The gastrointestinal absorption of the beta blocker oxprenolol was investigated in four healthy subjects by an intubation technique. Oxprenolol was introduced into the stomach, dissolved in a homogenized meal containing the marker 14C-polyethylene glycol (PEG) 4000. Unlabeled PEG 4000 was perfused during the whole experiment into the duodenum at the ampulla of Vater. Samples of luminal contents were collected at regular intervals over four hours in the stomach, at the angle of Treitz, and 30 cm below this point. Blood was also collected. Oxprenolol was not absorbed in the stomach. About 80% of the drug emptied from the stomach was absorbed in the duodenum, and 80% of that released from the duodenum was absorbed in a 30-cm segment of the jejunum. The amounts absorbed in these two intestinal segments were directly proportional to the amounts delivered. The areas under the plasma concentration-time curves were not related to the amounts absorbed. A single dose of oxprenolol taken with an homogenized meal did not modify the gastric emptying and secretory response.

Absorption↗

PubMedDate not supplied

[Circadian aspects of iron metabolism. Changes of total iron binding capacity in patients with liver cirrhosis and in apparently healthy persons].

A group of 8 patients (M) with liver cirrhosis were studied. Each subject was standardized with rest time extending from 22,00 to 05,00 and meals scheduled at 08,00-08,30, 12,00-12,30, 17,30-18,00. Then was sampled at 04,00, 08,00 12,00, 16,00, 20,00, 24,00. In each sample of serum were determined iron, T.I.B.C. and L.I.B.C. All data were analyzed by macroscopic approach (chronograms) and by mean cosinor. A significant circadian rhythm was detected in all parameters, with a statistically valid difference between normal subjects and patients with liver cirrhosis in the mesor of T.I.B.C. and L.I.B.C.

Adolescent↗

PubMedDate not supplied

Circadian rhythms in blood, urine and gastric acid secretion of presumably healthy volunteers.

The mean conisor technique reveals statistically significant circadian rhythms for serum concentrations of iron, chloride, cortisol, PBI, the fibrinolytic activity of blood, urinary iron excretion and gastric acid secretion in studies covering a 24-h span for presumably healthy subjects. Quantification of these rhythms provides internationally comparable reference values both for the conventional clinical laboratory and for any special chronobiologic applications.

Adolescent↗

PubMedDate not supplied

[Post-surgical results of 48 septal perforations].

We have studied the post-surgical results of 48 perforations of the nasal septum, regarding their etiology, location and size, which must be taken into account when deciding which closure technique to use.

Adult↗

PubMedDate not supplied

[Exerimental study of enterotoxic activity in some strains of Salmonella typhimurium: observations on the model of ADP-induced platelet aggregation].

In a previous report it has been shown that enterotoxin preparations (cholera and E. Coli) able to activate adenylatecyclase system, abrogated platelet aggregation induced by ADP. In the present study the same experimental model has been applied to some purified filtrates from Salmonella typhimurium strains. Preparations from 986 and TLM (invasive strains causing also fluid secretion) interfere with platelet aggregation, while 1027 strain (invasive but not evoking fluid secretion) didn't show such effect. It has been argued, as Sandefur and Peterson have demonstrated with different experimental models, that the system of platelet aggregation induced by ADP is valid for some strains of Salmonella typhimurium, which act with a mechanism partially involving adenylatecyclase system (i.g. cholera-like).

Adenosine Diphosphate↗

PubMedDate not supplied

[Experimental contribution to the study of enterotoxigenic activity of some "Salmonella typhimurium" strains: investigations with the model of the platelet aggregation by ADP (author's transl)].

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Adenosine Diphosphate↗

PubMedDate not supplied

[Circadian changes in serum prolactin in a group of apparently healthy subjects].

Serum immunoreactive prolactin was evaluated in 16 female and 5 male apparently healthy subjects aged 21-76 yr. After a period of synchronisation, with sleep from 22 to 5 and standardized meals at 8-8.30, 12-12.30, 17.30-18, venous blood samples were taken at 4, 8, 12, 16, 20 and 24. The radioimmunological data were processed using an ordinary chronogram technique and quantified by mean cosinor method. A significant (p less than 0.05) circadian rhythm was noted, with an acrophase at 1.45, amplitude of 2.17 and Mesor 6.89. Chronobiological studies are thus needed to determine the best normal temporal values of prolactin when setting control standards in pathological situations.

Adult↗

PubMedDate not supplied

[Bacteriocins as cytotoxic agents in experimental neoplasms: considerations on the role of possible contaminants].

In most recent papers some Authors have demonstrated that several bacteriocins are able to markedly inhibit the division of various neoplastic mammalian cell lines; also in vivo the inhibition of tumor growth or even regression relative to controls are statistically significant. Since such preparations are "crude" and it's possible that contaminating agents may represent a possible source of variability in the results, mostly in vivo; we have examined a preparation of Pyocin employed in this study for the possible endotoxin contamination using the Limulus assay. Such test was positive in triplicate and semiquantitative determination has shown and endotoxin amount of 5 to 10 microgram/ml. This level is, in vitro, not anough to give cytotoxic effect, but in vivo is able to influence experimental results. In addition, since some bacteriocins and enterotoxins are produced under exactly identical conditions, there is this a possibility that the enterotoxin are coproduced in these preparations and are causing some of the cytotoxic effects on experimental tumors. A more complete separation and purification of preparations will be required before definitive comments.

Animals↗