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Biomedical subjects

S Arita

Publications and source records attributed to S Arita.

108 records · Page 6Linked to original sources
PubMed1983

The aetiology and treatment of simple bone cysts.

The internal pressure of simple bone cysts was found to be slightly higher than the normal pressure of the bone marrow in the contralateral limb. The pressure within the cyst was measured during drilling with a Kirschner wire; it gradually decreased as the number of drill-holes increased. The PO2 of the cyst fluid was markedly lower than that of either venous or arterial blood measured synchronously. It is suggested that venous obstruction in the bone is the likely cause of these cysts. Seven patients with simple bone cysts were treated by the multiple drill-hole method, and the clinical outcome was excellent. Multiple drilling may prove to be the treatment of choice for simple bone cysts in the younger patient, as it presents fewer hazards than other procedures.

Adolescent↗
PubMed1983

[Simple bone cyst--pathophysiology and treatment].

Internal pressure of simple bone cyst was slightly higher than normal bone marrow pressure measured on contralateral side. Internal pressure of the cyst measured during drill-holing with Kirschner wire gradually decreased along with the increase in the number of drill-holes. Internal PO2 was markedly lower than PO2 of venous and arterial blood measured synchronously. Venous obstruction in bone may be an etiology of simple bone cyst. Follow up study showed that all cysts of six cases treated by the multiple drill-hole method were filled up with bony tissue within five months after the treatment, and the clinical result of the treatment was excellent. Multiple drill-hole method may be the first choice in the treatment of simple bone cyst in the younger patients, because the operative invasion of this method was minimum.

Adolescent↗
PubMed1983

[Basic characteristics of Gunma clone-4 in the experimental study of metastasis].

Gunma clone-4 (Gc-4) cell was isolated by limiting dilution method from Radiological Science-Chiba- Toyama sarcoma which appeared spontaneously in a C3H/He mouse and cultured for generations. The character of Gc-4 cells was as follows: Gc-4 cells grew exponentially in vivo and in vitro, and the doubling time was 3.4 to 4.2 days in vivo and 20 to 22 hours in vitro respectively. Inoculation of 1 X 10(6) Gc-4 cells into A/Jackson mice and C57BL/6 mice resulted in rejection and disappearance within 17 days. Inoculation of 1 X 10(6) Gc-4 cells into C3H/He mice which were immunized with Gc-4 cells treated with 100 micrograms/ml of mitomycin C for 30 min. did not result in rejection. The Gc-4 cells showed histiocytic and fibroblastic characteristics morphologically and functionally, and corresponded to human malignant fibrous histiocytoma. Experiments on artificial and natural metastasis of Gc-4 cells revealed that Gc-4 cells had a high colony forming efficiency.

Animals↗
PubMed1981

Diagnosis and operative treatment for the brachial plexus injury.

It is shown that the electrophysiological diagnosis of brachial plexus injury is becoming more precise than ever. The intraoperative use of electrodiagnosis especially aids the obtaining of better results for brachial plexus injuries. When nerve transfer is undertaken, biceps function is more usefully restored by the accessory nerve than by the intercostal nerves. Protective sensation was achieved in almost all cases by intercostal nerve transfer. When nerve grafting was undertaken, functional motor restoration was regained in the upper arm muscles in one case, but not in the forearm.

Action Potentials↗
PubMedDate not supplied

Prognostic importance of vascular endothelial growth factor and its receptors in the uterine sarcoma.

Vascular endothelial growth factor (VEGF) and its receptors play an important role in tumor progression; however, there is no report regarding this factor in uterine sarcoma. Thirty-nine patients with uterine sarcoma, 14 carcinosarcomas, 4 endometrial stromal sarcomas, and 21 leiomyosarcomas, were studied. By immunohistochemical staining, VEGF was not detected in normal uterine smooth muscle, but VEGF receptor-1 (flt-1) and VEGF receptor-2 (flk-1) were observed in 14 and 4 of 14 normal smooth muscles, respectively. Of 39 sarcomas, 25 expressed VEGF, and 38 and 34 sarcomas expressed flt-1 and flk-1 at various intensities, respectively. The staining intensity of VEGF, flt-1, and flk-1 was significantly higher in sarcoma than in normal uterine smooth muscle, but that of phospho-flt-1 (p-flt-1) was significantly lower in sarcoma than in normal uterine smooth muscle. When sarcomas were divided into two groups according to staining intensity, a significant difference in survival curves was observed in only p-flt-1 of leiomyosarcoma (P= 0.008), and in all sarcomas, a lower survival curve was also observed in the high staining intensity group than in the low staining intensity group, although there was no significant difference (P= 0.102). In conclusion, VEGF and its receptors are suggested to be involved in progression of uterine sarcoma, but only the p-flt-1 level significantly affected the survival of leiomyosarcoma patients.

Adult↗
PubMedDate not supplied

Correlation between microvessel density and tumor cell proliferation with clinical factors in breast carcinomas.

Fifty-nine methacarn-fixed, paraffin-embedded human breast carcinomas were immunostained by QB-END/10 (an antibody to CD34 antigen) to observe microvessels and by PC10 (an antibody to proliferating cell nuclear antigen; PCNA) to determine tumor cell proliferation; 9 normal human breast tissue specimens were also immunostained by QB-END/10. The number of microvessels in the periphery of the breast carcinoma was significantly greater than both that in the center of the breast carcinoma and that in the normal breast. There was also a significant relationship between the number of microvessels in the periphery of breast carcinomas and the histological tumor size and lymph node status. However, there was no significant relationship between the tumor cell proliferation activity (PCNA positive cell ratio) and any clinical or histopathological variables. The number of microvessels and the tumor cell proliferation activity showed a weak negative correlation.

Adult↗
PubMedDate not supplied

Usefulness of Lens culinaris agglutinin A-reactive fraction of alpha-fetoprotein (AFP-L3) as a marker of distant metastasis from hepatocellular carcinoma.

The objective of this study was to clarify the relationship between the serum level of the Lens culinaris agglutinin A-reactive fraction of alpha-fetoprotein (AFP-L3) and the clinical features including sex, age, Child's classification, virus markers, tumour size, tumour stage, distant metastasis, histopathologic findings, portal thrombus and outcome of hepatocellular carcinoma (HCC). We measured the AFP-L3 levels in 170 HCC cases at the time of diagnosis using lectin-affinity electrophoresis followed by antibody-affinity blotting. The patients were divided into two groups, those who were AFP-L3 positive (n=56; AFP-L3 >/=15% relative to the total alpha-fetoprotein (AFP) concentration) and those who were AFP-L3 negative (n=114; AFP-L3 <15%). Then we examined the association between the serum AFP-L3 level and the clinical features of HCC. No significant differences were found in age, sex, and virus markers between the AFP-L3-positive and -negative groups. However, patients in the positive group had worse liver function and larger tumours compared to the negative group. They also had more advanced cancer with poor tumour histology compared to the negative group. Distant metastasis was diagnosed significantly more often in the positive group than that in the negative group. There was no significant correlation between the AFP-L3 level and portal thrombus. Although the follow-up period was brief the prognosis for the positive group clearly was poor. These results suggest that AFP-L3 is a useful indicator of distant metastasis and a poor prognosis for HCC.

Adult↗
PubMedDate not supplied

Dietary effects of fatty acids on growth and metastasis of KPL-1 human breast cancer cells in vivo and in vitro.

To evaluate the effects of dietary fats on breast cancer growth and metastasis, KPL-1 human breast carcinoma cells which have a propensity for axillary lymph node metastasis when inoculated into the thoracic mammary fat pad of female nude mice were examined. The mice were fed one of three semipurified diets containing 9.5% eicosapentaenoic acid plus 0.5% linoleic acid (EPA diet), 10% linoleic acid (LA diet), or 9.5% palmitic acid plus 0.5% linoleic acid (PA diet), or commercial laboratory chow containing 8.5% fat of which 4.1% was LA, 1.1% was PA, 0.06% was EPA, and 3.24% was other (Standard diet) starting 19 days before tumor cell inoculation and continuing until the end of the experiment (43 days after tumor cell inoculation). The tumor growth was faster and at a higher incidence in the mice fed the LA diet, and much slower and at a lower incidence in the EPA diet group compared with the mice fed the PA or Standard diet; the two separate experiment demonstrated identical results. The differences in tumor weight between the LA and PA groups and between the PA and EPA groups were significant (P < 0.05, respectively) at the termination of the experiment; the differences were due to different tumor cell proliferation rates. In an in vitro MTT assay, fatty acids showed direct stimulatory or inhibitory effects on the KPL-1 cells. Lymph node metastasis was seen in the LA and Standard diet groups, whereas it was not seen in the PA or EPA groups. The body weights were significantly lighter in the LA and EPA groups compared with the PA and Standard diet groups (P < 0.05, respectively). The results indicate that the EPA diet produced a reduction in tumor cell growth and metastasis whereas the LA diet had an enhancing effect on these parameters; dietary fatty acids may thus have a direct role in the growth and metastasis of human breast carcinoma independent of their systemic effects.

Animals↗