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Biomedical subjects

S Friberg

Publications and source records attributed to S Friberg.

At least 91 records · Page 5Linked to original sources
PubMed1972

Two kinds of antigen suppression in tumor cells revealed by cell fusion.

The Ehrlich ascites tumor, which has been subjected to prolonged selection for growth in allogeneic hosts, possesses powerful mechanisms for the suppression of antigens normally found on the cell surface. It has previously been shown (1, 2) that when cells in which surface antigens are fully expressed are fused with Ehrlich cells, the suppressive mechanisms of the latter continue to operate and the new surface antigens introduced into the hybrid cell by the other partner are also suppressed. In the present paper we describe the properties of hybrids in which one parent cell was the TA3 ascites carcinoma. There are two sublines of this carcinoma which originally arose as a spontaneous mammary carcinoma in a strain A mouse. The TA3/St line has a high concentration of H-2(a) antigens and shows a strain-specific transplantation behavior; the TA3/Ha subline has a drastically reduced antigen concentration and readily transgresses histoincompatibility barriers. The immunoresistant TA3/Ha subline arose spontaneously without having been subjected to any known immunological selection pressure. Hybridization of TA3/Ha cells with normal diploid ACA fibroblasts reestablished full expression of H-2(a) antigens in nine independently derived hybrid clones. Full reestablishment of both D- and K-end components of the H-2(a) complex could be demonstrated. In some hybrid clones the concentration of H-2(a) antigens was found to be comparable to that seen in (A x ACA)F(1) fibroblasts, whereas in others a higher concentration was observed, even exceeding, in some cases, the levels found in the TA3/St line. The H-2(f) complex, contributed by the ACA parent cell, was fully expressed in eight of the nine hybrid clones studied. Antigen suppression thus behaves as a recessive character in the TA3/Ha hybrids, whereas in the Ehrlich hybrids antigen suppression is dominant.

Animals↗
PubMed1971

Expression of H-2 and Moloney leukemia virus-determined cell-surface antigens in synchronized cultures of a mouse cell line.

A mouse cell line derived from bone marrow (JLS-V9) was infected in vitro with Moloney leukemia virus. After the cell-surface antigens specified by this virus appeared, cells were synchronized in mitosis by a short treatment with colcemid. The expression of H-2- and virus-determined surface antigens was monitored during one cell cycle by an indirect membrane-immunofluorescence test. The highest proportion of antigen-positive cells was found during the G1 period; the proportion dropped as the cells entered the S period, and remained low until they entered the G1 period of the next cycle. The H-2- and virus-determined surface antigens were temporally coexpressed.

Animals↗