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Biomedical subjects

S Akimoto

Publications and source records attributed to S Akimoto.

At least 163 records · Page 9Linked to original sources
PubMed1989

Transcriptional regulation of F plasmid gene srnB: rifampicin-promoted in vitro readthrough of a terminator in the leader region.

srnB is an F-plasmid encoded gene, otherwise silent, whose expression is induced by added rifampicin, leading to the release of cellular Mg2+ and degradation of stable RNA. In the absence of rifampicin, transcripts from the srnB gene were relatively short. S1 nuclease mapping revealed that the short mRNA species terminated within the leader, at the 3' end of a potential stem-and-loop structure. A deletion in the stem-loop resulted in constitutive synthesis of the mRNA that extended beyond the termination site into the structural gene. Even with the wild-type gene, transcription continued beyond the terminator sequence in the presence of added rifampicin. Most of the transcripts synthesized in the presence of rifampicin were long enough to encode the srnB protein. We hypothesize from these results that RNA polymerase associated with rifampicin can read through the terminator to induce srnB expression.

Amino Acid Sequence↗
PubMed1989

[Incidence of temporomandibular joint disorders in patients with malocclusion].

In order to examine the relationship between the incidence of temporomandibular joint (TMJ) disorders and the occlusal problems, epidemiological surveys regarding the signs of TMJ disorders (joint noise, pain and disturbance of mandibular movement) were carried out to 149 untreated patients (54 males and 95 females) with malocclusion. The results indicated as follows: 1) Incidence of TMJ disorders was 33.6% of total samples, 27.8% of males and 36.8% of females. 2) In symptomatic subjects, the most frequent sign of TMJ disorders was joint noise (76.0%), the second was joint pain (68.0%) and the third was disturbance of mandibular movement (38.0%). 3) The incidence of TMJ disorders increased after 8 years of age, while it decreased slightly from 15 to 18 years of age and showed high frequency after 19 years of age. It was suggested that these changes were related with the occlusal configurations such as the exfoliation followed by the eruption of permanent successors or the eruption of the third molars. 4) Symmetrical anterior cross-bite malocclusion showed low incidence of TMJ disorders. In posterior cross-bite malocclusion and mandibular lateral displacement case, however, the signs of TMJ disorders were found frequently. These results suggested that the lateral shift of mandibular head in fossa may relate with the appearance of TMJ disorders.

Adolescent↗
PubMed1989

[Localization of pancreatic insulinomas by combined radiographic methods].

Seventeen cases with a total of eighteen insulinomas were examined by ultrasonography (US), computed tomography (CT), angiography, percutaneous transhepatic portal and pancreatic venous sampling (PTPS) and intraoperative ultrasonography (IOUS). We discussed about diagnostic accuracy of insulinomas on these diagnostic modalities. Tumor localization was achieved by US in 39%, CT in 72% and angiography in 81%. Although total 15 tumors (83%) can be correctly detected, multiple lesions were not completely ruled out. We recommended the criteria in PTPS as follows: Insulin concentration level above 200 mu U/ml. Or, both of insulin concentration ratio (step-up site/control) above three and elevation of the CPR concentration level on the PTPS. According to these criteria, the accuracy rate was 91%. Overall preoperative diagnostic accuracy was 100%. IOUS allowed us to detect the localization of the tumors completely.

Adenoma, Islet Cell↗
PubMed1989

[Morphological feature and incidence of TMJ disorders in mandibular lateral displacement cases].

In the malocclusion with mandibular lateral displacement (MLD), it is difficult to establish the functional occlusion by orthodontic means. The careful diagnosis brings us to recognize that MLD condition is the rule rather than the exception. In order to examine the characteristics of the cranio-facial morphology in the cases with MLD, the posteroanterior cephalograms were analyzed. Furthermore, the incidence of temporomandibular joint (TMJ) disorders (joint sound and joint pain) were examined clinically. The results were as follows: 1) Occlusal plane and mandibular plane (the plane running through the anti-gonialnotch bilaterally) inclined superiorly toward the mandibular displaced side. 2) The position of mandibular head shifted to the opposite direction of the mandibular displaced side. 3) The symptoms of TMJ disorders were found in 65.0% of patients with MLD, and more frequently at the TMJ of mandibular displaced side. Considering the evidences, the symptoms of TMJ disorders were much detected and occlusal deviation was found in MLD, it is suggested that mandibular dysfunctions derived from occlusal problems relate to the appearance of MLD. Since both the mandibular plane and the occlusal plane revealed the similar changes in response to mandibular deviation, it is considered that the condition of MLD as skeletal problem may be induced by the functional lateral shift of mandibular position come from occlusal interference during growth period. The deviation of mandibular head may relate to the appearance of TMJ disorders in MLD. Therefore, it is important to recognize the adequate mandibular position prior to occlusal reconstruction, and the vertical dimension control of the occlusal plane is necessary for improving the condition of TMJ and establishing the functional occlusion in the cases with MLD.

Humans↗
PubMed1989

[Precancerous lesions of the colon and rectum].

Adenomatous polyp, adenomatosis coli and dysplasia in the inflammatory bowel disease are noted as precancerous lesions. 1) Adenomatous polyps are easily found by colonoscopy as protruded lesions and diagnosed by histopathological study. 2) Adenomatosis coli is easily diagnosed by barium enema and colonoscopy. Adenomatosis coli is accompanied with cancer frequently. As soon as adenomatosis coli is detected, we recommend surgery. IRA (total colectomy and ileorectal anastomosis) is mainly undertaken for adenomatosis coli. But when there are many adenomas in the rectum, we recommend IAA (total colectomy, rectal mucosectomy and ileo-anal anastomosis). 3) Diagnosis and therapy of dysplasia accompanied with the inflammatory bowel disease (ulcerative colitis, tuberculosis, Crohn's disease) remain unclear. There is a deep relationship between cancer and dysplasia and inflammation.

Adenoma↗
PubMed1989

[Angiographic findings of gallbladder cancer].

The patients with gallbladder cancer often accompany with chronic cholecystitis. This fact leads angiographic diagnosis difficult. So the angiographic findings of the chronic cholecystitis are first analyzed precisely and subtracting the angiographic findings of the gallbladder cancer from these gives us the pure (true) angiographic findings of the gallbladder cancer. Characteristic angiographic findings of the gallbladder cancer only are stretched cystic artery encasement (27/37 cases) and short straight tumor vessels "bristly vessel" (36/37 cases).

Aged↗
PubMed1989

[The usefulness of two-dimensional transesophageal echocardiography in follow-up studies of dissecting aortic aneurysm].

Two-dimensional transesophageal echocardiography (2D-TEE) facilitates clear imaging of the thoracic aorta, and we have already reported the diagnostic value of this procedure for detecting dissecting aortic aneurysm (DAA). Recently, we performed preoperative and postoperative 2D-TEE in nine surgically treated patients with DAA (Debakey type I: 2 cases, type II: 1 case, type IIIa: 2 cases, type IIIb: 4 cases), and repeated 2D-TEE in six medically treated patients (DeBakey type IIIa: 2 cases, type IIIb: 4 cases), and the usefulness of 2D-TEE in the follow-up study of DAA was evaluated. Two types of ultrasound probe (radial scan type, and sector type with a color-Doppler system) were used in this study. Of the surgically treated patients, four of nine cases in which the initial tears were excised or repaired showed neither leakage nor pseudoaneurysm at the site of graft insertion. Other surgically treated patients showed residual intimal tears and continued to harbor patent false lumen. One patient with type I dissection demonstrated localized saccular aneurysm in the proximal arch of the aorta, but no evidence of aortic regurgitation. Of the six medically treated patients, further extension of the initial dissection was observed in one of the two type IIIa cases. False lumen narrowing was recognizable in three of the four type IIIb patients, and complete obliteration of false lumen was observed at necropsy in one of these three cases. Double-channeled aorta was seen in another case of type IIIb dissection.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1989

[Fast neutron radiotherapy of prostate cancer].

Between 1976 and 1987, sixty eight patients suffering from prostate cancer were treated with fast neutron radiotherapy at the NIRS hospital in Chiba, Japan. Tumor doses of TDF 100 were administered to the prostate gland, the regional lymphnodes, and the true pelvis, using the two opposing fields by fast neutron alone or with a boost. The overall local or regional tumor recurrence rate was found to be 10% and local control seemed to correlate with the histological differentiation of the cancer tissue, though there was no definite correlation with hormone treatment. A relatively high incidence of grade 3 and 4 radiation complications were observed, 21% for grade 3 and 7% for grade 4, due to subcutaneous fat necrosis caused by an inferior dose distribution of the fast neutron machine. The 5-year survival rate was seen to be 52% in total and 70% for patients in stage C. The modality of radiotherapy for prostate cancer is discussed.

Adenocarcinoma↗
PubMed1988

Evaluation of endoscopic ultrasonography for the diagnosis of submucosal tumors of the esophagus.

Endoscopic ultrasonography was carried out on 55 patients whose X-ray films or endoscopic examinations indicated the presence of a submucosal tumor. Endoscopic ultrasonography revealed 8 cases of extraluminal compression and 48 cases of submucosal tumors. Histological studies were performed on 29 cases with submucosal tumors. In 28 of the 29 cases (97%) the location of the tumor in the esophageal wall was correctly estimated ultrasonographically, and appropriate treatment was selected. Tumors ranging from 3 to 50 mm in diameter could be measured accurately. This method may be helpful in follow-up studies. Endoscopic ultrasonographic findings, such as characteristics of the tumor border and internal echoes, were studied to predict the histological diagnosis of the tumor. Leiomyoma, cyst, granular cell tumor, lipoma, and intraluminal metastasis of esophageal cancer were all found to have specific ultrasonographic findings indicating the histological nature of the tumor.

Adult↗
PubMed1988

Effect of leuprolide on growth of rat prostatic tumor (R 3327) and weight of male accessory sex organs.

Leuprolide, a synthetic LHRH analog, inhibited growth of the Dunning R 3327 androgen-sensitive rat prostatic tumor and induced weight loss in male accessory sex organs. The relationship between the mode of administration and efficiency of the treatment was examined. Maintenance of the drug level in vivo seemed to be one of the important factors in the suppression of tumor growth, while a decrease in the weight of the accessory sex organs was mainly dependent on the dose administered. No treatment with leuprolide surpassed the effect caused by castration. Cytosolic androgen receptor and acid phosphatase activity in the tumor tissues were not changed significantly after treatment with leuprolide.

Acid Phosphatase↗
PubMed1988

Characteristics of progestin binder in hypertrophic human prostate.

The human prostate contains a protein which binds with progesterone in a high affinity and low capacity fashion. Characteristics of the progestin-binding protein in the prostate have been disputable; whether it is progesterone receptor or not. Therefore, the characteristics of the progestin binder in the benign hypertrophic human prostate was examined in the present study. After photoaffinity labeling with 3H-R 5020, the binder in the prostate migrated to the site of 42K on polyacrylamide gel electrophoresis under denaturing conditions, and the mobility was apparently different from that of the progesterone receptor in the human uterine endometrium. There was no protein in the prostate immunoreacted with a monoclonal antibody raised against the human progesterone receptor. It was concluded that the progestin-binding protein in the human prostate was different from the progesterone receptor observed in the female human organs.

Blotting, Western↗
PubMed1988

[Prostate specific antigen in serum of the patients with prostatic cancer].

The serum prostate specific antigen (PA) was determined with the Diagnostic Products Cooperation (DPC) PSA double antibody radioimmunoassay kit. The upper limit of the normal range was set at 4 ng/ml which was the mean + 3S.D. for males over 50 years old in a mass examination. For comparison, prostatic acid phosphatase (PAP), and gamma-seminoprotein (gamma-Sm) were determined using an Eiken kit and Chugai kit, and PA was also assayed using another kit (Eiken, Travenol). Positive rate of PA and PAP in the untreated prostatic cancer was 75 and 33% in Stage A, 100 and 0% in Stage B, 100 and 100% in Stage C, 100 and 67% in Stage D1, 100 and 80% in Stage D2 and 73 and 33% in benign prostatic hypertrophy (BPH), respectively. The level of PA determined during the follow-up of prostatic cancer showed the usefulness of simultaneous PA and PAP assays for monitoring the clinical course. The PA level using a DPC kit was highly correlated to that of PA using other kit, but the correlation with gamma-Sm and PAP was low. These results show that the DPC kit is useful for determining PA, and determination of PA and PAP is of great value both in diagnosis and in the follow-up of prostatic cancer, but the high positive rate in BPH remains a problem.

Acid Phosphatase↗
PubMed1988

[Prostatic acid phosphatase (PAP), gamma-seminoprotein (gamma-Sm) and prostate specific antigen (PA) in prostatic cancer].

The levels of prostatic acid phosphatase (PAP), gamma-seminoprotein (gamma-Sm) and prostate specific antigen (PA) were determined in the serum of 200 untreated patients 28 patients with reactivated prostatic cancer and 179 patients with benign prostatic hypertrophy (BPH) from 1979 to 1987. PAP and gamma-Sm were determined using an Eiken and Chugai kit, respectively and PA was assayed using an Eiken or Travenol kit. The sensitivity of PAP, gamma-Sm and PA respectively in the untreated prostatic cancer cases was 0, 0% and 67%, for Stage A1, 25, 17 and 100% for Stage A2, 23, 50 and 60% for Stage B, 62, 81 and 94% in Stage C, 58, 67 and 90% for Stage D1, 86, 88 and 100% for Stage D2. The specificity of PAP, gamma-Sm and PA is 89, 69 and 43%, respectively. The efficiency of PAP was the highest at all stages as a whole, but when compared at each stage, gamma-Sm was the highest at Stages B and C. The sensitivity of simultaneous assays of PAP and gamma-Sm was slightly increased, but sensitivity was not increased by simultaneous use of three markers. The efficiency of a simultaneous assay was lower than that of a single assay with PAP. However, combined determination of gamma-Sm or PA with PAP was found to be useful for monitoring the clinical course of the reactivated patients. Correlation between PAP and PA levels was high, but that between gamma-Sm and PA levels was low. There was no correlation between PAP and gamma-Sm levels. In conclusion, PAP is the most valuable marker for prostatic cancer, and gamma-Sm is of value for use in combination with PAP. However, an additional PA assay was not found to be of advantage.

Acid Phosphatase↗
PubMed1988

[Comparison of 3 assay kits of prostate specific antigen in serum of prostatic cancer].

The serum prostate specific antigen (PA) of the patients with prostatic cancer were determined with 3 assay kits, the Diagnostic Products Cooperation (DPC) kit, the Eiken kit and the Dainippon Pharmaceutical Co. (MARKIT F) kit. The first 2 assay kits involve radioimmunoassay and the latter EIA. For comparison, prostatic acid phosphatase (PAP) and gamma-seminoprotein (gamma-Sm) were determined using an Eiken kit and Chugai kit. Efficiency of the DPC kit, Eiken kit and the MARKIT F kit for untreated prostatic cancer was 26, 25 and 36%, respectively. The PA level measured using the Eiken kit and the MARKIT F kit both well correlated to the PAP level, but with the DPC kit correlation was slightly low. The PA level measured using the 3 different kits correlated poorly with the gamma-Sm level. The PA values obtained with 3 different assays from patients with prostatic cancer were highly correlated, but showed great differences in the values measured. When the standards used in the DPC kit were analyzed by the Eiken kit, the DPC standards as measured by the Eiken kit had only about half of their assigned values. The same standards were analyzed by the MARKIT F kit, the standards yielded measured values about one third of their assigned values. When the standards used in the MARKIT F kit were analyzed by the Eiken kit, the MARKIT F standards yielded measured values about 2.5 fold of their assigned values. The differences between the values obtained with the 3 assay kits presented a serious problem in clinical use of PA. Standardization of these assay kits will be awaited.

Acid Phosphatase↗