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Biomedical subjects

S A Stass

Publications and source records attributed to S A Stass.

At least 109 records · Page 6Linked to original sources
PubMed1981

Terminal transferase in acute lymphoblast leukemia in remission.

Terminal deoxynucleotidyl transferase (TdT) is a marker for the diagnosis of acute lymphoblastic leukemia. To determine its value as an indicator of bone marrow remission or impending relapse, serial remission marrows (less than 5% blasts) from 49 patients who had acute lymphoblastic leukemia were examined for TdT by immunofluorescence over the period of a year. Thirty-eight patients (78%) had less than 1% TdT-positive cells. Eleven patients (22%) had slightly elevated levels of TdT (2%-7%) at some time during the study. of these eleven, only two had relapses; however, neither patient had greater than 1% TdT-positive cells within the three months before the relapse. Therefore, it appears that the presence of slightly increased numbers of TdT-positive cells in acute lymphoblastic leukemia remission bone marrows (2%-7%) does not denote impending relapse. In addition, most of the patients with acute lymphoblastic leukemia in remission had less than 1% TdT-positive cells.

Acute Disease↗
PubMed1981

Pre-T chronic lymphocytic leukemia with common acute lymphoblastic leukemia antigen. A new immunologic variant.

Chronic lymphocytic leukemia (CLL) is a B cell disorder but occasionally may have the characteristics of a T cell and rarely as a dual T and B cell. A case of pre- T cell and rarely as a dual T and B cell. A case of pre-T CLL with common acute lymphoblastic leukemia antigens is presented. The markers Studies of the bone marrow lymphocytes were +HTLA, +cALL, +EAC, +Ia, -TdT, -E and peripheral blood lymphocytes were +HTLA, +Ia, -cAll, -EAC, -TdT, -E. The poor course of our patient suggests that more detail subtyping of CLL to identify similar subtype may provide additional prognostic information.

Antigens, Neoplasm↗
PubMed1981

Pseudoleukemia. Ineffective regulation of granulopoiesis.

A neonate with Down's syndrome confirmed by chromosomal analysis had leukocytosis, with a WBC count of 48,000/cu mm, at age 13 days. Differential counts showed 70% to 90% blast forms by light microscopy. The blast-cell granules stained negatively with Sudan black B and alpha-napthyl butyrate esterase. Myeloperoxidase activity evaluated light and electron microscopy was negative. The granules stained positively with PAS, beta-glucuronidase, and tartrate-sensitive acid phosphatase. Indirect immunofluorescence for terminal-deoxynucleotidyl-transferase was negative. Our results show that the blast cells of pseudoleukemia may be of lymphoid origin.

Bone Marrow Cells↗
PubMed1980

Value of imprint preparations of bone marrow biopsies in hematologic diagnosis.

Imprint preparations were performed on bone marrow biopsy specimens obtained from the posterior superior iliac spine of 310 consecutive patients. Bone marrow aspiration was attempted prior to biopsy. When aspiration was impossible or inadequate, imprint preparations were used in order to study cytologic detail and enhance interpretation of the biopsy specimen. Malignant infiltrates were present in 22 (7.1%) of the 310 biopsy specimens including: non-Hodgkin's lymphoma, 12 patients, metastatic carcinoma, 6; Hodgkin's disease, 1; multiple myeloma, 1; Waldenstrom's macroglobulinemia, 1; and Sezary's syndrome, 1. An aspirate could not be obtained in 8 (36%) of 22 cases, but malignant cells were found in imprint preparations of the biopsy specimens. This study demonstrates that imprint preparations of bone marrow biopsies are useful in evaluating patients with malignancy when bone marrow aspiration is inadequate.

Adolescent↗
PubMed1980

Immunoassay of circulating terminal transferase in patients with acute lymphoblastic leukemia: a new technique for diagnosis.

The measurement of circulating terminal deoxynucleotidyl transferase (TdT) antigen can be accomplished by the use of an ELISA immunoassay system. TdT was present in either peripheral or bone marrow plasma of five patients with acute lymphoblastic leukemia (ALL) and absent from normal controls using ELISA. The assay was sensitive to a level of 20 ng/ml. The ALL patient values ranged from 160 ng/ml to 420 ng/ml. Use of ELISA for quantitation of TdT may have important diagnostic implications in patients with ALL.

Bone Marrow↗
PubMed1980

Pappenheimer bodies. An additional cause for a spurious platelet count.

A 67-year-old woman who had sideroblastic anemia had a spuriously elevated platelet count due primarily to Pappenheimer bodies. The platelet count on an electrooptical instrument was falsely elevated. The true platelet count was determined by phase microscopy using lysed whole blood and by the peripheral blood smear. There appear to have been no previous reports of falsely elevated platelet counts due to Pappenheimer bodies. Other causes of spuriously elevated platelet counts are reviewed. The reasons for the spurious counts are discussed.

Aged↗
PubMed1980

Acute lymphoblastic leukemia: hand mirror variant.

The presence of numerous hand mirror cells in the bone marrow of patients with acute leukemia may be related to a good prognosis. To further evaluate this relationship, the bone marrows of 21 consecutive patients with a diagnosis of acute lymphoblastic leukemia were reviewed for the presence of greater than 40% hand mirror cells. Four of these 21 patients (19%) demonstrated this finding during the course of their illness. These patients were characterized by ease of remission and/or prolonged survival. Three (75%) patients are alive and well at the time of this writing. At the present time, of the 17 patients without hand mirror cells, seven (41%) are alive and ten (59%) are dead. Although not statistically significant, our findings suggest a positive relationship between the presence of greater than 40% hand mirror cells in the bone marrow ans a favorable prognosis. Future larger prospective studies are indicated to further understand the importance of this complex, apparently immunologically stimulated cell.

Adult↗
PubMed1980

Antigen--antibody complexes related to the baboon endogenous virus in humans with acute lymphoblastic leukemia--hand mirror variant (ALL-HMC).

Hand mirror cells are a morphological configuration that are seen in immunologically stimulated lymphocytes and can be induced by antigen--antibody complexes. Therefore, the bone marrow and peripheral blood plasma of two patients with acute lymphoblastic leukemia--hand mirror variant were evaluated for the presence of antigen--antibody complexes. Both patients had antigen--antibody complexes in the bone marrow plasma and not in the peripheral blood plasma as determined by double counter-current immunoelectrophoresis. The antigen moiety of these complexes appears immunologically related to components of the baboon endogenous virus (BaEV), and the antibody moiety also appears related to structural components of the BaEV. Bone marrow plasmas from patients without leukemia were evaluated for the presence of antigen--antibody complexes and found to be negative. The antigen--antibody complexes may account for the presence of hand mirror cells in the bone marrow of patients with acute lymphoblastic leukemia--hand mirror variant.

Acute Disease↗
PubMed1980

Hand-mirror lymphocytes in infectious mononucleosis.

The identification of a morphologically unique lymphocyte in the peripheral blood of patients with infectious mononucleosis (IM) led to additional studies. Examination of Wright-stained smears of EDTA anticoagulated blood obtained from 25 patients with IM at presentation revealed significantly increased (p less than 0.0001) percentages of hand-mirror lymphocytes (HML) (mean 9.2%) compared with normal controls (mean 2.7%) or controls with nonspecific viral syndromes (mean 2.2%). Follow-up blood samples obtained on 10 of these patients demonstrated a marked increase in the HML count (mean 12.1%) that coincided with the onset of recovery. E-rosette separation of Ficoll-Hypaque-derived peripheral blood lymphocytes from 5 patients with early recovery IM showed the HML to be present almost exclusively in the T-cell population, representing about 25% of the T cells. Identical procedures on 5 controls showed less than 5% HML in the T-cell sample. Cytochemistries supported a T-cell derivation for HML. Electron microscopic examination of HML in IM demonstrated that these cells have unique ultrastructural features that may be related to functions of cellular attachment and cytotoxicity.

Humans↗
PubMed1979

Acute lymphoblastic leukemia--hand mirror variant. An analysis of a large group of patients.

One hundred and thirty-four initial bone marrows and 102 peripheral blood smears were evaluated for hand mirror cells (HMC) on pediatric patients with acute lymphoblastic leukemia (ALL). Twenty (15%) of the 134 patients were found to have greater than 5% HMC in their bone marrows. Only rarely were a few HMC noted in the peripheral blood. Survival did not appear to be related to the number of HMC in the bone marrow. Also, the median survival was not significantly greater in those HMC patients with greater than 40% HMC in their bone marrows when compared to the non-HMC group. From the available data in this study, it would appear that the number of HMC does not affect prognosis in the bone marrows of children with ALL. However, the prognostic significance of the HMC has not been clearly established in adults.

Adolescent↗