Biomedical subjects

R Umeda

Publications and source records attributed to R Umeda.

At least 37 records · Page 2Linked to original sources

PubMed1989

Electro-olfactogram (EOG) in olfactometry.

The electro-olfactogram (EOG) is a potential originated in olfactory epithelium evoked by odorous stimulation, which has been demonstrated and termed by Ottoson (1959). In order to ascertain the clinical application of EOG, we attempted to record similar potentials in human subjects. In some preliminary experiments using rabbits, it was quite easy to produce Ottoson's findings. In human subjects, however, there were sometimes great technical difficulties owing to the hidden anatomical position of the olfactory epithelium. Then we utilized Olympus selfoscope endoscopy for placing and retaining the electrode to the olfactory portion precisely and safely, with a tube for sending airborne odor. As results, we succeeded in recording negative spike discharge similar to Ottoson's findings. We examined a total of 27 nasal cavities in 22 patients with peripheral olfactory disorders and 11 nasal cavities in 12 patients with central olfactory disorders. The positive EOG rate in patients with peripheral or central olfactory disorders was 28 and 69%, respectively. Moreover, the positive rate was decreased in proportion to the degree of olfactory disorders in chronic rhinosinusitis. No positive EOG was observed in anosmic nasal cavities, while it was 68% in normal persons and resulted in an equal value to anosmia group caused by the damage of central olfactory pathway. From these results, it is considered that the positive EOG study is the only method which provides objective finding for the differential diagnosis of anosmia caused by disorders of olfactory epithelium or disorders of central olfactory tract.

Chronic Disease↗

PubMed1989

Ranula and sarcoid granuloma of a sublingual gland.

A case is reported in which the treatment of the ranula consisted of dissection of the cystic wall along with excision of the sublingual gland of origin. The histological picture of the gland was consistent with sarcoidosis. Thereafter, all other examinations were negative for sarcoidosis. However, there remains the possibility that the lesion may be the precursor of widespread sarcoidosis which might occur in the future.

Adult↗

PubMed1989

Reevaluation of treatment of ranula.

Ranulas or mucoceles of the floor of the mouth are cysts arising from the sublingual glands. Experience with 27 such patients over the past 10 years is reviewed. Successful treatment was achieved by excision of the cyst with the sublingual gland.

Adolescent↗

PubMed1989

Synergistic antitumor activity of cisplatin and UFT in nude mice bearing transplantable nasopharyngeal hybridoma.

Combination therapy with cisplatin (CDDP) and UFT, a drug prepared with 1-(tetrahydro-2-furanyl)-5-fluorouracil and uracil at a mixing molar ratio of 1:4, was examined in nude mice bearing transplantable human nasopharyngeal hybrid cell (A2L/AH). The tumor growth of A2L/AH was inhibited in the group administered UFT 20 mg/kg, but was not in 10 mg/kg group in comparison with the control group. An inhibition rate (IR) of the tumor growth at 20 mg/kg and 10 mg/kg doses was 80.2 and 21.3%, respectively. The group received CDDP (5 mg/kg, q7d x 3, 2 mg/kg, q7d x 3, and 1 mg/kg, qd x 6) by intraperitoneal injection, resulted in 75.2, 37.4, and 23.1% inhibitions, respectively. While, the response rate in the group treated with CDDP (1 mg/kg, qd x 6) and UFT (10 mg/kg) showed a synergistic effects (IR, 66.3%) which was higher than in the group administered CDDP (2 mg/kg, q7d x 3) and UFT (10 mg/kg) (IR, 58.3%).

Administration, Oral↗

PubMed1989

The human nasopharynx as a reservoir for Epstein-Barr virus.

Nineteen human nasopharyngeal tissue specimens biopsied from patients with suspected nasopharyngeal carcinoma (NPC) and 15 adenoid tissue specimens obtained by surgery were successfully grown in culture without bacterial and fungal contamination. Fibroblastic growth developed in all of the cultures. In some cases, epithelial growth was also seen among the fibroblastic growth. The cells became round and floated in the culture fluid between 19th and 57th day of culture in 6 out of 10 NPC tissue cultures, 3 out of 9 tissue cultures of apparently normal nasopharyngeal mucosa of patients referred for exclusion of NPC, and 4 out of 15 adenoid tissue cultures. The floating cells consisted of B-lymphoblastoid cells and were positive Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA). The data suggest that a small number of B-lymphocytes in the human nasopharynx serve as a reservoir for EBV.

Adenoids↗

PubMed1989

Intravagal parathyroid adenoma.

An extremely rare case of intravagal parathyroid adenoma is presented. The tumour caused fusiform swelling of the left vagus nerve was shelled out. Post-operatively the left recurrent nerve palsy was recovered in the two months. Serum calcium level returned to normal on the tenth day after the surgical operation without symptoms of hypocalcaemia.

Adenoma↗

PubMed1989

Agenesis of the cervical internal carotid artery.

Total or segmental agenesis of the internal carotid artery is a rare anomaly. The cervical portion of the internal carotid artery was absent in the right side of the patient who was carried out radical surgery due to recurrent oropharyngeal cancer. Post-operative venous digital subtraction angiography revealed that the remaining intracranial portion of the internal carotid artery was normally patent and supplied blood flow via ipsilateral external carotid artery. Otolaryngologist-Head and Neck surgeon should know such a vascular anomaly and avoid a disastrous result on dividing the external carotid artery.

Carotid Artery, Internal↗

PubMed1989

Three cases of inter-sterno-costo-clavicular ossification associated with tonsillar infection.

Three cases of inter-sterno-costo-clavicular ossification (ISCCO) associated with chronic tonsillitis are reported. Two cases had additional symptoms of pustulosis palmaris et plantaris (PPP). All the patients underwent tonsillectomy. The histological changes of the palatine tonsils of case 1 and case 2 were characterized by so-called focal small lacunar ulcers and periarteriolar fibrosis (onion-skin lesion). Some multinucleated giant cells were observed in case 2. Following tonsillectomy, symptoms in the clavicular region and the PPP improved. It is possible to consider that ISCCO is caused by focal tonsillar infection.

Adult↗

PubMed1989

Pharyngeal cyst arising from second branchial cleft.

A rare case of pharyngeal cyst arising from the second branchial cleft in a 14-year-old boy is described. A cyst located in the right posterolateral wall of the oropharynx was completely removed by an intraoral approach. Histopathological examination revealed that the cyst was lined with columnar (respiratory type) epithelium.

Adolescent↗

PubMed1989

Fibrin glue in the surgical treatment of ranulas.

The essential treatment of a ranula is meticulous dissection of the thin wall of the cyst in continuity with the sublingual gland of origin. We have used a technique of fibrin glue injection into the cystic space of the ranula after it had been evacuated by aspiration. The fibrin glue within the cystic cavity prevents collapse of the wall of the cyst during surgery and facilitates and simplifies the surgical procedure by clearly outlining the involved area and by sharply delineating its thin wall.

Fibrin Tissue Adhesive↗

PubMed1989

Epstein-Barr virus (EBV) infection by cocultivation of fibroblast-dominant cell line and EBV-transformed lymphocytes.

A long-term cell culture epitheloid cell line (NPC-F/L) was established from the fibroblast-dominant cell line derived from nasopharyngeal carcinoma tissue by cocultivation with an Epstein-Barr virus (EBV)-transformed lymphoblastoid cell line of adenoid origin. The cells were investigated by karyotyping, by immunofluorescent testing for EBV-associated nuclear antigen (EBNA), early antigen (EA), and viral capsid antigen (VCA) and by a light microscopy. The cells were shown to be epithelial not only by morphology but also by immunocytochemical staining with monoclonal antibody to cytokeratins and they expressed EBNA and EA but not VCA. The cells developed histologically undifferentiated squamous cell carcinoma when inoculated subcutaneously into the back of athymic nude BALB/c(nu/nu) mice, which were similar to the human tumors from which the fibroblastoid cell line was established. The presence of EBNA in touch smears prepared both from the biopsy tissue of human and nude mice was demonstrated. Karyotyping analysis indicated an aneuploid chromosome with a model number of 74.

Cell Line↗

PubMed1989

Spontaneous lymphoblastoid transformation of cells obtained from human tonsils.

Spontaneous lymphoblastoid transformation of cells obtained from 25 human tonsils were investigated. Three Epstein-Barr virus (EBV) genome-positive lymphoblastoid cell lines were spontaneously established in tissue cultures in vitro from human tonsils. In addition, the frequency of spontaneous lymphoblastoid transformation was increased by the treatment of tumor promoter, 12-0-tetradecanoylphorbol-13-acetate and 18 lymphoblastoid cell lines were established. The data suggest that the human tonsil is a reservoir for EBV.

Adolescent↗

PubMed1989

Characteristics of Epstein-Barr virus-carrying lymphoblastoid cells derived from human tonsil: EBV production and autoantibody response.

Tonsillar and peripheral blood lymphocytes from 20 patients with tonsillitis were examined for production of Epstein-Barr virus (EBV) and antinuclear antibodies within 2 weeks after EBV transformation. All established lymphoblastoid cell lines (LCLs) contained more than 90% EBV nuclear antigen-positive cells. However, no LCLs produced biologically active virus. On the other hand, 10 (50%) out of 20 tonsillar LCLs and 4 (20%) out of 20 peripheral blood LCLs produced autoantibodies. These results showed that there are differences in an EBV-induced lymphocyte autoantibody response between tonsillar lymphocytes and peripheral blood lymphocytes.

Antibodies, Antinuclear↗

PubMed1989

Development of significant sternoclavicular joint hypertrophy following radical neck dissection.

Radical neck dissection (RND) has proved to be effective in the en bloc removal of metastatic lymph nodes in the neck. This paper describes a case with severe sternoclavicular joint hypertrophy following RND. This condition should be differentiated both clinically and radiologically from metastases of the head of the clavicle. Computed tomography was found to be of value in the diagnosis of the condition.

Aged↗

PubMed1989

Malignant fibrous histiocytoma arising from the vocal cord.

A rare case of malignant fibrous histiocytoma of the left vocal cord occurring in an 80-year-old man is described, with a review of the literature. There has been no recurrence for 24 months since the initial endolaryngeal microsurgery.

Aged↗

PubMed1989

Mesenchymal chondrosarcoma of the ethmoid sinus.

Mesenchymal chondrosarcoma arising from primitive mesenchymal cells is a distinctive type of chondrosarcoma that exists in both skeletal and extraskeletal forms. The tumor is composed of undifferentiated cells with focal areas of cartilaginous or chondroid matrix. An extremely rare case of mesenchymal chondrosarcoma of the anterior and middle ethmoid sinuses in a 16-year-old girl is presented. Magnetic resonance imaging provided more anatomic information about the tumor than computerized tomography and aided in surgical planning.

Adolescent↗

PubMed1989

Differences in the ability of cells to fuse are mediated by strains of Epstein-Barr virus.

Epstein-Barr virus (EBV) preparations from both NPC-KT cells (NPC-EBV) and P3HR-1 cells (HR-1-EBV) can induce cell fusion between EBV receptor (EBVR)-positive Raji cells and EBVR-negative cells, but other strains of EBV cannot induce cell fusion. The effect of these two EBV isolates on ability of cells to fuse has been studied to determine if there are differences in the biological properties of the different EBV isolates, particularly the isolates obtained from nasopharyngeal carcinoma such as NPC-EBV. The frequency of cell fusion between NPC-EBV-superinfected Raji cells and EBVR-negative epithelial cells (Ad-AH) was increased more than 30-fold in the presence of medium containing 1% dimethylsulfoxide (DMSO). However, the frequency of cell fusion between HR-1-EBV-superinfected Raji cells and Ad-AH cells was unaffected under the same conditions. The data show that differences in the ability of cells to fuse are induced by variants of EBV in response to DMSO. These differences may be important in elucidating the different biological properties of EBV isolates and might have implications for the pathophysiology of EBV-associated illness.

Cell Fusion↗

PubMed1989

Further studies on growth characteristics of Epstein-Barr virus (EBV) genome-positive and -negative epithelial hybrid cell lines.

Differences in tumorigenicity between the Epstein-Barr virus (EBV) genome-negative and -positive epithelial A2L/AH hybrid cell lines have been described recently. Further differences in growth characteristics of the two epithelial hybrid cells have since been examined. Differences were found in doubling time and cloning efficiency in agarose of the two cell lines. In addition, the EBV genome-negative epithelial hybrid cell line (cl-654), unlike the EBV genome-positive epithelial hybrid cell line (cl-1), failed to grow in the unmanipulated nude mice, but was successfully transplanted in 60Co-irradiated nude mice. The histopathologic findings of tumors induced by the inoculation of cl-654 cells in nude mice showed more differentiated carcinoma than the findings of tumors induced by cl-1 cells. The data suggest that EBV is associated with an alteration of cell differentiation in epithelial cells as well as tumorigenicity in nude mice.

Animals↗