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Biomedical subjects

R Meyer

Publications and source records attributed to R Meyer.

At least 253 records · Page 14Linked to original sources
PubMed1989

[Morphologic findings in dilated cardiomyopathy].

For the morphological characterization of the architecture of the myocardium in dilatative cardiomyopathy hearts of deceased persons as well as after heart transplantation (explanted hearts) were investigated morphologically and histomorphometrically. Thereby could be stated that the cardiomegaly which accompanies this picture of the disease is caused by hypertrophy, increase of the number of collagenous fibres and dilatation of the ventricles. The morphometric investigations made evident that not all cells of the myocardium in the same way participate in the hypertrophy. As a result of the qualitative and quantitative morphological investigations could be stated that in the dilatative cardiomyopathy a particular form of the cardiac insufficiency is concerned which is characterized by an irreversible change of the architecture of the myocardium.

Cardiac Volume↗
PubMed1989

[Differential assessment of myocardial diseases: hemodynamic-angiocardiography studies and etiologic classification].

Subject of our study are 293 patients with diseases of the myocardium, 212 males and 81 females at the average age of 45.2 years who were examined invasively including biopsy of the endomyocardium on account of clinical symptoms and changes of the ECG. Patients with hypertension, vitia, coronary stenoses as well as with hypertrophic cardiomyopathy were excluded. The aims of our study were a precised assessment of the degree of severity by a score from invasive parameters, the characterization of so-called latent or beginning cardiomyopathies and their relations to the dilatative cardiomyopathy as well as the aetiological classification by the biopsy of the endomyocardium. The score resulted from the number and the degree of individual pathological parameters, in which case the degree of the deviation was confirmed with points between 0 and 3. By summarizing of the score three degrees of severity were defined, to which clinical, electrocardiographic and bioptic results were coordinated. In the degree of severity 1 were 170 patients, in the degree of severity 2 42 patients, in the degree of severity 3 81 patients. In the higher degree of severity dyspnoea was significantly more frequently stated. In all degrees of severity the decrease of function was nearly of the same frequency. Of the degree of severity 3 died 28 patients, of the degree of severity 2 1 patient. In the degree of severity 1 by the biopsy of the endomyocardium in greater than 80% aetiological clues were detected, only in 35% in the degree of severity 3. Inflammatory changes were relatively frequently observed in all degrees of severity.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1989

[Ultrasound sonographic diagnosis of myocarditis using quantitative texture analysis of 2-dimensional echocardiography images].

In a prospective study we tested the possibility to differentiate myocardial tissue by means of the quantitative texture analysis of two-dimensional echocardiographic images. 12 patients with a bioptically ascertained myocarditis and 12 healthy patients of a control group were echocardiographically examined. The results demonstrated that the echocardiographic image texture in myocarditis is characterized by an increase of the texture parameters entropy, second difference moment and long-run emphasis as well as by a decrease of the parameters angular second moment, inverse difference moment and run-length nonuniformity as well as by different values of the ring and sector sums of the power spectre. The digital image processing of echocardiography images seems thus to open a new way to the noninvasive diagnostic of myocarditis.

Biopsy↗
PubMed1989

[Ventricular late potentials in patients with cardiomyopathies in relation to electrophysiologic and myocardial biopsy findings].

With the noninvasive registration of late ventricular potentials there is the possibility to obtain statements about a regionally delayed excitation of ventricular parts which may refer to an increased inclination to ventricular tachyarrhythmias. Late ventricular potentials in patients with suspicion of primary diseases of the myocardium more frequently appeared both in the case of inducible non-sustained or sustained ventricular tachycardias and in the presence of simultaneous fibrous hypertrophy of the myocardium and interstitial fibrosis in the bioptate of the myocardium than in noninducibility of ventricular tachycardias and in the absence of histological changes of the myocardium. Thus in patients with cardiomyopathies a certain predicting significance seems to be ascribed to the evidence of late potentials in the highly increased signal-averaged ECG both for the ability to evoke ventricular tachycardias by programmed ventricular stimulation and for the presence of more distinct histological changes of the myocardium.

Adult↗
PubMed1989

Lingual osteoma.

The 29th patient with a lingual osteoma is presented. This is the first case, to our knowledge, with preoperative radiographic and computed tomographic documentation. This rare, benign tumor almost always occurs as a pedunculated solitary mass that arises from the posterior tongue near the foramen cecum. The patients are usually young women who complain wf dysphagia or fullness in the base of the tongue. The imaging findings and theories of the origin of this tumor are described.

Adult↗
PubMed1989

Immature rat epididymal epithelial cells grown in static primary monolayer culture on permeable supports. I. Vectorial secretion.

A cell culture system is characterized for monolayers of immature rat epididymal epithelial cells grown on permeable supports. Cover of the filters was achieved by days 4-5 and was maintained for 9-12 days. The secretion of acid phosphatase (ACP), alkaline phosphatase (AKP) and N-acetylglucosaminidase (NAG) into apical and basal compartments of culture chambers was monitored with time in culture for cells from the proximal and distal epididymis of 37-day-old animals. There was independent secretion of the three enzymes: secretion of NAG and AKP was mainly apical, that of ACP basal; daily secretion of ACP and AKP was constant throughout culture, that of NAG declined; there was greater secretion of NAG and AKP by cells from the proximal than the distal region. The initial high apical secretion of NAG is thought to reflect loss of enzyme from unattached cells, whereas the later AKP secretion is truly directional. Secretion was not influenced by the enzymes used in cell preparation. The cytotoxic agent Thimerosal inhibited secretion of all enzymes when placed beneath the cultures, indicating that secretion depended on viable cells, but initially stimulated release of AKP when applied above the cells possibly reflecting release from the cell membrane.

Acetylglucosaminidase↗
PubMed1989

Immature rat epididymal epithelial cells grown in static primary monolayer culture on permeable supports. II. Histochemistry and ultrastructure.

N-acetylglucosaminidase (NAG), acid phosphatase (ACP) and alkaline phosphatase (AKP) were localised histochemically in fixed cells from the 37-day-old rat epididymis grown in static monolayer culture for 2-8 days. ACP and NAG were cytosolic enzymes found in perinuclear positions, whereas staining of AKP ws consistent with a membranous position. These enzymes were also examined in frozen tissue sections of the epididymis, from rats of the equivalent age, where NAG had intense activity in both supra- and infra-nuclear cytoplasm and ACP was more active apically. For the first time AKP was localised along basolateral membranes of the epithelium and in the lumen of the mid-caput region. The monolayer in culture was of principal cells only and they maintained their polarity and ultrastructural characteristics, but the height of the cells was reduced compared to that obtained in situ.

Acetylglucosaminidase↗
PubMed1989

Partial and total reconstruction of the nose.

For partial reconstruction of the nose we use an original forehead compound-island flap and the frontotemporal flap of Schmid-Meyer. In repairing the lower two-thirds of the nose we apply our fronto-parieto-auricular flap, which was modified by Orticochea and by Washio. For total reconstruction of the nose, we have abandoned the scalp flap of Converse and substitute in its place the fronto-parieto-occipital flap of Galvao.

Humans↗
PubMed1989

[Reconstruction of the upper lip using the mucosal eversion technic].

A one-stage reconstruction technique for upper lip defects is presented. This technique uses everted mucosal advancement flaps for the reconstruction of the vermilion border of the lip. It is an extension of Meyer's method employed in the reconstruction of the lower lip (1973) and differs from Camille Bernard's procedure in that horizontal incisions with bilateral triangular skin excisions are used instead of the more unaesthetic perpendicular incisions in the nasolabial fold, leaving a scar along the vermilion border. The method is suitable for reconstructing the upper lip after excision of up to 80% of its substance, and is superior to traditional methods in terms of both the functional and aesthetic results obtained.

Adult↗
PubMed1989

Platelet specific alloantigens on the platelet glycoprotein Ia/IIa complex.

The majority of platelet alloantigens are located on platelet glycoproteins IIb/IIIa. This report describes a codominant allelic system carried on the glycoprotein Ia/IIa complex, which we originally designated as Zava/Zavb but which is identical to the Bra/Brb system. Furthermore Zava was found to be identical to Hca. The alloantigens could not be detected using a direct binding enzyme immunoassay (EIA) with intact platelets, but were readily detected using a glycoprotein capture EIA and by radioimmunoprecipitation techniques. The two index cases (designated as homozygous Zava and Zavb) had alloantibodies against the corresponding antigen and did not react with their own platelets. Using these alloantibodies and a monoclonal antibody that reacts with the platelet glycoprotein Ia/IIa complex (12F1), we demonstrated that all Ia/IIa molecules carry either Zava or Zavb and we found that Zava and Zavb are on discrete populations of Ia/IIa. Following immunodepletion using either anti-Zava or anti-Zavb, all detectable Ia/IIa complexes from the respective homozygous platelets were removed. Immunodepletion of heterozygous Zava/Zavb with either anti-Zava or anti-Zavb did not reduce the amount of Ia/IIa complexes precipitable using the alternate alloantiserum. Population studies (n = 50) indicated the phenotypic frequency of Zava/Zava is less than 1%; Zava/Zavb is 18% and Zavb/Zavb is 82%. Four different alloantisera that had either anti-Zava or anti-Zavb reactivity also carried reactivity against the Baka or Bakb antigens which may suggest an association in the immune response to these alleles.

Adult↗
PubMed1989

A prospective comparison of four techniques for measuring platelet-associated IgG.

We describe a prospective study comparing four different assays for PAIgG. Platelets from patients with a variety of thrombocytopenic disorders were collected into ACD, washed, and the PAIgG then measured using three assays for surface PAIgG. These included: (a) a direct binding assay using 125I-monoclonal anti-IgG (MoAb); (b) a direct binding assay using 125I-staphylococcal protein A (SPA); and (c) a two-stage assay. PAIgG also was measured using an assay for 'total' PAIgG following platelet lysis. The mean +/- SD number of molecules of IgG per platelet on washed platelets from 29 healthy, non-thrombocytopenic controls was: 86 +/- 80 (125I-MoAb); 94 +/- 96 (125I-SPA); 3520 +/- 1890 (two-stage surface assay); and 10,850 +/- 3720 (total PAIgG). A total of 62 different patients with idiopathic thrombocytopenic purpura or thrombocytopenia complicating systematic lupus erythematosus, and 73 different patients with 'non-immune' thrombocytopenia, were tested using each of the four assays. These 'non-immune' thrombocytopenic patients included patients with carcinoma, septicaemia, pre-eclampsia, chronic leukaemia, thrombotic thrombocytopenic purpura, haemolytic uraemic syndrome, acute leukaemia and myelodysplasia. All four assays gave similar results for both the immune and non-immune thrombocytopenic patients. The sensitivity of the assays for the most severely thrombocytopenic patients with immune thrombocytopenia was: MoAb 60%; SPA 88%; two-stage 82%; and 'total' PAIgG 88%. The specificity of the four assays in the non-immune thrombocytopenic patients was 57% 'total' PAIgG; 63% two-stage surface; 25% SPA; 38% MoAb.(ABSTRACT TRUNCATED AT 250 WORDS)

Antibodies, Monoclonal↗
PubMed1989

Site-specific recombination at oriT of plasmid R1162 in the absence of conjugative transfer.

R1162 is efficiently comobilized during conjugative transfer of the self-transmissible plasmid R751. Bacteriophage M13 derivatives that contain two directly repeated copies of oriT, the site on R1162 DNA required in cis for mobilization, were constructed. Phage DNA molecules underwent recombination during infection of Escherichia coli, with the product retaining a single functional copy of oriT. Recombination was strand specific and depended on R1162 gene products involved in mobilization, but did not require the self-transmissible plasmid vector. Two genes were identified, one essential for recombination and the other affecting the frequency of recombination. Recombination of bacteriophage DNA could form the basis of a simple model for some of the events occurring during conjugation without the complexity of a true mating system.

Amino Acid Sequence↗
PubMed1989

[Alar and columellar refinement in rhinoplasty].

This paper emphasizes the value of surgical techniques of nasal sculpturing with special attention to the structural details in primary and secondary rhinoplasty to achieve a perfect aesthetic as well as functional result. The marginal resection of the alae and the columella allows the appropriate reduction adapting the nostril contour to the new proportions of the nose at the end of a rhinoplasty or to equalize the height of the alar rim in cases of harelip nose. In order to perfect the nasal contour, we also introduce cartilaginous onlays into the anterior part of the columella emphasizing the lobulo-columellar double-angle in a harmonious profile. In case of moderate fibrocicatricial retraction of the alar border, a vestibular advancement flap is dissected through the inter-cartilaginous incision and a thin cartilage graft fills the loss of tissue as a spacer. It is then held in place using transalar mattress sutures. Important losses of alar tissue necessitating composite graft are not especially considered in the paper which essentially concerns refinement procedures. The finesse of the latter seems to be indispensable to adapt the alar-tip-columellar complex to the bony structures of the nose and to ensure the harmonious equilibrium of the rhinoplasty.

Esthetics↗
PubMed1989

[Peribuccal esthetic surgery].

This paper concerns the procedures of remodelling the peribuccal area for aesthetic purposes; these techniques may be planned in isolation or as a complement to a face-lift. In our hands these interventions are helpful firstly to correct and excessively deep nasolabial fold and sublabial crease using dermis or fascia grafts. Secondly, are designed to correct the contour of the upper lip either by smoothening the fine vertical folds, excising a fine strip of skin along the cupid's bow associated with dermabrasion or by diminishing the labial height resecting a buffalo-horn shaped cutaneous strip at the nasal vestibular sill and at the base of the columella. The philtrum is sculptured by dissecting the philtral columns. The medial philtral depression is maintained using translabial mattress sutures. Furthermore the upper and lower lips themselves are able to be augmented by means of dermal or fascia grafts or on the contrary thinned by resecting an appropriate horizontal strip of vermilion or mucous membrane, without any lesion of the orbicularis muscle. To eliminate the sadness of sagging buccal angles, we model the subcutaneous tissue of the labial commissure.

Adult↗
PubMed1989

[De novo diabetic glomerulosclerosis in kidney transplants].

Report on 3 cases who had been non-diabetics at the time of cadaver kidney transplantation. After that within 1-5 years diabetes mellitus developed. The patients died 13-14 years after transplantation (9-12 years of diabetes duration). Autopsy revealed typical nodular glomerulosclerosis including diffuse mesangial widening, glomerular aneurysms, exudative lesions, capsular drops, arteriolosclerosis, and in 2 cases with hyalinosis of the vas efferens; moreover, chronic transplant glomerulopathy was found in all cases. Steroid therapy seemed to be responsible for the diabetic state, in 2 cases apparently in combination with disposition.

Diabetic Nephropathies↗
PubMed1989

Comparison of conventional morphometry and image analysis for the solution of histomorphometric problems.

The main differences between conventional morphometry and image analysis for their application in histopathology are pointed out. Conventional morphometry allows the use of contextural informations and a priori knowledge in a convenient manner. This is the most complicated thing in image analysis. The solution of this problem lies on the field of artificial intelligence. On the other hand, image analysis is able to measure densitometrical and textural features, to determine arbitrary quantiles and moments of feature distributions, to match scenes. Therefore the descriptive power of image analysis is much higher than the power of conventional morphometry.

Humans↗
PubMed1989

[Fibrotic fraction and myocardial cell diameter in latent cardiomyopathy].

100 left-ventricular endomyocardial biopsies of 32 patients were studied by means of morphometric and histological methods. No differences were found between patients with normal left ventricular end-diastolic pressure (LVEDP) and with increased LVEDP in the mean diameter of heart muscle cells, the distribution parameters coefficient of variation and kurtosis, and the volume density of the connective tissue. These patients did not show pathological findings of the other functional parameters and the coronary arteries. Statistically significant differences had been proved between patients with different histological diagnosis (post-myocarditic state, chronic-ischemic heart disease) with respect to the mean diameter of the myocytes (p less than 0.001) and the kurtosis of the distribution of myocytes' diameter (p less than 0.05).

Biopsy↗