3T3-L1 preadipocytes differentiate into cells having the biochemical properties of adipocytes; tumor necrosis factor-alpha (TNF), retinoic acid (RA), and transforming growth factor-beta (TGF-beta), attenuate this process. Inhibition of differentiation by these agents, thought to be at the level of transcription, has been investigated by examining the accumulation of mRNA for six transcription factors and the autocrine growth factor interleukin 6 (IL-6). Upon induction of differentiation, a rapid and major accumulation of c-fos and jun-B mRNA was observed that returned to near basal levels within 4-6 h. In contrast, c-jun mRNA, although rapidly expressed following induction of differentiation, remained at relatively constant levels throughout the time course. Exposure of the cells to 5 nM TNF potentiated the accumulation of all 3 mRNAs but most significantly c-jun (12-fold), which remained elevated for at least 24 h after treatment. In control differentiating cells, krox-20 and fox-B were expressed transiently, 30 min to 2 h, while fra-1 mRNA accumulated over an extended period, 1 to 8 h. Again, TNF enhanced the accumulation of these mRNAs. Accumulation of mRNA for C/EBP, a transcription factor proposed to control expression of genes involved in the terminally differentiated state was attenuated after exposure of the cells to TNF. C/EBP expression was also inhibited in cells exposed to RA or TGF-beta. IL-6 mRNA was expressed briefly (30 min to 2 h) and again transiently (at 8 h after induction of differentiation). TNF treatment markedly enhanced accumulation of IL-6 message.(ABSTRACT TRUNCATED AT 250 WORDS)

1-Methyl-3-isobutylxanthine↗

PubMedDate not supplied

Community care givers' attitudes toward nursing homes.

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Aged↗

PubMedDate not supplied

Adolescent knowledge and attitudes about abortion.

A focus-group study of adolescents from cities across the United States revealed that they lacked accurate knowledge about abortion and the laws governing it. Most expressed erroneous beliefs about abortion, describing it as medically dangerous, emotionally damaging and widely illegal. The study also revealed that antiabortion views, conservative morality and religious beliefs were the primary sources of these adolescents' attitudes toward abortion. In general, the participants in the study said they were personally opposed to abortion, but supported its continued legality as a woman's choice. Although most of the teenagers expressed positive feelings toward parents, they did not feel that mandatory parental involvement would be helpful, and in some cases could cause harm.

Abortion, Legal↗

PubMedDate not supplied

A nursing concern.

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Humans↗

PubMedDate not supplied

The significance of fibronectin in cryoprecipitation in rheumatoid arthritis and other diseases.

Fibronectin, a glycoprotein produced by mesenchymal cells, was present in 11 of 16 plasma cryoprecipitates and 12 of 14 synovial fluid (SF) cryoprecipitates. In some SF cryoprecipitates it was the major protein component. Fibronectin levels were related to the development of serum turbidity in the cold and fibronectin was involved in the development of cold turbidity induced by some charged polysaccharides in plasma, serum, and SF. It is suggested that fibronectin, which is synthesized by vascular endothelial cells and synovial lining cells, is involved in the development of some cryoprecipitates in patients with rheumatoid arthritis and other diseases.

Arthritis, Rheumatoid↗

PubMedDate not supplied

[Rising temperatures and health risks].

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Hot Temperature↗

PubMedDate not supplied

Acute abdominal pain.

Abdominal pain is among the most frequent ailments reported in the office setting and can account for up to 40% of ailments in the ambulatory practice. Also, it is in the top three symptoms of patients presenting to emergency departments (ED) and accounts for 5-10% of all ED primary presenting ailments. There are several common sources for acute abdominal pain and many for subacute and chronic abdominal pain. This article explores the history-taking, initial evaluation, and examination of the patient presenting with acute abdominal pain. The goal of this article is to help differentiate one source of pain from another. Discussion of acute cholecystitis, pancreatitis, appendicitis, ectopic pregnancy, diverticulitis, gastritis, and gastroenteritis are undertaken. Additionally, there is discussion of common laboratory studies, diagnostic studies, and treatment of the patient with the above entities.

Abdomen, Acute↗