellent exposure of the large tumor rest which was situated mainly in the jugular foramen and partly extended extracranially. The rotatable head holder allows excellent access in multiple directions and is very helpful in approaching to jugular foramen neurinomas which grow primarily in the jugular foramen and extend both into intra- and extracranially.

Adult↗
PubMed1985

Modification of acyl-plasmin-streptokinase complex with polyethylene glycol. Reduction of sensitivity to neutralizing antibody.

Acyl-plasmin-streptokinase complex has advantages as a 'site' directed fibrinolytic agent with the active site protected from the plasma protease inhibitors. But, in clinical use, the fibrinolytic potential of this acyl-enzyme complex is modified or abolished by the presence of streptokinase antibodies in the patients. Therefore, better therapeutic agents are required. In this work, chemical modification of the acyl-plasmin-streptokinase complex with polyethylene glycol was found to result in marked resistance to neutralization with streptokinase antibodies.

Antibodies↗
PubMed1985

Complete amino acid sequence of bovine colostrum low-Mr cysteine proteinase inhibitor.

The complete amino acid sequence of bovine colostrum cysteine proteinase inhibitor was determined by sequencing native inhibitor and peptides obtained by cyanogen bromide degradation, Achromobacter lysylendopeptidase digestion and partial acid hydrolysis of reduced and S-carboxymethylated protein. Achromobacter peptidase digestion was successfully used to isolate two disulfide-containing peptides. The inhibitor consists of 112 amino acids with an Mr of 12787. Two disulfide bonds were established between Cys 66 and Cys 77 and between Cys 90 and Cys 110. A high degree of homology in the sequence was found between the colostrum inhibitor and human gamma-trace, human salivary acidic protein and chicken egg-white cystatin.

Amino Acid Sequence↗
PubMed1985

Analysis of serum lactic dehydrogenase levels and its isoenzymes in ovarian dysgerminoma.

The usefulness of serum lactic dehydrogenase (LDH) levels and its isoenzyme analysis as potential malignant ovarian tumor markers was investigated in 74 patients, including 5 cases of ovarian dysgerminomas. In this study 41.7% of patients with ovarian adenocarcinomas of the common epithelial types and 29.4% of cases with metastatic ovarian carcinoma had elevated preoperative serum LDH levels. In contrast all 5 cases of ovarian dysgerminomas had extremely high levels of serum LDH prior to therapy, whereas all 11 cases of benign solid ovarian tumors had normal LDH levels. The analysis of LDH isoenzymes revealed that ovarian dysgerminomas had high levels of the two fast fractions of LDH, whereas other malignant ovarian tumors had elevated levels of the two slow fractions. The present findings indicate that serum LDH levels and electrophoresis of its isoenzymes may be of value in patients with ovarian dysgerminomas.

Adult↗
PubMed1985

Sarcoma-like mural nodules combined with a microfocus of anaplastic carcinoma in mucinous ovarian tumor.

A case of mucinous ovarian tumor with sarcoma-like mural nodules, one of which coexisted with a microfocus of anaplastic carcinoma, was studied by light, electron microscopic, and histochemical examinations. The case suggested that the formation of sarcoma-like mural nodules may be the result of the proliferation of undifferentiated mesenchymal cells which exist beneath the mucinous epithelium by some stimulation like hemorrhage in the cyst wall. Histologically, the mononucleated cells composing the sarcoma-like mural nodules had epithelioid characteristics, particularly at the areas where the covering mucinous epithelium had already detached. The case also suggested that the findings of epithelioid characteristics in the sarcoma-like mural nodules themselves do not imply these nodules to be anaplastic carcinoma, but merely an epithelioid differentiation from undifferentiated mesenchymal cells, namely one of reactive products. However, the existence of a microfocus of anaplastic carcinoma combined with the sarcoma-like mural nodule necessitates a careful histologic analysis of mural nodules for the treatment of patients and the determination of the prognosis.

Adenocarcinoma, Mucinous↗
PubMed1985

Ultrastructural study of cultured smooth muscle cells from uterine leiomyoma and myometrium under the influence of sex steroids.

Upon the hypothesis that the differentiation or development of smooth muscle cells in both myometrium and leiomyomas may be under the regulation of sex steroids, myometrial and myoma cells were cultured in the presence of sex steroids and their morphological changes were studied ultrastructurally. The cells from the explants of both leiomyoma and myometrium at the 14th day in either control or E (10(-8) M 17-beta-estradiol)-added medium showed extremely well-developed cytoplasmic organelles with few filaments located at the marginal portion of the cytoplasm. These ultrastructural features resembled those of a dedifferentiated stage of smooth muscle cells in vitro. At the 21st day, these cells showed a moderate number of cytoplasmic filaments with dense bodies. These ultrastructural features resembled those of a redifferentiated stage of smooth muscle cells in vitro. In both E- and P (10(-7) M progesterone)-added medium, the cultured smooth muscle cells from both leiomyoma and myometrium showed an increased number of myofilaments with dense bodies even at the 14th day to the 21st day of culture in comparison to the cells either in control or estrogen-added medium. Therefore, the cultured smooth muscle cells in the medium with E and P lacked their dedifferentiated phase in vitro and/or the differentiation or maintenance of their filaments might be under the influence of both estrogen and progesterone. On the observation that the cultured smooth muscle cells from both leiomyoma and myometrium showed very similar ultrastructural changes under the influence of sex steroids, it was suggested that leiomyoma may have the same progenitor cells as myometrium.

Adult↗
PubMed1985

Optical recording of membrane potential responses from early embryonic chick ganglia using voltage-sensitive dyes.

Changes in absorbance of voltage-sensitive merocyanine-rhodanine dyes were used to monitor electrical responses in the semilunar ganglion of 4-10-day-old developing chick embryos. The electrical responses were simultaneously recorded from many positions in the ganglion. Stimulation of the afferent nerve fibers (the ophthalmic division of cranial nerve V) with a suction electrode led to changes in light absorption of the stained ganglia. With both the depolarizing and hyperpolarizing pulses, the change was largest at 700 nm and was eliminated at a wavelength of 620 nm where the voltage-dependent absorption change of the dyes disappears. In the 4-10-day-old embryonic ganglia, two types of optical membrane potential responses, 'non-conducted' and 'conducted' responses, were identified. The non-conducted response varied with the intensity of the stimulus and had the nature of an electrotonic spread. Furthermore, this non-conducted response exhibited an 'initial upstroke-response' followed by the steady-state plateau evoked by larger depolarizing pulses. The conducted responses were blocked by tetrodotoxin (TTX) or by high external potassium concentration. The incidence of the conducted responses increased as development proceeded from the 5th to the 10th day of age. Thus, the TTX-sensitive action potential activity is probably generated initially in the semilunar ganglion during the 5-10-day-stage of development. These data represent the first demonstration of membrane potential responses in early embryonic intact nervous system. Furthermore, these studies demonstrate the usefulness of voltage-sensitive dyes in the analysis of the organizing process of embryonic neuronal functions during these early stages of development.

Animals↗
PubMed1985

Induced fitting between a complex of four nucleotides and the cognate amino acid.

The conformation of the hydrogen-bonded complex of a trinucleoside diphosphate (anticodon bases), a nucleic acid base (discriminator base), and an amino acid is investigated. This complex has been named C4N (complex of the four nucleotides) by one of the authors. Concerning the aminoacylation of tRNA and the genetic code, it has been proposed that C4N accepts the cognate protein amino acid by the lock-and-key relationship. The purpose of the calculation is to investigate the conformational and energetic properties of C4N from the energy minimum principle. The calculation is carried out by using the empirical potential functions. Glycine, glutamine, and valine are taken as typical cases. The formation energies are estimated. It is shown that some conformational changes are induced in the anticodon trinucleoside diphosphate by the binding of the discriminator base. Conformational changes of C4N and the amino acid are also induced by the binding of the amino acid to C4N.

Amino Acids↗
PubMed1985

Distribution, ontogeny and projections of cholecystokinin-8, vasoactive intestinal polypeptide and gamma-aminobutyrate-containing neuron systems in the rat spinal cord: an immunohistochemical analysis.

The distribution, ontogeny and fiber projections of cholecystokinin-8, vasoactive intestinal polypeptide and gamma-aminobutyrate-containing neuronal systems in the rat spinal cord were investigated by means of immunocytochemistry. Immunoreactive fibers to cholecystokinin-8, vasoactive intestinal polypeptide and glutamate decarboxylase (gamma-aminobutyrate-synthesizing enzyme, used as a marker of gamma-aminobutyrate) were widely distributed in the spinal cord, being particularly concentrated in the superficial dorsal horn, suggesting a close relationship to the pain transmission system. Cholecystokinin-8-containing neurons were mostly distributed in the dorsal laminae and glutamate decarboxylase-containing neurons were distributed in both the dorsal and ventral horns. Vasoactive intestinal polypeptide-containing neurons were detected in the lateral spinal nucleus and the lamina X. Cholecystokinin-8 and vasoactive intestinal polypeptide immunoreactive structures first appeared on gestational day 17-18. Although no substantial change in immunoreactive structures was observed during the fetal period, they increased markedly after birth. On the other hand, glutamate decarboxylase-positive structures appeared at gestational day 16 and those in the grey matter reached a maximum content at birth; both groups were present in adult animals. Transection of the upper cervical cord resulted in accumulations of cholecystokinin-8 and glutamate decarboxylase rostral to the lesion, revealing the presence of supraspinal projections of cholecystokinin-8 and glutamate decarboxylase to the spinal cord. The same experimental procedure demonstrated the existence of vasoactive intestinal polypeptide-mediating neuronal projections to the supraspinal level, as the accumulating fibers occurred in the area caudal to the lesion.

Animals↗
PubMed1985

High glucose concentrations partially release hexokinase from inhibition by glucose 6-phosphate.

The phosphorylation of glucose by human erythrocyte hexokinase follows classical Michaelis-Menten kinetics; hexokinase manifests maximum activity at 5 mM glucose, and no further increase in activity can be measured at higher glucose concentrations. However, the erythrocytes of diabetics and normal erythrocytes incubated with high concentrations of glucose contain increased concentrations of glucose 6-phosphate. To elucidate the mechanism of accumulation of glucose 6-phosphate when erythrocytes are exposed to high glucose concentrations, hexokinase activity was examined in the presence of naturally occurring inhibitors, such as glucose 1,6-bisphosphate, 2,3-diphosphoglycerate, ADP, and glucose 6-phosphate at physiological concentrations. Without inhibitors or in the presence of glucose 1,6-bisphosphate,2,3-diphosphoglycerate, and ADP, maximum hexokinase activity was observed at 5 mM glucose concentration. On the contrary, in the presence of glucose 6-phosphate, hexokinase activity increased at glucose concentrations greater than 5 mM; inhibition by glucose 6-phosphate was partially competitive with glucose. The relief by glucose of glucose 6-phosphate inhibition of hexokinase is a possible explanation of the increased glucose 6-phosphate level in diabetic erythrocytes.

Diabetes Mellitus↗