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Biomedical subjects

S Barros

Publications and source records attributed to S Barros.

At least 19 recordsLinked to original sources

Aseptic peritonitis in a peritoneal dialysis patient.

INTRODUCTION: Patients who have repeatedly sterile peritoneal fluid cultures despite elevated peritoneal fluid white cell count should be evaluated for disorders other than usual bacterial peritonitis. Intra-abdominal pathology was responsible for less than 6 percent of cases of peritonitis. Still, the clinical outcome is these situations are much worse than in other commoner causes. CASE REPORT: A 25-year-old male non-diabetic patient in PD started his complains with diffuse abdominal pain with spontaneous remissions and exacerbations, anorexia and vomiting with 3 days evolution. Laboratory results with persistent culture-negative peritoneal fluid results seemed compatible with the diagnosis of aseptic peritonitis. However, clinical status progression and peritoneal fluid amylase levels above 50 UI/L led to perform an abdominal ultrasound that showed a painful non-compressible tubular structure with a diameter of >6 mm at the base of the cecum. The patient was then submitted to a laparotomy with appendix removal. DISCUSSION: When assessing a patient with abdominal pain and clear or cloudy but aseptic peritoneal liquid, causes other than peritonitis should be excluded. Under antibiotic therapy, their clinical picture and evolution may be masked, delaying surgical resolution. In appendicitis, this delay may lead to perforation and consequent faecal peritonitis. All patients should be screened for peritoneal fluid amylase levels in order to differentiate bacterial peritonitis from intra-abdominal pathology. In all cases similar to the present one, an abdominal US/CAT scan should be promptly made.

Adult↗

Influence of transposed stomach on cardiac function in patients with resected esophageal cancer.

Although the use of the posterior mediastinum and the stomach as a reconstruction option after esophagectomy has wide acceptance, there are concerns about the potential cardiac impairment it could cause. We prospectively studied 27 patients regarding the function and the systolic diameter, diastolic diameter, shortening fraction, ejection fraction and the presence of extrinsic compression. The patients were studied preoperatively and between the 45th and 60th postoperative days. The parameters were still within normal clinical ranges. We concluded that this type of reconstruction does not harm the patients in terms of their cardiac function.

Adenocarcinoma↗

Regulation of cell morphology and cytochrome P450 expression in human hepatocytes by extracellular matrix and cell-cell interactions.

The influence of extracellular matrix conditions and plating density on cell cytoarchitecture and the constitutive and chemically induced expression of cytochrome P450 3A4 (CYP3A4) was examined in primary cultures of human hepatocytes. Constitutive and drug-induced microsomal CYP3A4 expression occurred equally well in human hepatocyte cultures maintained on either a complex or simple substratum (Matrigel vs collagen, type I), or in a sandwich configuration (i.e., between two layers of extracellular matrix), despite the markedly different morphological properties exhibited by each condition. However, a density-dependent decrease in both the constitutive and induced levels of CYP3A4 was observed in hepatocytes maintained on a simple collagen substratum as plating density was reduced from 100% to 25%. Marked alterations in cell shape and cytoarchitecture were noted concomitant with decreases in the expression and localization of intercellular gap junctions and E-cadherin-mediated cell adhesions. In addition, the intracellular distribution of microtubules and microfilaments was altered substantially and the expression of immunoreactive actin and beta-tubulin increased as cell density was decreased. These effects were reversed to some extent by overlaying monolayers with extracellular matrix or by co-culturing with another cell type. Efforts to maintain normal cell shape and cytoskeletal distribution in hepatocytes at low cell density with a Matrigel substratum failed to restore normal basal levels of CYP3A4 expression or responsiveness to rifampicin (RIF). Likewise, E-cadherin and Cx-32 expression was again reduced, even though the distribution and expression of cytoskeletal elements returned to normal levels. These results suggest that cell-cell contacts, but not the extracellular matrix configuration or composition, play a critical role in determining normal responsiveness to chemical modulators in human hepatocytes.

Actins↗

[Experience of a nursing student in an interdisciplinary team: factory of ideas].

The experience of the Curricular Training in a mental health work attendance to out-patients, CAPS, lead to this study development in the attempt to understand and characterize interdisciplinary team in this institution, as well as to understand the admittance of a nursing student in this team. The analysis of the replies disclosed that in the reports is found the concept of interdiscipline as well as of the multidiscipline (work in compartments). The conception which has of the model of assistance and of the admittance of the project in it is compatible with the conceptions that establish the description of the work: flexibility, the projects inter-relation the enlarged practice and the psychosocial rehabilitation. The fact that the service has a Program of lecturing--Assistance Integration, "naturalizes" and validates the participation of a nursing student in the projects of assistance or sociability.

Humans↗

Effect of troglitazone on cytochrome P450 enzymes in primary cultures of human and rat hepatocytes.

1. Troglitazone was the first thiazolidinedione approved for clinical use in the treatment of non-insulin-dependent diabetes mellitus. During clinical investigations of drug-drug interactions with therapeutics (terfenadine and cyclosporine) known to be metabolized by CYP3A4, pharmacokinetic interactions were noted upon troglitazone multiple-dose treatments. The nature of the interactions suggested induction of CYP3A enzymes. 2. Primary cultures of human hepatocytes were used to investigate the induction potential of troglitazone with respect to CYP3A4, CYP2B6 and CYP1A1/2. In human hepatocytes, troglitazone induced both immunoreactive CYP3A4 protein and testosterone 6beta-hydroxylase activity in a dose-dependent fashion (EC50 = 5-10 microM), accompanied by an increase in CYP3A4 mRNA. The capacity of troglitazone to induce CYP3A4 was between that of rifampin (EC50 = 0.8 microM) and dexamethasone (40-50 microM). Troglitazone increased CYP2B6 immunoreactive protein but did not significantly effect CYP1A1/2 activity, immunoreactive protein or mRNA. 3. Troglitazone produced significant increases in CYP3A message, protein and activity in primary rat hepatocytes, a slight increase in CYP2B1/2 activity and no change in CYP1A1/2 message or activity. 4. These results provide evidence that troglitazone can induce CYP3A and CYP2B enzymes while apparently not altering CYP1A. This provides a rationale for the clinically observed interactions of troglitazone with selected CYP3A4 substrates.

Animals↗

[The process of nursing care in mental health].

This is an essay about the nursing working process in mental health. Several conceptions have substantiated the psychiatric assistance, as well as the proposals of reorientation of those practices; nursing practices have been related to that process. The professionals' standpoint determines the choice of a tendency and the understanding of the health-illness process. The need of reviewing the nursing working object, as well as its practice in view of the transformations in mental health assistance, has pointed towards new professional attitudes regarding the person with psychic disorders, as well as towards the acquisition of knowledge besides that one obtained at college courses.

Humans↗

Development of a monoclonal antibody blocking-ELISA for detection of antibodies against Maedi-Visna virus.

A monoclonal antibody (MAb) blocking ELISA (Blck-ELISA) was developed to detect antibodies against Maedi-Visna virus (MVV) in sheep sera. The assay employs a MAb directed against the envelope protein p90 of the virus in a sandwich blocking procedure. To determine whether the MAb was a potential antibody for developing a Blck-ELISA, a collection of three hundred sera obtained from several sheep flocks known to be infected with MVV were used to examine the sensitivity of the Blck-ELISA. A total of 50 serum samples originating from a flock free of MVV were tested to assess the specificity of the assay. The results were compared with a commercial indirect ELISA (I-ELISA) and samples giving a conflicting or doubtful result were tested by immunoblot. The Blck-ELISA proved to be specific, sensitive and it showed high reproducibility and low variability.

Animals↗

[Attempts at innovations in the practice of teaching and practicing psychiatric nursing].

In the context of recent changes that are taking place in the field of mental health care in Brazil, the University has the responsibility to redimensioning human resources qualification of mental health workers. At the Mental Health Care and Educational Program of the University of São Paulo and São Paulo Health Office (São Paulo/Brazil), an experience still in course, the nursing students attending to psychiatric nurse training evaluated that theoretical-practical education dealing with health care issue in the field of Psychosocial Rehabilitation purpose, at the same time that directly interferes in the qualification of practices, simultaneously develops the students attitudes and knowledge that qualify them to perform coherent practices in accordance with rehabilitation models of care.

Attitude of Health Personnel↗

[Graduation from nursing: learning about the Brazilian psychiatric reform].

This paper aims at identifying the changes that became part of the teaching of psychiatric nursing. The study points out the concern regarding teaching updating in this area in which the teacher's transformation, participation and the creation of a new concept about psychiatric knowledge and practice make it possible to have new ways to learn how to learn the brazilian psychiatric reform.

Brazil↗

The influence of the transposed stomach through the posterior mediastinum on the respiratory forced expiratory volume and forced vital capacity in patients with resected esophageal cancer.

Although the use of the posterior mediastinum and the stomach as a reconstruction option after esophagectomy has large acceptance all over the world, there are concerns about the potential respiratory impairment it could cause. We prospectively studied 35 patients regarding the forced expiratory volume and vital capacity. The patients were studied preoperatively and between the 45th and 60th postoperative days. The value of both parameters decreased, although they were still within normal clinical ranges. We concluded that this type of reconstruction does not harm the patients regarding the respiratory flow rates.

Adenocarcinoma↗

4-Nerolidylcatechol from Pothomorphe spp. scavenges peroxyl radicals and inhibits Fe(II)-dependent DNA damage.

The total reactive antioxidant potential (TRAP) and total antioxidant reactivity (TAR) of 4-nerolidylcatechol (4-NC) and methanolic extracts of Pothomorphe umbellata and P. peltata were determined by monitoring the intensity of luminol enhanced chemiluminescence by peroxyl radicals derived from thermolysis of 2,2'-azobis(2-amidinopropane). The highest antioxidant potential was measured in the extract of P. umbellata (TRAP = 97.2 microM) while the highest reactivity was observed in the extract of P. peltata (TAR = 5.0 microM), measured as equivalents of Trolox concentration. These results were higher than those obtained for 4-NC (TRAP = 33.6 microM, TAR = 4.9 microM). DNA sugar damage induced by Fe(II) salts was also used to determine the capacity of 4-NC to suppress hydroxyl radical-mediated degradation of DNA. Calculated IC50 values for 4-NC and catechin, used as a standard, were 25 and 17 microM, respectively.

Catechols↗

Development of anti-human B blood group monoclonal antibodies suitable as a blood typing reagent.

An improved procedure for the generation of high-avidity anti-human B blood group monoclonal antibodies (MAbs) was developed. One of them, termed 7A1-2, showed excellent qualities of titer, avidity, and intensity required for use as human B blood typing reagent. Hemagglutination inhibition studies with monosaccharides and oligosaccharides were carried out to determine the specificity of the MAb 7A1-2. These studies indicate that the antibody reacts with the immunodominant region of the antigen which is known to confer the serologic specificity of this blood group.

ABO Blood-Group System↗

[Nursing care in psychiatric emergencies].

The emergency psychiatric services implantation in general hospitals, at São Paulo City, motivated the authors to write this article. They present some considerations about psychiatric emergencies and the main points in nursing care of the client in a crisis intervention setting.

Aggression↗

[The nurse in mental health outpatient clinics].

This study emerged from the preoccupation to evaluate the learning needs of psychiatric nurses working in an ambulatory of Mental Health. We verified the type of education they had, the actions they performed and the difficulties and facilities they found in the development of nursing interventions. It was also examined whether these actions are in conformity to the ones prerecognized by the literature or not. The data analysis showed that the majority of the actions are performed by nurses in distinct manners in 6 ambulatories. We perceived, in some events, the "function deviation" in nursing activities of nurse and the nursing team.

Ambulatory Care Facilities↗

[The nursing care of patients with a behavioral manifestation arising from a withdrawal from reality].

With this study the authors want to furnish the nurses with one more reference source to guide their actions in caring for the patient with manifestation of reality withdrawal. Some concepts of withdrawal are presented and, summarily, the clinical manifestation. Nursing care is described considering their problems and general measures to be taken into account for patients individuality.

Behavior↗