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Biomedical subjects

S Eriksson

Publications and source records attributed to S Eriksson.

At least 505 records · Page 28Linked to original sources
PubMed1977

Presymptomatic primary biliary cirrhosis.

Clinical, laboratory and follow-up results in 13 patients with primary biliary cirrhosis (PBC) collected during the last 10 years in a well defined population of 250,000 inhabitants are presented. The mean observation time was 5.5 years. 77 percent of these patients have been asymptomatic for many years. Characteristic laboratory features in asymptomatic patients are high alkaline phosphatases and glutamyl transpeptidases and very high levels of polyclonal IgM. ESR is often increased. Signs of active cell destruction are slight and functioning cell mass is well preserved. High titers of mitochondrial antibodies are consistently present. Needle biopsy is seldom sufficient for diagnosis but permits staging of the disease. There is no correlation between clinical features and histological evolution stage. Compared with a preceding 10-year period, the incidence of PBC has risen threefold. This increase can be fully explained by the extended use of laboratory facilities, resulting in the detection of asymptomatic patients.

Adult↗
PubMed1977

A chromatographic procedure for the purification of human plasma albumin.

Albumin is obtainable from human blood plasma by an ion exchange chromatographic procedure in a yield of about 95% and a purity well above Pharmacopoeia requirements. Cryosupernatant, factor IX depleted plasma is precipitated with 12 and 25% w/v polyethylene glycol 4000. The second precipitate is dissolved to 8% w/v protein and applied to a DEAE-Sephadex A-50 or a DEAE-Sepharose CL-6B column. Albumin is further purified by chromatography on SP-Sephadex C-50. Gel filtration on Sephadex G-25 is used for desalting prior to lyophilization. The process has been initially designed for fractionation of 50 litres plasma/week but can be further scaled up to meet considerably higher capacity requirements.

Chromatography, Ion Exchange↗
PubMed1977

Liver function in asymptomatic adult individuals with severe alpha1-antitrypsin deficiency (Pi Z).

Eleven adult individuals (aged 24 to 66 years) with severe alpha1-antitrypsin deficiency, Pi Z, and with no clinical signs of liver disease, were investigated with a broad spectrum of liver function tests. Except for low alpha1-antitrypsin levels, no evidence of abnormal hepatic protein synthesis was found. Neither could any biochemical signs of liver cell necrosis or increased liver cell regeneration be disclosed. In spite of the well-known pronounced distension of hepatic endoplasmic reticulum (E.R.) by aggregated asialo-alpha1-antitrypsin in this disease, the function of the E.R. tested with the aminopyrine breath test was not found to be impaired.

Adult↗
PubMed1977

Lipid islands in the gastric mucosa after resection for benign ulcer disease.

Lipid islands in the gastric mucosa are macroscopically well demarcated white or yellow-white patches, microscopically composed of accumulations of lipid-filled macrophages. The occurrence of lipid islands has not previously been linked to gastric surgery. This study presents the prevalence of lipid islands after Billroth I and Billroth II resection as well as in a nonoperated control group. Gastroscopy and multiple biopsies were performed at selected intervals after surgery. The occurrence of single or multiple lipid islands increased with the postoperative time, independent of the original ulcer disease or the type of operation performed. In nonoperated patients, lipid islands were found in 6.3%, and approximately the same prevalence was found 1 to 3 years after gastric surgery. A much higher prevalence was found 10 to 15 years postoperatively, and 23 years after Billroth II resection 60% of the patients demonstrated lipid islands. At repeated gastroscopic examinations over a period of 1 to 2 years, no certain changes in number, size, or distribution of the lipid islands were seen. The etiology of lipid islands is unknown, but intestinal-gastric reflux may be the most plausible etiological factor.

Adult↗
PubMed1977

In vitro responsiveness of human gastric carcinoma to pentagastrin.

The influence of pentagastrin on thymidine incorporation of five human gastric carcinomas was studied by an in vitro short-term cell suspension technique. Two of the five tumours reacted with stimulation of incorporation to the treatment. Five tumours of other aetiology did not react to similar treatment. The possibility of gastric carcinoma as a hormone-sensitive tumour is discussed.

Breast Neoplasms↗
PubMed1976

The serum sialyltransferase activity in alpha 1-antitrypsin deficiency.

alpha 1-Antitrypsin phenotypes Pi M and Z, purified by the thiol-disulfide exchange procedure, were desialylated by treatment with neuraminidase covalently coupled to Sepharose and used as acceptors of sialic acid in an assay system for serum sialic acid transferase (CMP-N-acetylneuraminate:D-galactosyl-glycoprotein N-acetylneuraminyltransferase, EC 2.4.99.1) activity. Both asialoantitrypsins were equally effective as acceptors in contrast to native Pi Z antitrypsin which did not accept any sialic acid. Serum sialyltransferase activity was determined in 38 adult alpha 1-antitrypsin deficient individuals (Pi Z, MZ, FZ, SZ) with normal liver function and was found to be of the same magnitude as the activity in normal individuals (Pi M). Equal activities were also found in 5 Pi Z patients with cirrhosis of the liver. The results strongly argue against the concept that sialyltransferase deficiency provides the molecular basis for alpha 1-antitrypsin deficiency.

Fasting↗
PubMed1976

Late precancerous changes and carcinoma of the gastric stump after Billroth I resection.

Carcinoma of the gastric remnant after resection for benign ulcers is regarded as a rare complication after Billroth I resection. In seventy-four patients reexamined by gastroscopy and multiple biopsies ten to twenty-two years after gastric resection four cases of cancer of the gastric stump were diagnosed and the patients operated on. Another eight patients had regenerative polyps and one patient had a tubular adenoma. The histologic findings in gastroscopic biopsy specimens from the gastric stoma and fundus are compared. Possible precancerous changes and etiologic aspects are discussed briefly. Repeated gastroscopy is recommended starting about ten years after gastric resection for benign ulcers.

Adenocarcinoma↗
PubMed1976

Lung function studies in asymptomatic individuals with moderately (Pi SZ) and severely (Pi Z) reduced levels of alpha1-antitrypsin.

Extensive physiological evaluation of lung function were conducted in two groups of asymptomatic individuals with deficiencies of alpha1-antitrypsin: seven subjects with phenotype Pi SZ and six subjects with phenotype Pi Z. Four symptomatic individuals of Pi Z type were also investigated. The asymptomatic individuals of both SZ and Z phenotypes revealed reduction of elastic recoil, hypoventilation and hypoperfusion of the lung bases with increased closing and nitogen wash-out volumes. No signs of bronchial obstruction were observed. The asymptomatic Pi SZ subjects revealed as many signs of subclinical disease as did the asymptomatic Pi Z subjects. These data suggest that Pi SZ subjects are at increased risk of emphysema similar to Pi Z subjects.

Adult↗
PubMed1976

Serum isoamylases in chronic pancreatitis.

Serum isoamylases were quantitated separately in 15 patients with chronic pancreatitis. The mean activity of pancreatic isoamylase was 7 U/1 in the patients as compared to 88 U/1 in healtly controls, 8 of the patients completely lacked pancreatic isoamylase and the mean activity in the other was 15 U/1. Salivary isoamylase activities were moderately increased in the patients. Determination of the serum pancreatic isoamylase is a simple screening procedure that should be useful in the diagnosis of exocrine pancreatic insufficiency.

Adult↗
PubMed1975

Characterization of alpha1-antitrypsin in the inclusion bodies from the liver in alpha 1-antitrypsin deficiency.

alpha1-antitrypsin was isolated from periodic acid-Schiff-positive inclusion bodies from the hepatocytes of patients with alpha1-antitrypsin deficiency and further purified to enable more detailed chemical analysis. Amino acid and cyanogen bromide fragmentation studies showed a close similarity between hepatic and serum (PiMM) antitrypsin in contrast to the carbohydrate analysis, which revealed markedly deficient glycosylation of hepatic antitrypsin. A complete lack of sialic acid and a relative deficiency of all other carbohydrate components could fully explain the difference of approximately 6000 daltons in molecular size between the two proteins. The accumulation of hepatic globules is probably related to the physical properties of the defective antitrypsin, which include marked insolubility and tendency toward aggregation. The results strongly suggest an abnormal amino acid sequence in the peptide chain of the deficient antitrypsin. The interference with glycosylation may be related to steric hindrance.

Amino Acid Sequence↗
PubMed1975

Ribonucleotide reductase from Escherichia coli: demonstration of a highly active form of the enzyme.

Ribonucleotide reductase from Escherichia coli consists of two nonidentical subunits, proteins B1 and B2. The activity of the enzyme in crude extracts prepared from mechanically disrupted bacteria is very low. Enzyme activity is stimulated 5 to 10-fold by addition of an excess of either subunit. Concentrated extracts from cells lysed gently on Cellophane discs (Schaller et al.) contained 10 to 20-fold higher activity than extracts from mechanically disrupted cells. This activity was not further stimulated by either B1 or B2. The system is suitable for complementation tests for the analysis of temperature-sensitive mutants affecting the ribonucleotide reductase system. Concentrated high-speed supernatants from E. coli treated with lysozyme (Wickner et al.) also contained a high ribonucleotide reductase activity, which was stimulated slightly or not at all by addition of B1 and B2. This active form of the enzyme was unstable and could not be purified. The results suggest that the intracellular form of the enzyme consists of a tight complex of proteins B1 and B2, possibly stabilized by other intracellular structures.

Adenosine Triphosphate↗