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Biomedical subjects

R Wolf

Publications and source records attributed to R Wolf.

At least 271 records · Page 15Linked to original sources
PubMed1985

Pityriasis rosea and ketotifen.

A 4-year-old female patient who developed a skin eruption similar to pityriasis rosea after treatment with ketotifen (Zaditen) is presented. The relationship between ketotifen and the eruption has been based on circumstantial evidence and confirmed by the positive results of the MIF test and the rat mast cell degranulation test.

Bronchitis↗
PubMed1985

Mycosis fungoides-like lesions associated with phenytoin therapy.

An 83-year-old woman developed generalized lymph node hyperplasia together with mycosis fungoides-like skin lesions 11 months after institution of anticonvulsant therapy with phenytoin. The clinicopathological changes disappeared completely three weeks after cessation of therapy. This circumstantial evidence together with the known data on the association of phenytoin with lymphoproliferative disorders suggest that it was the phenytoin that was responsible for the patient's condition. This case had features similar to those associated with the pseudo-mycosis fungoides syndrome except that the cutaneous lesions consisted of two localized erythematous plaques, with no generalized exfoliative erythrodermic dermatitis. Nonetheless, we believe that the use of the term pseudo-mycosis fungoides for this and similar cases would be appropriate.

Aged↗
PubMed1984

Biophoton emission. New evidence for coherence and DNA as source.

The phenomenon of ultraweak photon emission from living systems was further investigated in order to elucidate the physical properties of this radiation and its possible source. We obtained evidence that the light has a high degree of coherence because of (1) its photon count statistics, (2) its spectral distribution, (3) its decay behavior after exposure to light illumination, and (4) its transparency through optically thick materials. Moreover, DNA is apparently at least an important source, since conformational changes induced with ethidium bromide in vivo are clearly reflected by changes of the photon emission of cells. The physical properties of the radiation are described, taking DNA as an exciplex laser system, where a stable state can be reached far from thermal equilibrium at threshold.

DNA↗
PubMed1984

The role of IgE in atopic diseases.

The fact that about 20% of the patients with severe atopic disease have normal or subnormal serum IgE levels, and that the severity of the disease does not always correlate with IgE levels, does not reduce the importance of this antibody in the onset of the illness. A possibility is suggested here that even low IgE concentrations are capable of playing a key role in the pathogenesis of the disease, and being directly responsible for its clinical manifestations. Namely, atopic patients who show low IgE levels seem to have high tissue sensitivity even to these low levels of IgE and to react with these low concentrations of the antibody.

Animals↗
PubMed1984

[Dose measurements in determining radiation exposure in mammography].

Phantom dose measurements were carried out at a mammography unit-type Siemens Mammomat--using LiF thermoluminescence dosimeters. Full calculative corrections were accomplished for the difference between the elementary composition of the phantom material and the human breast. As the results show, the dose required is smaller by about the factor 6 compared to techniques not using intensifying screens. The mean dose lies between 0.07 and 0.31 rd per X-ray picture.

Breast Neoplasms↗
PubMed1984

The clinical pharmacokinetics and tolerance of enoxacin in healthy volunteers.

In a single-dose tolerance and pharmacokinetics study, enoxacin doses ranging from 200 to 1600 mg were administered orally to 12 healthy normal volunteers. Plasma assays demonstrated rapid absorption of enoxacin with first-order elimination and a half-life averaging 3.4-6.4 h. Renal clearance accounted for approximately 40% of total body clearance of drug. In a second placebo-controlled study, 18 normal volunteers received enoxacin in doses of 400, 600 or 800 mg twice daily for 14 days. Plasma concentrations and pharmacokinetic parameters obtained after the first dose were not significantly different from those observed in the single-dose study. With repeated administration, steady-state plasma concentrations were achieved in three days or less. Steady-state pharmacokinetics were characterized by prompt absorption, first-order elimination, and high urinary concentrations of enoxacin. The most frequently-reported adverse experiences involved the gastro-intestinal tract, the central nervous system, and the skin.

Clinical Trials as Topic↗
PubMed1984

Growth and tolerance studies of a new infant formula.

A new routine infant formula has been developed and clinically tested. The clinical study reported here involved 337 normal newborns cared for by six private pediatric group practices. Infants were examined regularly by the investigators until six months of age. Serum biochemistries, hematology, and growth and tolerance variables were compared to infants fed control formulas and to reported values for breast-fed infants. This new formulation compared favorably, in all areas studied here, to controls and previously reported values.

Blood Proteins↗
PubMed1984

Bowel bypass arthritis dermatitis syndrome: a histological and electron microscopical study.

A case of dermal vasculitis with arthralgia after intestinal bypass surgery is reported. A 36-year-old woman developed arthralgia and skin rash, 1 year after an ileo-jejunal bypass operation was performed for overweight (130 kg). Skin biopsy showed leucocytoclastic vasculitis in the dermis. E.M. study showed clumps of platelets around small dermis blood vessels, and polymorphous perivascular infiltration. The symptoms subsided after tetracycline treatment.

Adult↗
PubMed1984

RNA and DNA content of isolated nuclei from bladder irrigation specimens as measured by flow cytometry.

Cell nuclei were isolated from bladder irrigation specimens from urologic patients having neoplastic disease and distinct aneuploid tumor populations with ploidy levels greater than 2. 0c . The isolated nuclei were subsequently stained with acridine orange, and their fluorescence was measured by flow cytometry. The RNA-DNA frequency distributions of nuclei were compared to those of whole, intact cells from the identical specimens. A comparison of RNA content revealed distinct subpopulations of aneuploid G1 tumor cells with different RNA content. The low and high RNA subpopulations were present in both whole cells and intact nuclei. The overall profile regarding ploidy levels of tumors as well as cell cycle distributions were comparable in both whole cells and nuclei. The resolution of DNA content, however, was significantly better in nuclei preparations. This fact made it easier to distinguish aneuploid cells with near-diploid values of DNA content.

Aneuploidy↗
PubMed1984

Concurrent cell-mediated immunity and immediate hypersensitivity in animal model.

Drug induced allergic reactions in treated patients are sometimes accompanied by laboratory evidence of a simultaneous drug-specific humoral and cellular immunity. The relationship between the two types of immune response was studied in an experimental model. In the present studies ICR mice were sensitized with horse serum to induce an anaphylactic shock. Macrophage migration inhibition factor (MIF) test, which is an in vitro correlate for cell-mediated immunity (CMI), was performed with the animal's lymphocytes against horse serum. A significant inhibition in the macrophage migration was observed in the sensitized mice as compared to the control group. No significant difference was observed between the migration index of the mice that suffered fatal anaphylactic shock and those with milder symptoms that survived it. The demonstration of a positive MIF in an immediate type allergic reaction does not necessarily indicate a direct involvement of the CMI system in the production of clinical manifestations, although the MIF test may be clinically useful for diagnostic purposes, even in immediate hypersensitivity cases.

Animals↗
PubMed1984

Positive intradermal tests to D-tubocurarine in patients with cholinergic urticaria.

Patients with cholinergic urticaria exhibit increased hypersensitivity to cholinergic drugs. In the present study, an attempt was made to determine whether these patients would also cross-react to various neuromuscular blocking agents having structures analogous with acetylcholine. The four patients tested showed positive skin tests for D-tubocurarine at concentrations of 1/10,000. None of the 10 subjects in the control group and none of the patients with urticaria had positive responses, not even to a 10-fold higher concentration of D-tubocurarine. These findings suggest that caution should be exercised in the use of neuromuscular blocking agents in patients with cholinergic urticaria.

Adolescent↗
PubMed1983

Fusion resistance and decreased infectability as major host cell determinants of coronavirus persistence.

Mouse hepatitis virus persists in cultures of a subline (designated LM-K) of mouse LM cells but produces a lytic infection in L-2 cells. Persistence in the LM-K cells was not accompanied by production of ts mutants or of soluble anti-MHV factors. Infectious center assay demonstrated an approximately 500-fold lower level of infectibility by MHV of the LM-K cells as compared to L-2 cells. On an infected cell basis, production levels of infectious progeny and viral RNA were comparable between the two cell lines. The extent of virus-induced cell-cell fusion, however, was markedly reduced in the LM-K cells. Cell-mixing experiments showed that both infected L-2 and LM-K cells have the capacity of fusing with neighboring uninfected L-2 cells but not with uninfected LM-K cells. This suggests that the decreased level of fusion observed in the LM-K infection is due not to absence of viral fusion protein at the cell surface, but rather to an inherent resistance of the LM-K cell membrane to MHV-induced fusion. It is believed that such fusion resistance in LM-K cells moderates virus dissemination throughout the culture, thereby contributing to a state of virus persistence.

Animals↗
PubMed1983

Minor aesthetic blemishes: the patient's unasked request.

This paper describes a case representative of a group of people who are troubled by cosmetic blemishes but are unable to verbalize their feelings. After appropriate treatment, they are able to verbalize their feelings and they feel great relief.

Adult↗