[Letter: Negative experience with the perfusor].
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Biomedical subjects
Publications and source records attributed to R Voss.
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The lymphocytes of a patient with leukemic lymphosarcoma were found to have an unusual surface phenotype in that they bound both sheep erythrocytes (a T cell marker) and complement-coated erythrocytes (a B cell marker) but lacked other B cell surface characteristics. Marker chromosomes were present in these cells, but not in other, phenotypically normal cells from the same patient. This case may provide a clue to the chromosomal origin of some lymphocyte surface markers in man.
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In man-mouse hybrid line from our cell library, the only cytological detectable portion of the human genome is the X chromosome, and the only genetic markers regularly expressed are coded by genes known to be ?X-linked. A component of the heterogeneous nuclear RNA of these cells was found to be complementary to repetitive human DNA sequences by means of RNA-DNA hybridization on nitrocellulose filters. The same procedure also permitted the identification of hybrid cell DNA sequences that are complementary to human heterogeneous nuclear RNA. This experimental approach, coupled with hybridization studies in situ, is expected to yield critical data on the distribution and the specificity of the repetitive DNA sequences present in the human genome and to provide a new tool for cytological mapping of human chromosomes.
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In cases of deep bite where the lower front teeth contact the palatal gingiva or where the upper teeth contact the labial gingiva of the lower jaw, the attempt to improve the situation by grinding-in the teeth was always crowned with temporary success only. The question as to whether the front teeth may be maintained determines further measures to be taken. If they can and if they are to be kept it is necessary to change the vertical relation of practically all teeth by safeguarding the antagonistic contract also in the front area. Therapeutical means and risks involved are considerable. If the teeth concerned have to be removed, plastic correction of the alveolar ridge is necessary before bridges and removable dentures are fitted in. This solution is even less satisfactory and disregards the significance of natural teeth for the well-being of the patient.
It is reported on a data processing system with computer dialogue for the "standardized sheet of findings". The computer-readable form, improved error control, and optimum evaluation possibilities are emphasized as special advantages. Simple handling and optimum data protection permit to have this system operated by trained laymen.
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