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Biomedical subjects

R Ono

Publications and source records attributed to R Ono.

At least 55 records · Page 3Linked to original sources
PubMed1989

[Combined treatment of endoscopic laser irradiation and radiotherapy in lung cancer].

Sixty-six patients with lung cancer were treated with endoscopic laser irradiation. In this study, photodynamic laser therapy (PDT) was performed on 28 patients with early lung cancer and 38 patients with advance lung cancer having 69 biopsy-proven malignant lesions of the trachea and bronchus. The patients were administered HpD (2. 5 mg/kg of their weight) injected intravenously three days prior to HpD-mediated laser phototherapy. These patients have completed at least one course of this therapy which was carried out by flexible bronchofiberscope. The laser beam was delivered through an optical fiber connected to the output of a dye laser (630 +/- 3 nm). The optical fiber was passed through the large inside channel of the flexible bronchofiberscope. Among the 69 malignant lesions of the trachea and bronchus, complete responses were obtained in 9 malignant lesions, partial responses in 44 cases, regressions in 13 cases, and the remaining 3 cases had progressions. Of the 66 patients, 19 patients are alive with no recurrence or metastasis, while the remaining 47 patients died after photodynamic laser therapy. The patients treated with photodynamic laser therapy (PDT) had unresectable lung cancer which was observable by bronchofiberscopy but not suitable for radical treatment either by chemotherapy or radiation therapy. We studied a selection of patients with unresectable lung cancer for application of a treatment combining endoscopic laser irradiation and radiotherapy.

Aged↗
PubMed1988

A solid-phase immunoassay to screen anti-HLA monoclonal antibodies.

A solid-phase assay to detect anti-HLA monoclonal antibodies was developed. In this assay microtiter plates are coated with antigens solubilized from cultured lymphoid cells by sonication and then incubated with anti-HLA monoclonal antibodies. The antigen-antibody interaction is indicated by the development of color following the addition of peroxidase-conjugated anti-mouse Ig xenoantibodies and its substrate. The assay is rapid since it does not require centrifugations during the washing steps. Furthermore the assay is simple, reproducible and suitable to screen large numbers of samples and to detect antibodies recognizing determinants not exposed on the membrane of viable cells. The sensitivity of the assay is influenced by the pH of the buffer used to coat plates with antigens, by the number of cells used to prepare soluble antigens, by the incubation time of antigen preparations with plates and by the incubation time of antibody preparations with antigen-coated plates. Titration of anti-HLA monoclonal antibodies with known specificity and screening of hybridomas generated with splenocytes from mice immunized with cultured human lymphoid cells indicate that the sensitivity of the solid-phase assay is similar to that of the ELISA with lymphoid cells.

Antibodies, Monoclonal↗
PubMed1988

Luteinizing hormone-releasing hormone (LHRH) neurons in the male and female rats at peripubertal period.

Luteinizing hormone-releasing hormone (LHRH) neurons were immunohistochemically studied in rats of both sexes at peripubertal ages. The number of immunoreactive LHRH neurons (irLHRH neurons) was counted in the brain region from the level of the septum-preoptic area to the level of the rostral part of the infundibulum in colchicine-treated male and female rats at 30 and 60 days of age. At 30 days, irLHRH neurons were more numerous in male rats than females. At 60 days, the number of irLHRH neurons in female rats increased to the level of male rats of the same age. In non-colchicine-treated rats, the count of irLHRH neuron was quite low. The difference in the number of irLHRH neurons between colchicine-treated and non-treated rats may be regarded as the activity of LHRH system. The difference in the number of irLHRH neurons was larger in male rats than in female rats at 30 days of age. On the contrary, at 60 days of age, the difference was larger in females than in males. LHRH contents were measured in the preoptic-anterior hypothalamic area (POA-AH), where LHRH neuronal perikarya are mainly located, and in the mid-hypothalamic area. LHRH content of the POA-AH in male rats at 60 days of age was not significantly different from that at 30 days of age. While, LHRH content in the POA-AH was greater in 60-day-old female rats at proestrous morning than that in 30-day-old females. At 30 days of age, male rats tended to contain more LHRH in the POA-AH than female rats.(ABSTRACT TRUNCATED AT 250 WORDS)

Aging↗
PubMed1988

Anti-HLA-B7,B27,Bw42,Bw54,Bw55,Bw56, Bw67,Bw73 monoclonal antibodies: specificity, idiotypes, and application for a double determinant immunoassay.

The monoclonal antibodies (MoAbs) KS3 and KS4 are secreted by hybridomas constructed with splenocytes from a BALB/c mouse sequentially immunized with the cultured lymphoid cells JKu and LG-2 which share only the HLA-B27 specificity. Serologic and immunochemical assays have shown that the two MoAbs recognize the same (or spatially close) determinant expressed by HLA-B7,B27,Bw42,Bw54,Bw55,Bw56,Bw67, and Bw73 alloantigens. This determinant is spatially close but distinct from those defined by the anti HLA-B27 monoclonal antibodies described in the literature. The syngeneic antiidiotypic MoAb T12-105 and T12-211 elicited with MoAb KS4 were shown to recognize idiotopes within the antigen combining site of MoAb KS3 and KS4. Neither idiotope was detected on the anti HLA class I and anti HLA class II monoclonal antibodies tested. The MoAb KS4 in combination with the anti human beta 2-microglobulin MoAb NAMB-1 was utilized to develop a double determinant immunoassay (DDIA). The latter represents a sensitive method to detect and quantitate HLA-B27 antigens in spent culture medium of lymphoid cell lines and in serum. Typing for HLA-B27 antigens with the DDIA of sera from HLA typed donors yielded results highly correlated with those of the conventional lymphocytotoxicity assay.

Antibodies, Monoclonal↗
PubMed1988

[Bronchoscopic diagnosis of lung cancer in early stage].

Early lung cancer has been classified into peripheral and hilar types. The peripheral type early lung cancer is the tumor size of under 2 cm with N0, M0, and many cases are detected with Chest X-ray findings. The bronchofiberscopic curettage is performed for its definitive diagnosis. The hilar type early lung cancer is limited within the bronchial wall, and its X-ray findings are normal in most cases. Usually the detection is made with bloody sputum or sputum cytology, and its definitive diagnosis is obtained with the bronchofiberscopic biopsy under direct visualization. In the case with no irregular endoscopic findings in spite of the positive result in sputum cytology its localization can be obtained with special methods such as selective bronchoalveolar lavage, image processing or laser irradiation.

Bronchoscopy↗
PubMed1988

[Clinical studies on S 6472 granule preparation (sustained-release cefaclor) in chronic respiratory tract infections].

S 6472 granule preparation (sustained-release cefaclor) was orally administered to 15 patients with chronic respiratory tract infections (2 acutely exacerbated cases of chronic bronchitis, 13 cases of secondary infections consisting of 1 case of bronchial asthma, 2 cases of bronchial asthma/pulmonary emphysema, and 10 cases of bronchiectasis) at a daily dose of 750 mg divided into 2 doses administered after breakfast and dinner, for a duration of 14 days. The drug was ineffective in 3 of the 10 cases of bronchiectasis but was effective in the other 12 cases, with a rate of efficacy of 80%. There were no side effects of abnormal laboratory findings due to administration of this drug.

Administration, Oral↗