Human cytomegalovirus was capable of adsorbing to and penetrating guinea pig cells, but was unable to replicate new virus. Cultures infected with virus inoculum of high titer showed a cytopathic effect (CPE) characterized by cell rounding. This CPE depended upon the presence of infectious virus, and its extent was directly related to the multiplicity of infection. Staining by indirect immunofluorescence by using human convalescent sera was positive as early as 4 h postinfection. Maximal fluorescence was observed 24 h postinfection when 50% of the cells contained fluorescent antigens both in nuclei and cytoplasm. No evidence for viral replication was found, and no defective particles were detected by electron microscopy. Treatment with actinomycin D or with cycloheximide strongly inhibited both the fluorescent antigens and the CPE, whereas 5-fluorodeoxyuridine and bromodeoxyuridine were ineffective.

Animals↗

PubMed1973

Extraintestinal shigellosis: bacteremia and paroxysmal atrial tachycardia. Shigella sonnei in a three-year-old boy.

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Child, Preschool↗

PubMed1973

Antibiotics 1973.

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Aminoglycosides↗

PubMed1972

Fundamentalist and eschatologic approach to ethics.

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Christianity↗

PubMed1972

Candidal osteomyelitis and arthritis in a neonate.

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Amphotericin B↗

PubMed1972

Indirect hemagglutination test for varicella-zoster infection.

An indirect hemagglutination test has been adapted for use with varicella-zoster serology. Antigen was made in infected WI-38 human diploid cell line. The indirect hemagglutination antigen seemed to be a subunit of the virus particle. The antibodies measured in the test are well correlated with neutralizing and complement-fixing antibodies in cases of zoster but not in cases of varicella.

Antibodies, Viral↗

PubMed1972