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Biomedical subjects

S Cox

Publications and source records attributed to S Cox.

187 records · Page 11Linked to original sources
PubMedDate not supplied

Effects of flutamide and hydroxy-flutamide on the growth of human benign prostatic hyperplasia cells in primary culture: a preliminary report.

Tissues from human benign prostatic hyperplasia [BPH] were collected from twelve patients undergoing routine transurethral resection of the prostate to relieve urine out-flow obstruction. Viable epithelial organoids were obtained after enzymatic digestion of the tissue. Primary cultures of epithelium were successfully maintained on collagen gel for up to 21 days. Immunocytochemical staining revealed that there was no expression of either desmin or vimentin in these cells; however, the anticytokeratin antibodies LP-34 (cytokeratins 4, 5, 6, 10, 13, 16, 17 and 18), LE-61 (cytokeratin 18) and CAM 5.2 (cytokeratins 7 and 8) all showed positive responses, indicating the epithelial nature of the cells. Cell growth was significantly increased in the presence of 3 x 10(-10) M testosterone propionate [TP] in the culture medium. The presence of the non-steroidal anti-androgens, Flutamide and Hydroxy-Flutamide [Flu-OH], in the concentration range 1.0-0.001 micrograms per ml of medium inhibited the growth in the presence of androgens in a dose-dependent manner. The anti-androgens failed to affect cell growth in the absence of TP. In view of these preliminary findings, it is postulated that the antiandrogens might be acting either by displacing the androgen from its receptor or alternately by inhibiting the activity of prostatic 5 alpha-reductase.

Aged↗
PubMedDate not supplied

Evaluation of APHA and AOAC methods for phosphatase in cheese.

Varieties of market cheese were analyzed for alkaline phosphatase by the modified rapid colorimetric method of the American Public Health Association (APHA) and the official AOAC method, 16.304-16.306. In the APHA method, 5 g cheese (pH less than 7.0) is macerated with 2 mL 1:1 carbonate buffer, or 2 mL water (for cheese with pH greater than 7.0). Addition of 0.1 mL magnesium acetate (1 mg magnesium) to test portions of cheese extracts yielded reproducible and quantitative recovery of added phosphatase. In the AOAC method, macerating 0.5 g cheese with 1 mL borate buffer before adding milk phosphatase improved recovery among cheeses. Addition of magnesium ion increased phosphatase activity in some cheeses. Phosphatases in blue mold-ripened and Swiss cheeses were inactivated by heat faster than was milk phosphatase, yet milk phosphatase added to various soft cheeses was completely inactivated at 60 degrees C for 10 min. The lability of phosphatase was due to the heat-denaturing effect of NaCl present in finished cheeses. Some Mexican style soft cheeses contained both heat-labile and heat-stable phosphatases. These data suggest that the phosphatase test to differentiate milk and microbial phosphatases on the basis of repasteurization and analysis of cheese is no longer valid.

Animals↗
PubMedDate not supplied

How I coped emotionally with diabetes in my family.

Many people need to go through a period of emotional adjustment before they can accept their diabetes. Much skill, patience and understanding is needed from the health professional to help them through this process. Many diabetic people go through a pattern of emotional reactions which are similar to grief reactions. These include shock and disbelief, denial, anger, depression and withdrawal when they realise the diabetes will not go away, and mourning for the loss of a life-style that was comfortable, familiar, secure. They also feel the loss of indulging in things they once found pleasurable, and the loss of independence. Finally comes the stage of acceptance. The person's fears of developing long-term complications-going blind, having heart disease, developing gangrene and so on-need to be addressed fairly early on. Education about avoiding complications is important but may not be effective until the person has worked through feelings of fear and grief. To the health professional it may seem astonishing that the patient does not comply with the treatment and accept the good advice that is given, but these reactions can often be understood in the light of the emotional adjustments that are taking place. Knowledge alone is not enough. Although health professionals need to give patients information and advice, they also need to help them express their feelings and provide emotional support during the adjustment process.

Adaptation, Psychological↗