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Biomedical subjects

R Sanz

Publications and source records attributed to R Sanz.

63 records · Page 4Linked to original sources
PubMedDate not supplied

Determination of viable yeast cells by gravitational field-flow fractionation with fluorescence detection.

Vinification processing is largely related to yeast performance and depends on the initial cell viability. To optimize the quality of wine fermentation, control of the yeast quality is mandatory. The present paper describes a new method using gravitational field flow fractionation (GrFFF) with fluorescence detection for the determination of yeast cell viability before the fermentation process. A GrFFF calibration procedure was developed using commercial yeast to prepare standards of viable cells and propidium iodide (PI) as fluorescent probe for nonviable cells. The suitability of the new method was tested with several commercial yeast strains with a g/L content ranging from 1 to 3. The validation of the method was performed by comparing GrFFF viability values with those obtained using Coulter counter and flow cytometry techniques.

Cell Separation↗
PubMedDate not supplied

Use of fluorescent probes for determination of yeast cell viability by gravitational field-flow fractionation.

The quality of wine greatly depends on the features of the yeast used in its production, and yeast cell viability is one of the most important quality control issues to consider in this regard. In the first steps of winemaking, the use of a low-cost and simple methodology for monitoring the cell viability of yeast inoculates is of paramount importance. Gravitational field-flow fractionation is a useful technique for the determination of cell viability because it provides gentle experimental conditions, although the proper use of fluorophore probes as biomass indicators is required. In this paper the use of different fluorescent probes such as carboxyfluorescein diacetate (cFDA), calcein-AM, and SYTO-13 were considered as viability biomarkers. Calceina-AM allowed the establishment of a direct GrFFF method to determine cell viability, with a limit of detection of 5.0 x 10(4) viable cell/mL. SYTO-13 could be used as biomass indicator with a limit of detection of 3.5 x 10(4) total cells/mL. The suitability of the procedure was tested with three commercial yeast samples, and the results were compared with those obtained using standard techniques.

Calibration↗
PubMedDate not supplied

Treatment of multiple viral warts with levamisole.

Seventy-seven patients with multiple common warts were screened for a double-blind study using levamisole. Of these, only 32 patients showed cellular immune deficiency. Fourteen patients received 2.5 mg/kg, two days a week, for eight weeks, and eighteen received a placebo. Both groups showed some improvement but results were not statistically different. Placebo plays a significant role in the treatment of warts.

Clinical Trials as Topic↗
PubMedDate not supplied

Sonographic, cytogenetic and DNA analysis in four 69,XXX fetuses diagnosed in the second trimester.

OBJECTIVE: To describe the ultrasound findings and its relationship with the cytogenetic study and the origin of the extra haploid chromosome set in four 69,XXX cases. METHODS: Four pregnant women were referred because of abnormal 2nd trimester ultrasound. Karytoypes, FISH and DNA analysis were performed. RESULTS: All cases presented asymmetrical intrauterine growth retardation, marked oligohydramnios and placental alterations and showed a 69,XXX karyotype. In three cases, DNA analysis allowed to establish the origin of the extra haploid chromosome set. CONCLUSIONS: At least three fetuses had a maternal extra haploid chromosome set. Thus, it has been possible to establish the main ultrasonographic markers and to observe the survival of the fetus until the second trimester when they have a maternal origin.

Adult↗
PubMedDate not supplied

Chromosomal mosaicism for isochromosome 11q confined to CVS direct preparations.

OBJECTIVE: To analyse the discrepancy between the karyotype in direct preparations of chorionic villus sampling (CVS) and the fetal karyotype and its possible fetal phenotypic repercussion. METHODS: The karyotype was obtained from direct and cultured preparations of CVS. FISH was performed in direct CVS preparations and in four different areas of term placenta. RESULTS: Karyotype and FISH analysis in CVS revealed a 46,XX/47,XX,+i(11q) cell line. Cultured CVS preparations showed a 46,XX karyotype. Cytogenetic studies in term placenta did not reveal the abnormal cell line. Molecular studies did not detect uniparental disomy for chromosome 11 in the fetus. CONCLUSION: The fetus, at birth, had no phenotypic abnormalities. IUGR was not present during gestation, in accordance with the low proportion of aneuploid cells in term placenta, and UPD for chromosome 11 was not observed.

Adult↗
PubMedDate not supplied

[Normal values for basal serum gastrin with and without stimulation].

In the present work we report a study of the basal gastrin serum levels and stimulated serum gastrin levels after an hyperproteic meal, in a group of 20 healthy volunteers, with neither actual nor previous digestive symptoms. 60% were males, with a mean age of 35, 7 years, the highest percentage (40%) belonging to the fourth decade of life. The procedure used to determine serum gastrin levels is described. The mean value of the 3 samples obtained in basal conditions was of 51.01 pg/ml. The mean value of the 6 samples obtained after the stimulation meal was of 73.4 pg/ml. The results obtained in this study are discussed and compared with the ones of the reviewed literature.

Adolescent↗
PubMedDate not supplied

[Hormonal markers of stress in acute cerebrovascular pathology].

Various studies carried out over the last decade have shown that high glucose levels in the blood foster ischaemic brain damage associated with a worse evolution of such pathologies. The aim of the study we performed was to try to shed some light on whether stress in these patients raised their glucose levels adding to a worsening of the patient's clinical picture. We studied 318 consecutive patients suffering from stroke. We determined fasting glucose levels, prolactin and cortisol within the first few hours of hospitalization and afterwards at seven to ten days and again at one month after the stroke. Clinical severity was evaluated using Toronto and Mathew neurological scales and the degree of incapacity was measured using the Barthel functional scale on the three aforementioned occasions and Rankin's modified scale six and twelve months after the stroke. Clinical severity the first hours after stroke was significantly related to glucose levels, such relationship not being observed with prolactin and cortisol. Nor did we observe any significant association between glucose and these hormones. Likewise the anxiety scale had no relationship with any hormone. Studying medium and long term functional incapacity, glucose significantly correlated with the Rankin scale although with low dependence, such a relationship not being found either with prolactin or cortisol. Our work would seem to indicate that blood glucose behaviour is independent of prolactin and cortisol levels since we found no such relationship between them.

Aged↗