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Biomedical subjects

R Matre

Publications and source records attributed to R Matre.

At least 181 records · Page 10Linked to original sources
PubMed1981

Localization of the C3b receptors in human renal glomeruli.

Cryostat sections of human kidney were incubated with C3b containing immune complexes. The complexes were horseradish peroxidase (HRP) and F(ab')2 fragments of rabbit IgG anti-HRP incubated in C4-deficient guinea pig serum. The peroxidase activity was revealed histochemically. The sections were studied using light and electron microscopy. The bound complexes were localized to the foot processes of the visceral epithelial cells and to the surface of the parietal epithelial cells facing Bowman's space. No receptors could be demonstrated on the endothelial or mesangial cells.

Antigen-Antibody Complex↗
PubMed1981

Similarities of C3b receptors in human kidney and spleen.

Receptors for C3b (C3R) in human spleen and kidney were studied using haemadsorption to cryostat sections. The indicator cells, ovine erythrocytes (E) coated with rabbit IgM antibody (A) and human C3b (EAC) adhered strongly to the glomeruli in renal tissue and to the white pulp of spleen. Titration experiments showed that avidity of the two populations of C3R was equal. Activity was independent of Ca++ and Mg++. Periodic acid, formaldehyde, high salt concentrations and trypsin abolished, whereas neuraminidase enhanced the activity. Various temperatures and pHs affected the two populations of C3R similarly. The results obtained indicate that the C3R in spleen and kidney are similar.

Animals↗
PubMed1981

In situ identification of mononuclear cells in cutaneous infiltrates in discoid lupus erythematosus, sarcoidosis and secondary syphilis.

The inflammatory mononuclear cell infiltrates observed in lesional skin from patients with discoid lupus erythematosus (DLE), sarcoidosis, and secondary syphilis have been characterized in situ. Immunological markers (human T lymphocyte antigens and receptors for sheep erythrocytes, C3b, C3d and Fc gamma) were studied by using immunofluorescence tests with IgG F (ab')2 preparation of anti-T lymphocyte serum, hemadsorption with tissue sections, and tests with soluble immune complexes of peroxidase. In DLE, T lymphocytes were the dominant cell type. In sarcoidosis, the epithelioid cells, including giant cells, had markers similar to macrophages. The lymphohistiocytic cells consisted mainly of macrophages, some T lymphocytes and a few B lymphocytes. In secondary syphilis (condylomata lata), macrophages and T lymphocytes were the dominant cell types, and relatively few B lymphocytes were detected.

Antigens↗
PubMed1980

A simplified method for the preparation of EAC14.

Sheep erythrocytes (E), sensitized with unheated rabbit antiserum containing IgG antibodies (EA), bound human C4 when heat-inactivated serum was used as the source of complement (EAC). The EA were not agglutinated by antiserum to rabbit C3 and were not lysed in the presence of C4-deficient guinea pig serum. The EAC were agglutinated by antiserum to human C4, lysed by the addition of C4-deficient serum and were reactive in immune adherence. They were not agglutinated by antiserum to human C3. Accordingly, the cells could be designated as EAC14. They were stable and could be stored at 4 degrees C for at least 1 week without haemolysis occurring, and with no loss of reactivity in immune adherence test. E sensitized in the presence of EDTA or with heat-inactivated rabbit serum did not bind C4.

Animals↗
PubMed1980

An improved method for the detection of human T lymphocytes in tissue sections.

The technique of haemadsorption to tissue sections for the detection of T lymphocytes is described in detail. The modification using the closed chamber technique is convenient, sensitive and reproducible. In principle, it parallels the rosette technique using blood lymphocytes in suspensions and AET-treated sheep erythrocytes. Storage of tissue at -25 degrees C did not reduce the activity of the E-receptors in the tissue. The E-receptors were blocked by incubation of the tissue sections with an anti-T lymphocyte serum.

Child↗
PubMed1980

C4 receptors in the human glomeruli.

Glomeruli in cryostat sections of human renal tissues adsorbed sheep erythrocytes sensitized with the fourth component of human complement (C). The receptors had specificity for the C4b fragment of C4, and were uniformly distributed in the glomeruli. The receptors were sensitive to trypsin and to periodic acid and were blocked in vivo and in vitro by C3 and C4. Receptors in fetal and adult renal tissues and in areas outside in vivo deposits of C in biopsy specimens had similar avidity. Further results suggested that the receptors for C4b are identical to the receptors for C3b, or that the receptors are located on closely related structures.

Complement C3↗
PubMed1980

Acquired angioedema and hypocompleentemia in a patient with myelofibrosis. Effect of danazol treatment.

This paper reports the findings of an "acquired" hereditary angioedema-like syndrome in a patient with myelofibrosis. No previous personal or family history of angioedema was present. The serum complement pattern showed a marked reduction of Cl esterase inhibitor, Clq and C4. All family members had a normal complement profile. Because of frequent attacks of laryngeal angioedema, prophylactic treatment with danazol was started. A striking clinical response was observed as well as a normalizing effect on the underlying biochemical abnormality.

Aged↗
PubMed1979

Fc gamma receptors receptors on fetal rat brain cells during neoplastic transformation in culture after exposure to ethylnitrosourea in vivo.

Fetal rat brain cells, undergoing neoplastic transformation in long-term culture after a single transplacental pulse of N-ethyl-N-nitrosourea on the 18th day of gestation, were investigated for Fc gamma receptors. Rosette assay with cells in suspension and the hemadsorption to cells on coverglasses were used for their detection. The receptors were found on cells after about 2 months in culture. A maximum of 20% of the cells were Fc gamma receptor-positive after 3 months. About 3 weeks later no Fc gamma receptors could be detected. Malignant rat neurogenic cell lines were also negative.

Animals↗
PubMed1979

Lymphocyte subpopulations in thymus and blood from patients with myasthenia gravis.

Cell suspensions were prepared from hyperplastic thymic tissue and lymphoepithelioma from patients with myasthenia gravis and from presumed normal thymic tissue obtained at cardiac surgery. The mononuclear cells were examined for surface markers. The mean percentages of both T lymphocytes and Fc receptor-carrying lymphocytes were similar in the three groups, whereas there was an increase in C receptor-carrying lymphocytes in the samples from myasthenic patients. Sections from the thymus gland were examined for T and B markers. In the hyperplastic thymus and in lymphoepithelioma, the T lymphocytes were distributed diffusely throughout the cortex and the medulla; in the normal thymus they were predominant in the cortex. The mean percentage of T and B lymphocytes in peripheral blood from patients with myasthenia gravis was normal. Thymectomy involved a transitory decrease in T lymphocytes with a corresponding increase in B lymphocytes.

B-Lymphocytes↗
PubMed1979

Complement receptors in human peripheral nerve tissue.

Cryostat sections of human peripheral nerve tissue adsorbed sheep erythrocytes sensitized with antibody and human complement (EAC). Activated complement was essential for the reaction to occur. The receptor had specificity for the C3b fragment. In order to obrain binding to nerve tissue, the indicator cells had to be coated with more complement than that required for binding to C3b receptors in spleen and renal tissue. The receptors are located within the nerve fascicles and are probably of glycoprotein nature. The receptors for C3b in peripheral nerve tissus may be of significance in the deposition of immune complexes, which may play a role in acute polyradiculoneuritis.

Complement Activation↗
PubMed1979

A study of Sézary cells in peripheral blood and skin lesions.

A patient with Sézary syndrome is presented. By phase contrast and scanning electron microscopy Sézary cells were demonstrated in peripheral blood and in skin tissue. Investigation of atypical mononuclear cells seen in the peripheral blood showed that these cells lack receptors for sheep erythrocytes. C3 and Fcgamma. Similarly, an examination of dermal mononuclear cell infiltrates showed that most of the cells lack receptors for sheep erythrocytes, C3 and Fcgamma.

Aged↗
PubMed1978

Characterization of mononuclear cell infiltrates in psoriatic lesions.

The dermal mononuclear cell infiltrates of psoriatic lesions were characterized by receptors for sheep erythrocytes (T-lymphocytes), C3b receptors (macrophages and B-lymphocytes) and C3d receptors (B-lymphocytes), using hemadsorption to cryostat sections in a closed chamber. T-lymphocytes and macrophages were the predominant inflammatory cells. Very few B-lymphocytes were detected. These findings are discussed in relation to the possible pathogenetic signficance of cellular immunity in psoriasis.

B-Lymphocytes↗
PubMed1978

Complement receptors in pathological human renal glomeruli.

Glomerular complement receptors (GCR) were demonstrated by the adsorption of C3 coated sheep erythrocytes to cryostat sections of human kidneys. An epithelial localization of the GCR is implied by the binding pattern of the indicator cells. The GCR activity was reduced in all glomeruli where in vivo deposition of immunoglobulin and C3 along the capillary wall was observed. In fourteen out of nineteen biopsies where deposits in the mesangium were seen, reduced GCR activity was also observed, whereas GCR activity was normal in the other five. Immunofluorescence and haemadsorption tests performed on the same section showed that C3 deposits corresponded to areas showing reduced complement receptor activity. Areas outside the C3 deposits adsorbed indicator cells coated with various amounts of C3 similarly to the adsorption to glomeruli seen in normal adult and fetal kidneys. Sclerotic glomeruli showed no GCR activity. The results indicate that the complement receptors play a role for the binding of complement-containing complexes in glomeruli.

Binding Sites, Antibody↗
PubMed1978

The placental Fcgamma receptors studied using immune complexes of peroxidase.

Immune complexes of horseradish peroxidase (HRP) and rabbit IgG antibodies to HRP were used to study the Fcgamma receptors in normal human placenta. Cryostat sections of placental tissue were incubated with the complexes, and the peroxidase activity was revealed histochemically. The bound complexes were localized to the apical surface of the trophoblasts and endothelial cells of the fetal stem vessels. Binding also occurred within the wall of some fetal vessels, to stromal cells and occasionally to areas corresponding to the trophoblastic membrane. The strongest binding was obtained with immune complexes prepared at slight antigen excess. Eight- to sixteen-fold increased concentration of human and rabbit IgG was needed to block the binding of immune complexes. Bovine and porcine IgG did not block the binding. Treatment of tissue sections with neuraminidase enhanced the binding activity of the receptors. The technique is very convenient for studies of Fcgamma receptors in tissue. However, unfixed frozen placental tissue was not suitable for ultrastructural studies.

Animals↗