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Biomedical subjects

R Zaizov

Publications and source records attributed to R Zaizov.

At least 145 records · Page 8Linked to original sources
PubMed1983

Phenotypic characterization of acute leukemia with monoclonal antibodies using the microlymphocytotoxicity assay.

Surface antigens of lymphoblasts from 56 pediatric ALL patients were studied with a set of complement fixing monoclonal antibodies. This group of lymphoblasts was comprised of 22 T-cell ALL, 22 CALLA+ Ia+ ALL and 12 non-T-non-B, CALLA- Ia+ ALL. For comparison, two adult T-cell CLL and six B-cell CLL were also studied. It was found that by using the microlymphocytotoxicity technique, the lymphoblasts can be assigned their immunophenotype and thus be classified into their respective lineage and stage of differentiation. In the samples tested, concordant reactivity was observed when FACS fluorescence profile was compared with that of microlymphocytotoxicity suggesting that the latter can be used especially when qualitative estimates are required.

Acute Disease↗
PubMed1983

Systemic lupus erythematosus and lymphoma. A family study.

After 3 years of treatment with azathioprine and prednisone, immunoblastic lymphoma appeared in a patient with systemic lupus erythematosus. An increased incidence of immunological aberrations and malignancies was found in her family and their relation to the pathogenesis of this patient's diseases is discussed.

Adult↗
PubMed1983

Clinical and virological observations in childhood Hodgkin's disease in Israel.

Antibody levels to Epstein-Barr virus (EBV), cytomegalovirus (CMV), herpes simplex virus (HSV) and varicella-zoster virus (VZV) were determined in children with Hodgkin's disease (HD) before therapy, as well as in healthy, age-matched controls. Included were 21 Israeli-born children, aged 2.5 to 14.5 years: 13 Jews whose parents were of African-Asian origin, 1 Jew whose parents were of European origin, and 7 Arabs. Most of them were from large families (average 5.5 children per family) and low socioeconomic class. Antibody levels were not statistically different between patient and control groups with respect to CMV, HSV and VZV. Geometric mean titer of antibody to EBV viral capsid antigen in HD patients was 100.0 compared with 10.8 in controls. Thirty-three percent of controls were seronegative to EBV, and none had titers greater than or equal to 1:160. All patients but one were seropositive to EBV, and 8 of 20 had titers greater than or equal to 1:160. Among patients, 13 experienced onset of symptoms in the cold season (October to March) and 8 in the hot season (April to September). Onset during the cold season was usually abrupt with acute symptoms, Stage III to IV, involving the mediastinum and neck. Onset during the hot season was insidious, Stage I to II, with the affected area frequently located below the diaphragm. It is suggested that children of Arab and African-Asian Jewish origin with high serum titers to EBV are at increased risk for HD and for seasonal onset-associated clinical presentation.

Adolescent↗
PubMed1981

Suppressor mononuclear cells in giant lymph node hyperplasia and thymoma.

The existence of suppressor mononuclear cells were demonstrated in two lymphoproliferative disorders: giant lymph node hyperplasia and thymona. The three patients tested also showed cell-mediated immunodeficiency as expressed in the low number of T cells, negative graft versus host reactions and negative skin tests; the one patient tested with phytohemagglutinin (PHA) and concavalin A (Con A) showed a low response. Suppressor activity was tested with a new experimental model, the local xenogeneic graft versus host reaction. It is proposed that this model be used for testing suppressor activities in other human disorders as well.

Adolescent↗
PubMed1981

Juvenile and adult types of chronic granulocytic leukemia of childhood: growth patterns and characteristics of granulocyte-macrophage colony forming cells.

Peripheral blood and bone marrow cells from three children with the juvenile (Ph1 negative) type of chronic granulocytic leukemia and from one with the adult (Ph1 positive) type were cultured in soft agar, and their specific growth patterns were evaluated. Greatly increased numbers of colonies were obtained in all cases, particularly from peripheral blood cells. By morphologic, cytochemical and ultrastructural criteria, colonies from one juvenile type and from the single adult type patients were found to be almost exclusively granulocytic, whereas in the other two juvenile type leukemia patients colonies were either granulocytic or macrophage. Moreover, both growth patterns were obtained in the same patients on different occasions. It appears that the leukemic cell populations of the juvenile and the adult forms of chronic granulocytic leukemia do not arise from different cell lines. Rather, both are the progeny of the common monocyte-granulocyte progenitor cell, whose abnormal proliferation and differentiation along either the granulocytic or the monocytic pathway is probably directed by fluctuations in humoral and/or microenvironmental factors.

Bone Marrow↗
PubMed1980

Establishment and characterization of a new leukaemic T-cell line (Peer) with an unusual phenotype.

We report the isolation and establishment in continuous culture of a human lymphoid cell line (Peer) from a case of T-leukemia. The Peer cell line lacks some typical cell-surface properties of T cells, namely sheep erythrocyte rosette formation and reactivity with two anti-T-cell sera, but has focal acid phosphatase and does express two other T-cell antigens, one defined by a monoclonal antibody, the other related to a T-cell subset (TH2). The cells are negative for B-cell markers (SmIg or cytoplasmic mu Fcgamma and C3 receptors, mouse erythrocyte rosettes) and EBV (EBNA). In addition, the Peer cell does not possess the typical phenotypic markers of "non-B, non-T" leukemia: cALL and Ia-like antigens, and the cytoplasmic hexosaminidase isoenzyme I, but is positive for terminal deoxynucleotidyl transferase by enzymatic and immunofluorescent criteria. The cell line requires exogenous L-asparagine for adequate growth in culture, a property known to be characteristic of certain T cells but not of B cells. The Peer cell line appears to have a maturation arrest at a developmental stage intermediate between the cortical thymocyte and a mature T-cell subset and to have lost some T-cell differentiation features.

Antigens, Neoplasm↗
PubMed1980

Balanced bone marrow globin synthesis in mideastern alpha-thalassemia.

Hematological data and globin synthesis studies in 8 alpha-thalassemic children whose parents are from various regions in the Middle East are reported. All patients were devoid of Hb H. 5 of them had mild anemia, hypochromia and microcytosis and their blood alpha-/non-alpha-globin radioactivity ratios ranged between 0.56 and 0.75. The other 3 patients were hematologically normal with blood radioactivity ratios between 0.77 and 0.88. Bone marrow studies showed balanced globin chain synthesis for 6 of the patients and relatively more alpha-globin synthesis than in the blood for the remaining 2. The decrease in the relative synthesis of alpha-globin on erythroid cell maturation may relate to the molecular basis of alpha-thalassemia in the Middle East.

Bone Marrow↗
PubMed1979

Pulmonary alveolar proteinosis associated with fanconi's anemia.

A 16-year old patient with Fanconi's anemia developed pre-leukemia and pulmonary infiltration which was found upon autopsy to be pulmonary alveolar proteinosis. The question whether the pulmonary alveolar proteinosis was due to the marked leukopenia and an eventual defective phagocytic ability of the leukocytes is raised.

Adolescent↗
PubMed1979

Myeloproliferative disease of childhood associated with a trisomy 21 clone.

Myeloproliferative disease of childhood is frequently associated with chromosomal anomalies, usually of the C group. Clinical features are similar to those of the juvenile type of chronic myeloid leukemia. A child with this disease is described. Marked myeloid proliferation, anemia, thrombocytopenia and hepatosplenomegaly were present; leukocyte alkaline phosphatase and fetal hemoglobin were moderately elevated. Chromosome analysis of bone marrow cells revealed a mosaicism 47,XX,+21/46,XX. Down's syndrome was ruled out by the child's normal phenotype and dermatoglyphic analysis. The cytogenetic finding is probably evidence for the clonal origin of the trisomy 21 cell line.

Bone Marrow Cells↗
PubMed1978

Effect of intrauterine growth on erythropoiesis.

Erythropoietic activity, as reflected in the reticulocyte count and the incorporation of 59Fe into red cells, was studied in relation to intrauterine growth of rabbit fetuses during the last third of the period of gestation. Radioiron (10 microci) was administered by i.v. injection into the mothers and placental transfer to the fetuses. The fetuses were extracted after 48 hours. The amount of radioiron received by each individual fetus was determined by whole body counting. Radioactivity in red cells was determined and calculated as a percentage of the dose received. From day 21 to day 31 of gestation, the hemoglobin concentration increased by a factor of 1.5, from 8 to 12.2 g/100 ml, while the body mass increased by a factor of 7, from 6.6 to 47.5 g. During the same period the rate of growth declined from 45 to 20% in 24 hours. This was paralleled by a drop in 59Fe incorporation from 35 to 12% and in the reticulocyte count from 85 to 14.5%. It is concluded that the rate of erythropoiesis in fetal life is largely controlled by the rate of growth and the corresponding increase in hemoglobin mass.

Animals↗
PubMed1978

Globin-chain synthesis in Hb H disease: the activity of red cell precursors and their mRNA.

Globin-chain synthesis was determined in bone marrow and blood of four patients with Hb H disease and in a cell-free system supplemented with poly(A)-rich RNA from the patients. The relative synthesis of alpha-globin ranged between 0.36 and 0.49 in erythroid precursors and between 0.09 and 0.28 in reticulocytes. The relative quantity of biologically active alpha-globin mRNA in poly(A)-rich RNA from bone marrow and blood of these patients ranged between 0.09 and 0.21. The findings suggest the operation, within alpha-thalassemic bone marrow cells, of a translational control mechanism and/or the instability of alpha-globin mRNA.

Bone Marrow↗