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Biomedical subjects

R Sakazaki

Publications and source records attributed to R Sakazaki.

At least 73 records · Page 4Linked to original sources
PubMed1982

Serological comparison of two collections of Vibrio cholerae non O1.

Two large, independently obtained collections of vibrio cholerae non O1, containing 59 and 67 reference strains, respectively, were compared serologically in four laboratories. Twenty strains in each collection were considered identical, and an additional 16 strains in each collection were probably identical. Twenty-eight unique strains were identified, and inconclusive results were obtained with 25 strains. Nine strains were not considered V. cholerae in at least one testing laboratory. Of these, five showed insufficient correspondence to V. cholerae O1 by DNA hybridization to be considered V. cholerae.

Agglutination Tests↗
PubMed1981

A new Salmonella serovar: Salmonella itami (9,12:1,z13:1,2).

A new Salmonella serovar, here names Salmonella itami, was isolated from a traveller suffering from gastroenteritis, who had just returned from Thailand. The isolate belonged to the subgenus I of Salmonella, and the antigenic formula was determined to be 9,12:1,z13:1,2.

Humans↗
PubMed1981

Vero cytotoxin produced by Escherichia coli strains of animal origin.

A heat-labile Vero cytotoxin (VT) was detected by the Vero cell assay in the culture supernatants of ten Escherichia coli strains isolated from a variety of clinical sources. The VT titer varied with strains, ranging from 1:8 to 1:2,048. No VT was found to be cytotoxic for Y1 mouse adrenal cells or to induce fluid accumulation in the rabbit or porcine ileal loops. No strains produced a heat-labile enterotoxin as defined by either tissue culture or the standard ileal loop reaction. Three strains originated from piglets with diarrhea were confirmed by the infant mouse reaction to produce a heat-stable enterotoxin. Neutralization studies revealed antigenically the presence of two different forms of VT produced by E. coli strains of animal origin.

Animals↗
PubMed1980

A survey of Plesiomonas shigelloides from aquatic environments, domestic animals, pets and humans.

We conducted a survey during the period from 1974 to 1976, to determine the distribution of Plesiomonas shigelloides in human faeces, the intestinal contents of cattle, swine, poultry, dogs, cats, fresh water fish, and river water and sludge from wet riverbeds in the vicinity of Tokyo. Isolation of the organisms was performed by using Salmonella-Shigella (SS) agar and deoxycholate-hydrogensulphide-lactose (DHL) agar plates. P. shigelloides was isolated from 3 (0.0078%) of 38454 healthy Tokyoites, 37 (3.8%) of 967 dogs, 40 (10.3%) of 389 cats, 25 (10.2%) of 246 fresh water fish, 64 (12;8%) of 497 river water samples, and 2 of 19 (10.5%) sludge samples. Of 302 strains isolated, from dogs, cats, fresh water fish, river water and healthy carriers, 196 were typed to 50 serovars. Most of the serovars were found to be similar to strains isolated from patients with gastroenteritis due to P. shigelloides.

Animals↗
PubMed1980

Isolation of octapeptin D (studies on antibiotics from the genus Bacillus. XXVII).

A new peptide antibiotic complex, named octapeptin D, was isolated from culture broth of a microorganism belonging to the genus Bacillus. The trihydrochloride of the antibiotic was obtained as a colorless powder, soluble in water and methanol. The empirical formula, C47H88N12O11.3HCl.H2O, was indicated by elemental analysis. Amino acid analysis on the acid hydrolyzate demonstrated the presence of 2,4-diaminobutyric acid (4 moles), serine (1 mole) and leucine (3 moles). Gas chromatographic analysis with the methylated product of the ethereal extract of the acid hydrolyzate revealed the presence of beta-hydroxy isodecanoic acid, beta-hydroxy decanoic acid, beta-hydroxy isoundecanoic acid and beta-hydroxyanteisoundecanoic acid. Octapeptin D is active against Gram-negative and Gram-positive bacteria in vitro and in vivo.

Animals↗
PubMed1979

The structures of tridecaptins B and C (studies on antibiotics from the genus Bacillus. XXV).

On examining the structures of the antibiotics tridecaptins B and C, the constituent amino acids were separated and their chiralities were determined. The constituent fatty acids were identified by gas chromatography and mass spectrometry. Cleavage with N-bromosuccinimide and sequential analysis by EDMAN degradation demonstrates the sequence of the C-terminal side of tridecaptins B and C. Deacylation with polymyxin acylase of tridecaptin B and successive EDMAN degradation revealed the sequence of the N-terminal side of tridecaptin B. Finally partial acid hydrolysis on tridecaptin C clarified the sequence of the N-terminal side of tridecaptin C.

Amino Acids↗
PubMed1979

Numerical classification of Yersinia enterocolitica and relationship between its subdivision and pathogenicity.

A total of 102 strains of Yersinia enterocolitica were studied on their morphology, physiology and biochemistry at 25 degrees C. All data coded in binary notation and similarities between each pair of strains by simple matching coefficient were calculated using subsequent computation. Four clusters were distinguished within the 102 strains at a similarity level of 90--93%. Although some discrepancies were recognized on reactions of indole, xylose, esculin and salicin, it was evident that 3 of the 4 groups in the present study correspond to the DNA relatedness groups of Brenner et al., respectively. On the other hand, it was confirmed, using feeding tests with monkeys, that only the group 1 was enteropathogenic. These results support the suggestion of Brenner et al. that their DNA relatedness group 1 is true Y. enterocolitica.

Animals↗