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Biomedical subjects

R Sommer

Publications and source records attributed to R Sommer.

115 records · Page 7Linked to original sources
PubMedDate not supplied

Computer support of DNA sequence analysis.

The paper describes a package of APL-programs suited for the management and the analysis of DNA sequence data. Most of the application programs are related to experimental work in a DNA sequencing laboratory: Search for overlapping DNA fragments to construct complete DNA sequences; search for restriction sites; computing fragment patterns when cutting a DNA with restriction enzymes; plotting physical and genetic maps of a DNA; search for homologies as well as various counting procedures. More sophisticated programs are concerned with the prediction of RNA secondary structure and its graphical representation.

Base Sequence↗
PubMedDate not supplied

Measurement of UV radiation using suspensions of microorganisms.

The measurement of solar UV radiation is usually performed using physical devices like photodiodes or photomultipliers or with chemical substances (actinometry). The application of biological material such as microorganisms for this purpose has gained increasing importance in the last few years. The microorganisms may be dried and spread on a flat surface or they may be in aqueous suspensions contained in UV-transparent vessels. If the measurements are done on flat surfaces, the irradiance weighted by the action spectrum of the dried microorganism used is the result of the measurement. If aqueous suspensions of microorganisms are used, contained for instance in spherical vessels, the fluence weighted by the action spectrum of the microorganisms in the aqueous suspension is the result. A problem of this method of measurement can be that inside the vessel the distribution of UV radiation is usually not homogeneous, causing distributions of fluences among the irradiated microorganisms, which may result in variation of the results depending on the mixing characteristics of the suspension during irradiation.

Bacillus subtilis↗
PubMedDate not supplied

[Gentamicin dosage in newborn infants].

Sixty nine pairs of trough and peak serum levels of gentamicin were determined in sixty three cases of gentamicin therapy of suspected sepsis in newborn babies (dosage 3.46 +/- 0.42 mg/kg/day given once a day). Eighty four percent of the trough levels were below 2 mg/l (mean: 1.39-0.60 mg/l). Ninety three percent of the peak levels were between 4 mg/l and 12 mg/l (mean: 7.38-2.35 mg/l). Three peak levels (four percent) were in the potentially toxic range (above 12 mg/l) and eleven trough levels (sixteen percent) were in the potentially toxic range (above 2 mg/l). On the basis of these observations the recommended gentamicin doses during the first two weeks of life is 3-4 mg/kg/day applied once a day. In immature babies with birth weights below 1500 g the daily doses should not exceed 3 mg/kg were as term babies especially during the second week of life should be treated with a daily gentamicin doses of near to 4 mg/kg/day.

Ampicillin↗
PubMedDate not supplied

[Early diagnosis and early treatment of hypophosphatemic vitamin D-resistant rickets].

This is a report on two children with hypophosphatemic vitamin D-resistant rickets born to affected mothers diagnosed at the age of one and three months, respectively. The most important diagnostic criterium is the reduced value of the transport maximum of phosphorus in relation to glomerular filtration rate. At the age of one and six months, respectively, therapy with 1.25 (OH)2D3 and phosphate was started with the result of normal growth of length, radiological healing of rickets and normalisation of alkaline serum-phosphatase, but persistently low fasting values of serum-phosphorus during the time of observation up to twelve and twenty-four months of age, respectively in both children. The therapy with 1.25 (OH)2D3 and phosphate has a positive effect on intestinal absorption of phosphorus, because the serum-phosphorus is significantly elevated to the age corrected normal range two hours after oral administration of phosphate. There were no negative effects during therapy. It seems, therefore, that early diagnosis and therapy with 1.25 (OH)2D3 and phosphate is successful in healing this rare form of rickets.

Calcitriol↗
PubMedDate not supplied

[Acquired immunologic deficiency syndrome (AIDS) in an infant from Austria].

Manuel born in June 1984 is presented for the first time at the age of 6 months with the following symptoms: Otitis purulenta, enlargement of lymph-nodes, swelling of liver and spleen, enteritis, failure to thrive and candida albicansdermatitis. Immunoglobulins and the total serumprotein are elevated. At the age of 9 months a new Hospital admission was necessary. The following symptoms are present: severely enlarged lymph-nodes at different sites, enlargement of liver and spleen, interstitial pneumonia and enteritis. Body weight is below the third percentile. Tine-Test negative. The baby was BCG vaccinated after birth and at 3 months the Tine-Test was positive. The serologic antibody tests (Elisa and Western Blot) for HTLV III are positive, the index helper: suppressor T cells is 1,1 (normal above 1,5). The antibody titers in the maternal blood are higher than in the child. The mother was a regular drug user. A vertical perinatal transmission from the affected mother to her baby is postulated. The start of clinical illness was the 5th month of life. AIDS was fully developed at 9 months of age. Cotrimoxazol treatment resulted in clinical improvement.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

[Transitory pseudohypoparathyroidism in the newborn infant].

This is a report on two young infants suffering from transitory pseudohypoparathyroidism. Therapy with vitamin D in high doses was not helpful but metabolic changes were promptly corrected by the administration of 1.25-(OH)2-D3 (Rocaltrol). The typical pretreatment findings were: hypocalcemia, hyperphosphatemia, reduced clearance of phosphate. Parathormone in serum was normal. Treatment with 0.25 and 0.5 micro-Gram 1.25-(OH)2-D3 respectively resulted in prompt correction of serum calcium- and serum-phosphate-changes. The observation of the urinary-cAMP-excretion during the course of the disease enables one to differentiate between the permanent and the transient form of the disease: values reaching 5-10 nmol/mg Creatinine indicate the time when treatment can be discontinued in the transitory form.

Calcitriol↗