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Biomedical subjects

S Asano

Publications and source records attributed to S Asano.

At least 523 records · Page 29Linked to original sources
PubMed1980

Cytological characters, transformation, and ontogenesis of dermal histiocytes and fibroblasts of rats.

Cell morphology of histiocytes and fibroblasts was observed light- and electron microscopically in the subcutaneous connective tissue of unstimulated adult rats. Striking ultrastructural differences between them were noted in the cell surface characters, extent and development of endoplasmic reticula, especially of rough-surfaced ones, and volumes of lysosomal components. Rosetting assays revealed that histiocytes were positive for EA- and EAC-rosette formation and capable of engaging in immunophagocytosis. However, fibroblasts were negative for both rosettings. In the experiments of vital staining with lithium carmine, Fesin phagocytosis, carrageenin granuloma formation, in vivo culture by intraperitoneal implantation of diffusion chambers, and subculture, cytological changes of histiocytes or macrophages and fibroblasts, as well as their transformation, were investigated. Although fibroblasts often simulated histiocytes under certain stimulated conditions, no transformation of fibroblasts into histiocytes was confirmed. Ontogenetically, histiocytes already existed in the subepidermal mesenchyme of rat fetuses before differentiation of undifferentiated mesenchymal cells into fibroblasts and showed no relationship to the fibroblasts. Ultrastructural cell morphology and cultural characteristics of the fetal histiocytes were presented and their possible origin was discussed briefly.

Animals↗
PubMed1980

Sinus histiocytosis with massive lymphadenopathy in a Japanese adult with unusually elevated EBV antibody titers.

A 35-year-old Japanese male was presented with massive lymphadenopathy in bilateral preauricular and submandibular regions for five years' duration without any complaints despite of no specific treatments. Laboratory examinations revealed neurtrophilia, elevated BSR, hypergammaglobulinemia with elevated IgG and IgM, positive CRP and RA, low percentage of T-cells in peripheral blood, impaired PHA blast transformation, and elevated EBV titers against viral capsid, early and nuclear antigens. Biopsy specimens demonstrated massive histiocytosis with hemophagocytosis in the sinuses and predominant mature plasma cells in the medulla, which were fairly well consistent with "sinus histiocytosis with massive lymphadenopathy" (ROSAI and DORFMAN). Ultrastructural study revealed histiocytes exhibiting avid phagocytosis of blood cells, epithelioid histiocytes with poor phagocytic activity, foamy storage histiocytes loaded with a large number of osmiophilic lipid granules and giant cells of various types. Pathogenesis of this apparently benign disease entity was briefly discussed, and its refractoriness against any specific therapy was emphasized.

Adult↗
PubMed1980

Localized blastoma preceding blastic transformation in Ph1-positive chronic myelogenous leukaemia: morphological and cultural studies of the transformation event.

In a patient with Ph1-positive chronic myelogenous leukaemia (CML), the development of a localized blastoma preceding generalized blastic transformation was accompanied by a reduction of granulocyte-macrophage (GM) colony forming capacity in agar of bone marrow cells as well as an increase in peripheral neutrophil alkaline phosphatase (NAP) scores. Successful transplantation of the blastoma cells into nude mice enabled extensive studies of the cell properties. The blastoma cells were double Ph1-positive cells which did not respond to human granulocyte-macrophage colony-stimulating factor (GM-CSF), and were similar to cells demonstrated in spleen and bone marrow at the terminal stage of the patient's illness. These observations clearly support the case for sequential studies of colony formation in vitro as a useful test for the early detection of disturbances in marrow function that may occur before generalized blastic transformation. Studies of the properties of the blastoma cells also provide some insight into possible mechanisms for the transformation event.

Aged↗
PubMed1979

Granulocytosis and colony-stimulating activity (CSA) produced by a human squamous cell carcinoma.

A patient with a squamous cell carcinoma accompanied by a marked granulocytosis (100,000/mm3) of unknown origin was examined for Colony-Stimulating Activity (CSA). The pleural fluid and the tumor extract revealed high CSA. The floating cells in the pleural fluid were successfully transplanted into nude mice as a localized tumor with cyst formation. The tumor invariably caused a marked granulocytosis (100,000--300,000/mm3) with induction of a conspicuous splenic granulopoiesis in the transplanted mice. High CSA were demonstrated in their cystic fluid as well. Media conditioned by the primary cultures of these tumor cells revealed the same CSA, demonstrating the direct production of CSA by the tumor itself. These results indicate the presence of human CSA producing tumor and that such a tumor may in part account for a marked granulocytosis of unknown origin observed in some patients with cancer.

Adult↗
PubMed1979

Changes in intracellular activities of lysosomal enzymes in tissues of rats during aging.

A comparison of results obtained from studies of the intracellular fractions of the tissues of liver, brain and heart of "young" (1-2 months), "old" (24-27 months) and "senile" (34-37 months) rats showed that the ratios of three enzymes, acid phosphatase, beta-N-acetylglucosaminidase and acid RNase of the liver and heart were very similar and their activities decreased with age. On the other hand, the protein content is the supernatant of the liver, and acid DNase activities in the supernatant of the brain increased significantly with age. When the 24-27 month and 34-37 month old rats were compared, the ratios of the total activities of liver beta-N-glucosaminidase and brain acid DNase in the supernatant and the specific activities of brain beta-N-glucosaminidase in the microsomal fraction increased significantly.

Acid Phosphatase↗
PubMed1979

Transitional cell carcinoma arising from bladder left behind.

A case of transitional cell carcinoma arising from a bladder which was left behind for more than 24 years without pyocystis syndrome is described. A search in the literature revealed 10 cases of defunctionalized bladder that developed tumors over a rather short latent period ranging from 3 months to 7 years, and mostly with evidence of severe bladder infection.

Carcinoma, Transitional Cell↗
PubMed1979

Effects of methylmercuric chloride intoxication on the intracellular activity of lysosomal enzymes in rat liver and brain.

The activity of the lysosomal enzymes of rat liver after a single intraperitoneal injection of methylmercuric chloride (5.0 mg/kg) was enhanced in the nuclear fraction during early post-injection (2 hr to 7 days), while it decreased in the mitochondrial fraction and increased in the lysosomal fraction during late post-injection (14 days). Rat brain activity, however, was reduced in the nuclear, mitochondrial and lysosomal fractions during late post-injection (14 days). A high accumulation of total mercury was observed in the liver during early post-injection (36 hr and 7 days) while similar accumulation in the brain occured during late post-injection (7 days and 14 days). Both the mercury burdens and the enzyme activities of the rat liver and brain returned to normal levels within 30 days.

Animals↗
PubMed1979

Analysis of the process of cell degradation induced by Triton X-100 in Ehrlich ascites tumor cells.

Analysis of the liberation degrees of intracellular materials from Ehrlich ascites tumor cells by treatment of Triton X-100 was performed. The liberation process has two stages. In the first stage, cytoplasmic inorganic phosphorus were liberated at a concentration of about 0.01% of the detergent, and in the second stage macromolecules such as lysosomal enzymes were liberated at a concentration of about 0.03% of the detergent. Morphologically, the cells began to swell in the first stage but were not stained by Trypan blue, and the cells in the second stage became fully swollen and ruptured. The staining curve of the swollen and ruptured cells was closely in parallel with the liberation curves of the macromolecules.

Animals↗
PubMed1978

An ultrastructural study of localized lymphangioma circumscriptum.

An electron microscopic study was performed on the lymphatic vessel (lv) of localized lymphangioma circumscriptum (Lc), which was found clinically typical but histologically not associated with hemangioma and inflammation. The lv of Lc was morphologically divided into two types. The first type was situated at the upper dermis of the lesion and the other, at the deep dermis. The former had certain similarities to lv of experimentally induced lymph-stasis, whereas the latter was morphologically similar to lv of the normal skin. From the findings of the present study and those reported in literature, Lc could not be considered as a mere benign overgrowth of lv, but as one of pathological results induced by a long-standing lymph-stasis.

Adult↗
PubMed1978

A case of red-cell adenosine deaminase overproduction associated with hereditary hemolytic anemia found in Japan.

A case of red cell adenosine deaminase (ADA) overproduction associated with hereditary hemolytic anemia is reported here. This appears to be the second report. Proband is a 38-year-old Japanese male who had hemoglobin, 15.8 g/100 ml; reticulocyte count, 4.5%; serum indirect bilirubin, 4.9 mg/100 ml; 51Cr-labeled red cell half-life, 12 days; red cells showed moderate stomatocytosis. His red cell ADA activity showed 40-fold increase while that of the mother showed 4-fold increase. The mother was hematologically normal. The father had a normal enzyme activity. The proband and the mother showed slightly high serum uric acid levels. The proband's red cell showed: ATP, 628 nmoles/ml (normal, 1,010--1,550); adenine nucleotide pool, 46% of the normal mean; 2,3-diphosphoglycerate content, 3,782 nmoles/ml (normal 4,170--5,300); increased oxygen affinity of hemoglobin, P50 of intact erythrocytes being 21.8 mmHg (normal, 24.1--26.1). Red cell glycolytic intermediates in the proband were low in general, and the rate of lactate production was low. Kinetic studies using crude hemolysate revealed a normal Km for adenosine, normal electrophoretic mobility but slightly abnormal pH curve and slightly low utilization of 2-deoxyadenosine. The ADA activity of lymphocytes was nearly normal.

Adenosine Deaminase↗
PubMed1978

Establishment and characterization of a human cancer cell line that produces human colony-stimulating factor.

A human colony-stimulating factor (CSF) producing cell line, T3M-1, has been established from explant cultures of a human squamous cell carcinoma of the oral cavity that secretes human CSF. It has been continously propagated during the past 15 months. The cells grew in a monolayered sheet with about 17 hr of population-doubling time and showed a colony-forming capacity with about 5% plating efficiency. The cells exhibited an epithelioid morphology resembling the structure of the original tumor, and they showed "tumor takes" when inoculated into nude mice. Karyotypic analysis revealed the cell line to be a human aneuploid one with a hypotriploid mode, including the Y-chromosome(s) and at least 10 common markers. T3M-1 cells possess the characteristic function of human CSF production in vitro, and a marked neutrophilia was observed in nude mice bearing the tumors produced by inoculation with the T3M-1 represents a new human cell line that secretes human CSF.

Animals↗
PubMed1978

Regeneration of the deep cervical lymphatics--light and electron microscopic observations.

Regeneration of the lymphatic vessels following ligation of the deep cervical lymphatics was studied in 35 rabbits using both light and electron microscopes. The newly formed lymphatics were evident under the electron microscope in the first post-operative week, in which the restoration of the affected endothelial cells of the lymphatics and reaction of the free cells such as fibroblasts and macrophages were observed.

Animals↗